Multimodal Imaging of Photoreceptor Structure in Choroideremia

Purpose Choroideremia is a progressive X-linked recessive dystrophy, characterized by degeneration of the retinal pigment epithelium (RPE), choroid, choriocapillaris, and photoreceptors. We examined photoreceptor structure in a series of subjects with choroideremia with particular attention to areas bordering atrophic lesions. Methods Twelve males with clinically-diagnosed choroideremia and confirmed hemizygous mutations in the CHM gene were examined. High-resolution images of the retina were obtained using spectral domain optical coherence tomography (SD-OCT) and both confocal and non-confocal split-detector adaptive optics scanning light ophthalmoscope (AOSLO) techniques. Results Eleven CHM gene mutations (3 novel) were identified; three subjects had the same mutation and one subject had two mutations. SD-OCT findings included interdigitation zone (IZ) attenuation or loss in 10/12 subjects, often in areas with intact ellipsoid zones; RPE thinning in all subjects; interlaminar bridges in the imaged areas of 10/12 subjects; and outer retinal tubulations (ORTs) in 10/12 subjects. Only split-detector AOSLO could reliably resolve cones near lesion borders, and such cones were abnormally heterogeneous in morphology, diameter and density. On split-detector imaging, the cone mosaic terminated sharply at lesion borders in 5/5 cases examined. Split-detector imaging detected remnant cone inner segments within ORTs, which were generally contiguous with a central patch of preserved retina. Conclusions Early IZ dropout and RPE thinning on SD-OCT are consistent with previously published results. Evidence of remnant cone inner segments within ORTs and the continuity of the ORTs with preserved retina suggests that these may represent an intermediate state of retinal degeneration prior to complete atrophy. Taken together, these results supports a model of choroideremia in which the RPE degenerates before photoreceptors

Microscopic Inner Retinal Hyper-reflective Phenotypes in Retinal and Neurologic Disease

Purpose. We surveyed inner retinal microscopic features in retinal and neurologic disease using a reflectance confocal adaptive optics scanning light ophthalmoscope (AOSLO). Methods. Inner retinal images from 101 subjects affected by one of 38 retinal or neurologic conditions and 11 subjects with no known eye disease were examined for the presence of hyper-reflective features other than vasculature, retinal nerve fiber layer, and foveal pit reflex. The hyper-reflective features in the AOSLO images were grouped based on size, location, and subjective texture. Clinical imaging, including optical coherence tomography (OCT), scanning laser ophthalmoscopy, and fundus photography was analyzed for comparison. Results. Seven categories of hyper-reflective inner retinal structures were identified, namely punctate reflectivity, nummular (disc-shaped) reflectivity, granular membrane, waxy membrane, vessel-associated membrane, microcysts, and striate reflectivity. Punctate and nummular reflectivity also was found commonly in normal volunteers, but the features in the remaining five categories were found only in subjects with retinal or neurologic disease. Some of the features were found to change substantially between follow up imaging months apart. Conclusions. Confocal reflectance AOSLO imaging revealed a diverse spectrum of normal and pathologic hyper-reflective inner and epiretinal features, some of which were previously unreported. Notably, these features were not disease-specific, suggesting that they might correspond to common mechanisms of degeneration or repair in pathologic states. Although prospective studies with larger and better characterized populations, along with imaging of more extensive retinal areas are needed, the hyper-reflective structures reported here could be used as disease biomarkers, provided their specificity is studied further

Characterization and comparison of enterococcus spp. Isolates from feces of healthy dogs and urine of dogs with utis

Enterococcus spp. are opportunistic pathogens of both humans and animals characterized by high resistance to antimicrobials. Dogs could be intestinal carriers or suffer from Enterococcus infections, mainly urinary tract infections (UTIs). This study aimed to analyze and compare En-terococcus spp. isolated from healthy dog stools and sick dog urine. Overall, 51 isolates (29 from stools and 22 from UTI) were characterized at species level and tested for antimicrobial resistance, biofilm production and presence of resistance and virulence genes. E. faecium and E. faecalis resulted as equally distributed in stools samples, while E. faecalis predominated among UTI isolates. HLAR phenotype was detected in 47.1% isolates; 64.7% isolates were resistant to ampicillin (47.1% with a MIC ≥ 64 µg/mL). High levels of resistance were recorded for fluoroquinolones (enrofloxacin 74.5%, ciprofloxacin 66.7%), clindamycin (84.3%), tetracycline (78.4%) and quinupristin–dalfopristin (78.4%). No vancomycin resistant strains were detected. All but one isolate were multidrug-resistant. Most detected resistance genes were tetM (70.5%), pbp4 (52.9%) and aph(3′ )-IIIa (39.2%). All isolates were able to produce biofilm, but isolates from UTIs and belonging to E. faecalis more frequently resulted in strong biofilm producers. Most detected virulence genes were asa1 (52.9%), gelE (41.2%), cylA (37.3%) and esp (35.3%); all of them resulted as more frequently associated to E. faecalis. No particular differences emerged between isolates from feces and UTI, considering all evaluated aspects. Our results confirm pet dogs as carriers of multidrug-resistant enterococci; stool microflora could be considered as the most probable source of enterococcal UTI and E. faecalis carried by dogs seems to be more virulent than E. faecium, justifying its more frequent involvement in urinary tract infections

Marginally low mass ratio close binary system V1191 Cyg

In this study, we present photometric and spectroscopic variations of the extremely small mass ratio ($q\simeq 0.1$) late-type contact binary system \astrobj{V1191 Cyg}. The parameters for the hot and cooler companions have been determined as $M_\textrm{h}$ = 0.13 (1) $M_{\odot}$, $M_\textrm{c}$ = 1.29 (8) $M_{\odot}$, $R_\textrm{h}$ = 0.52 (15) $R_{\odot}$, $R_\textrm{c}$ = 1.31 (18) $R_{\odot}$, $L_\textrm{h}$ = 0.46 (25) $L_{\odot}$, $L_\textrm{c}$ = 2.71 (80) $L_{\odot}$, the separation of the components is $a$= 2.20(8) $R_{\odot}$ and the distance of the system is estimated as 278(31) pc. Analyses of the times of minima indicates a period increase of $\frac{dP}{dt}=1.3(1)\times 10^{-6}$ days/yr that reveals a very high mass transfer rate of $\frac{dM}{dt}=2.0(4)\times 10^{-7}$$M_{\odot}$/yr from the less massive component to the more massive one. New observations show that the depths of the minima of the light curve have been interchanged.Comment: Accepted for publication in New Astronomy, 16 pages, 2 figures, 4 table

Four ultra-short period eclipsing M-dwarf binaries in the WFCAM Transit Survey

We report on the discovery of four ultra-short period (P<0.18 days) eclipsing M-dwarf binaries in the WFCAM Transit Survey. Their orbital periods are significantly shorter than of any other known main-sequence binary system, and are all significantly below the sharp period cut-off at P~0.22 days as seen in binaries of earlier type stars. The shortest-period binary consists of two M4 type stars in a P=0.112 day orbit. The binaries are discovered as part of an extensive search for short-period eclipsing systems in over 260,000 stellar lightcurves, including over 10,000 M-dwarfs down to J=18 mag, yielding 25 binaries with P<0.23 days. In a popular paradigm, the evolution of short period binaries of cool main-sequence stars is driven by loss of angular momentum through magnetised winds. In this scheme, the observed P~0.22 day period cut-off is explained as being due to timescales that are too long for lower-mass binaries to decay into tighter orbits. Our discovery of low-mass binaries with significantly shorter orbits implies that either these timescales have been overestimated for M-dwarfs, e.g. due to a higher effective magnetic activity, or that the mechanism for forming these tight M-dwarf binaries is different from that of earlier type main-sequence stars.Comment: 22 pages, 17 figures, 3 tables Accepted for publication in MNRA

Multifunctional Eu-doped NaGd(MoO4)(2) nanoparticles functionalized with poly(L-lysine) for optical and MRI imaging

A method for the synthesis of non-aggregated and highly uniform Eu3+ doped NaGd(MoO4)(2) nanoparticles is reported for the first time. The obtained particles present tetragonal structure, ellipsoidal shape and their size can be varied by adjusting the experimental synthesis parameters. These nanoparticles, which were coated with citrate anions and functionalised with PLL, have also been developed in order to improve their colloidal stability in physiological medium (2-(N-morpholino) ethanesulfonic acid, MES). A study of the luminescent dynamics of the samples as a function of the Eu doping level has been conducted in order to find the optimum nanophosphors, whose magnetic relaxivity and cell viability have also been evaluated for the first time for this system, in order to assess their suitability as multifunctional probes for optical (in vitro) and magnetic bioimaging applications

Reduced magnetic braking and the magnetic capture model for the formation of ultra-compact binaries

A binary in which a slightly evolved star starts mass transfer to a neutron star can evolve towards ultra-short orbital periods under the influence of magnetic braking. This is called magnetic capture. In a previous paper we showed that ultra-short periods are only reached for an extremely small range of initial binary parameters, in particular orbital period and donor mass. Our conclusion was based on one specific choice for the law of magnetic braking, and for the loss of mass and angular momentum during mass transfer. In this paper we show that for less efficient magnetic braking it is impossible to evolve to ultra-short periods, independent of the amount of mass and associated angular momentum lost from the binary.Comment: 7 pages, 7 figures, accepted for publication in Astronomy and Astrophysics. See http://www.astro.uu.nl/~sluys/PhD

The Lorentz force in atmospheres of CP stars: θ\theta Aurigae

Several dynamical processes may induce considerable electric currents in the atmospheres of magnetic chemically peculiar (CP) stars. The Lorentz force, which results from the interaction between the magnetic field and the induced currents, modifies the atmospheric structure and induces characteristic rotational variability of the hydrogen Balmer lines. To study this phenomena we have initiated a systematic spectroscopic survey of the Balmer lines variation in magnetic CP stars. In this paper we continue presentation of results of the program focusing on the high-resolution spectral observations of A0p star \aur (HD 40312). We have detected a significant variability of the H$\alpha$, H$\beta$, and H$\gamma$ spectral lines during full rotation cycle of the star. This variability is interpreted in the framework of the model atmosphere analysis, which accounts for the Lorentz force effects. Both the inward and outward directed Lorentz forces are considered under the assumption of the axisymmetric dipole or dipole+quadrupole magnetic field configurations. We demonstrate that only the model with the outward directed Lorentz force in the dipole+quadrupole configuration is able to reproduce the observed hydrogen line variation. These results present new strong evidences for the presence of non-zero global electric currents in the atmosphere of an early-type magnetic star.Comment: 10 figure

Nitrogen turnover in the leaf litter and fine roots of sugar maple

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/117199/1/ecy201091123456.pd