Orbital eccentricity of the symbiotic star MWC 560

We present projected rotational velocity measurements of the red giant in the symbiotic star MWC 560 (V694 Mon), using the high-resolution spectroscopic observations with the FEROS spectrograph. We find that the projected rotational velocity of the red giant is vsini = 8.2 +/- 1.5 km/s, and estimate its rotational period to be P_rot = 144-306 days. Using the theoretical predictions of tidal interaction and pseudosynchronization, we estimate the orbital eccentricity e=0.68-0.82. We briefly discuss the connection of our results with the photometric variability of the object.Comment: AN, accepte

Asteroseismology of the {\it Kepler} target KIC\,9204718

The high precision data obtained by the {\it Kepler} satellite allows us to detect hybrid type pulsator candidates more accurately than the data obtained by ground-based observations. In this study, we present preliminary results on the new analysis of the {\it Kepler} light curve and high resolution spectroscopic observations of pulsating Am star KIC\,9204718. Our tentative analysis therefore show that the star has hybrid pulsational characteristics.Comment: 'Proceedings of Wide Field variability surveys : a 21 st Century 22nd Los Alamos Stellar Pulsation Conference San Pedro De Atacama ,Chile Nov 28-Dec 2, 2016' to be published by the EPJ Web of Conference

Photometric and spectroscopic variability of 53 Per

A new investigation of the variability of the SPB-type star 53 Per is presented. The analysis of the BRITE photometry allowed us to determine eight independent frequencies and the combination one. Five of these frequencies and the combination one were not known before. In addition, we gathered more than 1800 new moderate and high-resolution spectra of 53 Per spread over approximately six months. Their frequency analysis revealed four independent frequencies and the combination one, all consistent with the BRITE results.Comment: 2 pages, accepted for publication in the Proceedings of the PAS (Proc. of the 2nd BRITE Science conference, Innsbruck

Analysis of predictive thermodynamic models for estimation of polycyclic aromatic solid solubility in hot pressurized water

The ability of two thermodynamic approaches to predict the solubility of solid compounds in hot pressurized water is studied and compared. The Regular Solution Theory, based on the solubility parameter concept, and UNIFACbased models were applied to calculate the solute activity coefficient and then, solubility predictions were compared with experimental data reported in the literature. The analysis was carried out considering polycyclic aromatic hydrocarbons as model substances, i.e. substances which contain only the aromatic AC and ACH groups, and for which reliable pure physical properties such as melting point, fusion enthalpy and molar volume are available in the literature. The solubility values predicted with the UNIFAC-based models were considerably better than those obtained with the solubility parameter approach. Particularly, the modified Dortmund UNIFAC model presented an appropriate functionality of solubility with temperature, and the extension of this model to other type of aromatic compounds also provided a satisfactory prediction of solubility data.This work has been financed by project S2009-AGR-1469 from the Comunidad Autónoma de Madrid (Spain) and CSD2007-00063 FUN-CFOOD (Programa CONSOLIDERINGENIO 2010) project

O-GlcNAcylation of the human epidermal growth factor receptor

The reversible O-linked attachment of single β-D-N-acetylglucosamine (GlcNAc) moieties to serine/threonine residues in target proteins is a frequently occurring post-translational modification affecting the functionality of many cellular systems. In this report we present experimental evidence suggesting that the epidermal growth factor receptor (EGFR) is subjected to O-GlcNAcylation in human carcinoma epidermoid A431 cells and human lung carcinoma A549 cells. However, no signal was detected in human cervix adenocarcinoma HeLa cells or in mouse EGFR-T17 fibroblasts ectopically expressing the human EGFR. We detected a positive O-GlcNAcylation signal in the immunoprecipitated EGFR by Western blotting using two distinct specific anti-O-GlcNAc antibodies even after N-deglycosylation of the receptor using peptide-N-glycosidase F (PNGase F). Conversely, the presence of EGFR was detected by Western blotting using an anti-EGFR antibody in the immunocomplex of O-GlcNAcylated proteins immunoprecipitated with an anti-O-GlcNAc antibody. These signals were enhanced when the O-linked β-N-acetylglucosaminidase (OGA) inhibitor Thiamet G was added to prevent the deglycosylation of the GlcNAc moiety(ies). Moreover, we also detected a positive signal in the immunoprecipitated and N-deglycosylated EGFR using PNGase F, and tunicamycin when the cells were metabolically labeled with azido-GlcNAc (GlcNAz), biotinylated and probed with a streptavidin-labeled peroxidase. Finally, EGFR and O-linked β-N-acetylglucosamine transferase (OGT) co-immunoprecipitate, and incubation of the immunoprecipitated EGFR with the immunoprecipitated OGT in the presence of uridine 5′-diphospho-N-acetylglucosamine (UDP-GlcNAc) resulted in a significant enhancement of the EGFR O-GlcNAcylation signal as detected by Western blotting using an anti-O-GlcNAc antibody. We conclude that the human EGFR is subjected to O-GlcNAcylation in the A431 and A549 tumor cell lines.This work was funded by grants to AV from the European Commission (contract no. PITN-GA-2011-289033), the Secretaría de Estado de Investigación, Desarrollo e Innovación (SAF2011-23494 and SAF2014-52048-R), and the Consejería de Educación de la Comunidad de Madrid (S2011/BMD-2349). SRS received funding from the People Program (Marie Curie Actions) of the European Union’s Seventh Framework Program FP7/2007-2013 under REA grant agreement PITN-GA-2011-289033.Peer Reviewe

Experimental Measurements and Correlation of the Solubility of Three Primary Amides in Supercritical CO2: Acetanilide, Propanamide, and Butanamide

Solubilities of three primary amides, namely, acetanilide, propanamide, and butanamide, in supercritical carbon dioxide were measured at T = (308.2, 313.2, and 323.2) K over the pressure range (9.0 to 40.0) MPa by a flow type apparatus. The solubility behavior of the three solids shows an analogous trend with a crossover region of the respective isotherms between (12 to 14) MPa. The solubility of each amide, at the same temperature and pressure, decreases from propanamide to acetanilide. Pure compound properties required for the modeling were estimated, and the solubilities of the amides were correlated by using the Soave-Redlich-Kwong cubic equation of state with an absolute average relative deviation (AARD) from (1.3 to 6.1) %

Analysis of predictive thermodynamic models for estimation of polycyclic aromatic solid solubility in hot pressurized water

This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License.The ability of two thermodynamic approaches to predict the solubility of solid compounds in hot pressurized water is studied and compared. The Regular Solution Theory, based on the solubility parameter concept, and UNIFACbased models were applied to calculate the solute activity coefficient and then, solubility predictions were compared with experimental data reported in the literature. The analysis was carried out considering polycyclic aromatic hydrocarbons as model substances, i.e. substances which contain only the aromatic AC and ACH groups, and for which reliable pure physical properties such as melting point, fusion enthalpy and molar volume are available in the literature. The solubility values predicted with the UNIFAC-based models were considerably better than those obtained with the solubility parameter approach. Particularly, the modified Dortmund UNIFAC model presented an appropriate functionality of solubility with temperature, and the extension of this model to other type of aromatic compounds also provided a satisfactory prediction of solubility data.This work has been financed by project S2009-AGR- 1469 from the Comunidad Autónoma de Madrid (Spain) and CSD2007-00063 FUN-CFOOD (Programa CONSOLIDERINGENIO 2010) project.Peer Reviewe

On the solubility of three disperse anthraquinone dyes in supercritical carbon dioxide: new experimental data and correlation

Solubility measurements of quinizarin. (1,4-dihydroxyanthraquinone), disperse red 9 (1-(methylamino) anthraquinone), and disperse blue 14 (1,4-bis(methylamino)anthraquinone) in supercritical carbon dioxide (SC CO2) were carried out in a flow type apparatus, at a temperature range from (333.2 to 393.2) K and at pressures from (12.0 to 40.0) MPa. Mole fraction solubility of the three dyes decreases in the order quinizarin (2.9 x 10(-6) to 2.9.10(-4)), red 9 (1.4 x 10(-6) to 3.2 x 10(-4)), and blue 14 (7.8 x 10(-8) to 2.2 x 10(-5)). Four semiempirical density based models were used to correlatethe solubility of the dyes in the SC CO2. From the correlation results, the total heat of reaction, heat of vaporization plus the heat of solvation of the solute, were calculated and compared with the results presented in the literature. The solubilities of the three dyes were correlated also applying the Soave-Redlich-Kwong cubic equation of state (SRK CEoS) with classical mixing rules, and the physical properties required for the modeling were estimated and reported