57 research outputs found

    Baktériumok és glikoprotein antennåk mannózt tartalmazó oligoszacharidjainak szintézise = Synthesis of mannose containing bacterial and glycoprotein oligosaccharides

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    Gram-negatĂ­v baktĂ©riumok sejtfalĂĄban lĂ©vƑ szĂ©nhidrĂĄt komponenseket ĂĄllĂ­tottunk elƑ. MegvalĂłsĂ­tottuk a Bordetella pertussis sejtfalĂĄban talĂĄlhatĂł kĂŒlönleges Ă©pĂ­tƑelem, a 2,3-diacetamido-2,3-didezoxi-D-mannuronsav beta-glikozidjainak szintĂ©zisĂ©t. ElƑállĂ­tottunk nĂ©hĂĄny, Salmonella baktĂ©riumban talĂĄlhatĂł oligoszacharidot Ă©s hĂ­dmolekulĂĄval lĂĄttuk el, neoglikoproteinek preparĂĄlĂĄsa cĂ©ljĂĄbĂłl. Neoglikoproteineket szintetizĂĄltunk kĂŒlönbözƑ mĂłdszerekkel. RĂ©szletesen vizsgĂĄltuk a reduktĂ­v aminĂĄlĂĄs teljesĂ­tƑkĂ©pessĂ©gĂ©t. A szĂ©nhidrĂĄttartalmat MALDI-TOF tömegspektrometriĂĄval hatĂĄroztuk meg. beta-Mannozidokat hoztunk lĂ©tre az oxidĂĄciĂłs-redukciĂłs mĂłdszerrel. SzintetizĂĄltuk az N-glikoprotein antennĂĄk core pentaszacharidjĂĄt glikozil-azid formĂĄjĂĄban. alfa-MannozilezĂ©si reakciĂłkat vizsgĂĄltunk. | Carbohydrate components of the cell-wall of Gram-negative bacteria were synthesized. beta-Glycosides of 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid, a rare building block of the cell-wall of Bordetella pertussis were created. For the preparation of bacterial artificial antigens some spacered oligosaccharides of Salmonella bacteria were synthesized. Neoglycoproteins were obtained by different methodologies. Among them the reductive amination were systematically investigated. The carbohydrate content of the neoglycoproteins were determined by MALDI-TOF mass spectrometry. beta-Mannosides were created by using the oxidation-reduction method. The core pentasaccharide of N-glycans was prepared, as glycosyl azide. The construction of alpha-mannosidic linkages were intensively investigated

    Glycan microarrays : new angles and new strategies

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    Carbohydrate microarrays, comprising hundreds to thousands of different glycan structures on solid surfaces in a spatially discrete pattern, are sensitive and versatile tools for the analysis of glycosylation changes in complex biological samples. Glycoarrays are also suitable for monitoring multiple molecular interactions with biomolecules where sugars are involved, offering a large variety of bioassay options. In this paper we review the most important glycan microarray types currently used with their main applications, and discuss some of the future challenges the technology faces

    Gliko Ă©s foszfopeptidek szintĂ©zisĂ©nek lehetƑsĂ©gei = Synthesis of glyco and phosphopeptides

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    MĂłdszereket dolgoztunk ki oligo-szachariddal glikozilĂĄlt N-glikopeptidek szintĂ©zisĂ©remind konvergens, mind Ă©pĂ­tƑkƑ mĂłdszerrel. VĂ©dƑcsoportkombinĂĄciĂłkat optimalizĂĄltunk O-glikopeptidek szintĂ©zisĂ©re, valamint optimalizĂĄltuk a kapcsolĂĄsi reakciĂłt a glikĂĄn Ă©s a vĂ©dett aminosav között. SzĂĄmos foszfopeptidet Ă©s sejtpenetrĂĄlĂł peptidet szintetizĂĄltunk, rĂ©szben fluoreszcensen jelzett formĂĄban is. Új foldamer molekulĂĄt (N-amino prolin) ĂĄllĂ­tottunk elƑ Ă©s Ă©pĂ­tettĂŒnk be bĂ©ta-aminosav oligomerekbe. MegvizsgĂĄltuk a kapott oligomerek tĂ©rszerkezeti Ă©s stabilitĂĄsviszonyait. | We improved the applicable methods for the synthesis of N-glycopeptides including convergent and building block approach. The protecting group combinations for the synthesis of O-glycopeptides was optimized and we improved the coupling conditions for the reaction of the glycosyl donor and acceptor. Numerous phospho and cell-penetrating peptide were synthesized and a part of them was fluorescently labelled. A new foldamer molecule (N-amino proline) was prepared and incorporated into beta-amino acid oligomers. The conformation and the stability of the new oligomers was investigated

    Phenyl 2,3,4-tri-O-benzyl-1-thio-α-d-mannopyran­oside monohydrate

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    In the title compound, C33H34O5S·H2O, the mannopyran­oside ring adopts a chair conformation with the 2-α-thio­phenyl group occupying an axial position. One of the pendant benzyl groups is disordered over two sets of sites in a 0.5:0.5 ratio. In the crystal, the water mol­ecule makes two O—H⋯O hydrogen bonds to an adjacent sugar mol­ecule with the O atoms of the primary alcohol and ether groups acting as acceptors. At the same time, the OH group of the sugar makes a hydrogen bond to a water mol­ecule

    Identity Politics in Lisa Bellear's Poetry

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    My thesis intends to describe the hard life of Aborigines through the interpretation of Lisa Bellear’s poems. Lisa Bellear was one among the many activists who tried to improve the situation of Aborigines.egyetemiangol nyelv Ă©s irodalo
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