6 research outputs found
Exposure to a Human Relevant Mixture of Persistent Organic Pollutants or to Perfluorooctane Sulfonic Acid Alone Dysregulates the Developing Cerebellum of Chicken Embryo
Acknowledgements This project has received funding from the European Unionâs Horizon 2020 research and innovation program under the Marie SkĆodowska-Curie grant agreement No. 722634 (http://protected.eu.com/). The authors gratefully acknowledge the Proteomics Core Facility of the University of Aberdeen for their support & assistance in this work. The sequencing service was provided by the Norwegian Sequencing Centre (www.sequencing.uio.no), a national technology platform hosted by the University of Oslo and supported by the "Functional Genomics" and "Infrastructure" programs of the Research Council of Norway and the South-eastern Regional Health Authorities.Peer reviewedPublisher PD
Contrasting transcriptome response to thermal stress in two key zooplankton species, Calanus finmarchicus and C. glacialis
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LSH and G9a/GLP complex are required for developmentally programmed DNA methylation
LSH, a member of the SNF2 family of chromatin remodeling ATPases encoded by the Hells gene, is essential for normal levels of DNA methylation in the mammalian genome. While the role of LSH in the methylation of repetitive DNA sequences is well characterized, its contribution to the regulation of DNA methylation and the expression of protein-coding genes has not been studied in detail. In this report we investigate genome-wide patterns of DNA methylation at gene promoters in Hellsâ/â mouse embryonic fibroblasts (MEFs). We find that in the absence of LSH, DNA methylation is lost or significantly reduced at âŒ20% of all normally methylated promoter sequences. As a consequence, a large number of genes are misexpressed in Hellsâ/â MEFs. Comparison of Hellsâ/â MEFs with wild-type MEFs and embryonic stem (ES) cells suggests that LSH is important for de novo DNA methylation events that accompany the establishment and differentiation of embryonic lineage cells. We further show that the generation of normal DNA methylation patterns and stable gene silencing at specific promoters require cooperation between LSH and the G9a/GLP complex of histone methylases. At such loci, G9a recruitment is compromised when LSH is absent or greatly reduced. Taken together, our data suggest a mechanism whereby LSH promotes binding of DNA methyltransferases and the G9a/GLP complex to specific loci and facilitates developmentally programmed DNA methylation and stable gene silencing during lineage commitment and differentiation
Multi-omics approach reveals dysregulated genes during hESCs neuronal differentiation exposure to paracetamol
Summary: Prenatal paracetamol exposure has been associated with neurodevelopmental outcomes in childhood. Pharmacoepigenetic studies show differences in cord blood DNA methylation between unexposed and paracetamol-exposed neonates, however, causality and impact of long-term prenatal paracetamol exposure on brain development remain unclear. Using a multi-omics approach, we investigated the effects of paracetamol on an in vitro model of early human neurodevelopment. We exposed human embryonic stem cells undergoing neuronal differentiation with paracetamol concentrations corresponding to maternal therapeutic doses. Single-cell RNA-seq and ATAC-seq integration identified paracetamol-induced chromatin opening changes linked to gene expression. Differentially methylated and/or expressed genes were involved in neurotransmission and cell fate determination trajectories. Some genes involved in neuronal injury and development-specific pathways, such as KCNE3, overlapped with differentially methylated genes previously identified in cord blood associated with prenatal paracetamol exposure. Our data suggest that paracetamol may play a causal role in impaired neurodevelopment