Massive Oe/Be stars at low metallicity: Candidate progenitors of long GRBs?

At low metallicity the B-type stars rotate faster than at higher metallicity, typically in the SMC. As a consequence, it was expected a larger number of fast rotators in the SMC than in the Galaxy, in particular more Be/Oe stars. With the ESO-WFI in its slitless mode, the SMC open clusters were examined and an occurence of Be stars 3 to 5 times larger than in the Galaxy was found. The evolution of the angular rotational velocity seems to be the main key on the understanding of the specific behaviour and of the stellar evolution of such stars at different metallicities. With the results of this WFI study and using observational clues on the SMC WR stars and massive stars, as well as the theoretical indications of long gamma-ray burst progenitors, we identify the low metallicity massive Be and Oe stars as potential LGRB progenitors. Therefore the expected rates and numbers of LGRB are calculated and compared to the observed ones, leading to a good probability that low metallicity Be/Oe stars are actually LGRB progenitors.Comment: poster at IAUS27

Connecting Alternate Practicum Experiences in Teacher Education with the ‘Seven Principles for Good Practice in Undergraduate Education\u27

University students and professors participated in a shared practicum experience with individuals with moderate to severe disabilities through a weekend long fishing tournament sponsored by an Easter Seal’s Camp. Results from open ended questionnaires were inductively analyzed. Results indicate that students felt the experience was beneficial, provided them with the opportunity to work collaboratively with peers and professors, and impacted their perceptions of their professors. Experience is connected with ‘The Seven Principles For Good Practice in Undergraduate Education’ and discussion for implication for practice in teacher education programs is presented

Massive Be and Oe stars at low metallicity and long gamma ray bursts

proceedings of GRBs as probes conference, Como, Italy 2011According to recent theoretical studies, the progenitors of Long Gamma Ray Bursts should be very fast rotating stars, massive enough but not so for collapsing into a black hole. In addition, recent observations seem to show that stars of about 20 solar masses could be at the origin of LGRBs. At low metallicity B-type stars rotate faster than at higher metallicity. We found with the ESO-WFI an occurrence of Be/Oe stars, that are quasi critical rotators, 3 to 5 times larger in the SMC than in the Galaxy. According to our results, and using observational clues on the SMC WR stars, as well as the theoretical predictions of the characteristics must have the LGRB progenitors, we have identified the low metallicity massive Be/Oe stars as potential LGRB progenitors. To support this identification, the expected rates and the numbers of LGRB were then calculated and compared to the observed ones: 3 to 6 LGRBs were found in the local universe in 11 years while 8 were actually observed

Massive Oe/Be stars at low metallicity: candidate progenitors of long GRBs?

At low metallicity B-type stars rotate faster than at higher metallicity, typically in the SMC. As a consequence, a larger number of fast rotators is expected in the SMC than in the Galaxy, in particular more Be/Oe stars. With the ESO-WFI in its slitless mode, we examined the SMC open clusters and found an occurence of Be stars 3 to 5 times larger than in the Galaxy. The evolution of the angular rotational velocity seems to be the main key on the understanding of the specific behaviour and stellar evolution of such stars at different metallicities. With the results of this WFI study and using observational clues on the SMC WR stars and massive stars, as well as the theoretical indications of long gamma-ray burst progenitors, we identify the low metallicity massive Be and Oe stars as potential LGRB progenitors. Therefore the expected rates and numbers of LGRB are calculated and compared to the observed ones, leading to a good probability that low metallicity Be/Oe stars are actually LGRB progenitor

Ashbya Genome Database 3.0: a cross-species genome and transcriptome browser for yeast biologists

BACKGROUND: The Ashbya Genome Database (AGD) 3.0 is an innovative cross-species genome and transcriptome browser based on release 40 of the Ensembl developer environment. DESCRIPTION: AGD 3.0 provides information on 4726 protein-encoding loci and 293 non-coding RNA genes present in the genome of the filamentous fungus Ashbya gossypii. A synteny viewer depicts the chromosomal location and orientation of orthologous genes in the budding yeast Saccharomyces cerevisiae. Genome-wide expression profiling data obtained with high-density oligonucleotide microarrays (GeneChips) are available for nearly all currently annotated protein-coding loci in A. gossypii and S. cerevisiae. CONCLUSION: AGD 3.0 hence provides yeast- and genome biologists with comprehensive report pages including reliable DNA annotation, Gene Ontology terms associated with S. cerevisiae orthologues and RNA expression data as well as numerous links to external sources of information. The database is accessible at

Evolution of the sex-Related Locus and Genomic Features Shared in Microsporidia and Fungi

Microsporidia are obligate intracellular, eukaryotic pathogens that infect a wide range of animals from nematodes to humans, and in some cases, protists. The preponderance of evidence as to the origin of the microsporidia reveals a close relationship with the fungi, either within the kingdom or as a sister group to it. Recent phylogenetic studies and gene order analysis suggest that microsporidia share a particularly close evolutionary relationship with the zygomycetes.Here we expanded this analysis and also examined a putative sex-locus for variability between microsporidian populations. Whole genome inspection reveals a unique syntenic gene pair (RPS9-RPL21) present in the vast majority of fungi and the microsporidians but not in other eukaryotic lineages. Two other unique gene fusions (glutamyl-prolyl tRNA synthetase and ubiquitin-ribosomal subunit S30) that are present in metazoans, choanoflagellates, and filasterean opisthokonts are unfused in the fungi and microsporidians. One locus previously found to be conserved in many microsporidian genomes is similar to the sex locus of zygomycetes in gene order and architecture. Both sex-related and sex loci harbor TPT, HMG, and RNA helicase genes forming a syntenic gene cluster. We sequenced and analyzed the sex-related locus in 11 different Encephalitozoon cuniculi isolates and the sibling species E. intestinalis (3 isolates) and E. hellem (1 isolate). There was no evidence for an idiomorphic sex-related locus in this Encephalitozoon species sample. According to sequence-based phylogenetic analyses, the TPT and RNA helicase genes flanking the HMG genes are paralogous rather than orthologous between zygomycetes and microsporidians.The unique genomic hallmarks between microsporidia and fungi are independent of sequence based phylogenetic comparisons and further contribute to define the borders of the fungal kingdom and support the classification of microsporidia as unusual derived fungi. And the sex/sex-related loci appear to have been subject to frequent gene conversion and translocations in microsporidia and zygomycetes

constant domain exchanged fab enables specific light chain pairing in heterodimeric bispecific seed antibodies

Abstract Background Bispecific antibodies promise to broadly expand the clinical utility of monoclonal antibody technology. Several approaches for heterodimerization of heavy chains have been established to produce antibodies with two different Fab arms, but promiscuous pairing of heavy and light chains remains a challenge for their manufacturing. Methods We have designed a solution in which the CH1 and CL domain pair in one of the Fab fragments is replaced with a CH3-domain pair and heterodimerized to facilitate correct modified Fab-chain pairing in bispecific heterodimeric antibodies based on a strand-exchange engineered domain (SEED) scaffold with specificity for epithelial growth factor receptor and either CD3 or CD16 (FcγRIII). Results Bispecific antibodies retained binding to their target antigens and redirected primary T cells or NK cells to induce potent killing of target cells. All antibodies were expressed at a high yield in Expi293F cells, were detected as single sharp symmetrical peaks in size exclusion chromatography and retained high thermostability. Mass spectrometric analysis revealed specific heavy-to-light chain pairing for the bispecific SEED antibodies as well as for one-armed SEED antibodies co-expressed with two different competing light chains. Conclusion Incorporation of a constant domain-exchanged Fab fragment into a SEED antibody yields functional molecules with favorable biophysical properties. General significance Our results show that the novel engineered bispecific SEED antibody scaffold with an incorporated Fab fragment with CH3-exchanged constant domains is a promising tool for the generation of complete heterodimeric bispecific antibodies with correct light chain pairing

Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii

BACKGROUND: The recently sequenced genome of the filamentous fungus Ashbya gossypii revealed remarkable similarities to that of the budding yeast Saccharomyces cerevisiae both at the level of homology and synteny (conservation of gene order). Thus, it became possible to reinvestigate the S. cerevisiae genome in the syntenic regions leading to an improved annotation. RESULTS: We have identified 23 novel S. cerevisiae open reading frames (ORFs) as syntenic homologs of A. gossypii genes; for all but one, homologs are present in other eukaryotes including humans. Other comparisons identified 13 overlooked introns and suggested 69 potential sequence corrections resulting in ORF extensions or ORF fusions with improved homology to the syntenic A. gossypii homologs. Of the proposed corrections, 25 were tested and confirmed by resequencing. In addition, homologs of nearly 1,000 S. cerevisiae ORFs, presently annotated as hypothetical, were found in A. gossypii at syntenic positions and can therefore be considered as authentic genes. Finally, we suggest that over 400 S. cerevisiae ORFs that overlap other ORFs in S. cerevisiae and for which no homolog can be detected in A. gossypii should be regarded as spurious. CONCLUSIONS: Although, the S. cerevisiae genome is rightly considered as one of the most accurately sequenced and annotated eukaryotic genomes, we have shown that it still benefits substantially from comparison to the completed sequence and syntenic gene map of A. gossypii, an evolutionarily related fungus. This type of approach will strongly support the annotation of more complex genomes such as the human and murine genomes

Waste-derived volatile fatty acids as carbon source for added-value fermentation approaches

The establishment of a sustainable circular bioeconomy requires the effective material recycling from biomass and biowaste beyond composting/fertilizer or anaerobic digestion/bioenergy. Recently, volatile fatty acids attracted much attention due to their potential application as carbon source for the microbial production of high added-value products. Their low-cost production from different types of wastes through dark fermentation is a key aspect, which will potentially lead to the sustainable production of fuels, materials or chemicals, while diminishing the waste volume. This article reviews the utilization of a volatile fatty acid platform for the microbial production of polyhydroxyalkanoates, single cell oil and omega-3 fatty acids, giving emphasis on the fermentation challenges for the efficient implementation of the bioprocess and how they were addressed. These challenges were addressed through a research project funded by the European Commission under the Horizon 2020 programme entitled 'VOLATILE-Biowaste derived volatile fatty acid platform for biopolymers, bioactive compounds and chemical building blocks'.This work was supported by the European project 'Volatile-Biowaste-derived volatile fatty acid platform for biopolymers, bioactive compounds and chemical building blocks' and has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement number 720777