Intérêt diagnostique du dosage de la Copeptine chez les patients se présentant aux urgences pour une douleur thoracique suspecte de syndrome coronarien aigu, sans élévation du segment ST et avec une Troponine inférieure au 99ème percentile à l'admission

L'objectif principal de notre étude était d'évaluer la valeur diagnostic de la copeptine ultrasensible chez les patients admis aux urgences pour une douleur thoracique avec un ECG non ST+ et un dosage de troponine négatif< 99ème percentile. Etude de cohorte prospective bicentrique (CHU Clermont-Ferrand, CH Aurillac) réalisée en aveugle des résultats de la copeptine. Les patients admis aux urgences avec une douleur thoracique de moins de 12h suspecte de suspecte de syndrome coronarien aigu été inclus dans l'étude. Un examen clinique, un électrocardiogramme ainsi que des analyses biologiques ont été réalisés à l'admission, puis à 2, 4, 6 et 12h. La troponine I ultra-sensible a été dosé avec les automates Dimension Vista® (Siemens Healthcare Diagnostic) et la copeptine ultra-sensible avec le KRYPTOR Compact PLUS système (B.R.A.H.M.S). Entre mars 2011 et mars 2012, 102 patients ont été analysés. Notre population se composait de 7,8% de patients avec un syndrome coronarien aigu non ST+ (SCANST+), 3,9% avec un angor instable, 8,9% avec une douleur cardiaque non coronarienne, 52% avec une douleur non cardiaque et 27,4% avec une origine indéterminée de la douleur thoracique. Aucune différence significative n'a été mise en évidence entre les patients avec un SCANST+ et les autres étiologies concernant les taux de copeptine. A l'admission 7 patients avec un SCANST+ et une troponine < 99ème percentile sur 8 avaient également une copeptine négative et se seraient vu exclu du diagnostic d'infarctus du myocarde. Nos résultats permettent de conclure que la copeptine ultrasensible, en association avec la troponine ultrasensible, ne permet pas d'exclure un syndrome coronarien aigu sans sus décalage du segment ST, chez les pateints se présentant aux urgences pour douleur thoracique.CLERMONT FD-BCIU-Santé (631132104) / SudocSudocFranceF

Sperm cryostorage in a dry tank: An accurate alternative

International audienceThis prospective study aimed to determine the effects of dry nitrogen cryostorage on human sperm characteristics in comparison with liquid nitrogen cryostorage. For this purpose, 42 men undergoing routine semen analysis (21 normozoospermia and 21 with altered semen parameters) were analyzed. After slow freezing, half of the straws of each sample were randomly stored in liquid and dry tanks, at the top and bottom levels of the latter. After 6 months storage, thawed samples were treated by density gradient centrifugation and sperm characteristics were compared. There was no difference in sperm progressive motility (15.1% ± 14.2% vs. 15.1% ± 12.7%; p = 0.76), sperm vitality (25.5% ± 17.7% vs. 26.2% ± 19%; p = 0.71), percentages of acrosome-reacted spermatozoa (38% ± 8.5% vs. 38.5% ± 7.4%; p = 0.53) and DNA fragmentation spermatozoa (27.3% ± 12.4% vs. 28.5% ± 12.9%, p = 0.47) after cryostorage in the dry or the liquid nitrogen tank. Moreover, we did not observe differences between either cryostorage system for normal and altered sperm samples. This lack of difference was also observed whatever the floor level of cryostorage in the dry tank. The temperature measurement of the dry tank showed a stable temperature at −194 °C throughout storage whatever the storage floor level, guaranteeing the stability of the low temperatures suitable for human sperm storage. Because of its greater safety, dry storage without contact with the liquid phase should be preferred and can be a useful alternative for the cryostorage of human sperm samples

IVM before vitrification induces alterations of maturation rates and deleterious effects on actin and tubulin architecture of human oocytes. 2018).

International audienc

Fertility preservation and sperm donation in transgender individuals: The current situation within the French CECOS network

International audienc

COVID‐19 lockdown consequences on body mass index and perceived fragility related to physical activity: A worldwide cohort study

International audienceBackground: This paper is a follow-up study continuing the COVISTRESS network previous research regarding health-related determinants.Objective: The aim was to identify the main consequences of COVID-19 lockdown on Body Mass Index and Perceived Fragility, related to Physical Activity (PA), for different categories of populations, worldwide.Design: The study design included an online survey, during the first wave of COVID-19 lockdown, across different world regions.Setting and participants: The research was carried out on 10 121 participants from 67 countries. The recruitment of participants was achieved using snowball sampling techniques via social networks, with no exclusion criteria other than social media access.Main outcome measures: Body Mass Index, Physical Activity, Perceived Fragility and risk of getting infected items were analysed. SPSS software, v20, was used. Significance was set at P < .05.Results: Body Mass Index significantly increased during lockdown. For youth and young adults (18-35 years), PA decreased by 31.25%, for adults (36-65 years) by 26.05% and for the elderly (over 65 years) by 30.27%. There was a high level of Perceived Fragility and risk of getting infected for female participants and the elderly. Correlations between BMI, Perceived Fragility and PA were identified.Discussion and conclusions: The research results extend and confirm evidence that the elderly are more likely to be at risk, by experiencing weight gain, physical inactivity and enhanced Perceived Fragility. As a consequence, populations need to counteract the constraints imposed by the lockdown by being physically active

The major worldwide stress of healthcare professionals during the first wave of the COVID-19 pandemic - the international COVISTRESS survey

IntroductionThe COVID-19 pandemic has initiated an upheaval in society and has been the cause of considerable stress during this period. Healthcare professionals have been on the front line during this health crisis, particularly paramedical staff. The aim of this study was to assess the high level of stress of healthcare workers during the first wave of the pandemic.Materials and methodsThe COVISTRESS international study is a questionnaire disseminated online collecting demographic and stress-related data over the globe, during the pandemic. Stress levels were evaluated using non-calibrated visual analog scale, from 0 (no stress) to 100 (maximal stress).ResultsAmong the 13,537 individuals from 44 countries who completed the survey from January to June 2020, we included 10,051 workers (including 1379 healthcare workers, 631 medical doctors and 748 paramedical staff). The stress levels during the first wave of the pandemic were 57.8 33 in the whole cohort, 65.3 +/- 29.1 in medical doctors, and 73.6 +/- 27.7 in paramedical staff. Healthcare professionals and especially paramedical staff had the highest levels of stress (p &lt; 0.001 vs non-healthcare workers). Across all occupational categories, women had systematically significantly higher levels of work-related stress than men (p &lt; 0.001). There was a negative correlation between age and stress level (r = -0.098, p &lt; 0.001). Healthcare professionals demonstrated an increased risk of very-high stress levels (&gt;80) compared to other workers (OR = 2.13, 95% CI 1.87-2.41). Paramedical staff risk for very-high levels of stress was higher than doctors' (1.88, 1.50-2.34). The risk of high levels of stress also increased in women (1.83, 1.61-2.09; p &lt; 0.001 vs. men) and in people aged &lt;50 (1.45, 1.26-1.66; p &lt; 0.001 vs. aged &gt;50).ConclusionsThe first wave of the pandemic was a major stressful event for healthcare workers, especially paramedical staff. Among individuals, women were the most at risk while age was a protective factor

Changes in Cannabis Consumption During the Global COVID-19 Lockdown: The International COVISTRESS Study

Introduction: COVID-19 lockdown measures have been sources of both potential stress and possible psychological and addiction complications. A lack of activity and isolation during lockdown are among the factors thought to be behind the growth in the use of psychoactive substances and worsening addictive behaviors. Previous studies on the pandemic have attested to an increase in alcohol consumption during lockdowns. Likewise, data suggest there has also been a rise in the use of cannabis, although it is unclear how this is affected by external factors. Our study used quantitative data collected from an international population to evaluate changes in cannabis consumption during the lockdown period between March and October, 2020. We also compared users and non-users of the drug in relation to: (1) socio-demographic differences, (2) emotional experiences, and (3) the information available and the degree of approval of lockdown measures. Methods: An online self-report questionnaire concerning the lockdown was widely disseminated around the globe. Data was collected on sociodemographics and how the rules imposed had influenced the use of cannabis and concerns about health, the economic impact of the measures and the approach taken by government(s). Results: One hundred eighty two respondents consumed cannabis before the lockdown vs. 199 thereafter. The mean cannabis consumption fell from 13 joints per week pre-lockdown to 9.75 after it (p < 0.001). Forty-nine respondents stopped using cannabis at all and 66 admitted to starting to do so. The cannabis users were: less satisfied with government measures; less worried about their health; more concerned about the impact of COVID-19 on the economy and their career; and more frightened of becoming infected in public areas. The risk factors for cannabis use were: age (OR = 0.96); concern for physical health (OR = 0.98); tobacco (OR = 1.1) and alcohol consumption during lockdown (OR = 1.1); the pre-lockdown anger level (OR = 1.01); and feelings of boredom during the restrictions (OR = 1.1). Conclusion: In a specific sub-population, the COVID-19 lockdown brought about either an end to the consumption of cannabis or new use of the drug. The main risk factors for cannabis use were: a lower age, co-addictions and high levels of emotions.publishedVersio

Impact of Yeast Strain on Ester Levels and Fruity Aroma Persistence during Aging of Bordeaux Red Wines

The impact of yeast and lactic acid bacteria strains on the fruity aroma of red wines was investigated by sensory and analytical strategies. The ester composition of four different Bordeaux red wines was quantified by HS-SPME-GC/MS. These wines, made with selected yeast and bacteria strains, were investigated at the end of alcoholic fermentation and regularly until 12 months of aging, during 2011 and 2012 vintages. Sensory analyses of wines after 3 and 12 months of aging revealed significant differences with regard to yeast strains. Bacteria seemed to have only a slight impact on changes in aromatic profile. Ester levels were strongly influenced by yeast strain and very little affected by malolactic fermentation and aging. Differences and similarities between sensory data and ester profile are discussed. This study highlights the importance of yeast strains in red winemaking. Their sensory impact remains despite the other vinification steps after alcoholic fermentation