Hubungan antara pH Susu dengan Jumlah Sel Somatik Sebagai Parameter Mastitis Subklinik

The aim of this research was to measure the relationship of the pH value to the somatic cell count as a parameter of sub clinical mastitis detection. Two hundreds quarter milk samples were used in this research and the test (the pH value, IPB-1 mastitis test and Breed method) was done in parallel way. The results showed that 152 samples from 200 samples (76%) tested with Breed method came from the herds which suffered from sub-clinical mastitis and with IPB-1 test showed that 145 (72.5%) of the samples had positive reaction. Using pH meter, it showed that 44 samples (22 %) had pH > 6.75, presumed suffered from sub-clinical mastitis and 2 samples (1%) showed pH < 6.30 (6.25 and 6.28). At the same time, these two samples showed a negative reaction with IPB-1 test and had somatic cell count of 360,000/ml and 280,000/ml, each. It also showed that there was a close relationship between pH value and IPB-1 test. The conclusion of this research was that the measurement of pH value was not a sensitive method for detecting sub-clinical mastitis. Key words: sub-clinical mastitis, pH, somatic cell count, IPB-1 mastitis tes

Penambahan Boraks Dalam Bakso Dan Faktor Pendorong Penggunaannya Bagi Pedagang Bakso Di Kota Bengkulu

The research of using borax in meatballs which was undertaken in the Bengkulu City in 2011 showed that 10% from 100 samples of meatballs containing borax. Considering the dangers of borax for the consumer if consume continuously and increasing the number of meatball traders until 2013, it is necessary to study with more meatballs samples and widely area. The aim of the study was to detect the presence of borax in meatballs, borax content after heating, storability meatballs which containing borax and determine the factors that support the meatball traders to use borax. The study was designed using cross sectional study using two types of data, they were data of meatball sample test in the laboratory and interview data to meatball traders in the BengkuluCity. Total of 160 meatball samples from traders and five meatball samples from grinders were collected and examined with qualitative test using turmeric paper. The qualitative test showed that 165 samples (100%) takenfrom meatball traders and grinders didn't contain borax. Knowledge and attitudes level of traders were at a good level. There were significant relationship between education level with knowledge, information source fromtelevision with attitude, and knowledge with attitude of traders in Bengkulu City (p < 0,05)

Pengembangan Metode Promosi Kesehatan Tentang Rabies untuk Peningkatan Pengetahuan Siswa Sekolah Dasar

Kendala utama promosi kesehatan pada siswa sekolah dasar sebagai kelompok berisiko tinggi serangan rabies adalah terbatasnya media promosi kesehatan yang sesuai.Tujuan penelitian ini adalah mengembangkan media promosi kesehatan yang mudah, murah dan efektif. Penelitian ini dilakukan pada 90 siswa, kelas 5 dan 6 berasal dari 3 sekolah di Kota Bogor. Sampel dipilih dengan metode simple random sampling, dibagi menjadi 3 kelompok intervensi yang berjumlah 30 siswa untuk setiap kelompok yaitu kelompok I (slide, poster, leaflet, dan permainan), kelompok II (slide, poster, dan leaflet), dan kelompok III (slide). Rancangan uji coba menggunakan pretest-post test design. Pengukuran tingkat pengetahuan dilakukan sesaat, 1 minggu, 1 bulan, dan 3 bulan setelah intervensi.Analisis data dilakukan dengan uji t berpasangan dan Duncan multiple range test. Hasil uji coba terdapat perbedaan tingkat pengetahuan sebelum dan sesudah intervensi hingga 3 bulan pada kelompok I dan II (p&lt;0.05), sedangkan pada kelompok III hanya hingga 1 bulan (p&gt;0.05).Selisih pengetahuan sebelum dan sesudah intervensi antar 3 kelompok pada 3 bulan terdapat perbedaan yang nyata (P&lt;0.05).Kelompok I memberikan hasil tingkat pengetahuan paling tinggi dan daya ingat paling lama dibandingkan kelompok lainnya.Hasil penelitian ini dapat digunakan sebagai sarana promosi kesehatan mengenai rabies pada siswa sekolah dasar

Deteksi Spesies Brucella pada Kambing di Rumah Potong Hewan Jakarta

Brucellosis is a zoonosis and occupational diseases transmision. The diseases caused by bacterial and attack multiple species of animals. Common species that infects goats as the most pathogenic species (zoonotic) is Brucella melitensis; however, the species B. abortus could also infect goats. The study purposed to find out the brucellosis seropositive in goat in Jakarta slaughterhouse and to detect caused agent of brucellosis. Sampling was done through slaughtered goats that come from brucellosis endemic area. The samples were collected fromslaughtered mature female goats i.e serum, goat milk, vaginal swab, mamary gland, limphoglandula supramamary, limph, and uterus. The detection method was used i.e patological lession, serological, culture and PolymeraseChain Reaction (PCR) technique. The serological detection of brucellosis in goats was done parallelly between Rose Bengal Test (RBT), Complement Fixation Test (CFT) and Enzyme Linked Immunosorbent Assay (ELISA). The results of this study demonstrated that out of the 119 serum samples serologically tested, negative for RBT, one was positive for CFT and none were positive with ELISA. Patological observation in the Brucella predilection organs, there were 5 goat carcases showed pathological lession (vagina discharge, hemoragy at limphand limphoglandula, crumbly limph and there were pus in uterus). The serum samples that had reacted positively and the organs with pathological lesion were confirmed further with PCR, bacterial isolation and identification.The PCR test results and the culture of milk samples, vaginal swabs and organs did not reveal any Brucella spp bacteria (B. abortus, B. melitensis, B. ovis dan B. suis) and also vaccine strains of RB51. Based on these results, it was concluded that brucellosis in goats on Java Island was a 0.84% seropositive (confidence interval 95%; 0.00826 - 0.00854) (1/119), although the species of Brucella that had infected them remains unknown

Profil Peternakan Babi di Kota Kupang dan Potensi Penularan Trichinellosis

Trichinellosis is a parasitic disease of humans caused by eating raw from domestic or game animals infected by Trichinella spp. Human trichinellosis contracted from commercial supplies of meat have been most often linked to infected pigs, wild boar, or horses. Trichinella is a nematode which has an atypical direct life cycle that does not involve stages developing outside of the host. This study was conducted to see the profile of pig farms in the city of Kupang and the potential transmission of trichinellosis. The data was derived from interview 60 farmers in 6 sub districts in city of Kupang by using a structured questionnaire and analyzed descriptively. The results of the questionnaire survey showed that many race of pig from a mixed race, the seeds come from traditional breeding. Feed rest of the home or restaurant are usually directly given to the pigs. The presence of rat in around of the cage often. All respondents were interviewed did not know or hear about trichinellosis disease which can be one cause of the spread of trichinellosis in city of Kupang

Prevalence of Mcr-1 Colistin Resistance Gene in Escherichia Coli Along Broiler Meat Supply Chain in Indonesia

Colistin is the last drug choice for dealing with carbapenem-resistant Enterobacteriaceae; therefore, this drug is very crucial for human health. The discovery of a plasmid-mediated colistin resistance gene, mobilized colistin resistance-1 (mcr-1), signals a significant global health threat. Colistin sulfate is an antimicrobial agent which has been approved for use in broilers in Indonesia. The purposes of this study were to measure the prevalence of colistin resistant E. coli and to detect the mcr-1 colistin resistance gene in E. coli, and E. coli O157:H7 in the entire supply chain of broilers in Bogor Regency, West Java Province, Indonesia. Samples were taken from flocks that use colistin sulfate (cloacal swabs, drinking water, and litters), small-scale poultry slaughterhouses (fresh meats and plucker swabs), traditional markets (fresh meats), and small restaurants (cooked meats). Isolation of E. coli was done on each sample and 493 isolates were obtained. All E. coli isolates were then tested for their susceptibility to colistin sulfate by the agar dilution method. Detection of mcr-1 gene from colistin resistant isolates (minimum inhibitory concentration &gt; 2 µg/mL) was conducted using polymerase chain reaction. The prevalence of colistin resistant E. coli from all isolates was 11.76% (CI 95%; CL 9.21–14.91%), and the prevalence of mcr-1 gene was 10.55% (CI 95%; CL 8.13–13.57%). There was a very good agreement between colistin resistance phenotype and mcr-1 gene (ĸ = 0.939). The mcr-1 gene was found in 89.66% colistin resistant E. coli isolates. Two colistin resistance and mcr-1 carrying gene isolates were identified as E. coli O157:H7 serotype. This study was the first research on mcr-1 gene in Indonesia which covers the entire supply chain of broiler meat from farms to consumers. These results showed the necessity to emphasize a reduced use of colistin sulfate in broiler management and to improve biosecurity measure, not only in farms but also in the entire supply chain of broiler meat