The Role of Open Knowledge in Regional Development – Case Study

The paper describes the experience of CSP, a regional agency in the field of the Information Society. As catalyst for innovation in Piedmont region (north-west of Italy), CSP has been working in line with the regional strategy for the development of a knowledge-based economy. The paper outlines the key elements of the current strategy for regional development and describes the model and results implemented by CSP

La fortuna delle Genealogiae deorum gentilium nel ’500 italiano: da Marsilio Ficino a Giorgio Vasari

Negli ultimi decenni si sono moltiplicati gli studi critici sulla mitografia di Boccaccio e la sua fortuna. Accanto a ricerche più puntuali sulla fortuna di un determinato mito da Boccaccio al Rinascimento, il tema che maggiormente attira l’attenzione resta la difesa della poesia, che occupa i libri XIV e XV, forse i più celebri delle Genealogiae, un tema chiave anche nel seguire la sua fortuna rinascimentale, se si tiene conto dell’eco notevole presso gli intellettuali del ’500 e in particol..

On-sight and red-point climbing: changes in performance and route-finding ability in male advanced climbers

Aim: In lead climbing, the ascent of the route can be defined as on-sight or red-point. On-sight is the more challenging style since it demands greater physiological and psychological commitment. The differences between the two modes in advanced climbers have not been studied much. Two essential skills needed to optimize performance, in both on-sight and in red-point climbing, are route interpretation (RI) ability and movements sequence recall. Therefore, this study aimed to compare performance between on-sight and red-point ascent in advanced climbers and evaluate how a climber’s RI ability and movement sequences recall might change before and after on-sight and red-point climbing. Methods: Eighteen advanced male climbers (age 29.2 ± 4.7 years, body mass 67.8 ± 3.6 kg, stature 175.2 ± 2.4 cm, best red-point and on-sight grades 7b+/8a and 7a+/7b+, respectively) were video-recorded during the route ascent in on-sight and red-point modes to evaluate performance and to measure static and dynamic action times. RI ability and movement sequence recall were assessed before and after each climb. Level of anxiety was evaluated via a self-report questionnaire. Heart rate (fH), lactate concentration, ([La–]), and rating of perceived exertion (RPE) were detected during and after each climb. Results: Compared to on-sight, an improvement in performance was observed in a red-point climb: the ascent was faster (148.7 ± 13.6 s and 179.5 ± 12.5 s, respectively, P < 0.05), smoother (significant reduction in exploratory moves and in stops times, P < 0.05), less demanding physiologically (lower fHpeak and [La–]peak, P < 0.05), and psychologically (lower RPE, cognitive and somatic anxiety and higher self-confidence, P < 0.05). The RI ability was improved in red-point versus on-sight and, in the same mode, between pre and post ascent. Conclusion: Red-point climbing was found to be less demanding than on-sight, both physiologically and psychologically, under the conditions investigated by this study. Our findings suggest that RI is a trainable skill and underscore the importance of including specific techniques in training programs designed to improve interaction between perceptual, psychological, and physiological factors

Relationship between retinal microvascular impairment and subclinical atherosclerosis in SLE

objectives: patients with SLE have higher cardiovascular (CV) risk compared with healthy controls (HC) and are characterised by accelerated atherosclerosis; intima media thickness (IMT), marker of subclinical atherosclerosis, is higher in patients with SLE than in HCs. Retinal microvascular impairment detected through optical coherence tomography angiography (OCTA) was investigated as a marker of systemic vascular involvement in SLE.the aim of the study was to evaluate the relationship between retinal vascular impairment and IMT in SLE. methods: cross-sectional study recruiting patients with SLE and HCs. Data of the study population were collected. CV risk was evaluated through the american college of cardiology/american heart association (ACC/AHA) guidelines, framingham and QRESEARCH risk estimator V.3 (QRISK3) scores. Both groups underwent OCTA and carotid ultrasound with IMT assessment.Statistical analysis was accomplished using Pearson/Spearman, t-test/Mann-Whitney or χ2 test. Variables statistically significant at univariate regression analysis were tested in an age-corrected and sex-corrected multivariate regression model. results: 43 patients with SLE and 34 HCs were recruited.patients with SLE showed higher triglycerides (p=0.019), triglycerides-glucose (TyG) Index (p=0.035), ACC/AHA guidelines (p=0.001), Framingham Risk Scores (p=0.008) and a reduced superficial (p<0.001) and deep (p=0.005) whole retinal vessel density (VD) compared with HCs.In SLE univariate analysis, deep whole VD showed a negative correlation with IMT (p=0.027), age (p=0.001), systolic blood pressure (p=0.011), QRISK3 Score (p<0.001), systemic lupus international collaborating clinics damage index (p=0.006) and apolipoprotein B (p=0.021), while a positive correlation was found with female sex (p=0.029). Age-adjusted and sex-adjusted multivariate analysis confirmed QRISK3 score (p=0.049) and IMT (p=0.039) to be independent risk factors for reduced retinal VD. conclusions: patients with SLE showed lower retinal VD and higher CV risk indicators compared with HCs. Among patients with SLE, QRISK3 Score and IMT were found to be independent risk factors for retinal vascular impairment, suggesting a role of OCTA in evaluating preclinical CV involvement in SLE. moreover, TyG index could represent a biomarker of CV risk in patients with SLE compared with HCs

Cardiorespiratory and Metabolic Responses to Sinusoidal Exercise of Moderate Intensity: Reliability of the Measurements and the Effects of Fatigue

The cardiorespiratory and metabolic responses to sinusoidal exercise, in which work rate follows an oscillating pattern, have been proposed to assess the effectiveness of the cardiorespiratory adjustments. The repetition of successive sinusoidal periods permits to simultaneously reduce the influence of random fluctuations and accentuate the underlying physiological response. Data analysis has been often performed by overlapping and averaging successive cycles assuming no differences among them, thus excluding the possible presence of fatigue throughout successive cycles. After assessing the reliability of the measurements during sinusoidal exercise, this study sought to investigate the possible differences among subsequent cycles of sinusoidal work. Eleven active volunteers (age: 28±6 yrs., body mass: 73±7 kg; stature: 1.79±0.06 m, maximum oxygen uptake (VO2max): 52 ml·kg−1·min−1) participated to the study that was conducted in accordance with the Basic Principles of the Declaration of Helsinki. After determining individual VO2max and critical power (CP) on a cycle ergometer, they underwent sinusoidal work rates characterized by an amplitude (A), a midpoint (MP) and a period equal to ±50W, 50W below CP and 240s, respectively, up to exhaustion. On a different day, participants repeated the same experimental session for reliability purposes. Expiratory ventilation (VE), oxygen uptake (VO2), carbon dioxide output (VCO2), and heart rate (fH) responses were fitted by the sinewave function that minimized the residuals. A, MP and the time-delay (tD, the latency between mechanical work rate and physiological responses) of all parameters were determined for each cycle. Reliability assessment between day 1 and 2 was expressed as Cronbach’s a and intraclass correlation coefficient (ICC). A one-way ANOVA for repeated measures tested the presence of differences among cycles. Regression analysis was also applied to explore possible relationship between each variables and time. Reliability analysis revealed a very high to high ICC values for most of the parameters, with the exception of A for VO2 and VCO2 and tD for fH (moderate reliability). A of VE and fH response increased and decreased with time, respectively (p<0.05). MP of VE and fH showed a positive regression that led to significantly higher values in the last compared to the first cycle; on the contrary, no changes were observed among cycles in all other MP data. tD was similar in each cycle for all the investigated parameters despite a very slight negative regression found for VCO2. In conclusion, most of the physiological responses to moderate sinusoidal exercise exhibited a high to very high reliability. Some of the cardiorespiratory parameters showed significant changes with time throughout the sinusoidal exercise possibly due to the onset of fatigue. Therefore, an approach that overlaps and averages all the cycles together should not be performed to avoid wrong estimation of physiological responses to sinusoidal exercise, unless the averaging approach involves only the first cycles

PAK6 phosphorylates 14-3-3γ to regulate steady state phosphorylation of LRRK2

Mutations in Leucine-rich repeat kinase 2 (LRRK2) are associated with Parkinson's disease (PD) and, as such, LRRK2 is considered a promising therapeutic target for age-related neurodegeneration. Although the cellular functions of LRRK2 in health and disease are incompletely understood, robust evidence indicates that PD-associated mutations alter LRRK2 kinase and GTPase activities with consequent deregulation of the downstream signaling pathways. We have previously demonstrated that one LRRK2 binding partner is P21 (RAC1) Activated Kinase 6 (PAK6). Here, we interrogate the PAK6 interactome and find that PAK6 binds a subset of 14-3-3 proteins in a kinase dependent manner. Furthermore, PAK6 efficiently phosphorylates 14-3-3γ at Ser59 and this phosphorylation serves as a switch to dissociate the chaperone from client proteins including LRRK2, a well-established 14-3-3 binding partner. We found that 14-3-3γ phosphorylated by PAK6 is no longer competent to bind LRRK2 at phospho-Ser935, causing LRRK2 dephosphorylation. To address whether these interactions are relevant in a neuronal context, we demonstrate that a constitutively active form of PAK6 rescues the G2019S LRRK2-associated neurite shortening through phosphorylation of 14-3-3γ. Our results identify PAK6 as the kinase for 14-3-3γ and reveal a novel regulatory mechanism of 14-3-3/LRRK2 complex in the brain

Multiplicities of charged pions and unidentified charged hadrons from deep-inelastic scattering of muons off an isoscalar target

Multiplicities of charged pions and unidentified hadrons produced in deep-inelastic scattering were measured in bins of the Bjorken scaling variable $x$, the relative virtual-photon energy $y$ and the relative hadron energy $z$. Data were obtained by the COMPASS Collaboration using a 160 GeV muon beam and an isoscalar target ($^6$LiD). They cover the kinematic domain in the photon virtuality $Q^2$ > 1(GeV/c$)^2$, $0.004 < x < 0.4$, $0.2 < z < 0.85$ and $0.1 < y < 0.7$. In addition, a leading-order pQCD analysis was performed using the pion multiplicity results to extract quark fragmentation functions