Secure Cluster-based Routing using TCSA and Hybrid Security Algorithm for WSN

Wireless Sensor Network (WSN) is operated as a medium to connect the physical and information network of Internet-of-Things (IoT). Energy and trust are two key factors that assist reliable communication over the WSN-IoT. Secure data transmission is considered a challenging task during multipath routing over the WSN-IoT. To address the aforementioned issue, secure routing is developed over the WSN-IoT. In this paper, the Trust-based Crow Search Algorithm (TCSA) is developed to identify the Secure Cluster Heads (SCHs) and secure paths over the network. Further, data security while broadcasting the data packets is enhanced by developing the Hybrid Security Algorithm (HSA). This HSA is a combination of the Advanced Encryption Standard (AES) and Hill Cipher Algorithm (HCA). Therefore, the developed TCSA-HSA avoids malicious nodes during communication which helps to improve data delivery and energy consumption. The performance of the TCSA-HSA method is analyzed using Packet Delivery Ratio (PDR), Packet Loss Ratio (PLR), energy consumption, End to End Delay (EED), and throughput. The existing methods namely Optimal Privacy-Multihop Dynamic Clustering Routing Protocol (OP-MDCRP) and Secure and Energy-aware Heuristic-based Routing (SEHR) are used to evaluate the TCSA-HSA performances. The PDR of TCSA-HSA for 100 nodes is 99.7449%, which is high when compared to the OP-MDCRP and SEHR

Music Recommendation System with User-based and Item-based Collaborative Filtering Technique

Internet and E-commerce are the generators of abundant of data, causing information Overloading.  The problem of information overloading is addressed by Recommendation Systems (RS). RS can provide suggestions about a new product, movie or music etc. This paper is about Music Recommendation System, which will recommend songs to users based on their past history i.e. taste. In this paper we proposed a collaborative filtering technique based on users and items. First user-item rating matrix is used to form user clusters and item clusters. Next these clusters are used to find the most similar user cluster or most similar item cluster to a target user. Finally songs are recommended from the most similar user and item clusters. The proposed algorithm is implemented on the benchmark dataset Last.fm. Results show that the performance of proposed method is better than the most popular baseline method

Role of Association Rule Mining in DNA Microarray Data - A Research

Gene expression data analysis with DNA microarray technology has become a fundamental tool in genomic research. DNA Microarray technology monitors the gene expression levels of thousands of genes at a time under a certain condition makes them suit-able for a quite lot of biological applications. As the Association rules are widely using in market-basket analysis, these rules can also be applied in biological problems. Association rules play an important role in the computational biology. In this paper, how the Association rule mining is important for micro array gene association analysis have been discussed. Though the clustering offers a natural solution to these bio-logical problems by discovering frequent item sets on microarray data and reviewed the different methodologies discussed by the authors on micro array gene expressed data

Evolution of protein-coupled RNA dynamics during hierarchical assembly of ribosomal complexes

Assembly of 30S ribosomes involves the hierarchical addition of ribosomal proteins that progressively stabilize the folded 16S rRNA. Here, we use three-color single molecule FRET to show how combinations of ribosomal proteins uS4, uS17 and bS20 in the 16S 5' domain enable the recruitment of protein bS16, the next protein to join the complex. Analysis of real-time bS16 binding events shows that bS16 binds both native and non-native forms of the rRNA. The native rRNA conformation is increasingly favored after bS16 binds, explaining how bS16 drives later steps of 30S assembly. Chemical footprinting and molecular dynamics simulations show that each ribosomal protein switches the 16S conformation and dampens fluctuations at the interface between rRNA subdomains where bS16 binds. The results suggest that specific protein-induced changes in the rRNA dynamics underlie the hierarchy of 30S assembly and simplify the search for the native ribosome structure

A Computational Investigation on the Connection between Dynamics Properties of Ribosomal Proteins and Ribosome Assembly

Assembly of the ribosome from its protein and RNA constituents has been studied extensively over the past 50 years, and experimental evidence suggests that prokaryotic ribosomal proteins undergo conformational changes during assembly. However, to date, no studies have attempted to elucidate these conformational changes. The present work utilizes computational methods to analyze protein dynamics and to investigate the linkage between dynamics and binding of these proteins during the assembly of the ribosome. Ribosomal proteins are known to be positively charged and we find the percentage of positive residues in r-proteins to be about twice that of the average protein: Lys+Arg is 18.7% for E. coli and 21.2% for T. thermophilus. Also, positive residues constitute a large proportion of RNA contacting residues: 39% for E. coli and 46% for T. thermophilus. This affirms the known importance of charge-charge interactions in the assembly of the ribosome. We studied the dynamics of three primary proteins from E. coli and T. thermophilus 30S subunits that bind early in the assembly (S15, S17, and S20) with atomic molecular dynamic simulations, followed by a study of all r-proteins using elastic network models. Molecular dynamics simulations show that solvent-exposed proteins (S15 and S17) tend to adopt more stable solution conformations than an RNA-embedded protein (S20). We also find protein residues that contact the 16S rRNA are generally more mobile in comparison with the other residues. This is because there is a larger proportion of contacting residues located in flexible loop regions. By the use of elastic network models, which are computationally more efficient, we show that this trend holds for most of the 30S r-proteins

Structural basis for the binding of IRES RNAs to the head of the ribosomal 40S subunit

Some viruses exploit internal initiation for their propagation in the host cell. This type of initiation is facilitated by structured elements (internal ribosome entry site, IRES) upstream of the initiator AUG and requires only a reduced number of canonical initiation factors. An important example are IRES of the virus family Dicistroviridae that bind to the inter-subunit side of the small ribosomal 40S subunit and lead to the formation of elongation-competent 80S ribosomes without the help of any initiation factor. Here, we present a comprehensive functional and structural analysis of eukaryotic-specific ribosomal protein rpS25 in the context of this type of initiation and propose a structural model explaining the essential involvement of rpS25 for hijacking the ribosome

The miR-17/92 cluster: a comprehensive update on its genomics, genetics, functions and increasingly important and numerous roles in health and disease.

The miR-17/92 cluster is among the best-studied microRNA clusters. Interest in the cluster and its members has been increasing steadily and the number of publications has grown exponentially since its discovery with more than 1000 articles published in 2012 alone. Originally found to be involved in tumorigenesis, research work in recent years has uncovered unexpected roles for its members in a wide variety of settings that include normal development, immune diseases, cardiovascular diseases, neurodegenerative diseases and aging. In light of its ever-increasing importance and ever-widening regulatory roles, we review here the latest body of knowledge on the cluster\u27s involvement in health and disease as well as provide a novel perspective on the full spectrum of protein-coding and non-coding transcripts that are likely regulated by its members