Aloe Vera Functionalized Magnetic Nanoparticles Entrapped Ca Alginate Beads as Novel Adsorbents for Cu(II) Removal from Aqueous Solutions

UIDP/5006/2020. Publisher Copyright: © 2022 by the authors.CABs (Ca alginate beads), AVCABs (Aloe vera Ca alginate beads), and AVMNCABs (Aloe-vera functionalized magnetic nanoparticles entrapped Ca alginate beads) were developed as adsorbents for the removal of Cu(II) from aqueous solutions. The materials were characterized using Fourier-transform infrared (FTIR) spectroscopy, high-resolution scanning electron microscopic (HR-SEM) analysis, X-ray diffraction (XRD), energy-dispersive X-ray (EDX) spectroscopy, and a vibrating-sample magnetometer (VSM). The effect of several parameters, such as pH, time, temperature, adsorbent dose, etc., were investigated. The adsorption isotherm of Cu(II) was adjusted best to the Langmuir model. The maximum adsorption capacities were 111.11 mg/g, 41.66 mg/g, and 15.38 mg/g for AVMNCABs, AVCABs, and CABs, respectively. The study of the adsorption kinetics for Cu(II) ions on beads followed a pseudo-second-order kinetic model, with a very good correlation in all cases. The adsorption studies used a spectrophotometric method, dealing with the reaction of Cu(II) with KSCN and variamine blue.publishersversionpublishe

Calcium alginate beads with entrapped iron oxide magnetic nanoparticles functionalized with methionine—a versatile adsorbent for arsenic removal

no. 3114/8/Fin./Sch.// 2018 UIDP/50006/2020A novel beads adsorbent, consisting of calcium alginate entrapped on magnetic nanoparti-cles functionalized with methionine (MFMNABs), was developed for effective elimination of arsenic from water. The material was characterized by FT-IR (Fourier Transform Infrared Spectroscopy), SEM (Scanning Electron Microscopic), XRD (X-ray Diffraction) and TEM (Transmission Electron Microscopy). The arsenic removal capacity of the material was studied by altering variables such as pH of the solution, contact time, adsorbent dose and adsorbate concentration. The maximal removal of As(III) was 99.56% under optimal conditions with an equilibrium time of 110 min and pH 7.0–7.5. The adsorption followed a second order kinetics and data best fitted the Langmuir isotherm with a correlation coefficient of R2 = 0.9890 and adsorption capacity (qm ) of 6.6533 mg/g. The thermodynamic study showed entropy change (∆S) and enthalpy change (∆H) to be 34.32 J mol−1 K and 5.25 kJ mol−1, respectively. This study proved that it was feasible to treat an As(III) solution with MFMNABs. The synthesized adsorbent was cost-effective, environmentally friendly and versatile, compared to other adsorbents. The adsorption study was carried by low cost spectrophotometric method using N-bromosuccinimide and rhodamine-B developed in our laboratory.publishersversionpublishe

<b>Microwave assisted spectrophotometric method for the determination of copper using leucocrystal violet</b>

A sensitive spectrophotometric method has been developed for the trace determination of copper using leucocrystal violet as chromogenic reagent. The proposed method is based on the reaction of copper(II) with potassium iodide in acid medium to liberate iodine, which oxidizes leucocrystal violet to crystal violet dye having absorption maximum at 590 nm. The reaction between copper and potassium iodide is accelerated by irradiating the mixture with microwave energy for 15 s at 480 W. Beer’s law is obeyed in the concentration range 0.004 – 0.04 &mu;g mL^-1. The molar absorptivity, Sandell’s sensitivity, detection limit and quantitation limit were found to be 1.47 x 10⁶ L mol^-1 cm^-1, 4.3 x 10^-5 &mu;g mL^-2, 0.001 &mu;g mL^-1, 0.0043 &mu;g mL^-1, respectively. The optimum conditions and other analytical parameters were evaluated. The effect of interfering ions on the determination is described. The proposed method has been successfully applied for the determination of copper in environmental samples like tap water, agricultural run off water, plant materials and pharmaceutical samples

Genetic epidemiology of autoinflammatory disease variants in Indian population from 1029 whole genomes

Abstract Background Autoinflammatory disorders are the group of inherited inflammatory disorders caused due to the genetic defect in the genes that regulates innate immune systems. These have been clinically characterized based on the duration and occurrence of unprovoked fever, skin rash, and patient’s ancestry. There are several autoinflammatory disorders that are found to be prevalent in a specific population and whose disease genetic epidemiology within the population has been well understood. However, India has a limited number of genetic studies reported for autoinflammatory disorders till date. The whole genome sequencing and analysis of 1029 Indian individuals performed under the IndiGen project persuaded us to perform the genetic epidemiology of the autoinflammatory disorders in India. Results We have systematically annotated the genetic variants of 56 genes implicated in autoinflammatory disorder. These genetic variants were reclassified into five categories (i.e., pathogenic, likely pathogenic, benign, likely benign, and variant of uncertain significance (VUS)) according to the American College of Medical Genetics and Association of Molecular pathology (ACMG-AMP) guidelines. Our analysis revealed 20 pathogenic and likely pathogenic variants with significant differences in the allele frequency compared with the global population. We also found six causal founder variants in the IndiGen dataset belonging to different ancestry. We have performed haplotype prediction analysis for founder mutations haplotype that reveals the admixture of the South Asian population with other populations. The cumulative carrier frequency of the autoinflammatory disorder in India was found to be 3.5% which is much higher than reported. Conclusion With such frequency in the Indian population, there is a great need for awareness among clinicians as well as the general public regarding the autoinflammatory disorder. To the best of our knowledge, this is the first and most comprehensive population scale genetic epidemiological study being reported from India