Biology of a new virus isolated from Lupinus nootkatensis plants in Alaska

A new virus named Nootka lupine vein-clearing virus (NLVCV) was isolated from Lupinus nootkatensis plants that were confined to a relatively small area in the Talkeetna mountains of south-central Alaska. Annual surveys (2000–03) consistently found leaf symptoms of pronounced vein clearing and mosaic on 3- to 4-week-old plants in late June. Spherical particles ≈ 30 nm in diameter were isolated from these leaves. Virions contained a single-stranded RNA of ≈ 4·0–4·2 kb and one species of capsid protein estimated to be ≈ 40 kDa. The double-stranded RNA profile from naturally infected leaves consisted of three major bands ≈ 4·2, 1·9 and 1·5 kbp. Protein extractions from either sap or virions of diseased plants reacted to polyclonal antiserum made against the virions in Western blot assays. A predicted PCR product ≈ 500 bp was synthesized from virion RNA using primers specific to the carmovirus RNA-dependent RNA polymerase (RDRP) gene. The nucleotide sequence of the amplified DNA did not match any known virus, but contained short regions of identity to several carmoviruses. Only species belonging to the Fabaceae were susceptible to NLVCV by mechanical inoculation. Based on dsRNA profile, size of virion RNA genome and capsid protein, and similarity of the RDRP gene to that of other carmoviruses, it is suggested that NLVCV is a member of the family Tombusviridae , and tentatively of the genus Carmovirus . As the host range, RDRP gene and dsRNA profile of NLVCV are different from those of known viruses, this is a newly described plant virus

Herbage Accumulation and Nutritive Value of Limpograss Breeding Lines Under Stockpiling Management.

Supplements or conserved forage are often used to overcome forage quantity deficits for beef cattle, but stockpiled forage can be more economical. Limpograss [Hemarthria altissima (Poir.) Stapf & C.E. Hubb.] is the best available species for stockpiling in Florida because it is productive in autumn and maintains greater digestibility than other grasses at advanced stages of maturity. New limpograss hybrid breeding lines have been developed, but they have not been tested under stockpiling. Three limpograss breeding lines (1, 4F, and 10) and the most-used cultivar, Floralta, received 50 or 100 kg N ha?1 at initiation of stockpiling and herbage accumulated for 8, 12, or 16 wk. Entry 4F had greater herbage accumulation (7.3 Mg ha?1) than Entries 10, 1, and Floralta (6.1, 6.0, and 5.4 Mg ha?1, respectively). Entry 4F also had greater in vitro digestible organic matter (IVDOM) concentration (530?594 g kg?1) than Entries 1 and Floralta, but 4F was not different from Entry 10 (519?531 g kg?1) after 12 and 16 wk of accumulation. As stockpiling period increased from 8 to 16 wk, herbage accumulation increased from 5.3 to 7.4 Mg ha?1, dead material proportion increased from 1 to 10%, and herbage crude protein (CP) decreased from 44 to 32 g kg?1. Limpograss hybrids 4F and 10 are superior to Floralta for stockpiling, stockpiling period should not be longer than 12 wk, and protein supplement will be required to achieve satisfactory animal performance on stockpiled limpograss

Capim-elefante (Pennisetum purpureum Schum.), sob duas doses de nitrogênio. Consumo e produção de leite

A produção de leite, o consumo voluntário de matéria seca e a taxa de passagem da FDN no trato gastrintestinal em quatro épocas do ano (julho, outubro, janeiro e março), de vacas mestiças, sob pastejo de capim-elefante, submetido a dois níveis de nitrogênio (300 e 700 kg de N/ha•ano), foram estimados. Foi usado pastejo rotativo com três dias de ocupação e 30 de descanso, empregando-se 36 vacas lactantes mestiças Holandês x Zebu, em uma lotação de 6 vacas/ha. A estimativa de consumo e a taxa de passagem foram determinadas em apenas 24 animais. Para a coleta de extrusas, foram usadas duas vacas esôfago-fistuladas. O consumo foi estimado usando-se a relação produção fecal:indigestibilidade dos alimentos. A produção fecal foi estimada usando-se a FDN da extrusa marcada com dicromato de sódio fornecida em dose única. O consumo de MS total não foi influenciado pelas doses de N e por épocas do ano, com valores médios diários de 10,9 e 10,5 kg /MS•vaca para as doses de 300 e 700 kg N/ha•ano, respectivamente. Entretanto, o consumo de MS do capim-elefante foi influenciado por doses de N e épocas, com valores de 6,5 e 5,6 kg/vaca•dia para as doses de N supracitadas, respectivamente. O capim-elefante contribuiu com 26,0% da MS total ingerida, durante a época seca (julho/outubro) e sua contribuição aumentou para 84,0% na época das águas (janeiro/março). A produção diária de leite não foi influenciada por doses de N, com produções médias de 11,6 e 12,3 kg de leite/vaca•dia, para as doses de 300 e 700 kg N/ha•ano, respectivamente.The milk production, the dry matter intake (DMI) and the NDF passage rate in the gastrointestinal tract in four seasons (July, October, January and March), of crossbred cows, under grazing of elephant grass, submitted to two levels of nitrogen (300 and 700 kg N/ha•year) were estimated. The rotation grazing with three days of occupation and 30 days of resting, using 36 crossbred Holstein Zebu milking cows, in a stocking rate of 6 cows/ha, was used. The estimates of the intake and passage rate were determined only in 24 animals. For the extrusa collection, two esophageal fistulated cows were used. The intake was estimated using the fecal output: feed indigestibility ratio. The fecal output was estimated using the NDF of the extrusa marked with sodium dichromium fed in an unique dose. The total dry matter intake was not affected by the N levels and the year seasons, with average values of 10.9 and 10.5 kg DM/cow, for the levels of 300 and 700kg N/ha•year, respectively. However, the DM intake of the elephant grass was affected by N levels and year seasons, with reported values of 6.55 e 5.63 kg/cow•day, for the above described N levels, respectively. The elephant grass contributed with 26% of the total DM intake during the dry season (July/October) and its contribution increased up to 84% in the rainy season (January/March). The milk production was not affected by N levels, with reported average production of 11.6 and 12.3 kg/cow•day, for the levels of 300 and 700 kg N/ha•year, respectively

Conjunctive Processing of Locomotor Signals by the Ventral Tegmental Area Neuronal Population

The ventral tegmental area (VTA) plays an essential role in reward and motivation. How the dopamine (DA) and non-DA neurons in the VTA engage in motivation-based locomotor behaviors is not well understood. We recorded activity of putative DA and non-DA neurons simultaneously in the VTA of awake mice engaged in motivated voluntary movements such as wheel running. Our results revealed that VTA non-DA neurons exhibited significant rhythmic activity that was correlated with the animal's running rhythms. Activity of putative DA neurons also correlated with the movement behavior, but to a lesser degree. More importantly, putative DA neurons exhibited significant burst activation at both onset and offset of voluntary movements. These findings suggest that VTA DA and non-DA neurons conjunctively process locomotor-related motivational signals that are associated with movement initiation, maintenance and termination

The population biology of the living coelacanth studied over 21 years

Between 1986 and 2009 nine submersible and remote-operated vehicle expeditions were carried out to study the population biology of the coelacanth Latimeria chalumnae in the Comoro Islands, located in the western Indian Ocean. Latimeria live in large overlapping home ranges that can be occupied for as long as 21 years. Most individuals are confined to relatively small home ranges, resting in the same caves during the day. One hundred and forty five coelacanths are individually known, and we estimate the total population size of Grande Comore as approximately 300–400 adult individuals. The local population inhabiting a census area along an 8-km section of coastline remained stable for at least 18 years. Using LASER-assisted observations, we recorded length frequencies between 100 and 200 cm total length and did not encounter smaller-bodied individuals (\100 cm total length). It appears that coelacanth recruitment in the observation areas occur mainly by immigrating adults. We estimate that the mean numbers of deaths and newcomers are 3–4 individuals per year, suggesting that longevity may exceed 100 years. The domestic fishery represents a threat to the long-term survival of coelacanths in the study area. Recent changes in the local fishery include a decrease in the abundance of the un-motorized canoes associated with exploitation of coelacanths and an increase in motorized canoes. Exploitation rates have fallen in recent years, and by 2000, had fallen to lowest ever reported. Finally, future fishery developments are discussed