A Review of Central Asian Glaciochemical Data

The glaciers of central Asia provide suitable locations from which to recover continuous, high-resolution glaciochemical records on a continental scale. Although the glaciochemical investigations undertaken to date in central Asia are few in number and limited in terms of spatial coverage and length of record, some preliminary observations can be made concerning regional and seasonal trends in snow chemistry in this region. The sodium chloride ratio for most snow samples collected in central Asia approaches the ratio found in sea water (0.86 in μeq kg-1), reflecting a marine source for these constituents. Sodium and chloride concentrations are, on average, 3-10 times higher in the Himalayas than in the Karakoram, demonstrating the greater influence of monsoonal sources of moisture in the Himalayas. Very high sodium concentrations from Khel Khod Glacier probably reflect a local crustal source from surrounding ice-free areas. Low nitrate concentrations were found in snow collected from the southern margin of the Himalayas and high concentrations in snow deposited on the north margin of the Himalayas. This strong regional trend in the spatial distribution of nitrate suggests the influx of continental aerosols, rich in nitrate, originating from the arid regions of central Asia. High calcium concentrations measured in snow from Mount Everest and the north-west corner of China are also indicative of dust derived from the arid regions of central Asia. Very high sulfate concentrations found in snow from the Tien Shan and the Bogda Shan most likely reflect local anthropogenic sources. The altitude effect on isotopic composition is not apparent from snow samples collected in central Asia. Understanding the processes which control the chemical content of snow, the local-to-regional scale complexities, and the seasonal variability are fundamental steps necessary to assess the potential for recovering representative long-term glaciochemical records from central Asia

Two-hundred-year Record of Biogenic Sulfur in a South Greenland Ice Core (20D)

The concentration of methanesulfonic acid (MSA) was determined in a shallow south central Greenland ice core(20D). This study provides a high-resolution record of the DMS-derived biogenic sulfur in Greenland precipitation over the past 200 years. The mean concentration of MSA is 3.30 ppb(σ = 2.38 ppb,n = 1134). The general trend of MSA is an increase from 3.01 to 4.10 ppb between 1767 and 1900, followed by a steady decrease to 2.34 ppb at the present time. This trend is in marked contrast to that of non-sea-salt sulfate (nss SO42-), which increases dramatically after 1900 due to the input of anthropogenic sulfur. The MSA fraction ((MSA/(MSA+ nss SO42-))* 100) ranges from a mean of 15% in preindustrial ice to less than 5% in recent ice. These MSA fraction suggest that approximately 5 to 40% of the sulfur in recent Greenland ice is of biological origin. It is suggested that there is a significant low-latitude component to the biogenic sulfur in the core and that variations in the MSA fraction reflect changes in the relative strengths of low- and high-latitude inputs. The data shown o evidence for a strong dependence of dimethyl sulfide(DMS) emissions on sea surface temperature during the last century. There is also no indication that the yield of MSA from DMS oxidation has been altered by increased NOx levels over the North Atlantic during this period

Impaired Regulation Post-stroke of Motor Unit Firing Behavior during Volitional Relaxation of Knee Extensor Torque Assessed Using High Density Surface EMG Decomposition

The purpose of this study was to use high density surface EMG recordings to quantify stroke-related abnormalities in motor unit firing behavior during repeated sub-maximal knee extensor contractions. A high density surface EMG system (sEMG) was used to record and extract single motor unit firing behavior in the vastus lateralis muscle of 6 individuals with chronic stroke and 8 controls during repeated sub-maximal isometric knee extension contractions. Paretic motor unit firing rates were increased with subsequent contractions (6.19±0.35 pps vs 7.89±0.66 pps,

A 1400-Year Oxygen Isotope History from the Ross Sea Area, Antarctica

Four ice cores from the Ross Sea drainage, Antarctica, show patterns of δ18O variations on a time scale of decades to centuries over the last 1400 years without change in the long-term average δ18O. Century scale δ18O fluctuations in the two cores drilled in the Ross Ice Shelf at Station J-9 (82° 23\u27 S, 168° 38\u27 W, elevation 60 m) are highly correlated (P \u3c 2 x 10-4). The long isotope record (\u3e30 000 a) of the 1978 1-9 core thus represents local conditions over at least 102 m and on time scales of 100 years and longer. Regional correlations between the 1-9 δ18O records and those from Ridge BC (82 ° 54\u27S, 136 ° 40\u27W, elevation 509 m) and the Dominion Range (85 ° 15\u27 S, 166 ° 10\u27 E, elevation 2700 m) are barely significant (P ≈ 0.05 for J-9 \u2776 and Dominion Range, δ18O to 1400 years ago) or absent. The failure to find clear regional isotope trends related to climate fluctuations may reflect the finding that between 1957 and 1982 the area was in the transition zone between areas with opposite temperature trends, and showed little or no temperature change. The fact that the records nevertheless show significant δ18O fluctuations highlights the need to base regional climate reconstructions on a regional suite of ice-core records

HMG20B stabilizes association of LSD1 with GFI1 on chromatin to confer transcription repression and leukemia cell differentiation block

Pharmacologic inhibition of LSD1 induces molecular and morphologic differentiation of blast cells in acute myeloid leukemia (AML) patients harboring MLL gene translocations. In addition to its demethylase activity, LSD1 has a critical scaffolding function at genomic sites occupied by the SNAG domain transcription repressor GFI1. Importantly, inhibitors block both enzymatic and scaffolding activities, in the latter case by disrupting the protein:protein interaction of GFI1 with LSD1. To explore the wider consequences of LSD1 inhibition on the LSD1 protein complex we applied mass spectrometry technologies. We discovered that the interaction of the HMG-box protein HMG20B with LSD1 was also disrupted by LSD1 inhibition. Downstream investigations revealed that HMG20B is co-located on chromatin with GFI1 and LSD1 genome-wide; the strongest HMG20B binding co-locates with the strongest GFI1 and LSD1 binding. Functional assays demonstrated that HMG20B depletion induces leukemia cell differentiation and further revealed that HMG20B is required for the transcription repressor activity of GFI1 through stabilizing LSD1 on chromatin at GFI1 binding sites. Interaction of HMG20B with LSD1 is through its coiled-coil domain. Thus, HMG20B is a critical component of the GFI1:LSD1 transcription repressor complex which contributes to leukemia cell differentiation block

Platelet activation in cystic fibrosis

Cystic fibrosis (CF) is caused by a mutation of the gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR). We examined platelet function in CF patients because lung inflammation is part of this disease and platelets contribute to inflammation. CF patients had increased circulating leukocyte-platelet aggregates and increased platelet responsiveness to agonists compared with healthy controls. CF plasma caused activation of normal and CF platelets; however, activation was greater in CF platelets. Furthermore, washed CF platelets also showed increased reactivity to agonists. CF platelet hyperreactivity was incompletely inhibited by prostaglandin E(1) (PGE(1)). As demonstrated by Western blotting and reverse-transcriptase-polymerase chain reaction (RT-PCR), there was neither CFTR nor CFTR-specific mRNA in normal platelets. There were abnormalities in the fatty acid composition of membrane fractions of CF platelets. In summary, CF patients have an increase in circulating activated platelets and platelet reactivity, as determined by monocyte-platelet aggregation, neutrophil-platelet aggregation, and platelet surface P-selectin. This increased platelet activation in CF is the result of both a plasma factor(s) and an intrinsic platelet mechanism via cyclic adenosine monophosphate (cAMP)/adenylate cyclase, but not via platelet CFTR. Our findings may account, at least in part, for the beneficial effects of ibuprofen in CF

Seven 3-methylidene-1H-indol-2(3H)-ones related to the multiple-receptor tyrosine kinase inhibitor sunitinib

The solid-state structures of a series of seven substituted 3-methylidene-1H-indol-2(3H)-one derivatives have been determined by single-crystal X-ray diffraction and are compared in detail. Six of the structures {(3Z)-3-(1H-pyrrol-2- ylmethylidene)-1H-indol-2(3H)-one, C13H10N2O, (2a); (3Z)-3-( 2-thienylmethylidene)-1H-indol-2(3H)-one, C13H9NOS, (2b); (3E)-3-(2-furylmethylidene)-1H-indol-2(3H)-one monohydrate, C13H9NO2 center dot H2O, (3a); 3-(1-methylethylidene)-1H-indol- 2(3H)-one, C11H11NO, (4a); 3-cyclohexylidene-1H-indol- 2(3H)-one, C14H15NO, (4c); and spiro[1,3-dioxane-2,3'-indolin]- 2'-one, C11H11NO3, (5)} display, as expected, intermolecular hydrogen bonding (N-H center dot center dot center dot O=C) between the 1H-indol-2(3H)-one units. However, methyl 3-(1-methylethylidene)- 2-oxo-2,3-dihydro-1H-indole-1-carboxylate, C13H13NO3, (4b), a carbamate analogue of (4a) lacking an N-H bond, displays no intermolecular hydrogen bonding. The structure of (4a) contains three molecules in the asymmetric unit, while (4b) and (4c) both contain two independent molecules