Expresión de ICAM-1 en el Endotelio de Arterias Humanas Mediante Inmunohistoquímica

Moore-Carrasco, R (reprint author), Univ Talca, Fac Ciencias Salud, Dept Bioquim Clin & Inmunohematol, Programa Invest Factores Riesgo Enfermedades Card, POB 747, Talca, Chile.Cardiovascular diseases (CVD) are the leading cause of death in the world. Among them the ischemic type are of great importance. where the development of atherosclerotic plaques is the central pathophysiological process. The study of atherosclerosis is critical to understand how this disease process begins and factors influencing its development. Various laboratory methods, including immunohistochemistry, allow the recognition of cells and molecules involved in the atheromatous process that are interacting according to the progression of the lesion. A marker of endothelial dysfunction is the increased expression of intercellular adhesion molecule ICAM-1. In this paper, an immunohistochemistry method was standardized for the adhesion molecule ICAM-1, and its expression was studied in healthy human arteries with atheromatous plaque. In samples of human arteries with atherosclerotic disease, the expression of ICAM-1 was observed lobe increased, but was hardly recognizable. This mainly because the tissue used as a control for standardization was a tonsil with an inflammatory process and hyperplasia, which significantly increases the expression of ICAM-1. The implementation of the immunohistochemistry method for ICAM-1 in human arteries will reveal endothelial dysfunction states that will enable a future design and implementation of methods of diagnosis in atherosclerotic processes in the early stages

Hole Hopping through Tryptophan in Cytochrome P450

Electron-transfer kinetics have been measured in four conjugates of cytochrome P450 with surface-bound Ru-photosensitizers. The conjugates are constructed with enzymes from Bacillus megaterium (CYP102A1) and Sulfolobus acidocaldarius (CYP119). A W96 residue lies in the path between Ru and the heme in CYP102A1, whereas H76 is present at the analogous location in CYP119. Two additional conjugates have been prepared with (CYP102A1)W96H and (CYP119)H76W mutant enzymes. Heme oxidation by photochemically generated Ru^(3+) leads to P450 compound II formation when a tryptophan residue is in the path between Ru and the heme; no heme oxidation is observed when histidine occupies this position. The data indicate that heme oxidation proceeds via two-step tunneling through a tryptophan radical intermediate. In contrast, heme reduction by photochemically generated Ru+ proceeds in a single electron tunneling step with closely similar rate constants for all four conjugates

Energy Spectrum Evolution of a Diffuse Field in Elastic Body Caused by Weak Nonlinearity

We study the evolution of diffuse elastodynamic spectral energy density under the influence of weak nonlinearity. It is shown that the rate of change of this quantity is given by a convolution of the linear energy at two frequencies. Quantitative estimates are given for sample aluminum and fused silica blocks of experimental interest.Comment: 9 pages, 3 figures; revised for better presentatio

Determining the Shape, Size, and Sources of the Zodiacal Dust Cloud using Polarized Ultraviolet Scattered Sunlight

The solar system's Zodiacal Cloud is visible to the unaided eye, yet the origin of its constituent dust particles is not well understood, with a wide range of proposed divisions between sources in the asteroid belt and Jupiter Family comets. The amount of dust contributed by Oort Cloud comets is uncertain. Knowledge of the Zodiacal Cloud's structure and origins would help with NASA's aim of characterizing potentially Earth-like planets around nearby stars, since the exo-Earths must be studied against the light scattered from extrasolar analogs of our cloud. As the only example where the parent bodies can be tracked, our own cloud is critical for learning how planetary system architecture governs the interplanetary dust's distribution. Our cloud has been relatively little-studied in the near-ultraviolet, a wavelength range that is important for identifying potentially-habitable planets since it contains the broad Hartley absorption band of ozone. We show through radiative transfer modeling that our cloud's shape and size at near-UV wavelengths can be measured from Earth orbit by mapping the zodiacal light's flux and linear polarization across the sky. We quantify how well the cloud's geometric and optical properties can be retrieved from a set of simulated disk observations, using a Markov chain Monte Carlo analysis. The results demonstrate that observations with sufficient precision, covering a set of fields distributed along the ecliptic and up to the poles, can be used to determine the division between asteroidal, Jupiter Family, and Oort Cloud dust components, primarily via their differing orbital inclination distributions. We find that the observations must be repeated over a time span of several months in order to disentangle the zodiacal light from the Galactic background using the Milky Way's rotation across the sky.Comment: submitted to PAS

ESI, a new Keck Observatory echellette spectrograph and imager

The Echellette Spectrograph and Imager (ESI) is a multipurpose instrument which has been delivered by the Instrument Development Laboratory of Lick Observatory for use at the Cassegrain focus of the Keck II telescope. ESI saw first light on August 29, 1999. ESI is a multi-mode instrument that enables the observer to seamlessly switch between three modes during an observation. The three modes of ESI are: An R=13,000-echellette mode; Low-dispersion prismatic mode; Direct imaging mode. ESI contains a unique flexure compensation system which reduces the small instrument flexure to negligible proportions. Long-exposure images on the sky show FWHM spot diameters of 34 microns (0.34") averaged over the entire field of view. These are the best non-AO images taken in the visible at Keck Observatory to date. Maximum efficiencies are measured to be 28% for the echellette mode and greater than 41% for low-dispersion prismatic mode including atmospheric, telescope and detector losses. In this paper we describe the instrument and its development. We also discuss the performance-testing and some observational results.Comment: 10 pages, 14 figures, 8tables, accepted for publication in PASP, 15 April 200

Evidence for a hopping mechanism in metal|single molecule|metal junctions involving conjugated metal–terpyridyl complexes; potential-dependent conductances of complexes [M(pyterpy)₂] ²⁺ (M = Co and Fe; pyterpy = 4′-(pyridin-4-yl)-2,2′:6′,2′′-terpyridine) in ionic liquid

Extensive studies of various families of conjugated molecules in metal|molecule|metal junctions suggest that the mechanism of conductance is usually tunnelling for molecular lengths < ca. 4 nm, and that for longer molecules, coherence is lost as a hopping element becomes more significant. In this work we present evidence that, for a family of conjugated, redox-active metal complexes, hopping may be a significant factor for even the shortest molecule studied (ca. 1 nm between contact atoms). The length dependence of conductance for two series of such complexes which differ essentially in the number of conjugated 1,4-C₆H₄- rings in the structures has been studied, and it is found that the junction conductances vary linearly with molecular length, consistent with a hopping mechanism, whereas there is significant deviation from linearity in plots of log(conductance) vs. length that would be characteristic of tunnelling, and the slopes of the log(conductance)–length plots are much smaller than expected for an oligophenyl system. Moreover, the conductances of molecular junctions involving the redox–active molecules, [M(pyterpy)₂] ²⁺/³⁺ (M = Co, Fe) have been studied as a function of electrochemical potential in ionic liquid electrolyte, and the conductance–overpotential relationship is found to fit well with the Kuznetsov–Ulstrup relationship, which is essentially a hopping description