Interdisciplinary assessment of the potential for improving Integrated Pest Management practice in Scottish spring barley

Integrated Pest Management (IPM) has long been promoted as a means of reducing reliance on pesticide inputs as compared to conventional farming systems. Reduced pesticide application could be beneficial due to the links between intensive pesticide use and negative impacts upon biodiversity and human health as well as the development of pesticide resistance. Work assessing the potential of IPM in cereal production is currently limited, however, and previous findings have generally covered the subject from the perspective of either field trial data or social science studies of farmer behaviour. This thesis attempts to help to address this knowledge gap by providing a more holistic assessment of IPM in Scottish spring barley production (selected because of its dominance in Scotland’s arable production systems), in relation to three of its most damaging fungal pathogens: Rhynchosporium commune, Blumeria graminis f.sp. hordei, and Ramularia collo-cygni. Several IPM techniques of potential relevance to the sector were identified, and the prospects of three in particular – crop rotation, varietal disease resistance, and forecasting disease pressure – were assessed in several ways. Preliminary analysis of experimental field trial data collected from 2011 – 2014 across Scotland found that the majority of spring barley trials in this period (65%) did not show a statistically significant impact of fungicide treatment on yield, with the average yield increase due to fungicide application being 0.62 t/ha. This initial analysis was expanded upon using stepwise regressions of long-term (1996 – 2014) field trial data from the same dataset. Here, the difference between treated and untreated yields could be explained by disease resistance, average seasonal rainfall (whereby wetter seasons saw an increased impact of fungicide use on yield), and high combined disease severity. Stakeholder surveying provided information about current practice and attitudes towards the selected IPM techniques amongst a group of 43 Scottish spring barley farmers and 36 agronomists. Stakeholders were broadly open to taking up IPM measures on farm; sowing of disease resistant varieties was most frequently selected as the best technique in terms of both practicality and cost, though individual preference varied. However, a disparity was seen between farmer perception of their uptake of IPM and actual, self-reported uptake for both varietal disease resistance and rotation. Farmers and agronomists also overestimated the impact of fungicide use as compared with the field trials results – the majority of stakeholders believed fungicide treatment to increase yields by 1 - 2 t/ha, while the majority of 2011 – 2014 field trials had a yield difference of under 1 t/ha. The reasons behind these differences between perception and practice are not currently known. Finally, an annual survey of commercial crops, gathered from 552 farms across Scotland (from 2009 – 2015), highlighted two gaps where IPM practice could be improved upon. Firstly, relatively few of the varieties listed in the commercial crops database were highly resistant to the three diseases – 26.1% were highly resistant to Ramularia, 14.2% to Rhynchosporium, and 58.1% to mildew. Secondly, 71% of the farms included in the database had planted barley in at least two consecutive seasons, indicating that crop rotation practices could be improved. The overarching finding of this project is that there is scope for IPM uptake to be improved upon and fungicide use to be reduced while maintaining high levels of yield in Scottish spring barley production. Incorporating experimental field data, stakeholder surveying, and commercial practice data offered a unique view into the potential for IPM in this sector, and provided insights which could not have been gained through the lens of a single discipline

2D/3D ultrasonography for endometrial evaluation in a cohort of 118 postmenopausal women with abnormal uterine bleedings

Objectives: 2D/3D transvaginal ultrasonography in evaluation of endometrium in postmenopausal women with abnormal uterine bleedings (AUB). Material and methods: 2D/3D transvaginal ultrasonography (TVU) was performed in 118 menopausal women with AUB. Endometrial volume and thickness, uterine volume and endometrial vascularity were evaluated. Complete histologic evaluation of the endometrium was obtained through dilatation & curettage (D&C) and/or hysteroscopy. Accordingly, patients were divided into 3 groups: controls (no endometrial pathology, n = 49), GI (benign endometrial pathology, n = 37), GII (endometrial carcinoma, n = 32). Results: GII had greater thickness and volume of the endometrium, compared to GI and controls. The presence of arterial vascular flow was identified only in GI and GII (51.35% and 93.75%, respectively). Endometrial volume merged together with uterine volume measurements (TVU-3D) showed a strong, statistical significance between GI and GII, allowing differentiation of begin and malignant endometrial pathologies in postmenopausal women. Conclusions: In TVU diagnostics of postmenopausal women with AUB the following play the most significant role: 1) endometrial thickness (TVU-2D); 2) endometrial volume (TVU-3D); 3) uterine plus endometrial volume (TVU-3D); 4) vascularization within the endometrium, allowing to differentiate between pathological and normal endometrium (TVU-2/3D). Evaluation of the endometrial vascularity, both in TVU-2D and TVU-3D technique, does not allow for reliable differentiation between benign lesions and endometrial cancer

The impact of fungicide treatment and Integrated Pest Management on barley yields:Analysis of a long term field trials database

This paper assesses potential for Integrated Pest Management (IPM) techniques to reduce the need for fungicide use without negatively impacting yields. The impacts of three disease management practices of relevance to broad acre crops –disease resistance, forecasting disease pressure, and fungicide use – were analysed to determine impact on yield using a long-term field trials database of Scottish spring barley, with information from experiments across the country regarding yield, disease levels, and fungicide treatment. Due to changes in data collection practices, data from 1996 to 2010 were only available at trial level, while data from 2011 to 2014 were available at plot level. For this reason, data from 1996 to 2014 were analysed using regression models, while a subset of farmer relevant varieties was taken from the 2011–2014 data, and analysed using ANOVA, to provide additional information of particular relevance to current farm practice. While fungicide use reduced disease severity in 51.4%of a farmer-relevant subset of trials run 2011–2014, and yields were decreased by 0.62 t/ha on average, this was not statistically significant in 65% of trials. Fungicide use had only a minor impact on profit in these trials, with an average increase of 4.4% for malting and 4.7% for feed varieties, based on fungicide cost and yield difference; potential savings such as reduced machinery costs were not considered, as these may vary widely. Likewise, the1996–2014 database showed an average yield increase of 0.74 t/ha due to fungicide use, across a wide range of years, sites, varieties, and climatic conditions. A regression model was developed to assess key IPM and site factors which influenced the difference between treated and untreated yields across this 18-year period. Disease resistance, season rainfall, and combined disease severity of the three fungal diseases were found to be significant factors in the model. Sowing only highly resistant varieties and, as technology improves, forecasting disease pressure based on anticipated weather would help to reduce and optimise fungicide use

Perception vs practice:Farmer attitudes towards and uptake of IPM in Scottish spring barley

Integrated Pest Management (IPM) offers a suite of ways by which to reduce the need for pesticide use, thus minimising environmental damage and pathogen resistance build-up in crop production. Farmers and agronomists active in the Scottish spring barley sector were surveyed to determine the extent to which they currently use or are open to implementing three IPM measures – varietal disease resistance, crop rotation, and forecasting disease pressure – in order to control three important fungal diseases. Overall, the survey results demonstrate that farmers and agronomists are open to using the three IPM techniques. However, gaps between actual and perceived recent practice were large: despite over 60% of farmers stating that they sowed varieties highly resistant to Rhynchosporium or Ramularia, less than one third of reportedly sown varieties were highly resistant to these diseases. Similarly, over 80% of farmers indicated that they used crop rotations, yet 66% of farmers also reported sowing consecutive barley often/always. Further research is needed in order to understand why these gaps exist, and how they can be reduced in future in order to increase IPM uptake and optimise pesticide use

Impacts of Reducing UK Beef Consumption Using a Revised Sustainable Diets Framework

The impact of beef consumption on sustainability is a complex and evolving area, as sustainability covers many areas from human nutrient adequacy to ecosystem stability. Three sustainability assessment frameworks have been created to help policy makers unpack the complexities of sustainable food systems and healthy sustainable dietary change. However, none of these frameworks have yet to be applied to a case study or individual policy issue. This paper uses a hybrid version of the sustainability assessment frameworks to investigate the impact of reducing beef consumption (with a concurrent increase in consumption of plant-based foods, with a focus on legumes) on sustainability at a UK level. The aim of this paper is to understand the applicability of these overarching frameworks at the scale of an individual policy. Such an assessment is important, as this application of previously high-level frameworks to individual policies makes it possible to summarise, at a glance, the various co-benefits and trade-offs associated with a given policy, which may be of particular value in terms of stakeholder decision-making. We find that many of the proposed metrics found within the sustainability assessment frameworks are difficult to implement at an individual issue level; however, overall they show that a reduction in beef consumption and an increase in consumption of general plant-based foods, with a focus around legumes production, would be expected to be strongly beneficial in five of the eight overarching measures which were assessed

The importance of fisheries and aquaculture production for nutrition and food security

Aquatic food has a significant role to play in global nutrition and food security but is often ignored in that debate. Understanding its potential role is made difficult by the fact that aquatic food covers a large number of species which come from both capture fisheries and aquaculture and the marine and freshwater environments, including finfish, crustacea, molluscs, echinoderms, aquatic plants and other aquatic animals. Further complications arise from the fact that both supply and consumption vary significantly between countries. There are several criteria which need to be considered when discussing nutrition and food security. These include: how much food is produced, whether that production is sustainable, whether the production supports livelihoods, what the nutritional content of the food is and whether that food is safe. The authors conclude that there are many benefits to aquatic food under each of these criteria but there are also some hurdles which need to be overcome. Increased production to feed a growing global population relies on the growth of aquaculture. Limitations to such growth include the supply of raw ingredients for aquafeeds, losses due to disease outbreaks, being able to ensure high standards of food safety and overcoming environmental limitations to expansion. There are also problems with welfare conditions for people working in the supply chain which need to be addressed. Given the challenges to nutrition and food security which the world is currently facing, it is essential that aquatic food is brought into the debate and the significant benefits that aquatic foods provide are acknowledged and exploited

An interdisciplinary method for assessing IPM potential:Case study in Scottish spring barley

A method is proposed which considers Integrated Pest Management (IPM) through several lenses, in order to obtain a more holistic view of the potential for IPM, and is described using a case study of Scottish spring barley. Long-term experimental field trial databases are used to determine which management methods are best suited to the system at hand. Stakeholder engagement provides insight into which of these methods are most likely to be taken up by farmers. Finally, a database of commercial practice allows an estimate of the potential for improving management patterns, based on current levels of IPM uptake across a wider sample of Scottish farmers. Together, these diverse sources of information give a more complete view of a complex system than any individual source could and allow the identification of IPM methods which are robust, practical, and not already in widespread use in this system. Bringing together these sources of information may be of particular value for policy and other decision makers, who need information about strategies which are both practical and likely to have a large positive impact. In the case of Scottish spring barley, there is good potential to reduce the need for fungicide use through the increased use of highly resistant barley varieties

Developmental toxicity of N-methylaniline following prenatal oral administration in rats

Objectives: The objective of the study was to assess prenatal toxicity of N-methylaniline (NMA) administered by gavage to pregnant female rats. Material and Methods: Pregnant female rats were administered N-methylaniline in corn oil by gavage at daily doses of 0.8 mg/kg of body weight (b.w.), 4 mg/kg b.w., 20 mg/kg b.w. and 100 mg/kg b.w. from implantation (the 5th day post mating) to the day prior to the scheduled caesarean section (the 20th day of pregnancy). General behavior, body weight, food and water consumption, hematological, biochemical analyses and pathomorphological changes of the dams were recorded. Results: All the females survived until the end of the study. The test substance was toxic to pregnant females, even at the lowest of the used doses, i.e., 0.8 mg/kg b.w./day. Lower weight gain during pregnancy and significantly higher NMA-dose-dependent absolute weight of the organs were noted in the exposed females. The females from the groups exposed at doses of 20 mg/kg b.w./day and 100 mg/kg b.w./day developed anemia and showed higher concentrations of free thyroxine (FT3) and free triiodothyronine (FT4) thyroid hormones. Total protein concentration exhibited an increase in all the exposed groups of females. In the prenatal toxicity study, administration of N-methylaniline throughout the embryonic and fetal periods produced embryotoxic effects at doses ranging 4–100 mg/kg b.w./day. Conclusions: Considering the data obtained in this study, it is reasonable to assume that N-methylaniline administered orally to pregnant rats is toxic for mothers even at a low dose of 0.8 mg/kg b.w./day. However, this dose was not associated with any significant effects to their offspring. This prenatal exposure level may be considered as no-observed-adverse-effect level (NOAEL) for the progeny and a dose of 4 mg/kg b.w./day as the lowest-observed-adverse-effect level (LOAEL) for the progeny

Microvesicles released from ectopic endometrial foci as a potential biomarker of endometriosis

Objectives: Angiogenesis is engaged in endometriosis. It is regulated by regulatory factors and cytokines, transported in microvesicles. The purpose was to investigate the presence of MVs with vascular endothelial growth factor (VEGF) and metalloproteinase-9 (MMP-9) in peripheral blood and peritoneal fluid of women operated on for endometrioma or teratoma Material and methods: Microvesicles (MVs) were determined in blood samples and peritoneal fluid samples collected from women aged 20–60 years operated on for endometriosis (test group) and teratoma (control group). The final investigations were performed on 47 patients, who qualified for the study based on the meticulous inclusion criteria. MVs were analyzed by flow cytometry (FACS) using annexin V, antibodies for molecules characteristic of cells from endometriosis foci (keratin 18 (K18), CD105, CD146), and antibodies for intraepithelial vascular growth factor VEGF and metalloproteinase-9 (MMP-9). The sample was double “reading” using flow cytometry (FACSCantoII).  Results: Cytometry analysis confirmed MVs’ presence in plasma and peritoneal fluid collected from patients with both endometriosis and teratomas. A statistically significant higher level of AnnexinV (+) MVs were observed in plasma samples of endometriosis patients. In the control group, there was a higher percentage of double-positive VEGF (+)/MMP-9 (+) and single MMP-9 (+) positive MVs in the serum. In the peritoneal fluid higher frequency of double-positive VEGF (+)/MMP-9 (+) MVs were found in the control group. However, the amount of VEGF (+) / MMP-9 (+) MVs object did not enable to differentiate between the test and control groups. The study was the first, in which MVs were confirmed in plasma and peritoneal fluid in benign adnexa tumors.  Conclusions: Microvesicles are present in peripheral blood and peritoneal fluid samples collected from patients with endometriosis and teratomas. Microvesicles with proangiogenic factors (VEGF and MMP-9) are more abundant in blood and peritoneal fluid samples from patients with teratomas

(Nie) Zapomniana operacja Neugebauera-Le Forta. Pośrodkowe zamknięcie pochwy – bezpieczna i skuteczna procedura zabiegowego leczenia wypadania narządu płciowego u starszych kobiet

Objectives: The aim of the study is to present data concerning Neugebauer-Le Fort operations performed at the Gynecologic Oncology Clinic, Polish Mother’s Health Center Research Institute in Lodz between 2000-2009, including the effects of the surgeries on improving quality of life. Material and methods: Our research included all patients (90 women) operated due to total pelvic organ prolapse (stage IV of POP-Q) in Gynecologic Oncology Clinic. The data was collected retrospectively from medical records. Results of the treatment were evaluated based upon surveys and control checkups. The p-value of 0.05 was considered statistically significant. Results: Mean patient age was 76.5 years. The analysis of medical records showed that 35% of the subjects had a BMI index >30. Out of 90 patients, 53%, 30% and 4% of the women had 2, 3, and 4 natural deliveries, respectively, whereas 16% had forceps delivery. Of the 65 operated patients, in more than 92% all the symptoms connected with pelvic organ prolapse disappeared. Problems with the urinary tract (urinary incontinence ‘de novo’, urinary tract infections) emerged in 13% and constipation in 5% of the women. As far as improved quality of life after the surgery is concerned, 93% of the subjects answered 4 and 5 (in a scale from 1 to 5). Conclusions: Neugebauer-Le Fort surgery is characterized by high effectiveness both, in objective research and subjective ratings of the operated patients. A high safety profile constitutes a great advantage of the surgery, as was confirmed in our study. In carefully selected group of patients with stage IV of POP-Q, Neugebauer-Le Fort surgery is a safe and effective procedure.Neugebauera-Le Forta w Klinice Chirurgii Ginekologicznej ICZMP w Łodzi w latach 2000-2009, z uwzględnieniem efektów zabiegów w kontekście poprawy jakości życia. Materiał i metody: Grupę badaną stanowiły pacjentki (90 kobiet) leczone operacyjnie z powodu całkowitego wypadania narządów płciowych (IV stopień POP-Q) w Klinice Chirurgii Ginekologicznej. Dane dotyczące grupy badanej zostały uzyskane retrospektywnie z historii chorób. Wyniki leczenia oceniono na podstawie ankiet i badań kontrolnych. Dla wnioskowania statystycznego przyjęto poziom istotności p ≤ 0,05. Wyniki: Średni wiek pacjentek operowanych wyniósł 76,5 lat. Analiza dokumentacji medycznej wykazała, że u 35% kobiet wskaźnik BMI wynosił powyżej 30. Spośród 90 pacjentek: 53% miało 2 porody fizjologiczne, 30% 3 porody fizjologiczne, a 4% 4 porody fizjologiczne. Poród kleszczowy odbyło 16% kobiet. Spośród 65 pacjentek, u ponad 92%, dolegliwości związane w wypadaniem narządów płciowych ustąpiły. Nietrzymanie moczu i zaparcia nie ustąpiły po operacji u 3% kobiet. Pojawienie się problemów z układem moczowym po operacji (nietrzymanie moczu de novo, infekcja dróg moczowych) zgłosiło 13% kobiet, zaś u 5% pojawiły się zaparcia. Na pytanie o poprawę komfortu życia po operacji w skali od 1 do 5, 93% kobiet oceniło komfort na 4-5 punktów. Wnioski: Operacja Neugebauera-Le Forta charakteryzuje się wysoką skutecznością, zarówno w badaniach obiektywnych, jak i w subiektywnej ocenie pacjentek. Zaletą operacji jest wysoki profil bezpieczeństwa, potwierdzony wynikami badania. W odpowiednio dobranej grupie chorych z IV stopniem wypadania narządów płciowych operacja Neugebauera-Le Forta stanowi bezpieczną i skuteczną procedurę