Techniques for determination of impact forces during walking and running in a zero-G environment

One of the deleterious adaptations to the microgravity conditions of space flight is the loss of bone mineral content. This loss appears to be at least partially attributable to the minimal skeletal axial loading concomitant with microgravity. The purpose of this study was to develop and fabricate the instruments and hardware necessary to quantify the vertical impact forces (Fz) imparted to users of the space shuttle passive treadmill during human locomotion in a three-dimensional zero-gravity environment. The shuttle treadmill was instrumented using a Kistler forceplate to measure vertical impact forces. To verify that the instruments and hardware were functional, they were tested both in the one-G environment and aboard the KC-135 reduced gravity aircraft. The magnitude of the impact loads generated in one-G on the shuttle treadmill for walking at 0.9 m/sec and running at 1.6 and 2.2 m/sec were 1.1, 1.7, and 1.7 G, respectively, compared with loads of 0.95, 1.2, and 1.5 G in the zero-G environment

Visfatin reduces gap junction mediated cell-to-cell communication in proximal tubule-derived epithelial cells

Background/Aims: In the current study we examined if the adipocytokine, visfatin, alters connexin-mediated intercellular communication in proximal tubule-derived epithelial cells. Methods: The effects of visfatin (10-200ng/mL) on cell viability and cytotoxicity in HK2-cells were assessed by MTT, crystal violet and lactate dehydrogenase assays. Western blot analysis was used to confirm expression of Cx26, Cx40 and Cx43. The effect of visfatin (10-200ng/mL) on TGF-β1 secretion was confirmed by ELISA, and the effects of both TGF-β1 (2-10ng/mL) and visfatin (10-200ng/mL) on connexin expression were assessed by western blot. Functional intercellular communication was determined using transfer of Lucifer Yellow and paired-whole cell patch clamp electrophysiology. Results: In low glucose (5mM), visfatin (10-200ng/mL) did not affect membrane integrity, cytotoxicity or cell viability at 48hrs, but did evoke a concentration-dependent reduction in Cx26 and Cx43 expression. The expression of Cx40 was unaffected. At 48hrs, visfatin (10-200ng/mL) increased the secretion of TGF-β1 and the visfatin-evoked changes in connexin expression were mimicked by exogenous application of the pro-fibrotic cytokine (2-10ng/ml). Visfatin reduced dye transfer between coupled cells and decreased functional conductance, with levels falling by 63% as compared to control. Although input resistance was increased following visfatin treatment by 166%, the change was not significant as compared to control. The effects of visfatin on Cx-expression and cell-coupling were blocked in the presence of a TGF-β1 specific neutralizing antibody. Conclusions: The adipocytokine visfatin selectively evoked a non-toxic reduction in connexin expression in HK2-cells. The loss in gap-junction associated proteins was mirrored by a loss in functional conductance between coupled cells. Visfatin increased TGF-β secretion and the pattern of change for connexins expression was mimicked by exogenous application of TGF-β1. The effect of visfatin on Cx-expression and dye transfer were negated in the presence of a TGF-β1 neutralising antibody. These data suggest that visfatin reduces connexin-mediated intercellular communication in proximal tubule-derived epithelial cells via a TGF-β dependent pathway. © 2013 S. Karger AG, Base

SARS-CoV-2 update from the World Small Animal Veterinary Association

Priorities in veterinary preventive healthcare for dogs and cats under COVID-19 pandemic restrictions: -Routine prophylactic vaccines -Parasite control measures -Other preventive or health monitoring measure

Regional spillover of housing (un)affordability: an empirical study on the residential housing markets for first-time buyers in the U.K.

This study examines the lead–lag relationships between the levels of housing affordability of different regions of the U.K. By utilizing government data, a number of housing affordability indicators are constructed to explore whether spatial diffusion exists between different regional submarkets of first-time homebuyers over the period of 2000 – 2021. The results reveal that during periods of economic expansion, housing unaffordability tended to diffuse from regions of slower economic growth to regions of higher economic development. It is further evident that in the aftermath of the GFC, the London housing market Granger-caused other regional markets in terms of housing (un)affordability. Lastly, the U.K’.s decision to leave the EU in 2016 seems to have led to more divergence between the submarket regions, which in a Granger causal sense, have become less causally correlated in terms of pricing. We conjecture that the causal interactions between the different regional housing submarkets exist with the lead–lag relationships governed primarily by their underlying macroeconomic fundamentals

A method of evaluating efficiency during space-suited work in a neutral buoyancy environment

The purpose was to investigate efficiency as related to the work transmission and the metabolic cost of various extravehicular activity (EVA) tasks during simulated microgravity (whole body water immersion) using three space suits. Two new prototype space station suits, AX-5 and MKIII, are pressurized at 57.2 kPa and were tested concurrently with the operationally used 29.6 kPa shuttle suit. Four male astronauts were asked to perform a fatigue trial on four upper extremity exercises during which metabolic rate and work output were measured and efficiency was calculated in each suit. The activities were selected to simulate actual EVA tasks. The test article was an underwater dynamometry system to which the astronauts were secured by foot restraints. All metabolic data was acquired, calculated, and stored using a computerized indirect calorimetry system connected to the suit ventilation/gas supply control console. During the efficiency testing, steady state metabolic rate could be evaluated as well as work transmitted to the dynamometer. Mechanical efficiency could then be calculated for each astronaut in each suit performing each movement

There is another way: The faculty-developed Idaho Comprehensive Literacy Assessment for K-8 pre-service teachers

In this era of external teacher testing with the intent of ensuring the competence of the teaching force, as well as holding students and institutions accountable for results, the Idaho Comprehensive Literacy Assessment (ICLA) stands in sharp contrast. It represents an alternative to external testing of preservice teachers, testing procured from an outside agency unconnected to preservice programs, since it has been developed and is managed by literacy faculty from Idaho’s major institutions of higher learning. This paper provides a brief history of major events in the field of literacy including teacher testing initiatives and policies, which led to the creation of the ICLA. A description of the ICLA assessment and its construction is provided, along with a report of initial performance. Implications and policy consequences of this approach are explored

Predictors and Correlates of Psychological Health Through Ecological Momentary Assessment

Ecological momentary assessment (EMA) entails taking surveys multiple times daily on a smartphone. EMA has been widely used to monitor moment-to-moment fluctuations in thoughts, feelings, and behaviors as they occur naturally in one\u27s environment. The current study investigated how depression, productivity, and sleep, fluctuate across time and how these variables covary together in undergraduate participants using EMA for two weeks. Participants were either assigned to EMA which included items about mindfulness (Mindfulness-EMA) or without mindfulness items (No-Mindfulness EMA). Participants were asked to report upon current depression, stress, irritability, productivity, fatigue, and sleep, up to three times daily. Multilevel modeling was used to examine trajectories of change and how variables may increase or decrease over the span of two weeks. The sample (n=103) included 60.9% female, 37% non-white, and 11% Latino/Hispanic participants, with a mean age of 19 (SD = 1.24). Nearly half (47.8%) were freshmen. Preliminary analyses reveal a significant time effect for stress (p=.000); anxiety (p=.001); irritability (p=.002); and sleep disturbances (p=.004), such that all variables improved over two weeks for all participants. Fatigue, depression, and dissociation did not significantly change. Subsequent analyses will examine how trajectories of change covary by demographics, such as sex and year in college.https://orb.binghamton.edu/research_days_posters_spring2020/1085/thumbnail.jp