Development of a balloon-born Peltier-based chilled-mirror hygrometer for the troposphere and the lower stratosphere

We have developed a new balloon-borne hygrometer, which is based on a chilled-mirror principle and uses a two stage Peltier cooler. In January 2012, we have conducted flight tests at Biak, Indonesia (1.180S, 136.110E) to evaluate the performances of this sensor. The result showed that this hygrometer has the ability to measure atmospheric water vapor from the surface to the lower stratosphere. It is considered that our hygrometer is helpful for TTL observation campaigns because of its ease-in-handling

P2Y12 Receptor Blockade Augments Glycoprotein IIb‐IIIa Antagonist Inhibition of Platelet Activation, Aggregation, and Procoagulant Activity

Background: New antiplatelet agents that provide greater, more consistent inhibition of the platelet ADP receptor P2Y12 may be used in combination with glycoprotein (GP) IIb‐IIIa antagonists, but their combined effect on platelet function and procoagulant activity is not well studied. Therefore, the objective of this study was to evaluate the independent and complementary effects of P2Y12 and GPIIb‐IIIa inhibition on platelet function and procoagulant activity. Methods and Results: Healthy donor blood was treated with the active metabolite of prasugrel (R‐138727 5 μmol/L), GPIIb‐IIIa antagonists (abciximab 3 μg/mL or eptifibatide 0.9 μg/mL), and combinations thereof, exposed to physiologically relevant agonists (collagen and ADP) and then evaluated for markers of platelet activation and procoagulant activity. Significant interactions between R‐138727 and GPIIb‐IIIa antagonists were observed. R‐138727 and the GPIIb‐IIIa antagonists had additive inhibitory effects on collagen‐stimulated platelet aggregation and on the collagen plus ADP–stimulated level of activated platelet surface GPIIb‐IIIa. R‐138727 and abciximab each inhibited collagen plus ADP–stimulated platelet phosphatidylserine expression and prothrombin cleavage, and the combination produced greater inhibition than achieved with abciximab alone. In contrast, eptifibatide did not inhibit, but instead enhanced, collagen plus ADP–stimulated prothrombin cleavage. Addition of R‐138727 reduced prothrombin cleavage in eptifibatide‐treated samples, suggesting a novel mechanism for potential benefit from combined prasugrel and eptifibatide treatment. Conclusions: The complementary effects of abciximab and R‐138727 on platelet activation, aggregation, and procoagulant activity suggest their combined use may, to a greater degree than with either agent alone, reduce thrombus formation in vivo

Genetic variation of CYP2C19 affects both pharmacokinetic and pharmacodynamic responses to clopidogrel but not prasugrel in aspirin-treated patients with coronary artery disease

The metabolic pathways leading to the formation of prasugrel and clopidogrel active metabolites differ. We hypothesized that decreased CYP2C19 activity affects the pharmacokinetic and pharmacodynamic response to clopidogrel but not prasugrel. Ninety-eight patients with coronary artery disease (CAD) taking either clopidogrel 600 mg loading dose (LD)/75 mg maintenance dose (MD) or prasugrel 60 mg LD/10 mg MD were genotyped for variation in six CYP genes. Based on CYP genotype, patients were segregated into two groups: normal function (extensive) metabolizers (EM) and reduced function metabolizers (RM). Plasma active metabolite concentrations were measured at 30 min, 1, 2, 4, and 6 h post-LD and during the MD period on Day 2, Day 14, and Day 29 at 30 min, 1, 2, and 4 h. Vasodilator-stimulated phosphoprotein (VASP) and VerifyNow (TM) P2Y12 were measured predose, 2, and 24 +/- 4 h post-LD and predose during the MD period on Day 14 +/- 3 and Day 29 +/- 3. For clopidogrel, active metabolite exposure was significantly lower (P = 0.0015) and VASP platelet reactivity index (PRI, %) and VerifyNow (TM) P2Y(12) reaction unit (PRU) values were significantly higher (P < 0.05) in the CYP2C19 RM compared with the EM group. For prasugrel, there was no statistically significant difference in active metabolite exposure or pharmacodynamic response between CYP2C19 EM and RM. Variation in the other five genes demonstrated no statistically significant differences in pharmacokinetic or pharmacodynamic responses. Variation in the gene encoding CYP2C19 in patients with stable CAD contributes to reduced exposure to clopidogrel's active metabolite and a corresponding reduction in P2Y(12) inhibition, but has no significant influence on the response to prasugrel

Impact of a New Temperature-Dependence Correction on Historical Meisei Radiosonde Humidity Data

A new temperature-dependence correction (T-D correction) for Meisei RS2-91, RS-01G, and RS-06G radiosonde relative humidity (RH) measurements has been developed recently to remove the artificial stepwise change of ~3% RH at 0°C associated with the present (original) correction. These radiosondes have been used at most of the Japanese upper-air stations since the 1990s. The historical radiosonde humidity records at Sapporo and Tateno stations on the 925, 700, and 500 hPa pressure levels show apparent large downward trends between 1999 and 2009. This is because the original T-D correction has only been applied since February 2003 after a moist bias was discovered. The new T-D correction is found to result in a much smaller downward RH trend at Sapporo and almost no trend at Tateno

Correction of the Stepwise Change Observed at 0℃ in Meisei RS2-91, RS-01G, and RS-06G Radiosonde Relative Humidity Profiles

Comparisons of relative humidity (RH) measurements between the Meisei RS-06G radiosonde and a chilled-mirror hygrometer revealed that the RS-06G radiosonde shows a stepwise change of similar to 3% RH at 0℃ (drying when air temperature is decreasing). This is due to a discontinuous correction factor in the processing software that compensates for the temperature dependence of the RH sensor. Results from chamber experiments regarding the temperature and RH dependence of RS-06G RH sensors under steady-state conditions showed a wet bias exceeding 7% RH below similar to+10℃. As this result contradicted previous in-flight intercomparisons that used the original manufacturer's correction, we investigated a possible additional dry bias caused by a thermal lag in the RH sensor. We speculated that the thermal lag of the RH sensor typically causes a dry bias during a tropospheric ascent, which largely compensates for the wet bias related to the temperature and RH dependence of the RH sensor. We observed that the experimental results of the temperature and RH dependence considering the thermal lag were in agreement with the extrapolation of the original manufacturer's correction. Consequently, we proposed to extrapolate the original manufacturer's correction, which is currently applied at temperatures between -40℃ and 0℃, up to +14.5℃ to resolve the artificial stepwise change at 0℃. Because the RS-06G radiosonde is a successor to the Meisei RS-01G and RS2-91 radiosondes, which have adopted the same RH sensor material installed since July 1999 and have used the same processing software, the current results should be applied to the data obtained by those radiosondes. The bias of RS-06G RH measurements using this new correction is estimated to be within 7% RH, which is within the manufacturer's specifications, being drier at +40℃ and wetter between -40℃ and +10℃

Development of a New Particle Imaging Radiosonde with Particle Fall Velocity Measurements in Clouds

A new particle imaging radiosonde “Rainscope” has been developed, and for the first time, particle fall velocity measurement functionality was added to a balloon-borne device. Rainscope can capture a clear still image of precipitation particles in a cloud when they interrupt an infrared beam, using a CMOS camera equipped with an electronic shutter. It can also record the time when a particle passes the upper and lower built-in infrared sensors, enabling measurement of the velocity of falling precipitation particles. For ground testing in rain and snow, a ground-based Rainscope showed raindrop/snowflake size-fall velocity distributions similar to those obtained in previous studies. In a comparison with a Parsivel2 disdrometer in rain, the Rainscope results were in good agreement with the distributions obtained by an adjacent Parsivel2. In a test flight of Rainscope into a stratiform cloud, raindrops, mostly melted particles, snowflakes in the process of melting, graupel, and snowflakes were observed. It was observed that the fall velocity varied depending on the type of solid precipitation particles

Comparison of GRUAN data products for Meisei iMS-100 and Vaisala RS92 radiosondes at Tateno, Japan

A total of 99 dual soundings with Meisei iMS-100 radiosonde and Vaisala RS92 radiosondes were carried out at the Aerological Observatory of the Japan Meteorological Agency, known as Tateno (36.06∘ N, 140.13∘ E, 25.2 m; the World Meteorological Organization, WMO, station number 47646), from September 2017 to January 2020. Global Climate Observing System (GCOS) Reference Upper-Air Network (GRUAN) data products (GDPs) from both sets of radiosonde data for 59 flights were subsequently created using a documented processing programme along with the provision of optimal estimates for measurement uncertainty. Differences in radiosonde performance were then quantified using these GDPs. For daytime observations, the iMS-100 temperature is around 0.5 K cooler than RS92-GDP in the stratosphere, with significant differences in the upper troposphere and lower stratosphere in consideration of combined uncertainties. For nighttime observations, the difference is around −0.1 K, and data are mostly in agreement. For relative humidity (RH), iMS-100 is around 1 % RH–2 % RH higher in the troposphere and 1 % RH smaller in the stratosphere than RS92, but both GDPs are in agreement for most of the profile. The mean pressure difference is ≤0.1 hPa, the wind speed difference is from −0.04 to +0.14 m s−1, the wind direction difference is , and the root mean square vector difference (RMSVD) for wind is ≤1.04 m s−1