Backward recall and benchmark effects of working memory

Working memory was designed to explain four benchmark memory effects: the word length effect, the irrelevant speech effect, the acoustic confusion effect, and the concurrent articulation effect. However, almost all research thus far has used tests that emphasize forward recall. In four experiments, we examine whether each effect is observable when the items are recalled in reverse order. Subjects did not know which recall direction would be required until the time of test, ensuring that encoding processes would be identical for both recall directions. Contrary to predictions of both the primacy model and the feature model, the benchmark memory effect was either absent or greatly attenuated with backward recall, despite being present with forward recall. Direction of recall had no effect on the more difficult conditions (e.g., long words, similar-sounding items, items presented with irrelevant speech, and items studied with concurrent articulation). Several factors not considered by the primacy and feature models are noted, and a possible explanation within the framework of the SIMPLE model is briefly presented

Linking the literacy curriculum with technology

Evaluating the process and needs of linking literacy and technology in the educational setting. The technology is presented as the driving force of change in the literacy. Teachers, administrators, and parents need to formulate a plan for training sessions along with organizing supportive people around them to integrate technology into the curriculum. They need to design professional development to empower teachers to use technology, in the curriculum. Understanding that students are exposed to multimedia literacy, technology will be a medium to motivate and synthesize learning. Teachers facilitate students to be responsible and empowered learners for their own learning. There is evidence that technology can be integrated and linked to literacy and will work in all areas of the curriculum

From Brown-Peterson to continual distractor via operation span: A SIMPLE account of complex span

Three memory tasks—Brown-Peterson, complex span, and continual distractor—all alternate presentation of a to-be-remembered item and a distractor activity, but each task is associated with a different memory system, short-term memory, working memory, and long-term memory, respectively. SIMPLE, a relative local distinctiveness model, has previously been fit to data from both the Brown-Peterson and continual distractor tasks; here we use the same version of the model to fit data from a complex span task. Despite the many differences between the tasks, including unpredictable list length, SIMPLE fit the data well. Because SIMPLE posits a single memory system, these results constitute yet another demonstration that performance on tasks originally thought to tap different memory systems can be explained without invoking multiple memory systems

Increased susceptibility to proactive interference in adults with dyslexia?

Recent findings show that people with dyslexia have an impairment in serial-order memory. Based on these findings, the present study aimed to test the hypothesis that people with dyslexia have difficulties dealing with proactive interference (PI) in recognition memory. A group of 25 adults with dyslexia and a group of matched controls were subjected to a 2-back recognition task, which required participants to indicate whether an item (mis)matched the item that had been presented 2 trials before. PI was elicited using lure trials in which the item matched the item in the 3-back position instead of the targeted 2-back position. Our results demonstrate that the introduction of lure trials affected 2-back recognition performance more severely in the dyslexic group than in the control group, suggesting greater difficulty in resisting PI in dyslexia.Peer reviewedFinal Accepted Versio

Global Salmonella control in an integrated swine production system

Salmonellosis is a threat to the whole pork production chain. Different risks factors have been associated with Salmonella contamination. The aim of this project was to assess the efficacy of a comprehensive control program in a 30 000 sows integrated company located in Canada, with an emphasis on pre-harvest control measures, to limit Salmonella pork carcasses contamination. Based on previous molecular epidemiology investigations, the main sources of contamination were identified as the environment and the replacement gilts. The features of this 10 years program consisted of : Sourcing with negative replacement gilts, use of mash coarse feed on gilt finishers and sow herds, application of detailed cleaning and disinfection procedures on all farms, transport vehicles, slaughtering facilities and accessories, respect of all-in all-out and single source

Reanalysis of P2X(7) receptor expression in rodent brain

P2X receptors are cationic-selective ion channels gated by extracellular ATP. There are seven subunits (P2X1-7), the first six of which are expressed throughout the peripheral and central nervous systems. P2X7 receptors are rapidly upregulated and activated as a result of inflammatory stimuli in immune cells, where they act not only as cationic channels but uniquely couple with rapid release of proinflammatory cytokines, cytoskeletal rearrangements, and apoptosis or necrotic cell death. The P2X7 receptor has been termed the cytolytic non-neuronal P2X receptor because it had not been detected in neurons until recently when it has been immunolocalized to several brain regions, particularly the hippocampus, and has been suggested to be involved in presynaptic modulation of transmitter release. Because its expression in brain neurons may have substantial functional implications, we have performed detailed immunocytochemical, immunoblot, and immunoprecipitation studies on brain and non-neuronal tissue using all currently available antibodies. We first examined rats, but staining patterns were inconsistent among antibodies; we therefore studied mice for which there are two P2X7 knock-out mice constructs available, one expressing the LacZ transgene. We found that P2X7 receptor protein is strongly and reliably detected in the submandibular gland and lung of wild-type mice but not in either of the P2X7-/- mice. However, we failed to find evidence for P2X7 receptor protein in hippocampal neurons or their input-output projections. Either the P2X7 protein in the hippocampus is below the limits of detection by the currently available methods or it is not present

Dynamics of macrophage polarization reveal new mechanism to inhibit IL-1β release through pyrophosphates

In acute inflammation, extracellular ATP activates P2X7 ion channel receptors (P2X7R) on M1 polarized macrophages to release pro-inflammatory IL-1β through activation of the caspase-1/nucleotide-binding domain and leucine-rich repeat receptor containing pyrin domain 3 (NLRP3) inflammasome. In contrast, M2 polarized macrophages are critical to the resolution of inflammation but neither actions of P2X7R on these macrophages nor mechanisms by which macrophages switch from pro-inflammatory to anti-inflammatory phenotypes are known. Here, we investigated extracellular ATP signalling over a dynamic macrophage polarity gradient from M1 through M2 phenotypes. In macrophages polarized towards, but not at, M2 phenotype, in which intracellular IL-1β remains high and the inflammasome is intact, P2X7R activation selectively uncouples to the NLRP3-inflammasome activation but not to upstream ion channel activation. In these intermediate M1/M2 polarized macrophages, extracellular ATP now acts through its pyrophosphate chains, independently of other purine receptors, to inhibit IL-1β release by other stimuli through two independent mechanisms: inhibition of ROS production and trapping of the inflammasome complex through intracellular clustering of actin filaments

Activation kinetics of single P2X receptors

After the primary structure of P2X receptors had been identified, their function had to be characterized on the molecular level. Since these ligand-gated ion channels become activated very quickly after binding of ATP, methods with adequate time resolution have to be applied to investigate the early events induced by the agonist. Single-channel recordings were performed to describe conformational changes on P2X2, P2X4, and P2X7 receptors induced by ATP and also by allosteric receptor modifiers. The main results of these studies and the models of P2X receptor kinetics derived from these observations are reviewed here. The investigation of purinoceptors by means of the patch clamp technique following site-directed mutagenesis will probably reveal more details of P2X receptor function at the molecular level