Estuarine Macrophytes at Bakkhali, Cox’s Bazar, Bangladesh with Reference to Mangrove Diversity

The diversity and ecosystem significance of macrophyte communities was studied, with emphasis on the mangrove species in the Bakkhali estuary of Cox’s Bazar, Bangladesh. Macrophytes of the Bakkhali river estuary were mainly mangroves (Avicennia marina Forssk Vierh and A. alba Blume and Acanthus ilicifolius Linnaeus) of inundation Class-IV; i.e., the mangroves are inundated 2-20 times per month (Watson, 1928), a wild rice variety of salt marsh (Porteresia coarctata Tateoka), Congon grass (Imperata cylindrica P. Beauv.), seagrass (Halophila beccarii Ascherson), macro-algae (Ulva intestinalis Linnaeus, Catenella nipae Zanardini and Hypnea sp.) and mangrove associates Suaeda maritima Only two individual plants of mangrove, Sonneratia apetala and Aegialitis rotundifolia, were found growing in the inter-tidal area of this estuary. On the eastern side of the estuary, almost 100% of the intertidal area is covered by the salt marsh plant P. coarctata, with patches of seagrass Halophila beccarii. The western portion of the estuary is covered by mangroves, mixed with patchy salt marsh and seagrass in and sheltered by a sand bar. Salt marsh and seagrass grow in mangrove dominated sites as 2nd pioneer species in the newly accreted land at the mouth of Bakkhali estuary. These estuarine macrophytes are of both economic and ecological significance and the macrophyte community plays a principal role directly or indirectly in supporting the local communities as source of food, cash and energy

Validating the Measurement of Social Capital in Bangladesh: A Cognitive Approach

Despite the growing evidence linking social capital to improvements in health and health behaviors, reliable measures of social capital are lacking in low-income countries. To accurately measure social capital in new contexts, there is a need to validate social capital survey questions in each new cultural setting. In this article we examine the content validity of the measurement of social capital in Bangladesh using qualitative methods. In December 2012, we conducted four focus group discussions and 32 cognitive interviews in one rural subdistrict (Durgapur) and one urban slum (Mirpur). We used the findings from the focus groups and cognitive interviews to create a new social capital survey instrument that can be used by health and development organizations in Bangladesh. Furthermore, in this article we provide insight into social capital survey research in general, including suggestions for the measurement of group membership, social support, collective action, and social trust

Estuarine macrophytes at Bakkhali, Cox's Bazar, Bangladesh with reference to mangrove diversity

The diversity and ecosystem significance of macrophyte communities was studied, with emphasis on the mangrove species in the Bakkhali estuary of Cox’s Bazar, Bangladesh. Macrophytes of the Bakkhali river estuary were mainly mangroves (Avicennia marina Forssk Vierh and A. alba Blume and Acanthus ilicifolius Linnaeus) of inundation Class-IV; i.e., the mangroves are inundated 2-20 times per month (Watson, 1928), a wild rice variety of salt marsh (Porteresia coarctata Tateoka), Congon grass (Imperata cylindrica P. Beauv.), seagrass (Halophila beccarii Ascherson), macro-algae (Ulva intestinalis Linnaeus, Catenella nipae Zanardini and Hypnea sp.) and mangrove associates Suaeda maritima Only two individual plants of mangrove, Sonneratia apetala and Aegialitis rotundifolia, were found growing in the inter-tidal area of this estuary. On the eastern side of the estuary, almost 100% of the intertidal area is covered by the salt marsh plant P. coarctata, with patches of seagrass Halophila beccarii. The western portion of the estuary is covered by mangroves, mixed with patchy salt marsh and seagrass in and sheltered by a sand bar. Salt marsh and seagrass grow in mangrove dominated sites as 2nd pioneer species in the newly accreted land at the mouth of Bakkhali estuary. These estuarine macrophytes are of both economic and ecological significance and the macrophyte community plays a principal role directly or indirectly in supporting the local communities as source of food, cash and energy

Cytoplasmic chromatin triggers inflammation in senescence and cancer

Chromatin is traditionally viewed as a nuclear entity that regulates gene expression and silencing. However, we recently discovered the presence of cytoplasmic chromatin fragments that pinch off from intact nuclei of primary cells during senescence, a form of terminal cell-cycle arrest associated with pro-inflammatory responses. The functional significance of chromatin in the cytoplasm is unclear. Here we show that cytoplasmic chromatin activates the innate immunity cytosolic DNA-sensing cGAS-STING (cyclic GMP-AMP synthase linked to stimulator of interferon genes) pathway, leading both to short-term inflammation to restrain activated oncogenes and to chronic inflammation that associates with tissue destruction and cancer. The cytoplasmic chromatin-cGAS-STING pathway promotes the senescence-associated secretory phenotype in primary human cells and in mice. Mice deficient in STING show impaired immuno-surveillance of oncogenic RAS and reduced tissue inflammation upon ionizing radiation. Furthermore, this pathway is activated in cancer cells, and correlates with pro-inflammatory gene expression in human cancers. Overall, our findings indicate that genomic DNA serves as a reservoir to initiate a pro-inflammatory pathway in the cytoplasm in senescence and cancer. Targeting the cytoplasmic chromatin-mediated pathway may hold promise in treating inflammation-related disorders

Incidence of Diabetes in the Working Population in Spain: Results from the ICARIA Cohort

INTRODUCTION: Our objective was to evaluate the incidence of type 2 diabetes mellitus (T2DM) in a working population in Spain and to assess associations between its development and several risk factors. METHODS: The ICARIA (Ibermutuamur CArdiovascular RIsk Assessment) cohort (n = 627,523) includes ~3% of Spanish workers. This analysis was undertaken in individuals whose glycaemic status during the index period (May 2004-December 2007) was determined to be normal or indicative of prediabetes [fasting plasma glucose (FPG) 100-125 mg/dl] and who had at least one FPG measurement taken 9 months after a first measurement during follow-up (May 2004-June 2014) (n = 380,366). T2DM patients were defined as those with an FPG ? 126 mg/day and those who had already been diagnosed with T2DM or were taking antihyperglycaemic medications. RESULTS: The incidence rate of T2DM was 5.0 [95% confidence interval (CI) 4.9-5.1] cases per 1000 person-years. Under multivariate logistic regression analysis, the factor showing the strongest association with the occurrence of T2DM was the baseline FPG level, with the likelihood of T2DM almost doubling for every 5 mg/dl increase in baseline FPG between 100 and < 126 mg/dl. The presence of other cardiometabolic risk factors and being a blue-collar worker were also significantly associated with the occurrence of T2DM. CONCLUSIONS: The incidence of T2DM in the working population was within the range encountered in the general population and prediabetes was found to be the strongest risk factor for the development of diabetes. The workplace is an appropriate and feasible setting for the assessment of easily measurable risk factors, such as the presence of prediabetes and other cardiometabolic factors, to facilitate the early detection of individuals at higher risk of diabetes and the implementation of diabetes prevention programmes

Spontaneous hyaline cartilage regeneration can be induced in an osteochondral defect created in the femoral condyle using a novel double-network hydrogel

<p>Abstract</p> <p>Background</p> <p>Functional repair of articular osteochondral defects remains a major challenge not only in the field of knee surgery but also in tissue regeneration medicine. The purpose is to clarify whether the spontaneous hyaline cartilage regeneration can be induced in a large osteochondral defect created in the femoral condyle by means of implanting a novel double-network (DN) gel at the bottom of the defect.</p> <p>Methods</p> <p>Twenty-five mature rabbits were used in this study. In the bilateral knees of each animal, we created an osteochondral defect having a diameter of 2.4-mm in the medial condyle. Then, in 21 rabbits, we implanted a DN gel plug into a right knee defect so that a vacant space of 1.5-mm depth (in Group I), 2.5-mm depth (in Group II), or 3.5-mm depth (in Group III) was left. In the left knee, we did not apply any treatment to the defect to obtain the control data. All the rabbits were sacrificed at 4 weeks, and the gross and histological evaluations were performed. The remaining 4 rabbits underwent the same treatment as used in Group II, and real-time PCR analysis was performed at 4 weeks.</p> <p>Results</p> <p>The defect in Group II was filled with a sufficient volume of the hyaline cartilage tissue rich in proteoglycan and type-2 collagen. The Wayne's gross appearance and histology scores showed that Group II was significantly greater than Group I, III, and Control (p < 0.012). The relative expression level of type-2 collagen, aggrecan, and SOX9 mRNAs was significantly greater in Group II than in the control group (p < 0.023).</p> <p>Conclusions</p> <p>This study demonstrated that spontaneous hyaline cartilage regeneration can be induced <it>in vivo </it>in an osteochondral defect created in the femoral condyle by means of implanting the DN gel plug at the bottom of the defect so that an approximately 2-mm deep vacant space was intentionally left in the defect. This fact has prompted us to propose an innovative strategy without cell culture to repair osteochondral lesions in the femoral condyle.</p

What constitutes responsiveness of physicians: A qualitative study in rural Bangladesh

Responsiveness entails the social actions by health providers to meet the legitimate expectations of patients. It plays a critical role in ensuring continuity and effectiveness of care within people centered health systems. Given the lack of contextualized research on responsiveness, we qualitatively explored the perceptions of outpatient users and providers regarding what constitute responsiveness in rural Bangladesh. An exploratory study was undertaken in Chuadanga, a southwestern Bangladeshi District, involving in-depth interviews of physicians (n = 17) and users (n = 7), focus group discussions with users (n = 4), and observations of patient provider interactions (three weeks). Analysis was guided by a conceptual framework of responsiveness, which includes friendliness, respecting, informing and guiding, gaining trust and optimizing benefits. In terms of friendliness, patients expected physicians to greet them before starting consultations; even though physicians considered this unusual. Patients also expected physicians to hold social talks during consultations, which was uncommon. With regards to respect patients expected physicians to refrain from disrespecting them in various ways; but also by showing respect explicitly. Patients also had expectations related to informing and guiding: they desired explanation on at least the diagnosis, seriousness of illness, treatment and preventive steps. In gaining trust, patients expected that physicians would refrain from illegal or unethical activities related to patients, e.g., demanding money against free services, bringing patients in own private clinics by brokers (dalals), colluding with diagnostic centers, accepting gifts from pharmaceutical representatives. In terms of optimizing benefits: patients expected that physicians should be financially sensitive and consider individual need of patients. There were multiple dimensions of responsiveness- for some, stakeholders had a consensus; context was an important factor to understand them. This being an exploratory study, further research is recommended to validate the nuances of the findings. It can be a guideline for responsiveness practices, and a tipping point for future research

Gene expression profile of the cartilage tissue spontaneously regenerated in vivo by using a novel double-network gel: Comparisons with the normal articular cartilage

<p>Abstract</p> <p>Background</p> <p>We have recently found a phenomenon that spontaneous regeneration of a hyaline cartilage-like tissue can be induced in a large osteochondral defect by implanting a double-network (DN) hydrogel plug, which was composed of poly-(2-Acrylamido-2-methylpropanesulfonic acid) and poly-(N, N'-Dimetyl acrylamide), at the bottom of the defect. The purpose of this study was to clarify gene expression profile of the regenerated tissue in comparison with that of the normal articular cartilage.</p> <p>Methods</p> <p>We created a cylindrical osteochondral defect in the rabbit femoral grooves. Then, we implanted the DN gel plug at the bottom of the defect. At 2 and 4 weeks after surgery, the regenerated tissue was analyzed using DNA microarray and immunohistochemical examinations.</p> <p>Results</p> <p>The gene expression profiles of the regenerated tissues were macroscopically similar to the normal cartilage, but showed some minor differences. The expression degree of COL2A1, COL1A2, COL10A1, DCN, FMOD, SPARC, FLOD2, CHAD, CTGF, and COMP genes was greater in the regenerated tissue than in the normal cartilage. The top 30 genes that expressed 5 times or more in the regenerated tissue as compared with the normal cartilage included type-2 collagen, type-10 collagen, FN, vimentin, COMP, EF1alpha, TFCP2, and GAPDH genes.</p> <p>Conclusions</p> <p>The tissue regenerated by using the DN gel was genetically similar but not completely identical to articular cartilage. The genetic data shown in this study are useful for future studies to identify specific genes involved in spontaneous cartilage regeneration.</p

Sukob radnih i obiteljskih uloga i zadovoljstva u medicinskih sestara u Hrvatskoj s različitim sustavima smjenskoga rada

The objective of this study was to examine the perception of conflict between work and family roles and job, family, and life satisfaction among nurses in Croatia. One hundred and twenty-nine nurses (married mothers) working in hospitals in Zadar, Šibenik, and Split were divided in four groups according to their worktime schedule. The participants completed a survey, which included a set of sociodemographic-type questions, questions about the level and allocation of family responsibilities between spouses, and scales measuring the perceived negative effects of worktime, psychological demands of the work, work-family conflict, and semantic differential scales for measuring the affective and cognitive-evaluative component of job, family, and life satisfaction. This was the first study in Croatia to deal with work-family conflict among nurses or workers with different shift systems. The results of this study indicate that nurses working morning shifts only experienced less conflict between work and family than other groups of nurses, who worked the morning, afternoon, and the night shift. The cognitive-evaluative component of job satisfaction was the highest among morning shift nurses and the lowest in nurses who worked 12-hour shifts, while the affective component of life satisfaction was the lowest in nurses working irregular and backward rotated shifts. These results confi rm that shiftwork makes the work-family role conflict even worse. They also support the view that the type of shift rotation matters.Cilj je ovoga istraživanja bio ispitati percepciju sukoba između radnih i obiteljskih uloga i radno, obiteljsko i životno zadovoljstvo kod medicinskih sestara s obzirom na različite vrste organizacije radnoga vremena. 129 udatih medicinskih sestara majki koje rade u bolnicama u Zadru, Šibeniku i Splitu podijeljeno je u 4 skupine s obzirom na vrstu organizacije radnoga vremena. Sudionice su ispunile upitnik koji je uključivao set pitanja sociodemografskoga tipa, pitanja o količini i raspodjeli obiteljskih odgovornosti među supružnicima i skale koje mjere percipirane negativne efekte radnoga vremena, psihološke zahtjeve posla, sukob između radne i obiteljske uloge te skale semantičkoga diferencijala za mjerenje afektivne i kognitivnoevaluativne komponente radnog i obiteljskog zadovoljstva i života općenito. Ovo je prvo istraživanje provedeno u Hrvatskoj u kojem je ispitivan sukob radne i obiteljske uloge kod medicinskih sestara ili općenito radnika s različitim sustavima smjenskoga rada. Rezultati pokazuju da sestre koje rade u stalnim jutarnjim smjenama doživljavaju manje sukoba zbog ometajućih utjecaja rada na obitelj od grupe medicinskih sestara koje su radile u tri smjene (u koje je uključena noćna smjena). Kognitivno-evaluativna komponenta zadovoljstva poslom bila je najviša kod medicinskih sestara s jutarnjim smjenama, a najniža kod onih koje su radile u 12-satnim smjenama. Afektivna komponenta zadovoljstva životom bila je najniža kod medicinskih sestara koje su radile u iregularnim i unatrag rotiranim smjenama. Ovi su rezultati dodatan pokazatelj različitih i više ili manje negativnih efekata smjenskoga rada na sukob između radnih i obiteljskih uloga te dodatno upozoravaju na važnost uzimanja u obzir vrste rotacije smjena

Pro-Inflammatory Cytokine Induction of 11β-hydroxysteroid Dehydrogenase Type 1 in A549 Cells Requires Phosphorylation of C/EBPβ at Thr235

11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) converts inert glucocorticoids into active forms, thereby increasing intracellular glucocorticoid levels, important to restrain acute inflammation. 11β-HSD1 is induced by pro-inflammatory cytokines in a variety of cells. Here, we show 11β-HSD1 expression in human A549 epithelial cells is increased by pro-inflammatory cytokines (IL-1α/TNFα) via the P2 promoter of the HSD11B1 gene. Inhibition of p38 MAPK attenuated the pro-inflammatory cytokine induction of mRNA encoding 11β-HSD1 as well as that encoding C/EBPβ. IL-1α/TNFα-induced phosphorylation of C/EBPβ at Thr235 was also attenuated by p38 MAPK inhibition suggesting involvement of a p38 MAPK-C/EBPβ pathway. siRNA-mediated knock-down of C/EBPβ and NF-κB/RelA implicated both transcription factors in the IL-1α/TNFα induction of HSD11B1 mRNA. Transient transfections of HSD11B1 promoter-reporter constructs identified the proximal region of the P2 promoter of HSD11B1 as essential for this induction. IL-1α increased binding of C/EBPβ to the HSD11B1 P2 promoter, but this was not observed for NF-κB/RelA, suggesting indirect regulation by NF-κB/RelA. Ectopic expression of mutant chicken C/EBPβ constructs unable to undergo phosphorylation at the threonine equivalent to Thr235 attenuated the IL-1α-induction of HSD11B1, whereas mimicking constitutive phosphorylation of Thr235 (by mutation to aspartate) increased basal expression of HSD11B1 mRNA without affecting IL-1α-induced levels. These data clearly demonstrate a role for both C/EBPβ and NF-κB/RelA in the pro-inflammatory cytokine induction of HSD11B1 in human epithelial cells and show that p38 MAPK-induced phosphorylation of C/EBPβ at Thr235 is critical in this