R.S. Stricklin III, Composition

Musikalisches Opfer (2015); So come on, night (2017); Transience (2014); Birth and Death Within a Day (2016); Absolute Lithops Effect (2017

Using “Expert Employees” to identify Duties and Tasks for CADD Technicians in North Central Idaho: Lessons Learned from a Modified DACUM Process

Objective: We conducted a job analysis to identify the competency profile of an entry level CADD technician in North central Idaho. Background: In 2011, the Clearwater Economic Development Association and NIMA collaborated with Lewis-Clark State College and the University of Idaho in a project to strengthen the capacity of Career and Technology Education (CTE) teachers to teach computer aided drafting and designing (CADD). An application to the National Science Foundation, Advanced Technology Education (NSF ATE) program was made and funding began July of 2011. Method: A modified DACUM was used. A total of seven expert workers from six companies participated. Results: The DACUM research chart created consisted of 8 duty statements and 54 task statements. Conclusions: Effective facilitation helps to reduce self-serving biases. The panel of experts believed the modified DACUM expedited the development of a new DACUM Research Chart that is reflective of what is done in the region

A novel mechanism for the establishment of sister chromatid cohesion by the ECO1 acetyltransferase

Cohesin complex mediates cohesion between sister chromatids, which promotes high-fidelity chromosome segregation. Eco1p acetylates the cohesin subunit Smc3p during S phase to establish cohesion. The current model posits that this Eco1p-mediated acetylation promotes establishment by abrogating the ability of Wpl1p to destabilize cohesin binding to chromosomes. Here we present data from budding yeast that is incompatible with this Wpl1p-centric model. Two independent in vivo assays show that a wpl1∆ fails to suppress cohesion defects of eco1∆ cells. Moreover, a wpl1∆ also fails to suppress cohesion defects engendered by blocking just the essential Eco1p acetylation sites on Smc3p (K112, K113). Thus removing WPL1 inhibition is insufficient for generating cohesion without ECO1 activity. To elucidate how ECO1 promotes cohesion, we conducted a genetic screen and identified a cohesion activator mutation in the SMC3 head domain (D1189H). Smc3-D1189H partially restores cohesion in eco1∆ wpl1∆ or eco1 mutant cells but robustly restores cohesion in cells blocked for Smc3p K112 K113 acetylation. These data support two important conclusions. First, acetylation of the K112 K113 region by Eco1p promotes cohesion establishment by altering Smc3p head function independent of its ability to antagonize Wpl1p. Second, Eco1p targets other than Smc3p K112 K113 are necessary for efficient establishment.National Institutes of Health (U.S.) (Grant R01GM092813

Analysis of Alternative Storage Conditions for DNA Recovery from Field Samples

As ecologists increasingly employ molecular methods, they find that tried and true preservation solutions (e.g. ethanol or formalin) may not be optimal when samples are targeted for genetic analyses. Before traveling to remote sample sites, researchers need to consider which preservation methods are likely to yield the largest quantity and highest quality of DNA based on their travel times and field conditions. They also need to consider whether they will have access to preservatives at remote sites and whether those preservatives can be safely transported. To determine which preservation methods would most reliably preserve tissue for genetic analysis under a range of field conditions, we examined total DNA recovery from female fruit flies (Drosophila melanogaster) individually held in various solutions (70% ethanol; 2% SDS, 100 mM EDTA; 1% SDS, 50 mM EDTA; 0.66% SDS, 33 mM EDTA; Zymo© lysis buffer; Zymo Xpedition© lysis buffer) at three different temperatures (22oC, 4oC and -20oC) for varying lengths of time (1 day, 4 weeks, and 8 weeks). We predicted that insects held in Zymo Xpedition© buffer would yield the overall highest DNA recovery since this buffer was designed for field collected animal tissue. We also predicted that variation in DNA recovery from insects held in different solutions would increase with preservation time and holding temperature. Although we observed significant differences in total DNA recovery from some of our samples, no trends were identified. Preliminary band quality analyses of PCR products utilizing stored DNA as template for amplification of the mCOI gene generally indicated decline in product quality as storage time increased. Future work will focus on better quantifying stored DNA quality and examining the relationship between total DNA recovered and overall DNA quality

Do Small, Patchy, Constructed Intertidal Oyster Reefs Reduce Salt Marsh Erosion As Well As Natural Reefs?

One ecological service that oyster reefs provide is stabilization of shorelines through reduced wave energy and erosion from boat traffic, storms, and predominant wind direction. Additionally, increasing sedimentation can enhance the growth of emergent marsh vegetation which further stabilizes unconsolidated sediments. A 21 mo study of constructed (with only 30-35% coverage) and natural oyster reefs in 3 bayous in the Grand Bay National Estuarine Research Reserve (NERR) suggested constructed reefs benefit this retrograding deltaic ecosystem. The marsh edge adjacent to all constructed reefs was less eroded (mean = 0.043 m) than edges adjacent to natural reefs (mean = 0.728 m), although all natural and constructed sites, regardless of bayou, illustrated large variations in marsh edge growth. The marsh edge in constructed sites in one bayou retreated more than in the other bayous, most likely due to its coarser sediments, greater boat traffic, and its apparent higher energy location within the landscape. By the end of this study, the ecological function of constructed oyster reefs in all bayous, as measured by marsh edge erosion reduction, was equivalent or exceeded the function in nearby natural oyster reefs. The physical structure of the reef further served to reduce erosion and marsh loss and this approach may be useful for management of a retrograding deltaic estuarine ecosystem like the Grand Bay NERR

Sector Expansion and Elliptical Modeling of Blue-Gray Ovoids for Basal Cell Carcinoma Discrimination in Dermoscopy Images

Background: Blue-gray ovoids (B-GOs), a critical dermoscopic structure for basal cell carcinoma (BCC), offer an opportunity for automatic detection of BCC. Due to variation in size and color, B-GOs can be easily mistaken for similar structures in benign lesions. Analysis of these structures could afford accurate characterization and automatic recognition of B-GOs, furthering the goal of automatic BCC detection. This study utilizes a novel segmentation method to discriminate B-GOs from their benign mimics. Methods: Contact dermoscopy images of 68 confirmed BCCs with B-GOs were obtained. Another set of 131 contact dermoscopic images of benign lesions possessing B-GO mimics provided a benign competitive set. A total of 22 B-GO features were analyzed for all structures: 21 color features and one size feature. Regarding segmentation, this study utilized a novel sector-based, non-recursive segmentation method to expand the masks applied to the B-GOs and mimicking structures. Results: Logistic regression analysis determined that blue chromaticity was the best feature for discriminating true B-GOs in BCC from benign, mimicking structures. Discrimination of malignant structures was optimal when the final B-GO border was approximated by a best-fit ellipse. Using this optimal configuration, logistic regression analysis discriminated the expanded and fitted malignant structures from similar benign structures with a classification rate as high as 96.5%. Conclusions: Experimental results show that color features allow accurate expansion and localization of structures from seed areas. Modeling these structures as ellipses allows high discrimination of B-GOs in BCCs from similar structures in benign images

Co-Governance and Resilience Justice

This is the author accepted manuscript. Available from the Digital Library of the Commons, Indiana University Libraries via the link in this recordIn co-governance, governance authority and management responsibility for resources, environments, or infrastructure are shared by the government and communities. Distinct institutions of co-governance emerge and evolve from the grassroots struggles of marginalized and oppressed communities for justice, empowerment, and resilience (capacities to adapt to disturbances and changing conditions). Instead of merely forming commons or merely making government processes/structures more participatory and inclusive, these communities form new institutional structures that integrate governmental power and resources with community power and resources. We show that co-governance institutions arise less out of a concern for efficient and sustainable use of resources and more out of a concern for social equity and community capacities. Building on Ostrom’s work on polycentric collective governance institutions and more recent scholars’ studies of key features of co-governance, we articulate the design principles of co-governance institutions that are specifically aimed at equity (justice) and community resilience in marginalized and oppressed communities. Using examples of co-governance in both Global South and Global North regions, we explore how these design principles address resilience justice problems. Workshop on the Ostrom Workshop – WOW7 Conference Indiana University, Bloomington, IN June 19-21, 202

Sensitive sexualities: dichotomized discourse in the erasure of bisexuality

A combination of Q methodology and a Think Aloud task explored how cultural knowledge about bisexuality is constructed and maintained. Q methodology revealed positive interpretations of bisexuality. Critical Discourse Analysis of the Think Aloud task however, exposed the maintenance of dualistic categories of sex, gender and sexuality acting as ‘operating systems’ and strategically guiding the social representation of bisexuality as ‘non-existent’, ‘deviant’, ‘abnormal’ and/or ‘promiscuous’. The findings of this study suggest that overt heterosexism is not becoming extinct; instead it has found rather subtle ways of incorporating itself into ‘liberal’ discourses

Locomotion disorders and skin and claw lesions in gestating sows housed in dynamic versus static groups

Lameness and lesions to the skin and claws of sows in group housing are commonly occurring indicators of reduced welfare. Typically, these problems are more common in group housing than in individual housing systems. Group management type (dynamic versus static) and stage of gestation influence the behavior of the animals, which in turn influences the occurrence of these problems. The present study compared prevalence, incidence and mean scores of lameness and skin and claw lesions in static versus dynamic group housed sows at different stages of gestation during three consecutive reproductive cycles. A total of 10 Belgian sow herds were monitored; 5 in which dynamic groups and 5 in which static groups were utilized. All sows were visually assessed for lameness and skin lesions three times per cycle and the claws of the hind limbs were assessed once per cycle. Lameness and claw lesions were assessed using visual analogue scales. Static groups, in comparison with dynamic groups, demonstrated lower lameness scores (P<0.05) and decreased skin lesion prevalence (24.9 vs. 47.3%, P<0.05) at the end of gestation. There was no difference between treatment group regarding claw lesion prevalence with 75.5% of sows demonstrating claw lesions regardless of group management. Prevalences of lameness (22.4 vs. 8.9%, P<0.05) and skin lesions (46.6 vs. 4.4%, P<0.05) were highest during the group-housed phase compared to the individually housed phases. Although the prevalence of lameness and skin lesions did not differ three days after grouping versus at the end of the group-housing phase, their incidence peaked during the first three days after moving from the insemination stalls to the group. In conclusion, the first three days after grouping was the most risky period for lameness incidence, but there was no significant difference between static or dynamic group management