Adult Emergence in Two Univoltine \u3ci\u3eCallosamia Promethea\u3c/i\u3e Populations: Preponderance of the Early Emerging Morph in the North and of the Late Emerging Morph in the South (Lepidoptera: Saturniidae)

Callosamia promethea is common on wild black cherry, Prunus serafina, at the University of Michigan Biological Station in northern lower Michigan. In this area the early emerging morph is preponderant, while to the south in northern Indiana, the late emerging morph is preponderant

Diapause and Emergence Patterns in Univoltine and Bivol Tine Populations of Promethea (Lepidoptera: Saturniidae)

Data are presented on the diapause and the seasonal emergence patterns of the adults of a univoltine Callosamia promethea population from northern Indiana and a partially bivoltine population from central Illinois. At Urbana, Illinois, the median emergence date of adults from overwintering Illinois pupae was about a month earlier than that of adults from overwintering Indiana pupae. Illinois samples had a much longer emergence period than Indiana samples. Indiana samples showed a slight tendency toward a bimodal emergence pattern, a few individuals emerging in late May and the rest emerging as a tightly synchronized group from late June to mid-July. Early emerging lIIinois moths produced mostly non.diapausing progeny, but the proportion of diapausing progeny increased as the season progressed. Some females produced both diapausing and non· diapausing progeny. Adults from non-diapausing pupae from early August to early September

Longevity and Weight Loss of Free-flying Male Cecropia Moths, \u3ci\u3eHyalophora Cecropia\u3c/i\u3e (Lepidoptera: Saturniidae)

During their spring flight season, free-ranging male cecropia moths lived a maximum of 12 days (one of 124 recaptured moths of 387 released moths). The number of survivors declined precipitiously after day five; five to seven days is probably the usual life span. The recaptured moths did not have different initial weights than those that were not recaptured. The larger the moth the more absolute weight it lost and the faster it lost weight during the first few days. A moth lost about 20% of its weight during the first night of flight and accumulated about a 40% weight loss during the remainder of its life

Antagonistic and cooperative AGO2-PUM interactions in regulating mRNAs.

Approximately 1500 RNA-binding proteins (RBPs) profoundly impact mammalian cellular function by controlling distinct sets of transcripts, often using sequence-specific binding to 3' untranslated regions (UTRs) to regulate mRNA stability and translation. Aside from their individual effects, higher-order combinatorial interactions between RBPs on specific mRNAs have been proposed to underpin the regulatory network. To assess the extent of such co-regulatory control, we took a global experimental approach followed by targeted validation to examine interactions between two well-characterized and highly conserved RBPs, Argonaute2 (AGO2) and Pumilio (PUM1 and PUM2). Transcriptome-wide changes in AGO2-mRNA binding upon PUM knockdown were quantified by CLIP-seq, and the presence of PUM binding on the same 3'UTR corresponded with cooperative and antagonistic effects on AGO2 occupancy. In addition, PUM binding sites that overlap with AGO2 showed differential, weakened binding profiles upon abrogation of AGO2 association, indicative of cooperative interactions. In luciferase reporter validation of candidate 3'UTR sites where AGO2 and PUM colocalized, three sites were identified to host antagonistic interactions, where PUM counteracts miRNA-guided repression. Interestingly, the binding sites for the two proteins are too far for potential antagonism due to steric hindrance, suggesting an alternate mechanism. Our data experimentally confirms the combinatorial regulatory model and indicates that the mostly repressive PUM proteins can change their behavior in a context-dependent manner. Overall, the approach underscores the importance of further elucidation of complex interactions between RBPs and their transcriptome-wide extent

Melanistic Males of \u3ci\u3eCallosamia Promethea\u3c/i\u3e (Lepidoptera: Saturniidae)

(excerpt) During the summer of 1983 seven melanistic male promethea moths appeared among several hundred males that emerged from cocoons we were holding in the laboratory

2 kirja Karl Morgensternile, Leipzig

http://tartu.ester.ee/record=b1840480~S1*es

The double-hit protocol induces HFpEF and impairs myocardial ubiquitin-proteasome system performance in FVB/N mice

Heart failure with preserved ejection fraction (HFpEF) is a leading cause of death and disability, with its prevalence surpassing that of heart failure with reduced ejection fraction. Obesity and hypertension are often associated with HFpEF. HFpEF can be modeled through simultaneous metabolic and hypertensive stresses in male C57BL/6N mice provoked by a combination treatment of a high-fat diet (HFD) and constitutive nitric oxide synthase inhibition by Nω-nitro-L-arginine methyl-ester (L-NAME). Ubiquitin-proteasome system (UPS) dysfunction was detected in many forms of cardiomyopathy, but whether it occurs in HFpEF remains unknown. We report successful modeling of HFpEF in male FVB/N mice and, by taking advantage of a transgenic UPS reporter mouse, we have detected myocardial UPS functioning impairment during HFpEF, suggesting a pathogenic role for impaired protein degradation in the development and progression of HFpEF

Disease-linked TDP-43 hyperphosphorylation suppresses TDP-43 condensation and aggregation

Post-translational modifications (PTMs) have emerged as key modulators of protein phase separation and have been linked to protein aggregation in neurodegenerative disorders. The major aggregating protein in amyotrophic lateral sclerosis and frontotemporal dementia, the RNA-binding protein TAR DNA-binding protein (TDP-43), is hyperphosphorylated in disease on several C-terminal serine residues, a process generally believed to promote TDP-43 aggregation. Here, we however find that Casein kinase 1 delta-mediated TDP-43 hyperphosphorylation or C-terminal phosphomimetic mutations reduce TDP-43 phase separation and aggregation, and instead render TDP-43 condensates more liquid-like and dynamic. Multi-scale molecular dynamics simulations reveal reduced homotypic interactions of TDP-43 low-complexity domains through enhanced solvation of phosphomimetic residues. Cellular experiments show that phosphomimetic substitutions do not affect nuclear import or RNA regulatory functions of TDP-43, but suppress accumulation of TDP-43 in membrane-less organelles and promote its solubility in neurons. We speculate that TDP-43 hyperphosphorylation may be a protective cellular response to counteract TDP-43 aggregation

Joseph Roth's Feuilleton Journalism as Social History in Vienna, 1919-20

From April 1919 to April 1920, twenty-four-year-old Joseph Roth worked full time as a reporter on the newly founded Viennese daily Der Neue Tag [The new day]. It was his first regular job and, although he was later to become one of the best-paid journalists of the Weimar Republic, it was also the only one he would ever hold on a fixed contract. In spring 1919, Roth had recently returned from eastern Galicia, the place of his origins, where he had also been stationed during the war, first in the infantry, then as an army press officer. His position as a Heimkehrer in Vienna after the war was precarious for a number of reasons. Paid work was scarce in the impoverished city, and Roth had not finished—indeed, would never finish—his degree. More important, although he had already been resident in Vienna as a student before the outbreak of hostilities, the “Ostjude” Roth, like so many others, had no valid papers and no right to remain in the former imperial capital. Political parties across the spectrum were agitating for the largely Jewish refugees from the former eastern provinces of the fallen Habsburg empire to be sent “home”—even by force, if necessary. Roth's position at Der Neue Tag was therefore not only an important apprenticeship for his high-profile career in journalism—which in turn laid the foundations for his oeuvre as a novelist—but also constituted a vital existential anchor. Given their historical and biographical context, it seems surprising that the texts he produced for the new newspaper—two or three a week throughout this pivotal period in Vienna's transition from self-assured imperial capital to beleaguered Social Democratic outpost—have received comparatively little attention in Roth scholarship. This is in part a result of the acknowledged bias in research on German-language culture and literature during this era toward the Weimar Republic, in particular Berlin, and away from First Republic Austria: similar texts produced slightly later by Roth in and on the German capital are often studied and seem to have eclipsed the earlier Viennese texts. This article seeks to redress the balance within Roth scholarship while also suggesting what Roth's work for Der Neue Tag can contribute to our sociohistorical understanding of the period, despite or perhaps because of the literary techniques it uses