Comparison between calculated and measured reverberation times

A qualidade das atividades educacionais depende fortemente das condições acústicas das salas de aula. Este artigo apresenta uma avaliação de dois métodos teóricos de cálculo para o Tempo de Reverberação (TR) em salas de aula, fundamentados na fórmula de Sabine e de Hohmann, Setzer e Wehling. Como objeto de estudo foram utilizadas salas de aula da Universidade Federal do Paraná (UFPR), localizadas no Campus III, Setor de Tecnologia, Bloco IV, situadas na cidade de Curitiba, Brasil. Para corroborar os valores encontrados com a aplicação das fórmulas, foram realizadas medições de TR in situ. Os resultados obtidos mostram a Equação de Sabine Modificada (Método Indireto) como a mais indicada, por se aproximar dos valores de tempos de reverberação experimentais.The quality of educational activities strongly depends of the acoustic conditions of classrooms. This article presents an evaluation of two theoretical methods of calculation of the Reverberation Time (RT) in classrooms, based on the formula of Sabine and Hohmann, Setzer and Wehling. This study was carried out in classrooms of the Federal University of the Paraná (UFPR), located in Campus III, Sector of Technology, Block IV, located in the city of Curitiba, Brazil. RT measurements have been made in situ, aiming to corroborate the results obtained form the application of formula,. The results indicate that the modified formulae of Sabine (Indirect Method) is the most suitable one, since the calculated value are the closest ones to the measured reverberation values

Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form

Comparison between calculated and measured reverberation times

A qualidade das atividades educacionais depende fortemente das condições acústicas das salas de aula. Este artigo apresenta uma avaliação de dois métodos teóricos de cálculo para o Tempo de Reverberação (TR) em salas de aula, fundamentados na fórmula de Sabine e de Hohmann, Setzer e Wehling. Como objeto de estudo foram utilizadas salas de aula da Universidade Federal do Paraná (UFPR), localizadas no Campus III, Setor de Tecnologia, Bloco IV, situadas na cidade de Curitiba, Brasil. Para corroborar os valores encontrados com a aplicação das fórmulas, foram realizadas medições de TR in situ. Os resultados obtidos mostram a Equação de Sabine Modificada (Método Indireto) como a mais indicada, por se aproximar dos valores de tempos de reverberação experimentais.The quality of educational activities strongly depends of the acoustic conditions of classrooms. This article presents an evaluation of two theoretical methods of calculation of the Reverberation Time (RT) in classrooms, based on the formula of Sabine and Hohmann, Setzer and Wehling. This study was carried out in classrooms of the Federal University of the Paraná (UFPR), located in Campus III, Sector of Technology, Block IV, located in the city of Curitiba, Brazil. RT measurements have been made in situ, aiming to corroborate the results obtained form the application of formula,. The results indicate that the modified formulae of Sabine (Indirect Method) is the most suitable one, since the calculated value are the closest ones to the measured reverberation values

Towards transparency-encouraging partial software disclosure to enable trust in data usage

Whenever software components process personal or private data, appropriate data protection mechanisms are mandatory. An essential factor in achieving trust and transparency is not to give preference to a single party but to make it possible to audit the data usage in an unbiased way. The scenario in mind for this contribution contains (i) users bringing in sensitive data they want to be safe, (ii) developers building software-based services whose Intellectual Properties (IPs) they desire to protect, and (iii) platform providers wanting to be trusted and to be able to rely on the developers integrity. The authors see these interests as an insufficiently solved field of tension that can be relaxed by a suitable level of transparently represented software components to give insights without exposing every detail

Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form.This work was supported by Goethe University (Corona funds), the DFG-funded CRC: “Molecular Principles of RNA-Based Regulation,” DFG infrastructure funds (project numbers: 277478796, 277479031, 392682309, 452632086, 70653611), the state of Hesse (BMRZ), the Fondazione CR Firenze (CERM), and the IWB-EFRE-program 20007375. This project has received funding from the European Union’s Horizon 2020 research and innovation program under Grant Agreement No. 871037. AS is supported by DFG Grant SCHL 2062/2-1 and by the JQYA at Goethe through project number 2019/AS01. Work in the lab of KV was supported by a CoRE grant from the University of New Hampshire. The FLI is a member of the Leibniz Association (WGL) and financially supported by the Federal Government of Germany and the State of Thuringia. Work in the lab of RM was supported by NIH (2R01EY021514) and NSF (DMR-2002837). BN-B was supported by theNSF GRFP.MCwas supported byNIH (R25 GM055246 MBRS IMSD), and MS-P was supported by the HHMI Gilliam Fellowship. Work in the labs of KJ and KT was supported by Latvian Council of Science Grant No. VPP-COVID 2020/1-0014. Work in the UPAT’s lab was supported by the INSPIRED (MIS 5002550) project, which is implemented under the Action “Reinforcement of the Research and Innovation Infrastructure,” funded by the Operational Program “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014–2020) and cofinanced by Greece and the EU (European Regional Development Fund) and the FP7 REGPOT CT-2011- 285950–“SEE-DRUG” project (purchase of UPAT’s 700MHz NMR equipment). Work in the CM-G lab was supported by the Helmholtz society. Work in the lab of ABö was supported by the CNRS, the French National Research Agency (ANR, NMRSCoV2- ORF8), the Fondation de la Recherche Médicale (FRM, NMR-SCoV2-ORF8), and the IR-RMN-THC Fr3050 CNRS. Work in the lab of BM was supported by the Swiss National Science Foundation (Grant number 200020_188711), the Günthard Stiftung für Physikalische Chemie, and the ETH Zurich. Work in the labs of ABö and BM was supported by a common grant from SNF (grant 31CA30_196256). This work was supported by the ETHZurich, the grant ETH40 18 1, and the grant Krebsliga KFS 4903 08 2019. Work in the lab of the IBS Grenoble was supported by the Agence Nationale de Recherche (France) RA-COVID SARS2NUCLEOPROTEIN and European Research Council Advanced Grant DynamicAssemblies. Work in the CA lab was supported by Patto per il Sud della Regione Siciliana–CheMISt grant (CUP G77B17000110001). Part of this work used the platforms of the Grenoble Instruct-ERIC center (ISBG; UMS 3518 CNRS-CEA-UGA-EMBL) within the Grenoble Partnership for Structural Biology (PSB), supported by FRISBI (ANR-10-INBS-05-02) and GRAL, financed within the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS (ANR-17-EURE- 0003). Work at the UW-Madison was supported by grant numbers NSF MCB2031269 and NIH/NIAID AI123498. MM is a Ramón y Cajal Fellow of the Spanish AEI-Ministry of Science and Innovation (RYC2019-026574-I), and a “La Caixa” Foundation (ID 100010434) Junior Leader Fellow (LCR/BQ/PR19/11700003). Funded by project COV20/00764 fromthe Carlos III Institute of Health and the SpanishMinistry of Science and Innovation to MMand DVL. VDJ was supported by the Boehringer Ingelheim Fonds. Part of this work used the resources of the Italian Center of Instruct-ERIC at the CERM/ CIRMMP infrastructure, supported by the Italian Ministry for University and Research (FOE funding). CF was supported by the Stiftung Polytechnische Gesellschaft. Work in the lab of JH was supported by NSF (RAPID 2030601) and NIH (R01GM123249).Peer reviewe