5 research outputs found
Effectiveness of Chest Physiotherapy in Infants Hospitalized with Acute Bronchiolitis: A Multicenter, Randomized, Controlled Trial
Vincent Gajdos and colleagues report results of a randomized trial conducted among hospitalized infants with bronchiolitis. They show that a physiotherapy technique (increased exhalation and assisted cough) commonly used in France does not reduce time to recovery in this population
Reconstruction of the Disassembly Pathway of an Icosahedral Viral Capsid and Shape Determination of Two Successive Intermediates
Viral capsids derived from an icosahedral
plant virus widely used
in physical and nanotechnological investigations were fully dissociated
into dimers by a rapid change of pH. The process was probed in vitro
at high spatiotemporal resolution by time-resolved small-angle X-ray
scattering using a high brilliance synchrotron source. A powerful
custom-made global fitting algorithm allowed us to reconstruct the
most likely pathway parametrized by a set of stoichiometric coefficients
and to determine the shape of two successive intermediates by ab initio
calculations. None of these two unexpected intermediates was previously
identified in self-assembly experiments, which suggests that the disassembly
pathway is not a mirror image of the assembly pathway. These findings
shed new light on the mechanisms and the reversibility of the assembly/disassembly
of natural and synthetic virus-based systems. They also demonstrate
that both the structure and dynamics of an increasing number of intermediate
species become accessible to experiments