Naiset Ylen Teksti-TV:n käyttäjinä

Tämä opinnäytetyö käsittelee Ylen Teksti-TV:n naiskäyttäjiä. Tutkielman päätarkoituksena on selvittää, kuinka usein naiset käyttävät Ylen tekstitelevisiota, mitä tietoa he sieltä hakevat ja mitä tietoja he kaipaisivat sinne lisää. Tarkoituksena on myös selvittää, voisiko julkisen palvelun Yleisradio palvella naispuolista väestöä entistä paremmin ja monipuolisemmin tekstitelevision avulla. Vastaukset tutkimuskysymyksiin saadaan käyttäjäkyselyn avulla, joka toteutettiin Webropol 2.0. -ohjelmalla kahden viikon aikana helmi−maaliskuun vaihteessa vuonna 2012. Tutkimuskysely on samalla tilaustyö Ylelle. Opinnäytetyössä käsitellään yleisesti, millainen rooli tekstitelevisiolla on ollut vuosien varrella Suomessa, millainen rooli sillä tänä päivänä on ja miltä teksti-tv:n tulevaisuus näyttää. Tutkielmassa paneudutaan nimenomaan Ylen Teksti-TV:hen ja sen 30-vuotiseen historiaan. Työssä luodaan myös katsaus maamme muihin tekstitelevisioihin ja ulkomaiden tilanteeseen. Teksti-tv-osuuden jälkeen tutkielmassa käsitellään naisia median käyttäjinä. Opinnäytetyössä katsastetaan naisten roolia ja asemaa median käyttäjinä sekä kuluttajina. Tutkielmassa tehdään selkoa myös Yleisradion toimintatavoitteista julkisen palvelun yhtiönä. Tutkielmaa varten on käyty läpi kirjallisuutta ja aikakausilehtiä sekä muutamia opinnäytetöitä. Näiden lisäksi opinnäytetyötä varten on haastateltu asiantuntijoita, jotka ovat olleet tekemisissä teksti-tv:n kanssa. Käyttäjäkyselystä käy ilmi, että teksti-tv on internetin aikakaudellakin edelleen tärkeä osa ihmisten elämää. Kyselystä käy ilmi, että keskimääräinen Ylen Teksti-TV:tä käyttävä nainen asuu Uudellamaalla ja on iältään 35–49-vuotias. Ylen Teksti-TV:tä käytetään ensisijaisesti uutisten sekä urheilutulosten katsomiseen. Tämän tutkimuskyselyn perusteella näiden tietojen saamista teksti-tv:stä pidetään helppona. Valtaosa kyselyyn vastaajista on myös varsin tyytyväisiä Ylen Teksti-TV:n tämänhetkiseen tilaan. Vastaajat antoivat palvelulle arvosanaksi 4,04 viiden ollessa paras.This bachelor’s thesis converses on women as users in Finland's national public broadcasting company Yle´s text-tv channel. The object of the thesis work is to clarify how often women use Yle’s text-tv channel, what text-tv information is searched for and what information is additionally desired by women. Our main objective is to study whether the public broadcasting company Yleisradio is capable of meeting the needs of feminine population in greater lengths and more diversely through its text-tv service. The answers to our problem setting were summoned by a user inquiry which was executed with a software, Webropol 2.0. The user inquiry is simultaneously a customization for Yle. The thesis in general presents what kind of role text-tv has played in Finland during the years, what its role is today and what the future has in store for text-tv in Finland. The thesis concentrates especially on Yle’s text-tv channel and its three-decade history. The customization carries out an overview about the other Finnish text-tv services and text-tv services popularity abroad. After the basic text-tv part the thesis focuses on women as media users. Notably the thesis also reveals the role and status of women in media field. The thesis explores and explains Yleisradio’s aspirations as a national public-broadcasting company. The material for the thesis consists of printed works, numerous magazines since the early days of text-tv in Finland and some other published thesis's on text-tv. Furthermore some text-tv experts have been interviewed for this thesis. The user inquiry results show that text-tv is despite the Internet era an integral part of people’s lives. The inquiry reveals also that an average woman using Yle’s text-tv lives in Uusimaa region and is 35–49 years old. Firstly Yle's text-tv channel is used for checking news headlines and sports results. According to the inquiry the easy usability is the main reason why Yle text-tv channel is still in use in the 2010s. The majority of answerers are quite satisfied with the state of Yle’s text-tv services today. The users graded the service with a 4,04 rating, five being the maximum

The Digital Anatomist Information System and Its Use in the Generation and Delivery of Web-Based Anatomy Atlases

Advances in network and imaging technology, coupled with the availability of 3-D datasets such as the Visible Human, provide a unique opportunity for developing information systems in anatomy that can deliver relevant knowledge directly to the clinician, researcher or educator. A software framework is described for developing such a system within a distributed architecture that includes spatial and symbolic anatomy information resources, Web and custom servers, and authoring and end-user client programs. The authoring tools have been used to create 3-D atlases of the brain, knee and thorax that are used both locally and throughout the world. For the one and a half year period from June 1995–January 1997, the on-line atlases were accessed by over 33,000 sites from 94 countries, with an average of over 4000 ‘‘hits’’ per day, and 25,000 hits per day during peak exam periods. The atlases have been linked to by over 500 sites, and have received at least six unsolicited awards by outside rating institutions. The flexibility of the software framework has allowed the information system to evolve with advances in technology and representation methods. Possible new features include knowledge-based image retrieval and tutoring, dynamic generation of 3-D scenes, and eventually, real-time virtual reality navigation through the body. Such features, when coupled with other on-line biomedical information resources, should lead to interesting new ways for managing and accessing structural information in medicine

Knowledge-Based Client-Server Approach to Structural Information Retrieval: The Digital Anatomist Browser

Structural information can be defined as data and knowledge about biological objects ranging in size from molecules to the whole body. A framework is described for organizing structural information around a well-defined set of terminology and semantic relationships, and for disseminated multimedia structural information by means of a wide-area information server that is accessible over the internet. A Macintosh-based client of this server, called the Digital Anatomist Browser, has been used to teach neuroanatomy for the last 2 years. The client-server approach provides each student unlimited access to a rapidly growing knowledge base of structral biology that, while immediately useful for anatomy teaching, has the potential to be an organizing framework for other kinds of medial knowledge as well

Etablering på den svenska marknaden

Detta examensarbete undersöker etablering på den svenska marknaden med fokus på företaget TK Engineering Oy som är specialiserade inom CAN-teknologi. De planerar nu att börja erbjuda konsulttjänster på den svenska marknaden och behöver därför en plan för genomförandet. Den svenska marknaden är attraktiv för finska företag på grund av dess närhet och starka affärsklimat, men det finns förstås utmaningar som kräver anpassning till lokal affärskultur och konkurrensförhållanden. Syftet var att identifiera möjligheter och utmaningar för TK Engineering vid en expansion till Sverige samt utforma grunden till en strategisk marknadsplan som förslagsvis kunde stödja deras tillväxt. Undersökningen genomfördes med kvalitativ forskningsmetod i form av intervjuer med företagets redan befintliga partners på svenska marknaden samt TK Engineerings personal. Resultaten visar på att spetskunskap och referensbaserade nätverk är centrala för att lyckas som ett konsultföretag. Word-of-mouth marknadsföring, deltagande i branschspecifika mässor samt aktiv närvaro på digitala plattformar identifierades som effektiva strategier. Dessutom motiverades kulturell förståelse och lokala samarbeten för att stärka marknadsnärvaron. TK Engineering behöver utveckla långsiktiga partnerskap och förstärka sin differentiering genom att erbjuda unika och högkvalitativa lösningar som är anpassade till den svenska marknaden. Med rätt strategi och resurser har företaget goda förutsättningar att expander framgångsrikt och skapa långvariga kundrelationer i Sverige.Tämä opinnäytetyö tutkii Ruotsin markkinoille laajentumista, keskittyen TK Engineering Oy - yritykseen, joka on erikoistunut CAN-teknologiaan. Yritys suunnittelee konsultointipalveluiden tarjoamista Ruotsissa ja tarvitsee siksi toteutussuunnitelman. Ruotsin markkinat ovat houkuttelevat suomalaisille yrityksille niiden läheisyyden ja vahvan liiketoimintaympäristön vuoksi, mutta haasteita on, jotka edellyttävät sopeutumista paikalliseen yrityskulttuuriin ja kilpailuolosuhteisiin. Työn tavoitteena oli tunnistaa mahdollisuuksia ja haasteita, joita TK Engineering kohtaa laajentaessaan toimintaansa Ruotsiin, sekä luoda pohja strategiselle markkinointisuunnitelmalle, joka voisi tukea heidän kasvuaan. Tutkimus toteutettiin laadullisin tutkimusmenetelmin haastattelemalla yrityksen nykyisiä kumppaneita Ruotsin markkinoilla sekä TK Engineeringin henkilökuntaa. Tulokset osoittavat, että erikoisosaaminen ja viittausperusteiset verkostot ovat keskeisiä menestykselle konsultointialalla. Suosittelumarkkinointi, osallistuminen alakohtaisiin messuihin ja aktiivinen läsnäolo digitaalisilla alustoilla tunnistettiin tehokkaiksi strategioiksi. Lisäksi kulttuurinen ymmärrys ja paikalliset yhteistyökumppanuudet korostettiin tärkeinä markkina-aseman vahvistamisessa. TK Engineeringin tulee kehittää pitkäaikaisia kumppanuuksia ja vahvistaa erilaistumistaan tarjoamalla ainutlaatuisia ja korkealaatuisia ratkaisuja, jotka on räätälöity Ruotsin markkinoille. Oikealla strategialla ja resursseilla yrityksellä on hyvät edellytykset laajentua menestyksekkäästi ja luoda kestäviä asiakassuhteita Ruotsissa.This thesis examines market entry into the Swedish market, focusing on the company TK Engineering Oy, which specializes in CAN technology. The company plans to begin offering consulting services in Sweden and therefore requires an implementation plan. The Swedish market is attractive to Finnish companies due to its proximity and strong business climate, but challenges remain that demand adaptation to the local business culture and competitive conditions. The aim was to identify opportunities and challenges for TK Engineering in their expansion to Sweden and to develop the foundation for a strategic marketing plan that could potentially support their growth. The study was conducted using qualitative research methods, including interviews with the company’s existing partners in the Swedish market as well as TK Engineering's personnel. The results indicate that specialized expertise and reference-based networks are central to succeeding as a consulting firm. Word-of-mouth marketing, participation in industry-specific trade fairs, and an active presence on digital platforms were identified as effective strategies. Furthermore, cultural understanding and local partnerships were highlighted as crucial for strengthening market presence. TK Engineering needs to develop long-term partnerships and enhance their differentiation by offering unique and high-quality solutions tailored to the Swedish market. With the right strategy and resources, the company has good potential to expand successfully and establish lasting customer relationships in Sweden

The use of proteomics in identifying differentially expressed serum proteins in humans with type 2 diabetes

BACKGROUND: The aim of the study was to optimize protocols for finding and identifying serum proteins that are differentially expressed in persons with normal glucose tolerance (NGT) compared to individuals with type 2 diabetes mellitus (T2DM). Serum from persons with NGT and persons with T2DM was profiled using ProteinChip arrays and time-of-flight mass spectra were generated by surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). RESULTS: Mass spectra from NGT- and T2DM-groups were compared. Fifteen proteins ranging from 5 to 79 kDa were differentially expressed (p < 0.05). Five of these proteins showed decreased and ten showed increased serum levels in individuals with T2DM. To be able to identify the proteins, the complexity of the sample was reduced by fractionation approaches. Subsequently, the purified fractions containing biomarkers were separated by one-dimensional SDS-polyacrylamide gel electrophoresis (SDS-PAGE) in two identical lanes. Protein bands of the first lane were excised and subjected to passive elution to recapture the biomarkers on ProteinChip arrays. The corresponding bands of the second lane were subjected to peptide-mass fingerprinting (PMF). Using this approach four of the differentially expressed proteins were identified as apolipoprotein C3 (9.4 kDa), transthyretin (13.9 kDa), albumin (66 kDa) and transferrin (79 kDa). Whereas apolipoprotein C3 and transthyretin were up-regulated, albumin and transferrin were down-regulated in T2DM. CONCLUSION: Protocols for protein profiling by SELDI-TOF MS and protein identification by fractionation, SDS-PAGE and PMF were optimized for serum from humans with T2DM. With these protocols differentially expressed proteins were discovered and identified when serum from NGT- and T2DM-individuals was analyzed

Role of MAPK in apolipoprotein CIII-induced apoptosis in INS-1E cells

<p>Abstract</p> <p>Background</p> <p>Individuals with type 2 diabetes mellitus (T2DM) have elevated levels of circulating apolipoprotein CIII (apoCIII). ApoCIII plays an important role for plasma triglyceride levels and elevated levels of the apolipoprotein have been connected with dyslipidemia in T2DM subjects. In addition, apoCIII has been linked to enhanced β-cell apoptosis. The present study was undertaken to investigate apoptotic mechanisms induced by the apolipoprotein.</p> <p>Results</p> <p>ApoCIII (10 μg/ml) enhanced apoptosis 2-fold in insulin-producing INS-1E cells after 24 hours exposure to the apolipoprotein. At this time point phosphorylation of mitogen activated protein kinase (MAPK) p38 had doubled but ERK1/2 and JNK were not activated. Instead, ERK1/2 showed rapid and transient phosphorylation (2-fold after 0.5 hour). No JNK phosphorylation was observed. In support of a role of activation of not only p38 but also ERK1/2 in apoCIII-induced apoptosis, inclusion of p38 inhibitor SB203580 (10 μM) or ERK1/2 inhibitor PD98059 (100 μM) normalized apoptosis. Whereas influx of Ca²⁺was linked to apoCIII-induced ERK1/2 activation, pro-apoptotic protein CHOP/GADD of the unfolded protein response (UPR) was not affected by apoCIII.</p> <p>Conclusion</p> <p>It is suggested that elevated circulating apoCIII levels may contribute to β-cell apoptosis via activation of p38 and ERK1/2 in individuals with T2DM. Therapies aiming at normalizing levels of apoCIII could be beneficial not only for the function of the β-cell but also for cardiovascular protection.</p

Combining GPR and historical aerial photographs to investigate river channel morphodynamics, Oldman River, southern Alberta

River channels are dynamic landforms that play an important role in regulating riparian ecosystems. At a fundamental level, river erosion and deposition produce changes in the riparian zone that lead to the creation of new habitat or reworking of existing habitat. Despite considerable riparian research along the Oldman River in southern Alberta, little is known about historical channel dynamics and the resulting implications for riparian habitat. This research paper documents long term dynamics of a segment of the Oldman River at Fort Macleod, AB. Specifically, historical aerial photographs and a ground penetrating radar (GPR) were used to reconstruct how the river channel and bar features evolved over the last 70 years. Results from the aerial photographs indicate that the channel has undergone pronounced changes since 1938, with a trend of increasing meander curvature and decreasing wavelength. Channel bank reinforcement at Fort Macleod will likely constrict further meander development and force continued downstream meander migration. Subsurface imaging of a channel bar with GPR reveals a dominant mode of vertical accretion by bedload deposition at high flow stages, indicating a compound feature formed by overlapping unit bars. Observations of other bars along the Oldman River indicate similar modes of emplacement. Overall, results from this case study provide important context for assessing recent changes in riparian habitat. Application of this approach to other river segments will be of value in assessing whether similar patterns of channel and bar dynamics exist throughout the Oldman watershed

Neuronatin: A New Inflammation Gene Expressed on the Aortic Endothelium of Diabetic Mice

OBJECTIVE—Identification of arterial genes and pathways altered in obesity and diabetes

Localized-Statistical Quantification of Human Serum Proteome Associated with Type 2 Diabetes

BACKGROUND: Recent advances in proteomics have shed light to discover serum proteins or peptides as biomarkers for tracking the progression of diabetes as well as understanding molecular mechanisms of the disease. RESULTS: In this work, human serum of non-diabetic and diabetic cohorts was analyzed by proteomic approach. To analyze total 1377 high-confident serum-proteins, we developed a computing strategy called localized statistics of protein abundance distribution (LSPAD) to calculate a significant bias of a particular protein-abundance between these two cohorts. As a result, 68 proteins were found significantly over-represented in the diabetic serum (p<0.01). In addition, a pathway-associated analysis was developed to obtain the overall pathway bias associated with type 2 diabetes, from which the significant over-representation of complement system associated with type 2 diabetes was uncovered. Moreover, an up-stream activator of complement pathway, ficolin-3, was observed over-represented in the serum of type 2 diabetic patients, which was further validated with statistic significance (p = 0.012) with more clinical samples. CONCLUSIONS: The developed LSPAD approach is well fit for analyzing proteomic data derived from biological complex systems such as plasma proteome. With LSPAD, we disclosed the comprehensive distribution of the proteins associated with diabetes in different abundance levels and the involvement of ficolin-related complement activation in diabetes