The role of humanitarian disciplines in professional education

The article considers the possibility of integration of humanitarian and professional training of future specialists by means of humanitarian disciplines (literature, history)В статье рассматриваются возможности интеграции гуманитарной и профессиональной подготовки будущих специалистов средствами гуманитарных дисциплин (литература, история

Historical aspects of training specialists in hairstyling, image design and style

The article analyses historical aspects of vocational training of specialists in the field of hairdressing, image design and style; defines the conditions and prerequisites of development of these branches of service and consumer market; world and Russian systems of vocational education in the sphere of image design are characterizedАнализируются исторические аспекты профессиональной подготовки специалистов в области парикмахерского искусства, дизайна имиджа и стиля; определяются условия и предпосылки развития данных отраслей сервиса и потребительского рынка; характеризуются существующие системы профессионального обучения специалистов в области дизайна имиджа: мировые и российски

The relationship between the parameters of life meaningfulness and responsibility of medical workers

The article presents the results of a theoretical and empirical study of the meaningfulness of life and responsibility of medical workers. The diagnostic procedures used «the test of life-meaning orientations» and «Multidimensional functional diagnostics of responsibility (OTV–110) » [2, 10]. As a result of the study it was revealed that respondents are distinguished by a high level of meaningfulness of life, perceive their own life as productive, focused on achieving goals and effectiveness. It is shown that medical workers have a holistic understanding of the essence and various aspects of responsibility, are serious about performing of difficult and important tasks, react internally to possible failures in their professional activities, are sensitive to the difficulties and problems of other people. Connections of life-meaning orientations and parameters of respondents’ responsibility are established. In conclusion, it is stated that the results of the study may be useful in predicting the improvement of the professional efficiency of medical workers and planning opportunities for their further professional growth

Membrane-cytoskeletal crosstalk mediated by myosin-I regulates adhesion turnover during phagocytosis

Phagocytosis of invading pathogens or cellular debris requires a dramatic change in cell shape driven by actin polymerization. For antibody-covered targets, phagocytosis is thought to proceed through the sequential engagement of Fc-receptors on the phagocyte with antibodies on the target surface, leading to the extension and closure of the phagocytic cup around the target. We find that two actin-dependent molecular motors, class 1 myosins myosin le and myosin if, are specifically localized to Fc-receptor adhesions and required for efficient phagocytosis of antibody-opsonized targets. Using primary macrophages lacking both myosin le and myosin if, we find that without the actin-membrane linkage mediated by these myosins, the organization of individual adhesions is compromised, leading to excessive actin polymerization, slower adhesion turnover, and deficient phagocytic internalization. This work identifies a role for class 1 myosins in coordinated adhesion turnover during phagocytosis and supports a mechanism involving membrane-cytoskeletal crosstalk for phagocytic cup closure

Study of acute and chronic toxicity of feed additive based on vermiculture

The paper presents the results of study of the toxicity of a feed additive, which includes Leuzea safflower (Rhaponticum carthamoides) and protein obtained from vermiculture. The acute toxicity of the feed additive was studied in 31 SD white rats. It has been established that the acute oral toxicity of the feed additive is above 2500 mg/kg, which makes it possible to classify the feed additive as hazard class 3 and classify it as “moderately hazardous” according to GOST 12.1.007-76. The chronic toxicity of the feed additive was studied in 22 SD white rats. The feed additive was administered orally at a dose of 120 and 1200 mg/kg for 30 days. Long-term effects were assessed 14 days after the last introduction of the feed additive. To assess the toxic effects, the effect of the additive on the general condition of the animals, food and water intake was evaluated, blood and urine were analyzed. Complete blood count was carried out by standard methods with the determination of the following parameters: hematocrit, hemoglobin level, the number of erythrocytes, platelets, leukocytes, eosinophils, segmented neutrophils, lymphocytes and monocytes. The study of biochemical parameters of blood was carried out using standard kits for biochemical analysis. The following indicators were determined: ALT, total bilirubin, glucose, creatinine and urea. The urinalysis included the determination of the following indicators: density, leukocytes, nitrites, pH, erythrocytes, protein,  glucose, ascorbic acid, ketones, urobilinogen, bilirubin. There was no statistically significant difference in blood and urine parameters between the animals of the control and experimental groups. A pathohistological examination of the stomach, liver, kidneys and heart was carried out, which showed the absence of a toxic effect of the feed additive. Local irritant action was studied in pathomorphological studies. It has been established that the feed additive does not have an irritating effect at the injection site. Thus, the feed additive can be used in feeding farm animals and birds at the recommended dose

ХИМИЧЕСКИЙ СОСТАВ ЛЕТУЧИХ ЭФИРНЫХ МАСЕЛ, ВЫДЕЛЯЕМЫХ В АТМОСФЕРУ ЛИСТЬЯМИ ПРЕДСТАВИТЕЛЕЙ СЕМЕЙСТВА LAURACEAE JUSS. В ОРАНЖЕРЕЙНОЙ КУЛЬТУРЕ, И ИХ АНТИМИКРОБНАЯ АКТИВНОСТЬ

The composition of leaf volatiles and the antimicrobial activity of essential oils of the representatives of the family Lauraceae Juss. cultured in the hothouse against Bacilus polymyxa (Prazmowski) Mace, Bacillus megaterium de Bary, Staphylococcus saprophyticus Shaw et al, Pseudomonas fluorescens Migula, Pseudomonas putida Trevisan, Escherichia coli Castellani, and Chalmers bacterial strains are studied. It is shown that terpenoids camphor and its fractions are the common components in the composition of volatile compounds of the leaves of the representatives of the family Lauraceae Juss. and its percentage is species-specific. The antimicrobial activity of essential oils determines the dominant components in addition to camphor and its fractions, as compounds having a strong toxic effect against pathogenic bacteria and fungi such as sesquiterpene alpha-cubebene (2.30  %) of Cinnamomum tamala leaves and eugenol (6.22  %) in Laurus azorica leaves.Исследован состав летучих компонентов листьев и антимикробная активность их эфирных масел у представителей сем. Lauraceae Juss., культивируемых в оранжереях, в отношении бактериальных шаммов: Bacilus polymyxa (Prazmowski) Mace, Bacillus megaterium de Bary, Staphylococcus saprophyticus Shaw et al., Pseudomonas fluorescens Migula, Pseudomonas putida Trevisan, Escherichia coli Castellani and Chalmers. Показано, что терпеноид камфора и ее фракции являются общими компонентами в составе летучих соединений листьев у представителей данного семейства, а их долевое содержание видоспецифично. Помимо доминантных компонентов: камфоры и ее фракций  антимикробную активность эфирных масел листьев определяют такие соединения, обладающие сильным токсическим эффектом в отношении патогенных бактерий и грибов, как сесквитерпен альфа-кубебен (2,30  %) в листьях Cinnamomum tamala и эвгенол (6,22  %) в листьях Laurus azorica

UNC-45a promotes myosin folding and stress fiber assembly

Contractile actomyosin bundles, stress fibers, are crucial for adhesion, morphogenesis, and mechanosensing in nonmuscle cells. However, the mechanisms by which nonmuscle myosin II (NM-II) is recruited to those structures and assembled into functional bipolar filaments have remained elusive. We report that UNC-45a is a dynamic component of actin stress fibers and functions as a myosin chaperone in vivo. UNC-45a knockout cells display severe defects in stress fiber assembly and consequent abnormalities in cell morphogenesis, polarity, and migration. Experiments combining structured-illumination microscopy, gradient centrifugation, and proteasome inhibition approaches revealed that a large fraction of NM-II and myosin-1c molecules fail to fold in the absence of UNC-45a. The remaining properly folded NM-II molecules display defects in forming functional bipolar filaments. The C-terminal UNC-45/Cro1/She4p domain of UNC-45a is critical for NM-II folding, whereas the N-terminal tetratricopeptide repeat domain contributes to the assembly of functional stress fibers. Thus, UNC-45a promotes generation of contractile actomyosin bundles through synchronized NM-II folding and filament-assembly activities.Peer reviewe

Autoantibody levels in blood of <i>H. pylori</i>-infected patients with chronic gastritis

Helicobacter pylori (H. pylori) increases the risk of diseases associated with mucous membrane inflammation of gastrointestinal tract, in particular, gastritis, stomach ulcers, and duodenal ulcers. It may also induce a chronic immune response, causing damage to the mucous membrane and development of these diseases. In addition, the role of H. pylori in the initiation of a wide range of autoimmune diseases is discussed. The aim of this study was to assess the level of autoantibodies – markers of various autoimmune diseases in the blood of H. pylori-infected patients with chronic gastritis. We used samples of whole peripheral blood from 267 primary patients with chronic gastritis in the acute stage. The presence of H. pylori in gastric juice from patients was determined using real-time PCR. The level of autoantibodies to double-stranded and single-stranded DNA, autoantibodies to thyroglobulin, thyroid peroxidase, concentration of rheumatoid factor, IgG autoantibodies to the cyclic citrullinated peptide, IgM and IgG autoantibodies to beta(2)-glycoprotein were determined by the enzyme immunoassay. The average level of rheumatoid factor in blood serum was similar for H. pylori-infected and non-infected patients, and did not exceed the normal values. The level of antibodies to cyclic citrullinated peptide, one of the sensitive markers of rheumatoid arthritis, was increased in all patients, being, however, significantly lower in H. pylori-infected patients compared with non-infected persons. Autoantibodies to thyroglobulin, thyroid peroxidase are considered classic markers of autoimmune diseases of the thyroid gland. In blood of H. pylori-infected patients we have found an increased concentration of autoantibodies to thyroglobulin and thyroid peroxidase in comparison with non-infected ones, but the average level of these antibodies did not exceed the normal range. Any differences in the levels of systemic lupus erythematosus serological markers, i.e., autoantibodies to double-stranded and single-stranded DNA, were found between H. pylori-infected and non-infected patients. The levels of thrombosis risk marker in patients with systemic lupus erythematosus (IgG and IgM autoantibodies to beta(2)-glycoprotein) were also within the normal ranges. However, in H. pylori-infected patients, it even turned out to be statistically significantly lower than in non-infected ones. Thus, no data have been obtained on increased levels of the tested markers of autoimmune pathology in blood of H. pylori-infected patients with chronic gastritis at the acute stage. However, this does not allow us to make an unambiguous conclusion that the influence of H. pylori does not affect the development of immunological changes associated with autoimmune diseases

Assembly and function of the Photosystem II manganese stabilizing protein: lessons from its natively unfolded behavior

The Photosystem II (PS II) manganese stabilizing protein (MSP) possesses characteristics, including thermostability, ascribed to the natively unfolded class of proteins (Lydakis-Simantiris et al. (1999) Biochemistry 38: 404–414). A site-directed mutant of MSP, C28A, C51A, which lacks the -S–S- bridge, also binds to PS II at wild-type levels and reconstitutes oxygen evolution activity [Betts et al. (1996) Biochim Biophys Acta 1274: 135–142], although the mutant protein is even more disordered in solution. Both WT and C28A, C51A MSP aggregate upon heating, but an examination of the effects of protein concentration and pH on heat-induced aggregation showed that each MSP species exhibited greater resistance to aggregation at a pH near their p I (5.2) than do either bovine serum albumin (BSA) or carbonic anhydrase, which were used as model water soluble proteins. Increases in pH above the p I of the MSPs and BSA enhanced their aggregation resistance, a behavior which can be predicted from their charge (MSP) or a combination of charge and stabilization by -S–S- bonds (BSA). In the case of aggregation resistance by MSP, this is likely to be an important factor in its ability to avoid unproductive self-association reactions in favor of formation of the protein–protein interactions that lead to formation of the functional oxygen evolving complex.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/43544/1/11120_2004_Article_7759.pd