37 research outputs found
La revuelta de las mujeres : Un análisis de "Mooladè" de Ousmane Sembene
Me aproximaré a la película "Mooladè" de Ousmane Sembene desde una serie de conceptos e ideas. En atención a la naturaleza dialógica del mundo social (Voloshinov; 1992) en primer lugar me remitiré a la “teoría de las voces” (Bidaseca et. al 2009), tratando de identificar en el dialogo social descrito por la película aquellas voces altas y bajas y en que contexto cada una asume estas intensidades, pero no solo esto; creo que la película en si misma puede ser considerada también una voz a través de la que hablan sus creadores y las instituciones que la apoyan. Por ello brevemente describiré y cada tanto me remitiré al contexto africano en el que esta voz pretende insertarse ya que trata una temática que involucra fuertemente a ese continente. Para esto me serviré, brevemente de los aportes de Quijano (2003) y Wallerstein (1979) para entender la inserción de Africa en el “sistema mundo”.Facultad de Humanidades y Ciencias de la Educació
Intradermal DNA Electroporation Induces Cellular and Humoral Immune Response and Confers Protection against HER2/neu Tumor
Skin represents an attractive target for DNA vaccine delivery because of its natural richness in APCs, whose targeting may
potentiate the effect of vaccination. Nevertheless, intramuscular electroporation is the most common delivery method for ECTM
vaccination. In this study we assessed whether intradermal administration could deliver the vaccine into different cell types and
we analyzed the evolution of tissue infiltrate elicited by the vaccination protocol. Intradermal electroporation (EP) vaccination
resulted in transfection of different skin layers, as well as mononuclear cells. Additionally, we observed a marked recruitment of
reactive infiltrates mainly 6–24 hours after treatment and inflammatory cells included CD11c+. Moreover, we tested the efficacy
of intradermal vaccination against Her2/neu antigen in cellular and humoral response induction and consequent protection from
a Her2/neu tumor challenge in Her2/neu nontolerant and tolerant mice. A significant delay in transplantable tumor onset was
observed in both BALB/c ( ≤ 0,0003) and BALB-neuT mice ( = 0,003). Moreover, BALB-neuT mice displayed slow tumor
growth as compared to control group ( < 0,0016). In addition, while in vivo cytotoxic response was observed only in BALB/c
mice, a significant antibody response was achieved in both mouse models. Our results identify intradermal EP vaccination as a
promising method for delivering Her2/neu DNA vaccine
MKK3 sustains cell proliferation and survival through p38DELTA MAPK activation in colorectal cancer
: Colorectal cancer (CRC) is one of the most common malignant tumors worldwide and understanding its underlying molecular mechanisms is crucial for the development of therapeutic strategies. The mitogen-activated protein kinase-kinase 3 (MKK3) is a specific activator of p38 MAP kinases (p38 MAPKs), which contributes to the regulation of several cellular functions, such as proliferation, differentiation, apoptosis as well as response to drugs. At present, the exact MKK3/p38 MAPK pathway contribution in cancer is heavily debated because of its pleiotropic function. In this work, we retrospectively explored the prognostic and pathobiologic relevance of MKK3 in a cohort of CRC patients and assessed MKK3 molecular functions in a panel of CRC lines and colonocytes primary cultures. We found increased MKK3 levels in late-stage CRC patients which correlated with shorter overall survival. Herein, we report that the MKK3 targeting by inducible RNA interference univocally exerts antitumor effects in CRC lines but not in primary colonocytes. While MKK3 depletion per se affects growth and survival by induction of sustained autophagy and death in some CRC lines, it potentiates response to chemotherapeutic drug 5-fluorouracil (5-FU) in all of the tested CRC lines in vitro. Here, we demonstrate for the first time that in CRC the MKK3 specifically activates p38delta MAPK isoform to sustain prosurvival signaling and that such effect is exacerbated upon 5-FU challenge. Indeed, p38delta MAPK silencing recapitulates MKK3 depletion effects in CRC cells in vitro and in vivo. Overall, our data identified a molecular mechanism through which MKK3 supports proliferation and survival signaling in CRC, further supporting MKK3 as a novel and extremely attractive therapeutic target for the development of promising strategies for the management of CRC patients
Identification of Relevant Conformational Epitopes on the HER2 Oncoprotein by Using Large Fragment Phage Display (LFPD)
We developed a new phage-display based approach, the Large Fragment Phage Display (LFPD), that can be used for
mapping conformational epitopes on target molecules of immunological interest. LFPD uses a simplified and more effective
phage-display approach in which only a limited set of larger fragments (about 100 aa in length) are expressed on the phage
surface. Using the human HER2 oncoprotein as a target, we identified novel B-cell conformational epitopes. The same
homologous epitopes were also detected in rat HER2 and all corresponded to the epitopes predicted by computational
analysis (PEPITO software), showing that LFPD gives reproducible and accurate results. Interestingly, these newly identified
HER2 epitopes seem to be crucial for an effective immune response against HER2-overexpressing breast cancers and might
help discriminating between metastatic breast cancer and early breast cancer patients. Overall, the results obtained in this
study demonstrated the utility of LFPD and its potential application to the detection of conformational epitopes on many
other molecules of interest, as well as, the development of new and potentially more effective B-cell conformational
epitopes based vaccines
The use of renal biopsy in the kidney tumor management: A retrospective analysis of consecutive cases in a referral center
Introduction: Ultrasound-guided renal masses biopsy (RMB) is a useful and underestimated tool to evaluate suspected renal tumors. This study aimed to assess the safety and feasibility of this technique. Materials and Methods: Data of 80 patients with suspected primary or secondary kidney tumors who underwent RMB between January 2012 and December 2020 were included in this retrospective study. Twelve patients were excluded due to incomplete data. Biopsy outcomes were collected through our electronic medical records system and then compared with definitive pathology. Results: RMB was performed in 68 cases. Pathological examination reported 43 (63%) malignant cases, while RMB was negative in 15 (22%) samples. On the other hand, a benign lesion was present in 8 (12%) cases, and 2 (3%) biopsies were non diagnostic. One major and one minor post-procedure complication were reported among the patients. A total of 31 patients underwent renal surgery including 19 partial and 12 radical nephrectomies. Out of them, 4 patients had a negative biopsy, but radiological imaging strongly suggested malignancy. The concordance between biopsy and definitive pathology occurred in 22 out of 31 (71%) cases, with a higher rate among the masses greater than 4 cm, 9/11 (82%) compared to smaller ones 13/20 (65%). Pathologic examination of the 4 cases with negative biopsy showed 3 renal cell and a translocation renal cell carcinoma. Conclusions: Ultrasound-guided biopsy for renal masses is a safe and effective procedure. Its ability to identify malignancy is evident, especially for primary renal tumors. However, low concordance between biopsy and definitive pathology in cases with negative biopsies, especially for tumors < 4 cm, does not reliably guarantee the absence of tumor and, therefore, strict follow-up or repeat biopsy may be indicated
The scaffold protein p140Cap limits ERBB2-mediated breast cancer progression interfering with Rac GTPase-controlled circuitries.
The docking protein p140Cap negatively regulates tumour cell features. Its relevance on
breast cancer patient survival, as well as its ability to counteract relevant cancer signalling
pathways, are not fully understood. Here we report that in patients with ERBB2-amplified
breast cancer, a p140Cap-positive status associates with a significantly lower probability of
developing a distant event, and a clear difference in survival. p140Cap dampens ERBB2-
positive tumour cell progression, impairing tumour onset and growth in the NeuT mouse
model, and counteracting epithelial mesenchymal transition, resulting in decreased metastasis
formation. One major mechanism is the ability of p140Cap to interfere with ERBB2-
dependent activation of Rac GTPase-controlled circuitries. Our findings point to a specific role
of p140Cap in curbing the aggressiveness of ERBB2-amplified breast cancers and suggest
that, due to its ability to impinge on specific molecular pathways, p140Cap may represent a
predictive biomarker of response to targeted anti-ERBB2 therapies
La revuelta de las mujeres. Un análisis de "Mooladè" de Ousmane Sembene
Fil: Stramucci, Luis Emilio. Universidad de Buenos Aires
Individual differences in Time-based Prospective Memory
Behavioral data for the research paper by Stramucci, Bisiacchi and Cantarella (under review)