Original Article Lack of Association of GSTT1, GSTM1, GSTO1, GSTP1 and CYP1A1 Polymorphisms for Susceptibility and Outcome in Brazilian Prostate Cancer Patients (benign prostatic hyperplasia / genetic inheritance / outcome / prostate cancer / risk factors

Abstract. The polymorphic inheritance of human drug-metabolizing enzymes, such as those encoded by the GST and CYP systems, has been implicated in both cancer risk and prognostic. In an effort to increase our understanding of the interaction between potential environmental exposure, lifestyle, and genetic factors in the predisposition and response to radiotherapy of prostate cancer patients, we examined GSTT1, GSTM1, GSTO1, GSTP1 and CYP1A1 genotypes in a Brazilian population. We studied 125 prostate cancer patients and 100 benign prostatic hyperplasia patients paired for ethnic and lifestyle characteristics. Lifetime occupational history, dietary patterns, cigarette-smoking, and other anamnestic data were obtained through interviews. Outcome was evaluated in 42 stage ≤ T2a patients presenting a Gleason score ≤ 6, PSA ≤ 10 ng/ml, treated with radiotherapy and followed up for 12 to 34 months (15 ± 8 months). None of the studied polymorphisms was found associated to prostate cancer risk either considered separately or in combination, in uni-or multivariate regression logistic analysis. Also, there was no association between genotypes and possible clinical factors of risk or any parameter of tumour aggressiveness at diagnosis or during follow-up. Patients' response to radiotherapy treatment was not associated to any genotype. In conclusion, our data suggest that GST and CYP1A1 genotypes are not associated with the susceptibility to prostate cancer or its outcome in the Brazilian population

The LBNO long-baseline oscillation sensitivities with two conventional neutrino beams at different baselines

The proposed Long Baseline Neutrino Observatory (LBNO) initially consists of $\sim 20$ kton liquid double phase TPC complemented by a magnetised iron calorimeter, to be installed at the Pyh\"asalmi mine, at a distance of 2300 km from CERN. The conventional neutrino beam is produced by 400 GeV protons accelerated at the SPS accelerator delivering 700 kW of power. The long baseline provides a unique opportunity to study neutrino flavour oscillations over their 1st and 2nd oscillation maxima exploring the $L/E$ behaviour, and distinguishing effects arising from $\delta_{CP}$ and matter. In this paper we show how this comprehensive physics case can be further enhanced and complemented if a neutrino beam produced at the Protvino IHEP accelerator complex, at a distance of 1160 km, and with modest power of 450 kW is aimed towards the same far detectors. We show that the coupling of two independent sub-MW conventional neutrino and antineutrino beams at different baselines from CERN and Protvino will allow to measure CP violation in the leptonic sector at a confidence level of at least $3\sigma$ for 50\% of the true values of $\delta_{CP}$ with a 20 kton detector. With a far detector of 70 kton, the combination allows a $3\sigma$ sensitivity for 75\% of the true values of $\delta_{CP}$ after 10 years of running. Running two independent neutrino beams, each at a power below 1 MW, is more within today's state of the art than the long-term operation of a new single high-energy multi-MW facility, which has several technical challenges and will likely require a learning curve.Comment: 21 pages, 12 figure

Comparação termohigrométrica de sub-altitude em área urbana e rural em São Carlos, Brasil, por meio de VANT/DRONE.

A Radiossondagem de sub-altitude tem como objetivo mensurar os dados climatológicos em vários níveis verticais da atmosfera por meio de um equipamento denominado radiossonda. Além do mais, é conhecido que os diferentes tipos de uso e ocupação do solo (urbano, industrial, rural, florestal) alteram o balanço de energia entre a superfície e a atmosfera. Dessa forma, o estudo proposto tem como objetivo analisar e comparar os valores de temperatura e umidade relativa do ar próximo a superfície (1,5m de altura) e em diferentes alturas (50m e 190m da superfície) em área urbana e rural no município de São Carlos, Brasil, no período noturno em episódios de inverno, por meio de termohigrômetros acoplados em um Veículo Aéreo Não-Tripulado (Vant/Drone) do tipo quadricóptero (quatro hélices). O voo na área urbana foi realizado no dia 13/07/2018 e na área rural no dia 26/07/2018 entre 19:30 e 20:30. Os resultados demonstraram que na área urbana em períodos noturnos a temperatura e umidade relativa do ar são maiores próxima a superfície em relação aos dados de sub-altitude. Já na área rural em períodos noturnos a temperatura do ar é menor e a umidade relativa do ar é maior próximo a superfície em comparação aos dados de sub-altitude

The Clinical Use Of Thyroid Function Tests [utilização Dos Testes De Função Tireoidiana Na Prática Clínica]

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Nested-PCR using MPB64 fragment improves the diagnosis of pleural and meningeal tuberculosis Nested-PCR usando o fragmento MPB64 melhora o diagnóstico da tuberculose pleural e meníngea

Fluids in which Mycobacterium tuberculosis are seldom found, such as pleural and cerebrospinal liquids, are good candidates to be studied using PCR techniques. We detail our experience with a PCR assay applied to pleural and cerebrospinal fluids using the primer MPB64. Seventy three specimens were analyzed: 30 pleural fluids (PF), 26 pleural biopsies (PB) and 17 cerebrospinal fluids (CSF). The gold standard for the diagnosis of tuberculous meningitis was the positive culture for M. tuberculosis in CSF. Tuberculous pleural effusion was diagnosed when cultures of PF and/or PB were positive for M. tuberculosis, or the PB histology showed granulomas. Our results, compared to the gold standards employed, showed a sensitivity of 70%, specificity of 88%, positive predictive value of 82% and negative predictive value of 80%. The high specificity of the MPB64 fragment while still retaining a good sensitivity makes it very well suited for pleural and cerebrospinal tuberculosis diagnosis.<br>O Mycobacterium tuberculosis é raramente encontrado em fluidos como o líquido pleural e o cérebroespinhal, tornando estas localizações de difícil diagnóstico. Apresentamos nossa experiência com uma técnica de PCR aplicada a líquido pleural e cerebroespinhal com o uso do primer MPB64. Sessenta e três espécimes foram analisados: 30 líquidos pleurais (PF), 26 biópsias pleurais (PB) e 17 líquidos cerebroespinhais (CSF). O gold standard para o diagnóstico de meningite tuberculosa foi a cultura positiva para M. tuberculosis no CSF. Tuberculose pleural era diagnosticada quando culturas do PF e/ou PB eram positivas para M. tuberculosis, ou a histologia da PB mostrava granulomas. Nossos resultados, comparados aos gold standards empregados, mostram sensitividade de 70%, especificidade de 88%, valor preditivo positivo de 82% e valor preditivo negativo de 80%. A elevada especificidade e boa sensibilidade do fragmento MPB64 o transformam em um bom parâmetro para o diagnóstico de tuberculose pleural e do líquido cérebroespinhal