A study on awareness, occupational risk perception & level of vaccination against hepatitis-B among medical & nursing students in tertiary care hospital, Hyderabad

Background: HBV(Hepatitis B Virus) infection is an occupational hazard for health care workers  and the risk of acquiring HBV infections depends on the frequency of percutaneous and permucosal exposure to blood or blood-contaminated body fluids. Avoiding occupational blood exposure is primary preventive means for the transmission of HBV. However, the single most effective measure for the prevention of hepatitis B is active immunisation.Methods: A cross-sectional study was carried among 204 medical and nursing students in second year of their course using pre-designed questionnaire comprised of questions which included age, sex, qualification, awareness, occupational risk perception & vaccination against hepatitis B infection.  Results: 69.12% of the respondents knew various modes of transmission of hepatitis B. More than half (56.86%) of the respondents knew that HBV infection causes liver cancer. 79.41% of the respondents knew the correct course of action after a needle stick injury. Only 61.27% of the respondents knew that blood soaked cotton and dressings are discarded in yellow coloured bags and that sharps and needles are disposed in white coloured bags. Only 36.46% of medical students & 57.41% of nursing students were completely vaccinated.  Conclusion: Considering the long-term consequences of HBV infection, the health of the study population is at risk. Preventive strategies against the diseases, especially vaccination programmes, should be developed and taken aggressively to improve the vaccination coverage among the study population.

INCREASE THE EFFICIENCY IN WIND TURBINE SYSTEM BY USING DFIG

The problem of fault estimation victimization adjective fault identification observer technique for a DFIG based mostly turbine system. This adjective fault estimation algorithmic program is planned to boost the quickness and accuracy performance of fault estimation. Tn particular, associate degree electrical fault situation, the DFTG winding short circuit fault, is taken into account as a result of its high prevalence rates. supported the fault estimation data, a fault compensator is intended supported fault data provided by the fault identification theme to ensure the stability of the system, and it incorporates with a traditional controller to supply an internet fault compensation of winding contact faults. Finally, the implementation of the planned approach and therefore the results obtained from its application to the DFTG based mostly wind turbine system square measure bestowed parenthetically the potency of the planned methodology

Face Recognition Using the Eigen Face Algorithm to Support Smart Voting

A new authentication technique in online voting system using facial recognition of the voter is used.  In India, currently there are two types of voting system in practice. They are secret Ballet paper and Electronic Voting Machines (EVM), but both of the processes have some limitation or demerits.  In India online voting has not been yet implemented. The current voting system is not safe and secure too. The voters need to go to distributed places like polling booths and stand in a long queue to cast their vote, because of these reasons most of the people misses their chance of voting. The voter who is not eligible can also cast its vote by fake means which may leads to many problems. That’s why in this project we have to propose a system or way for voting which is very effective or useful in voting. In our approach we have three level of security in voting process. The first level is the verification of unique id number (UID), second level is the verification of election id number (EID) and third level is face recognition or face matching. The security level of our system is greatly improved by the new application method for each voter. The user authentication process of the system is improved by adding face recognition in an application which will identify whether the particular user is authenticated user or not

Short-Term History Based Authentication Using Smartphone Sensors and Apps

Secrete-QA could be a security primarily based application. A security question is asked once the user fails to login or forgets his/her password and to reset the password. A security question could be a secondary authentication, but these queries is guessed simply by an admirer or exposed to a strangers who might recognize our personal info like friends, relations or those that will access our personal info through public on-line tools. We will build these security queries additional customized by using the privilege of accessing our Smartphone sensors and apps while not affecting the user’s privacy. Three types of questions are generated that is, Yes/No questions, Multiple Choice questions and WH questions. An android app is created for the frequent updating of the Smartphone data and questions are asked in the web part using this updated data.The questions are generated by considering the legacy apps, GPS, calendar, app details etc.These have the mostmemorability to the users and high robustness to attacks

Corneal Alternations Induced by Topical Application of Benzalkonium Chloride in Rabbit

Benzalkonium chloride (BAC) is the most common preservative in ophthalmic preparations. Here, we investigated the corneal alternations in rabbits following exposure to BAC. Twenty-four adult male New Zealand albino rabbits were randomly divided into three groups. BAC at 0.01%, 0.05%, or 0.1% was applied twice daily to one eye each of rabbits for 4 days. The contralateral untreated eyes were used as control. Aqueous tear production and fluorescein staining scores of BAC-treated eyes were compared with those of controls. The structure of the central cornea was examined by in vivo confocal microscopy. Expression of mucin-5 subtype AC (MUC5AC) in conjunctiva was detected by immunostainig on cryosections. Corneal barrier function was assessed in terms of permeability to carboxy fluorescein (CF). The distribution and expression of ZO-1, a known marker of tight junction, and reorganization of the perijunctional actomyosin ring (PAMR) were examined by immunofluorescence analysis. Although there were no significant differences between control and BAC-treated eyes in Schirmer scores, corneal fluorescein scores and the number of conjunctival MUC5AC staining cells, in vivo confocal microscopy revealed significant epithelial and stromal defects in all BAC-treated corneas. Moreover, BAC at 0.1% resulted in significant increases in central corneal thickness and endothelial CF permeability, compared with those in control eyes, and endothelial cell damage with dislocation of ZO-1 and disruption of PAMR. Topical application of BAC can quickly impair the whole cornea without occurrence of dry eye. A high concentration of BAC breaks down the barrier integrity of corneal endothelium, concomitant with the disruption of PAMR and remodeling of apical junctional complex in vivo

Perfluorocarbon Particle Size Influences Magnetic Resonance Signal and Immunological Properties of Dendritic Cells

The development of cellular tracking by fluorine (19F) magnetic resonance imaging (MRI) has introduced a number of advantages for following immune cell therapies in vivo. These include improved signal selectivity and a possibility to correlate cells labeled with fluorine-rich particles with conventional anatomic proton (1H) imaging. While the optimization of the cellular labeling method is clearly important, the impact of labeling on cellular dynamics should be kept in mind. We show by 19F MR spectroscopy (MRS) that the efficiency in labeling cells of the murine immune system (dendritic cells) by perfluoro-15-crown-5-ether (PFCE) particles increases with increasing particle size (560>365>245>130 nm). Dendritic cells (DC) are professional antigen presenting cells and with respect to impact of PFCE particles on DC function, we observed that markers of maturation for these cells (CD80, CD86) were also significantly elevated following labeling with larger PFCE particles (560 nm). When labeled with these larger particles that also gave an optimal signal in MRS, DC presented whole antigen more robustly to CD8+ T cells than control cells. Our data suggest that increasing particle size is one important feature for optimizing cell labeling by PFCE particles, but may also present possible pitfalls such as alteration of the immunological status of these cells. Therefore depending on the clinical scenario in which the 19F-labeled cellular vaccines will be applied (cancer, autoimmune disease, transplantation), it will be interesting to monitor the fate of these cells in vivo in the relevant preclinical mouse models

Immunolocalisation of P2Y receptors in the rat eye

Nucleotides present an important role in ocular physiology which has been demonstrated by recent works that indicate their involvement in many ocular processes. P2Y are important among P2 receptors since they can control tear production, corneal wound healing, aqueous humour dynamics and retinal physiology. Commercial antibodies have allowed us to investigate the distribution of P2Y receptors in the cornea, anterior and posterior chamber of the eye and retina. The P2Y1 receptor was present mainly in cornea, ciliary processes, and trabecular meshwork. The P2Y2 receptors were present in cornea, ciliary processes and retinal pigmented epithelium. P2Y4 was present in cornea, ciliary processes, photoreceptors, outer plexiform layer and ganglion cell layer. The P2Y6 presented almost an identical distribution as the P2Y4 receptor. The P2Y11 was also detectable in the retinal pigmented epithelium. The detailed distribution of the receptors clearly supports the recent findings indicating the relevant role of nucleotides in the ocular function

Synthesis and characterization of BaTiO3/-Fe2O3 core/shell structure

Multiferroic materials attracted a lot of attention in recent years because of their significant scientific interest and technological applications. The multiferroic core/shell powders have a better connectivity between the phases, resulting in superior dielectric and magneto electric properties. In this study, the influence of preparation condition on structure and properties of BaTiO3/-Fe2O3 core/shell composite materials was examined. The five samples were obtained by varying synthesis conditions, such as synthesized method (co-precipitation and sonochemical method) and pH values of solution. XRD and Raman spectroscopy analyses were performed in order to determine phase composition and structural changes within samples. Morphology modifications were examined by SEM and EDS analyses. Finally, effect of structural and microstructural changes on magnetic and electrical properties was detected and explained

Time-Lapse Analysis and Mathematical Characterization Elucidate Novel Mechanisms Underlying Muscle Morphogenesis

Skeletal muscle morphogenesis transforms short muscle precursor cells into long, multinucleate myotubes that anchor to tendons via the myotendinous junction (MTJ). In vertebrates, a great deal is known about muscle specification as well as how somitic cells, as a cohort, generate the early myotome. However, the cellular mechanisms that generate long muscle fibers from short cells and the molecular factors that limit elongation are unknown. We show that zebrafish fast muscle fiber morphogenesis consists of three discrete phases: short precursor cells, intercalation/elongation, and boundary capture/myotube formation. In the first phase, cells exhibit randomly directed protrusive activity. The second phase, intercalation/elongation, proceeds via a two-step process: protrusion extension and filling. This repetition of protrusion extension and filling continues until both the anterior and posterior ends of the muscle fiber reach the MTJ. Finally, both ends of the muscle fiber anchor to the MTJ (boundary capture) and undergo further morphogenetic changes as they adopt the stereotypical, cylindrical shape of myotubes. We find that the basement membrane protein laminin is required for efficient elongation, proper fiber orientation, and boundary capture. These early muscle defects in the absence of either lamininβ1 or lamininγ1 contrast with later dystrophic phenotypes in lamininα2 mutant embryos, indicating discrete roles for different laminin chains during early muscle development. Surprisingly, genetic mosaic analysis suggests that boundary capture is a cell-autonomous phenomenon. Taken together, our results define three phases of muscle fiber morphogenesis and show that the critical second phase of elongation proceeds by a repetitive process of protrusion extension and protrusion filling. Furthermore, we show that laminin is a novel and critical molecular cue mediating fiber orientation and limiting muscle cell length

Polymorphisms in the hypoxia-inducible factor 1 alpha gene in Mexican patients with preeclampsia: A case-control study

<p>Abstract</p> <p>Background</p> <p>Although the etiology of preeclampsia is still unclear, recent work suggests that changes in circulating angiogenic factors play a key role in its pathogenesis. In the trophoblast of women with preeclampsia, hypoxia-inducible factor 1 alpha (HIF-1α) is over-expressed, and induces the expression of non-angiogenic factors and inhibitors of trophoblast differentiation. This observation prompted the study of HIF-1α and its relation to preeclampsia. It has been described that the C1772T (P582S) and G1790A (A588T) polymorphisms of the <it>HIF1A </it>gene have significantly greater transcriptional activity, correlated with an increased expression of their proteins, than the wild-type sequence. In this work, we studied whether either or both <it>HIF1A </it>variants contribute to preeclampsia susceptibility.</p> <p>Results</p> <p>Genomic DNA was isolated from 150 preeclamptic and 105 healthy pregnant women. Exon 12 of the <it>HIF1A </it>gene was amplified by PCR, and the genotypes of <it>HIF1A </it>were determined by DNA sequencing.</p> <p>In preeclamptic women and controls, the frequencies of the T allele for C1772T were 4.3 vs. 4.8%, and the frequencies of the A allele for G1790A were 0.0 vs. 0.5%, respectively. No significant differences were found between groups.</p> <p>Conclusion</p> <p>The frequency of the C1772T and G1790A polymorphisms of the <it>HIF1A </it>gene is very low, and neither polymorphism is associated with the development of preeclampsia in the Mexican population.</p