Baconguis

Beharry

Bhargava

Brake

Browne

Evans

Fountain

Gonzales

Gorostiza

Hattori

J. A. Sim

J. P. M. Nunes

Jasti

Jiang

Kawate

Khakh

Kleinlogel

Krishtal

L. Bragg

L. E. Browne

Lewis

Lima

Matute

Nakazawa

North

Pettersen

R. Alan North

Rassendren

S. Caddick

Sali

Surprenant

Tochitsky

V. Chudasama

Valera

Volgraf

Werner

Woolley

English

Significance
          P2X receptors are trimeric proteins in which an extracellular transmitter (ATP) binds to open a transmembrane ion permeation pathway. We have designed a thiol-reactive, light-sensitive azobenzene molecule that can attach between cysteines introduced by mutagenesis into different channel subunits. Such chemically modified channels can be opened within milliseconds by irradiation at 440 nm, and closed by light at 360 nm, in the absence of any extracellular ligand. A similar result was obtained for heterotrimeric P2X2/3 receptors and for structurally unrelated (but trimeric) acid-sensing ion channels. The work extends our understanding of the molecular mechanism of gating and provides a tool to investigate the role of P2X receptors and acid-sensing ion channels in intact cells and tissues.</jats:p

Liam E. Browne

João P. M. Nunes

Joan A. Sim

Vijay Chudasama

Laricia Bragg

Stephen Caddick

Crossref

Proceedings of the National Academy of Sciences

Optical control of trimeric P2X receptors and acid-sensing ion channels

http://www.pnas.org/content/111/1/521.full.pdf

Optical control of trimeric P2X receptors and acid-sensing ion channels

Abstract

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