Ensuring the Stability of the Genome: DNA Damage Checkpoints

The cellular response to DNA damage is vital for the cell�s ability to maintain genomic integrity. Checkpoint signalling pathways, which induce a cell cycle arrest in response to DNA damage, are an essential component of this process. This is reflected by the functional conservation of these pathways in all eukaryotes from yeast to mammalian cells. This review will examine the cellular response to DNA damage throughout the cell cycle. A key component of the DNA damage response is checkpoint signalling, which monitors the state of the genome prior to DNA replication (G1/S) and chromosome segregation (G2/M). Checkpoint signalling in model systems including mice, Xenopus laevis, Drosophila melanogaster, and the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe have been useful in elucidating these pathways in mammalian cells. An examination of this research, with emphasis on the function of checkpoint proteins, their relationship to DNA repair, and their involvement in oncogenesis is undertaken here

International Migration in Ireland, 2015

This working paper is the Irish report to the OECD Expert Group on Migration. As such, the focus of the report is largely shaped by the reporting requirements for the preparation of the annual OECD International Migration Outlook. The purpose of the paper is to outline major developments and trends in migration and integration data and policy. The principal reference year is 2014, although information relating to early- 2015 is included where available and relevant. The Executive Summary provides an overview of the main findings of the report. Section 2 discusses the main developments in migration and integration policy in Ireland in 2014, including topics related to migration in the public debate. Section 3 discusses the statistics on inward and outward migration movements. Section 4 examines trends in the population. Migration and the labour market are discussed in Section 5.Department of Justice and Equalit

The W100 pocket on HIV-1 gp120 penetrated by b12 is not a target for other CD4bs monoclonal antibodies

<p>Abstract</p> <p>Background</p> <p>The conserved CD4 binding site (CD4bs) on HIV-1 gp120 is a major target for vaccines. It is a priority to determine sites and structures within the CD4bs that are important for inclusion in vaccines. We studied a gp120 pocket penetrated by W100 of the potent CD4bs monoclonal antibody (mab), b12. We compared HIV-1 envelopes and corresponding mutants that carried blocked W100 pockets to evaluate whether other CD4bs mabs target this site.</p> <p>Findings</p> <p>All CD4bs mabs tested blocked soluble CD4 binding to gp120 consistent with their designation as CD4bs directed antibodies. All CD4bs mabs tested neutralized pseudovirions carrying NL4.3 wild type (wt) envelope. However, only b12 failed to neutralize pseudoviruses carrying mutant envelopes with a blocked W100 pocket. In addition, for CD4bs mabs that neutralized pseudovirions carrying primary envelopes, mutation of the W100 pocket had little or no effect on neutralization sensitivity.</p> <p>Conclusions</p> <p>Our data indicate that the b12 W100 pocket on gp120 is infrequently targeted by CD4bs mabs. This site is therefore not a priority for preservation in vaccines aiming to elicit antibodies targeting the CD4bs.</p

Cervicitis as a Clinical Indicator of Gonococcal and Chlamydial Infections in Pregnancy

Objective: We undertook the present study to attempt to apply clinical indicators predictive of cervical infection in nongravid populations with either Neisseria gonorrhoeae or Chlamydia trachomatis to our pregnant population and to determine the significance of the clinical diagnosis of “cervicitis.

UIT Detection of Hot Stars in the Globular Cluster NGC362

We used the Ultraviolet Imaging Telescope during the March 1995 Astro-2 mission to obtain a deep far-UV image of the globular cluster NGC 362, which was formerly thought to have an almost entirely red horizontal branch (HB). 84 hot (T_eff > 8500 K) stars were detected within a radius of 8'.25 of the cluster center. Of these, 43 have FUV magnitudes consistent with HB stars in NGC 362, and at least 34 are cluster members. The number of cluster members is made uncertain by background contamination from blue stars in the Small Magellanic Cloud (SMC). There are six candidate supra-HB stars which have probably evolved from the HB. We discuss the implications of these results for the production of hot blue stars in stellar populations.Comment: 10 pages AASLaTeX including one postscript figure and one compressed bitmap, .jpg format. To appear in Ap. J. Letters. Postscript version also available at http://www.astro.virginia.edu/~bd4r

Ultraviolet Imaging Observations of the cD Galaxy in Abell 1795: Further Evidence for Massive Star Formation in a Cooling Flow

We present images from the Ultraviolet Imaging Telescope of the Abell 1795 cluster of galaxies. We compare the cD galaxy morphology and photometry of these data with those from existing archival and published data. The addition of a far--UV color helps us to construct and test star formation model scenarios for the sources of UV emission. Models of star formation with rates in the range \sim5-20M_{\sun}yr$^{-1}$ indicate that the best fitting models are those with continuous star formation or a recent ($\sim4$ Myr old) burst superimposed on an old population. The presence of dust in the galaxy, dramatically revealed by HST images complicates the interpretation of UV data. However, we find that the broad--band UV/optical colors of this cD galaxy can be reasonably matched by models using a Galactic form for the extinction law with $E_{B-V}=0.14$. We also briefly discuss other objects in the large UIT field of view.Comment: To appear in the Astrophysical Journal. 14 AAS preprint style pages plus 7 figure

GALEX UV Color Relations for Nearby Early-Type Galaxies

We use GALEX/optical photometry to construct color-color relationships for early-type galaxies sorted by morphological type. We have matched objects in the GALEX GR1 public release and the first IR1.1 internal release, with the RC3 early-type galaxies having a morphological type -5.5<T<-1.5 with mean error in T<1.5, and mean error on (B-V)T<0.05. After visual inspection of each match, we are left with 130 galaxies with a reliable GALEX pipeline photometry in the far-UV and near-UV bands. This sample is divided into Ellipticals (-5.5<T<-3.5) and Lenticulars (-3.5<T<-1.5). After correction for the Galactic extinction, the color-color diagrams FUV-NUV vs. (B-V)_{Tc} are plotted for the two subsamples. We find a tight anti-correlation between the FUV-NUV and (B-V)_{Tc} colors for Ellipticals, the UV color getting bluer when the (B-V)_{Tc} get redder. This relationship very likely is an extension of the color-metallicity relationship into the GALEX NUV band. We suspect that the main source of the correlation is metal line blanketing in the NUV band. The FUV-NUV vs B-V correlation has larger scatter for lenticular galaxies; we speculate this reflects the presence of low level star formation. If the latter objects (i.e. those that are blue both in FUV-NUV and B-V) are interpreted as harboring recent star formation activity, this would be the case for a few percent (~4%) of Ellipticals and ~15% of Lenticulars; this would make about 10% of early-type galaxies with residual star formation in our full sample of 130 early-type galaxies. We also plot FUV-NUV vs. the Mg_2 index and central velocity dispersion. We find a tight anti-correlation between FUV-NUV and the Mg_2 index(...).Comment: 25 pages, 5 figures, accepted for publication in ApJS (abstract abridged), typos corrected in section 2.

Ultraviolet Imaging of the z=0.23 Cluster Abell 2246

We present deep ultraviolet observations of a field containing the cluster Abell 2246 (z=0.225) which provide far-ultraviolet (FUV) images of some of the faintest galaxies yet observed in that bandpass. Abell 2246 lies within the field of view of Ultraviolet Imaging Telescope (UIT) observations of the quasar HS1700+64, which accumulated over 7100 seconds of UIT FUV exposure time during the Astro-2 mission in March 1995. For objects found on both the FUV and ground-based V-band images, we obtain FUV (l ~ 1520 A) photometry and V-band photometry, as well as mid-UV (l ~ 2490 A) photometry from UIT Astro-1 observations and ground-based I-band photometry. We find five objects in the images which are probably galaxies at the distance of Abell 2246, with FUV magnitudes (m(FUV)) between 18.6 and 19.6, and V magnitudes between 18.4 and 19.6. We find that their absolute FUV fluxes and colors imply strongly that they are luminous galaxies with significant current star formation, as well as some relatively recent, but not current, (> 400 Myr ago) star formation. We interpret the colors of these five objects by comparing them with local objects, redshift-corrected template spectra and stellar population models, finding that they are plausibly matched by 10-Gyr-old population models with decaying star formation, with decay time constants in the range 3 Gyr < t < 5 Gyr, with an additional color component from a single burst of moderate ( ~ 400-500 Myr) age. From derived FUV luminosities we compute current star formation rates. We compare the UV properties of Abell 2246 with those of the Coma cluster, finding that Abell 2246 has significantly more recent star formation, consistent with the Butcher-Oemler phenomenon.Comment: Accepted for publication in the Astronomical Journal, June 1998. 17 Pages AAS latex, includes 4 bitmap .jpg format images and 4 other figures. PDF, Embedded Gzipped PS version (1.9Mb) TeX source and figures available at http://www.astro.virginia.edu/~bd4r/galaxies.htm