Environmental monitoring of Mycobacterium bovis in badger feces and badger sett soil by real-time PCR, as confirmed by immunofluorescence, immunocapture, and cultivation

Real-time PCR was used to detect and quantify Mycobacterium bovis cells in naturally infected soil and badger faeces. Immunomagnetic capture, immunofluorescence and selective culture confirmed species identification and cell viability. These techniques will prove useful for monitoring M. bovis in the environment and for elucidating transmission routes between wildlife and cattle

Physical and social access to land: Spatio-temporal patterns of agricultural expansion

Abstract The expansion of agriculture is posited as one of the main dynamics of land cover change globally, and the robust modeling of these processes is important for policy as well as academic concerns. Madagascar's farmers stand accused as the "proximate agents" of land conversion in one of the world's "hottest" biodiversity hotspots, and numerous studies have been conducted to describe and model the processes by which the country's forests are giving way to agriculture. This paper concerns a relatively small area on the island's eastern escarpment where considerable national and international attention has been paid to slow the expansion of agriculture into the remaining natural forests. The approach adopted here is to begin by examining the degree to which patterns of agricultural conversion can be attributed to a set of factors that have been identified as significant at broader scales in Madagascar and elsewhere, namely topography and prior human settlement and land use patterns. A regression model is constructed, and its predictions compared to the observed land conversion over a 43-year period. The study then examines in detail the spatial patterns highlighted by the failure of the model (the residuals of the regression), breaking the study area into smaller zones, or landscapes. The spatio-temporal trajectories of these zones are then contrasted, with particular attention to the institutional arrangements governing access to land resources. The study finds that while overall land change patterns in the region are largely explained by elevation and village proximity, more specific, sub-regional, trajectories reflect the signatures of institutions governing access to land

Combined resistance and aerobic exercise training reduces insulin resistance and central adiposity in adolescent girls who are obese: randomized clinical trial

Introduction Exercise training is recommended for improving health and protecting against the development of metabolic and cardiovascular pathologies. Combined resistance and aerobic exercise training (CRAE) has been shown to provide unique benefits in older adults with cardiovascular diseases. Purpose We sought to determine the beneficial effects of CRAE in adolescent girls who are obese and hyperinsulinemic. Methods Forty adolescent girls who are obese (age 14.7 ± 1 years; BMI 30 ± 2) were randomly assigned to a “no exercise” (CON n = 20) or combined exercise group (EX n = 20). The EX group performed resistance and aerobic exercise for 12 weeks, 5 times per week. Exercise intensity was increased gradually, from 40 to 70% of heart rate reserve (HRR), every 4 weeks. The brachial-ankle pulse wave velocity (BaPWV), blood pressure (BP), heart rate (HR), blood leptin, adiponectin levels, and body composition were measured before and after the 12-week intervention. Results We observed that CRAE effectively reduced the body fat percentage, body weight, and waist circumference in the EX group (p \u3c 0.05). After 12 weeks of training, subjects in the CRAE group maintained appropriate leptin and adiponectin levels and showed positive improvements of blood insulin, glucose, and insulin resistance parameters relative to baseline and to the CON group (p \u3c 0.05). Conclusion CRAE is a useful therapeutic method to alleviate metabolic risk factors in adolescent girls who are obese and hyperinsulinemic

Population Dynamics Of A Diverse Rodent Assemblage In Mixed Grass-Shrub Habitat, Southeastern Colorado, 1995–2000

We followed seasonal and year-to-year population dynamics for a diverse rodent assemblage in a short-grass prairie ecosystem in southeastern Colorado (USA) for 6 yr. We captured 2,798 individual rodents (range, one to 812 individuals per species) belonging to 19 species. The two most common species, deer mice (Peromyscus maniculatus) and western harvest mice (Reithrodontomys megalotis), generally had population peaks in winter and nadirs in summer; several other murid species demonstrated autumn peaks and spring nadirs; heteromyids were infrequently captured in winter, and populations generally peaked in summer or autumn. Interannual trends indicated an interactive effect between temperature and precipitation. Conditions associated with low rodent populations or population declines were high precipitation during cold periods (autumn and winter) and low precipitation during warm periods (spring and summer). Severity of adverse effects varied by species. Heteromyids, for example, were apparently not negatively affected by the hot, dry spring and summer of 2000. Cross-correlations for the temporal series of relative population abundances between species pairs (which are affected by both seasonal and interannual population dynamics) revealed positive associations among most murids and among most heteromyids, but there were negative associations between murids and heteromyids. These results have important implications for those attempting to model population dynamics of rodent populations for purposes of predicting disease risk

The Pan-STARRS 1 Photometric Reference Ladder, Release 12.0

As of 2012 Jan 21, the Pan-STARRS1 $3\pi$ Survey has observed the 3/4 of the sky visible from Hawaii with a minimum of 2 and mean of 7.6 observations in 5 filters, $g_{\rm P1},r_{\rm P1},i_{\rm P1},z_{\rm P1},y_{\rm P1}$. Now at the end of the second year of the mission, we are in a position to make an initial public release of a portion of this unprecedented dataset. This article describes the PS1 Photometric Ladder, Release 12.01 This is the first of a series of data releases to be generated as the survey coverage increases and the data analysis improves. The Photometric Ladder has rungs every hour in RA and at 4 intervals in declination. We will release updates with increased area coverage (more rungs) from the latest dataset until the PS1 survey and the final re-reduction are completed. The currently released catalog presents photometry of $\sim 1000$ objects per square degree in the rungs of the ladder. Saturation occurs at $g_{\rm P1}, r_{\rm P1}, i_{\rm P1} \sim 13.5; z_{\rm P1} \sim 13.0;$ and $y_{\rm P1} \sim 12.0$. Photometry is provided for stars down to $g_{\rm P1}, r_{\rm P1}, i_{\rm P1} \sim 19.1$ in the AB system. This data release depends on the rigid `Ubercal' photometric calibration using only the photometric nights, with systematic uncertainties of (8.0, 7.0, 9.0, 10.7, 12.4) millimags in $(g_{\rm P1},r_{\rm P1},i_{\rm P1},z_{\rm P1},y_{\rm P1})$. Areas covered only with lower quality nights are also included, and have been tied to the Ubercal solution via relative photometry; photometric accuracy of the non-photometric regions is lower and should be used with caution.Physic

Cyclophosphamide- metabolizing enzyme polymorphisms and survival outcomes after adjuvant chemotherapy for node-positive breast cancer: a retrospective cohort study

Abstract Introduction Cyclophosphamide-based adjuvant chemotherapy is a mainstay of treatment for women with node-positive breast cancer, but is not universally effective in preventing recurrence. Pharmacogenetic variability in drug metabolism is one possible mechanism of treatment failure. We hypothesize that functional single nucleotide polymorphisms (SNPs) in drug metabolizing enzymes (DMEs) that activate (CYPs) or metabolize (GSTs) cyclophosphamide account for some of the observed variability in disease outcomes. Methods We performed a retrospective cohort study of 350 women enrolled in a multicenter, randomized, adjuvant breast cancer chemotherapy trial (ECOG-2190/INT-0121). Subjects in this trial received standard-dose cyclophosphamide, doxorubicin and fluorouracil (CAF), followed by either observation or high-dose cyclophosphamide and thiotepa with stem cell rescue. We used bone marrow stem cell-derived genomic DNA from archival specimens to genotype CYP2B6, CYP2C9, CYP2D6, CYP3A4, CYP3A5, GSTM1, GSTT1, and GSTP1. Cox regression models were computed to determine associations between genotypes (individually or in combination) and disease-free survival (DFS) or overall survival (OS), adjusting for confounding clinical variables. Results In the full multivariable analysis, women with at least one CYP3A4 *1B variant allele had significantly worse DFS than those who were wild-type *1A/*1A (multivariate hazard ratio 2.79; 95% CI 1.52, 5.14). CYP2D6 genotype did not impact this association among patients with estrogen receptor (ER) -positive tumors scheduled to receive tamoxifen. Conclusions These data support the hypothesis that genetic variability in cyclophosphamide metabolism independently impacts outcome from adjuvant chemotherapy for breast cancer

Substituting carbohydrate at lunch for added protein increases fat oxidation during subsequent exercise in healthy males

Context How pre-exercise meal composition influences metabolic and health responses to exercise later in the day is currently unclear. Objective Examine the effects of substituting carbohydrate for protein at lunch on subsequent exercise metabolism, appetite, and energy intake. Methods Twelve healthy males completed three trials in randomized, counterbalanced order. Following a standardized breakfast (779 ± 66 kcal; ∼08:15), participants consumed a lunch (1186 ± 140 kcal; ∼13:15) containing either 0.2 g·kg-1 carbohydrate and ∼2 g·kg-1 protein (LO-CARB), 2 g·kg-1 carbohydrate and ∼0.4 g·kg-1 protein (HI-CARB), or fasted (FAST). Participants later cycled at ∼60% V̇O2peak for 1 h (∼16:15) and post-exercise ad-libitum energy intake was measured (∼18:30). Substrate oxidation, subjective appetite, and plasma concentrations of glucose, insulin, non-esterified fatty acids (NEFA), peptide YY (PYY), glucagon-like peptide-1 (GLP-1), and acylated ghrelin (AG) were measured for 5 h post-lunch. Results Fat oxidation was greater during FAST (+11.66 ± 6.63 g) and LO-CARB (+8.00 ± 3.83 g) than HI-CARB (p < 0.001), with FAST greater than LO-CARB (+3.67 ± 5.07 g; p < 0.05). NEFA were lowest in HI-CARB and highest in FAST, with insulin demonstrating the inverse response (all p < 0.01). PYY and GLP-1 demonstrated a stepwise pattern, with LO-CARB greatest and FAST lowest (all p < 0.01). AG was lower during HI-CARB and LO-CARB versus FAST (p < 0.01). Energy intake in LO-CARB was lower than FAST (-383 ± 233 kcal; p < 0.001) and HI-CARB (-313 ± 284 kcal; p < 0.001). Conclusion Substituting carbohydrate for protein in a pre-exercise lunch increased fat oxidation, suppressed subjective and hormonal appetite, and reduced post-exercise energy intake