Intramolecular hydrogen bonding of (+)-biotin and biotin derivatives in organic solvents

Copyright © ARKAT USA, IncThe document attached has been archived with permission from the publisher.The intramolecular hydrogen bonding of (+)-biotin and biotin derivatives in mixtures of CDCl3 and DMSO-d6 was investigated by 1H NMR spectroscopy. The dynamic changes observed in the chemic al shifts for the 1-NH and 3-NH protons with changes in solvent composition and temperature confirmed the presence of an intramolecular hydrogen bond between the valeryl side chain hydrogen bond acceptor and the 3-NH proton in a range of biotin derivatives.Geoffrey T. Crisp and Yu-Lin Jian

Deuterium on Venus: Observations from Earth

In view of the importance of the deuterium-to-hydrogen ratio in understanding the evolutionary scenario of planetary atmospheres and its relationship to understanding the evolution of our own Earth, we undertook a series of observations designed to resolve previous observational conflicts. We observed the dark side of Venus in the 2.3 micron spectral region in search of both H2O and HDO, which would provide us with the D/H ratio in Venus' atmosphere. We identified a large number of molecular lines in the region, belonging to both molecules, and, using synthetic spectral techniques, obtained mixing ratios of 34 plus or minus 10 ppm and 1.3 plus or minus 0.2 ppm for H2O and HDO, respectively. These mixing ratios yield a D/H ratio for Venus of D/H equals 1.9 plus or minus 0.6 times 10 (exp 12) and 120 plus or minus 40 times the telluric ratio. Although the detailed interpretation is difficult, our observations confirm that the Pioneer Venus Orbiter results and establish that indeed Venus had a period in its early history in which it was very wet, perhaps not unlike the early wet period that seems to have been present on Mars, and that, in contrast to Earth, lost much of its water over geologic time

First year student expectations: Results from a university-wide student survey

Although much has been written on the first-year experience of students at higher education institutions, less attention has been directed to the expectations of students when they enter an institution for the first time. This paper provides additional insights into the expectations of students at an Australian university and highlights areas in which students’ expectations may not necessarily align with the realities of common university practices. By providing opportunities for students to articulate their expectations, staff are able to use the responses for a constructive dialogue and work towards a more positive alignment between perceived expectations and levels of student satisfaction with their experience.Geoffrey Crisp, Edward Palmer, Deborah Turnbull, Ted Nettelbeck, Lynn Ward, Amanda LeCouteur, Aspa Sarris, Peter Strelan, and Luke Schneide

Spaceborne radar observations: A guide for Magellan radar-image analysis

Geologic analyses of spaceborne radar images of Earth are reviewed and summarized with respect to detecting, mapping, and interpreting impact craters, volcanic landforms, eolian and subsurface features, and tectonic landforms. Interpretations are illustrated mostly with Seasat synthetic aperture radar and shuttle-imaging-radar images. Analogies are drawn for the potential interpretation of radar images of Venus, with emphasis on the effects of variation in Magellan look angle with Venusian latitude. In each landform category, differences in feature perception and interpretive capability are related to variations in imaging geometry, spatial resolution, and wavelength of the imaging radar systems. Impact craters and other radially symmetrical features may show apparent bilateral symmetry parallel to the illumination vector at low look angles. The styles of eruption and the emplacement of major and minor volcanic constructs can be interpreted from morphological features observed in images. Radar responses that are governed by small-scale surface roughness may serve to distinguish flow types, but do not provide unambiguous information. Imaging of sand dunes is rigorously constrained by specific angular relations between the illumination vector and the orientation and angle of repose of the dune faces, but is independent of radar wavelength. With a single look angle, conditions that enable shallow subsurface imaging to occur do not provide the information necessary to determine whether the radar has recorded surface or subsurface features. The topographic linearity of many tectonic landforms is enhanced on images at regional and local scales, but the detection of structural detail is a strong function of illumination direction. Nontopographic tectonic lineaments may appear in response to contrasts in small-surface roughness or dielectric constant. The breakpoint for rough surfaces will vary by about 25 percent through the Magellan viewing geometries from low to high Venusian latitudes. Examples of anomalies and system artifacts that can affect image interpretation are described

Nerve-targeted probes for fluorescence-guided intraoperative imaging.

A fundamental goal of many surgeries is nerve preservation, as inadvertent injury can lead to patient morbidity including numbness, pain, localized paralysis and incontinence. Nerve identification during surgery relies on multiple parameters including anatomy, texture, color and relationship to surrounding structures using white light illumination. We propose that fluorescent labeling of nerves can enhance the contrast between nerves and adjacent tissue during surgery which may lead to improved outcomes. Methods: Nerve binding peptide sequences including HNP401 were identified by phage display using selective binding to dissected nerve tissue. Peptide dye conjugates including FAM-HNP401 and structural variants were synthesized and screened for nerve binding after topical application on fresh rodent and human tissue and in-vivo after systemic IV administration into both mice and rats. Nerve to muscle contrast was quantified by measuring fluorescent intensity after topical or systemic administration of peptide dye conjugate. Results: Peptide dye conjugate FAM-HNP401 showed selective binding to human sural nerve with 10.9x fluorescence signal intensity (1374.44 ± 425.96) compared to a previously identified peptide FAM-NP41 (126.17 ± 61.03). FAM-HNP401 showed nerve-to-muscle contrast of 3.03 ± 0.57. FAM-HNP401 binds and highlight multiple human peripheral nerves including lower leg sural, upper arm medial antebrachial as well as autonomic nerves isolated from human prostate. Conclusion: Phage display has identified a novel peptide that selectively binds to ex-vivo human nerves and in-vivo using rodent models. FAM-HNP401 or an optimized variant could be translated for use in a clinical setting for intraoperative identification of human nerves to improve visualization and potentially decrease the incidence of intra-surgical nerve injury

Light storage protocols in Tm:YAG

We present two quantum memory protocols for solids: A stopped light approach based on spectral hole burning and the storage in an atomic frequency comb. These procedures are well adapted to the rare-earth ion doped crystals. We carefully clarify the critical steps of both. On one side, we show that the slowing-down due to hole-burning is sufficient to produce a complete mapping of field into the atomic system. On the other side, we explain the storage and retrieval mechanism of the Atomic Frequency Comb protocol. This two important stages are implemented experimentally in Tm$^{3+}$- doped yttrium-aluminum-garnet crystal