A fluorescent mutant of the phosphorus-solubilizing fungus Penicillium bilaiae to image rhizosphere growth

Non-Peer ReviewedNovozymes manufactures and markets a phosphate inoculant, JumpStart®, based on the phosphorus-solubilizing fungus Penicillium bilaiae. We are developing a mutant of this fungus that expresses the DsRed-Express fluorescent protein, which will be used as a research tool to allow us to see where and how P. bilaiae interacts with plant roots

Genomic sequence of a ranavirus isolated from pike-perch Sander lucioperca

The pike-perch iridovirus (PPIV) was isolated in Finland from apparently healthy pike-perch fingerlings during routine disease surveillance. Our phylogenomic analysis revealed that PPIV is the first fish member of a clade of ranaviruses previously described from European and Chinese amphibians

Efficient red electroluminescence from diketopyrrolopyrrole copolymerised with a polyfluorene

We thank the EC Seventh Framework Programme (FP7/2007-2013) under Grant Agreement No. 212311 (ONE-P), the RTN THREADMILL (EU-Contract No.: MRTN-CT-2006-036040), the ITNs SUPERIOR (PITN-CT-2009-238177), and CONTEST (PITN-CT-2012-317488) as well as the Royal Society, and EPSRC for funding

Crustal Structure in the Southern Apennines from Teleseismic Receiver Functions

While the upper structure of the Southern Apennines is known, lack of control on the deep structure allows competing thin-skin and tick-skin models of the orogen. In thin-skin models the detachment decouples a stack of rootless nappes from the basement. In the tick-skin models, besement is involved in the most recent phase of thrusting. To examine crustal structure, we use teleseismic data from the CAT/SCAN array in southern Italy. We use receiver functions (RF) processed into a Common Conversion Point (CCP) stack to generate images of the crust. Interpretation and correlation to geological structure is done using inversions of individual station RFs. We focus on a shallow discontinuity where P-to-S conversions occur. In the foreland, it corresponds to velocity jumps between carbonate and clastic strata with basement. A similar interpretation for the Apennines provides the most parsimonious explanation and supports a tick-skin interpretation. In a thick-skin reconstruction, the amount of shortening is much smaller than for a thin-skin model. This implies considerably less Plio-Pleistocene shortening across the Apennines and suggests an E-SE motion of the Calabrian Arc subparallel to the southern Apennines rather than a radial expansion of the Arc

Process evaluation results from a school- and community-linked intervention: the Trial of Activity for Adolescent Girls (TAAG)

Process evaluation is a component of intervention research that evaluates whether interventions are delivered and received as intended. Here, we describe the process evaluation results for the Trial of Activity for Adolescent Girls (TAAG) intervention. The intervention consisted of four synergistic components designed to provide supportive school- and community-linked environments to prevent the decline in physical activity in adolescent girls. Process evaluation results indicate that the intervention components were delivered from intervention staff to teachers with high fidelity (84–97%) to the protocol and with lower fidelity (range: 18–93%) from teachers to students. Physical activity programs for girls, a unique feature of the TAAG intervention, increased from a mean of 10 programs per school to a mean of 16 and 15 in years 1 and 2, respectively, in intervention schools, with no change in control schools. These findings suggest that a multicomponent school- and community-based physical activity intervention can be delivered with fidelity and result in a middle school environment that supports physical activity for girls

Illumination of the Crustal Structure in the Southern Apennines using Teleseismic Receiver Functions, CAT/SCAN Project

Field geology, well data and seismic imaging have illuminated the upper crustal structure of the Southern Apennines. However, lack of control of the deep structure allows viable competing thin-skin and thick-skin models of the orogen. In thin-skin models the detachment decouples a stack of rootless nappes from the basement. In thick-skin models, basement is involved in the most recent phase of thrusting. To examine the deep crustal structure, we use the teleseismic recordings from the CAT/SCAN array, deployed in southern Italy from Dec. 2003-Oct. 2005. We use receiver functions processed into a Common Conversion Point stack to generate images of the crust. We image three main westward-dipping seismic-velocity discontinuities where P-to-S conversions occur. They correspond to velocity jumps at the Moho, the upper-lower crust boundary and sedimentary interfaces resulting from the contrast between clastic and carbonate strata with basement. The CCP image matches features from both thin-skin and thick skin model. The lateral continuity of the converters favors thin skin, but consistent interpretation across the image favors the thick skin. Overall, the results provide a better fit to the thick-skin interpretation. This suggests a change in structural style as the collision with Apulia halted motion. This model also implies considerably less Plio-Pleistocene shortening across the Apennines and a SE motion of the Calabrian Arc subparallel to the southern Apennines rather than a radial expansion of the Arc

Steady late quaternary slip rate on the Cinarcik section of the North Anatolian fault near Istanbul, Turkey

The distribution of plate motion between multiple fault strands and how this distribution may evolve remain poorly understood, despite the key implications for seismic hazards. The North Anatolian Fault in northwest Turkey is a prime example of a multistranded continental transform. Here we present the first constraints on late Quaternary slip rates on its northern branch across the Cinarcik Basin in the eastern Marmara Sea. We use both deep penetration and high‐resolution multichannel seismic reflection data with a stratigraphic age model to show that a depocenter has persisted near the fault bend responsible for that transform basin. Successively older depocenters have been transported westward by fault motion relative to Eurasia, indicating a uniform right‐lateral slip rate of 18.5 mm/yr over the last 500,000 years, compared to overall GPS rates (23–24 mm/yr). Thus, the northern branch has slipped at a nearly constant rate and has accounted for most of the relative plate motion between Eurasia and Anatolia since ~0.5 Ma

Protocol for a systematic review of guidelines for rigour in the design, conduct and analysis of biomedical experiments involving laboratory animals

Objective: Within the last years, there has been growing awareness of the negative repercussions of unstandardized planning, conduct and reporting of preclinical and biomedical research. Several initiatives have set the aim of increasing validity and reliability in reporting of studies and publications, and publishers have formed similar groups. Additionally, several groups of experts across the biomedical spectrum have published experience and opinion-based guidelines and guidance on potential standardized reporting. While all these guidelines cover reporting of experiments, an important step prior to this should be rigours planning and conduction of studies. The aim of this systematic review is to identify and harmonize existing experimental design, conduct and analysis guidelines relating to internal validity and reproducibility of preclinical animal research. The review will also identify literature describing risks of bias pertaining to the design, conduct and analysis of preclinical biomedical research. Search strategy: PubMed, Embase and Web of Science will be searched systematically to identify guidelines published in English language in peer-reviewed journals before January 2018 (box 1). All articles or systematic reviews in English language that describe or review guidelines on the internal validity and reproducibility of animal studies will be included. Google search for guidelines published on the websites of major funders and professional organisations can be found in (Box 2). Screening and annotation: Unique references will be screened in two phases: screening for eligibility based on title and abstract, followed by screening for definitive inclusion based on full text. Screening will be performed in SyRF (http://syrf.org.uk). Each reference will be randomly presented to two independent reviewers. Disagreements between reviewers will be resolved by additional screening of the reference by a third, senior researcher. Data management and reporting: All data, including extracted text and guidelines, will be stored in the SyRF platform. Elements of the included guidelines will be identified using a standardized extraction form. Reporting will follow the PRISMA guidelines as far as applicable

Using estrus detection patches to optimally time insemination improved pregnancy risk in suckled beef cows enrolled in a fixed-time artificial insemination program

Citation: Hill, S. L., Grieger, D. M., Olson, K. C., Jaeger, J. R., Dahlen, C. R., Bridges, G. A., . . . Stevenson, J. S. (2016). Using estrus detection patches to optimally time insemination improved pregnancy risk in suckled beef cows enrolled in a fixed-time artificial insemination program. Journal of Animal Science, 94(9), 3703-3710. doi:10.2527/jas2016-0469A multilocation study examined pregnancy risk (PR) after delaying AI in suckled beef cows from 60 to 75 h when estrus had not been detected by 60 h in response to a 7-d CO-Synch + progesterone insert (CIDR) timed AI (TAI) program (d-7: CIDR insert concurrent with an injection of GnRH; d 0: PGF(2 alpha) injection and removal of CIDR insert; and GnRH injection at TAI [ 60 or 75 h after CIDR removal]). A total of 1,611 suckled beef cows at 15 locations in 9 states (CO, IL, KS, MN, MS, MT, ND, SD, and VA) were enrolled. Before applying the fixed-time AI program, BCS was assessed, and blood samples were collected. Estrus was defined to have occurred when an estrus detection patch was >50% colored (activated). Pregnancy was determined 35 d after AI via transrectal ultrasound. Cows (n = 746) detected in estrus by 60 h (46.3%) after CIDR removal were inseminated and treated with GnRH at AI (Control). Remaining nonestrous cows were allocated within location to 3 treatments on the basis of parity and days postpartum: 1) GnRH injection and AI at 60 h (early-early = EE; n = 292), 2) GnRH injection at 60 h and AI at 75 h (early-delayed = ED; n = 282), or 3) GnRH injection and AI at 75 h (delayed-delayed = DD; n = 291). Control cows had a greater (P < 0.01) PR (64.2%) than other treatments (EE = 41.7%, ED = 52.8%, DD = 50.0%). Use of estrus detection patches to delay AI in cows not in estrus by 60 h after CIDR insert removal (ED and DD treatments) increased (P < 0.05) PR to TAI when compared with cows in the EE treatment. More (P < 0.001) cows that showed estrus by 60 h conceived to AI at 60 h than those not showing estrus (64.2% vs. 48.1%). Approximately half (49.2%) of the cows not in estrus by 60 h had activated patches by 75 h, resulting in a greater (P < 0.05) PR than their nonestrous herd mates in the EE (46.1% vs. 34.5%), ED (64.2% vs. 39.2%), and DD (64.8% vs. 31.5%) treatments, respectively. Overall, cows showing estrus by 75 h (72.7%) had greater (P < 0.001) PR to AI (61.3% vs. 37.9%) than cows not showing estrus. Use of estrus detection patches to allow for a delayed AI in cows not in estrus by 60 h after removal of the CIDR insert improved PR to TAI by optimizing the timing of the AI in those cows

An exploratory randomised controlled trial of a premises-level intervention to reduce alcohol-related harm including violence in the United Kingdom

&lt;b&gt;Background&lt;/b&gt;&lt;p&gt;&lt;/p&gt; To assess the feasibility of a randomised controlled trial of a licensed premises intervention to reduce severe intoxication and disorder; to establish effect sizes and identify appropriate approaches to the development and maintenance of a rigorous research design and intervention implementation.&lt;p&gt;&lt;/p&gt; &lt;b&gt;Methods&lt;/b&gt;&lt;p&gt;&lt;/p&gt; An exploratory two-armed parallel randomised controlled trial with a nested process evaluation. An audit of risk factors and a tailored action plan for high risk premises, with three month follow up audit and feedback. Thirty-two premises that had experienced at least one assault in the year prior to the intervention were recruited, match paired and randomly allocated to control or intervention group. Police violence data and data from a street survey of study premises’ customers, including measures of breath alcohol concentration and surveyor rated customer intoxication, were used to assess effect sizes for a future definitive trial. A nested process evaluation explored implementation barriers and the fidelity of the intervention with key stakeholders and senior staff in intervention premises using semi-structured interviews.&lt;p&gt;&lt;/p&gt; &lt;b&gt;Results&lt;/b&gt;&lt;p&gt;&lt;/p&gt; The process evaluation indicated implementation barriers and low fidelity, with a reluctance to implement the intervention and to submit to a formal risk audit. Power calculations suggest the intervention effect on violence and subjective intoxication would be raised to significance with a study size of 517 premises.&lt;p&gt;&lt;/p&gt; &lt;b&gt;Conclusions&lt;/b&gt;&lt;p&gt;&lt;/p&gt; It is methodologically feasible to conduct randomised controlled trials where licensed premises are the unit of allocation. However, lack of enthusiasm in senior premises staff indicates the need for intervention enforcement, rather than voluntary agreements, and on-going strategies to promote sustainability