BACTÉRIAS PROMOTORAS DO CRESCIMENTO RADICULAR EM PLÂNTULAS DE DOIS CULTIVARES DE ARROZ IRRIGADO POR INUNDAÇÃO

For growing and development the plants, in nature, count with the benefits of microorganisms residing in their rhizosphere. Plant growth promoting rhizobacteria (PGPR) are some of these microorganisms that interact in a beneficial way with plant species. Therefore, seed inoculation with PGPR can be an important and sustainable alternative to improve initial development and crop production. The aim of this study was to determine the effect of seeds microbiolization with PGPR on the root length of lowland rice seedlings. Under controlled conditions, two experiments were conducted with lowland rice cultivars (BRS Catiana and A 702 CL) in completely randomized design, with seven treatments and 10 replications. The treatments consisted of six microorganisms: BRM32109 (Bacillus sp.), BRM32110 (Bacillus sp.), BRM32111 (Pseudomonas sp.), BRM32112 (Pseudomonas fluorescens), BRM32113 (Burkholderia pyrrocinia), BRM32114 (Serratia sp.) and a control (without microorganism). The data were submitted to analysis of variance, the means among the treatments with PGPR were compared by LSD test (p<0.05) and, the treatments with microorganisms were individually compared to the control by Dunnett test (p<0.05). Only the cultivar BRS Catiana showed difference among the treatments. The highest root length was presented by the seeds treated with the isolates BRM32110, BRM32112, BRM32109, and BRM32114. Both of cultivars had significance when compared microorganisms to the control. Highlight for seeds of cultivar BRS Catiana, that when treated with the isolates BRM32110 and BRM32112 presented an average increase of 25.7% on the root length, compared to control treatment. For the cultivar A 702 CL, all the microorganisms provided increments with average value of 31% in the root length of rice seedlings. Based on the results it can be inferred that PGPR provide significant increases in seedling root length of lowland rice cultivars.Para o crescimento e desenvolvimento as plantas, na natureza, contam com os benefícios de microrganismos residentes em sua rizosfera. Rizobactérias promotoras de crescimento vegetal (RPCV) são exemplos desses microrganismos que interagem de forma benéfica com espécies vegetais. Portanto, a inoculação de sementes com RPCV pode representar uma alternativa importante e sustentável para melhorar o desenvolvimento inicial e a produção de plantas cultivadas. Objetivou-se com esse estudo determinar o efeito da microbiolização de sementes com RPCV no comprimento radicular de plântulas de arroz irrigado. Em condições controladas, conduziram-se dois experimentos com cultivares de arroz irrigado (BRS Catiana e A 702 CL), em delineamento inteiramente casualizado, com sete tratamentos e 10 repetições. Os tratamentos constituíram-se de seis microrganismos: BRM32109 (Bacillus sp.), BRM32110 (Bacillus sp.), BRM32111 (Pseudomonas sp.), BRM32112 (Pseudomonas fluorescens), BRM32113 (Burkholderia pyrrocinia), BRM32114 (Serratia sp.) e um controle (sem microrganismo). Os dados foram submetidos à análise de variância, as médias entre os tratamentos com RPCV foram comparadas pelo teste LSD (p≤0,05) e, os tratamentos com microrganismos foram comparados individualmente com o controle pelo teste de Dunnett (p≤0,05). Somente a cultivar BRS Catiana apresentou diferença entre os tratamentos. Os maiores comprimentos radiculares foram apresentados pelas sementes tratadas com os isolados BRM32110, BRM32112, BRM32109 e BRM32114. Para ambas cultivares houve significância quando comparado microrganismos e controle. Destaque para as sementes da cultivar BRS Catiana, que quando tratadas com os isolados BRM32110 e BRM32112 apresentaram aumento médio de 25,7% no comprimento radicular, em comparação com o tratamento controle. Para a cultivar A 702 CL, todos os microrganismos proporcionaram incrementos com um valor médio de 31% no comprimento radicular das plântulas de arroz. Com base nos resultados pode-se inferir que as RPCV proporcionam incrementos significativos no comprimento radicular de plântulas de cultivares de arroz irrigado

Angina bullosa haemorrhagica:a 14-year multi-institutional retrospective study from Brazil and literature review

Angina bullosa haemorrhagica (ABH) is characterized by the recurrent appearance of blood blisters on the oral mucosa, mainly in adults' soft palate. In general, the blisters rupture spontaneously, lacking the necessity for biopsy. We report the clinical features of 23 ABH cases, emphasizing the clinical behavior and the management of these conditions. A retrospective descriptive cross-sectional study was performed. A total of 12,727 clinical records of oral and maxillofacial lesions from four dental services in Brazil were analyzed. Clinical data were collected from the clinical records and evaluated. The series comprised 12 males (52.2%) and 11 females (47.8%), with a mean age of 56.8?±?14.6?years (ranging: 24-82?years) and a 1.1:1 male-to-female ratio. Most of the lesions affected the soft palate (n = 15, 65.2%). Clinically, the lesions presented mainly as an asymptomatic (n = 17, 73.9%) blood-filled blister that ruptured after a few minutes or hours, leaving an erosion. The masticatory trauma was the most frequent triggering event. No patient had coagulation disorders. A biopsy was performed in only four cases (17.4%). Treatment was symptomatic with a favorable outcome. ABH is still poorly documented in the literature, and its etiology remains uncertain. ABH mainly affects the soft palate of elderly adults and has a favorable evolution in a few days. The therapeutic approach is often focused only on the relief of symptoms. However, it can share some clinical features with more serious diseases. Therefore, clinicians must recognize these lesions to avoid misdiagnosis

Altura de planta e diâmetro de colmo em cana-de-açúcar de segundo corte fertilizada com organomineral de lodo de esgoto e bioestimulante / Height of plant and thermal diameter in second cut sugar fertilized with organomineral of sewage sludge and bioestimulant

O Brasil é o maior produtor de cana-de-açúcar (Saccharum spp.). O objetivo desse estudo foi avaliar o desenvolvimento da cana-de-açúcar de segundo corte sob adubação organomineral a base de lodo de esgoto e bioestimulante. O experimento foi conduzido em delineamento em blocos casualizados em esquema fatorial 5x2+1. Foram cinco tratamentos com e sem biestimulante, mais um adicional (adubação mineral) em quatro repetições. As combinações dos tratamentos foram em função da recomendação de adubação de plantio de 470 kg ha-1 da formulado 04-21-07 e cobertura de 400 kg ha-1 da formulação 10-00-40 + 0,7% B aos 150 dias após plantio (DAP) e cobertura aos 90 dias após o corte (DAC). Utilizou-se a análise do solo para recomendar as adubações. Os tratamentos consistiram-se: 100 % com fonte mineral; 0; 60; 80; 100 e 120 % (Com e Sem Bioestimulante) da fonte organomineral a base lodo de esgoto. Foram avaliados altura de plantas e diâmetro de colmo. Não houve diferença da fonte organomineral de lodo de esgoto e mineral relacionado com altura e o diâmetro. O uso de bioestimulante também não influencia na altura e diâmetro. 

Primary melanoma of the oral cavity:a multi-institutional retrospective analysis in Brazil

Melanoma is an aggressive malignant tumor, rarely observed in the oral cavity. The aim of this study was to describe the clinicopathologic features of a series of oral melanomas. A retrospective descriptive study was performed. A total of 15,482 biopsy records from two oral and maxillofacial pathology services in Brazil were analyzed. All cases of oral melanomas were reviewed, and clinical, demographic, histopathological data, treatment, and follow-up status were collected. In addition, immunohistochemistry stains (pan-cytokeratin AE1/AE3, vimentin, ?-SMA, CD45, S-100 protein, HMB-45, Melan A, and Ki-67) were performed. The series comprised of 5 males (71.4%) and 2 females (28.6%), with a mean age of 58.0 ± 9.2 years (range: 45-69 years) and a 2.5:1 male-to-female ratio. The gingiva (n = 3, 42.8%) and hard palate (n = 2, 28.6%) were the most common affected sites, presenting clinically as ulcerated swellings with a brown to black color. Cervical lymph node metastasis was detected in three patients during the first examination. Microscopically, 6 cases (85.7%) were melanotic, and one (14.3%) was amelanotic. Most cases (n = 4, 57.1%) presented a predominance of epithelioid cells. S-100 and HMB-45 were positive in all cases (n = 7, 100.0%). In contrast, only 4 cases (57.1%) were positive for Melan-A. The proliferative index with Ki-67 was high, with labeling index ranging from 70.0% to more than 90% of positive cells. Five patients died from complications of the tumors after a mean follow-up period of 7.8 months. Melanoma is an aggressive malignant tumor that rarely occurs in the oral cavity. It occurs mainly in adult and elderly patients and often is diagnosed in advanced stages. The current findings were similar to previous studies and reflected the characteristics of the services from where lesions were retrieved

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality

Trichoderma asperellum AND RHIZOBACTERIA AS AFFECTING BIOMASS ACCUMULATION AND GAS EXCHANGE OF LOWLAND RICE

Rice is a staple food for almost four billion people worldwide. Rice plants treated with multifunctional microorganisms may be a strategic way for sustainable improvement of crop grain yield. This work had the objective to study the effect of types and ways of microorganisms application in tropical lowland rice production, evaluated by biomass production and gas exchange. The greenhouse trial was in a completely randomized design, in factorial scheme 7 x 3 + 1, with four replication. Treatments were the combination of seven microorganisms: Bacillus sp. (BRM32109 e BRM32110); Pseudomonas fluorescens (BRM32111); Pseudomonas sp. (BRM32112); Burkholderia pyrrocinia (BRM32113); Serratia sp. (BRM32114) and Trichoderma asperellum pool (UFRA.T06 + UFRA.T09 + UFRA.T12 + UFRA.T52), with three application ways (1. microbiolized seed; 2. microbiolized seed + soil drenched with microorganism at eight and 15 days after sowing (DAS) and 3. microbiolized seed + microorganism spray plant at eight and 15 DAS). Microbiolized seed was the best microorganism way, which allowed highest instantaneous carboxylation efficiency and, biomass production in lowland rice. Rhizobcteria BRM32114, followed by BRM32111, BRM32112 and fungi T. asperellum allowed, an average, improvement of 17% in instantaneous carboxylation efficiency and 20% in dry shoot biomass production of lowland rice compared to the control plants.Rice the main source of energy to maintain the metabolism for almost four billion people worldwide. Rice plants treated with multifunctional microorganisms may be a good way for sustainable improvement of crop grain yield. Aimed study the effect of types and ways of microorganisms application in tropical lowland rice production, evaluated by biomass production and gas exchange. The greenhouse trial was in a completely randomized design, in factorial scheme 7 x 3 + 1, with four replication. Treatments were the combination of seven microorganisms: Bacillus sp. (BRM32109 e BRM32110); Pseudomonas fluorescens (BRM32111); Pseudomonas sp. (BRM32112); Burkholderia pyrrocinia (BRM32113); Serratia sp. (BRM32114) and Trichoderma asperellum pool (UFRA.T06 + UFRA.T09 + UFRA.T12 + UFRA.T52), with three forms of application (1. microbiolized seed; 2. microbiolized seed + soil drenched with microorganism at eight and 15 days after sowing (DAS) and 3. microbiolized seed + microorganism spray plant at eight and 15 DAS). Microbiolized seed was the best form of application, which allowed highest instantaneous carboxylation efficiency and yield of biomass production in lowland rice. Rhizobcteria BRM32114, followed by BRM32111, BRM32112 and fungi T. asperellum improved, an average, 17% in instantaneous carboxylation efficiency and 20% in dry shoot biomass production of lowland rice compared to the control plants

Momento Científico, Workshop de Habilidade Médica da IFMSA Brasil - Edição Especial

In this special edition of the Scientific Moment, Workshop on Medical Skills of IFMSA Brazil, five abstracts covering various health areas are presented, authored by students, professors, and healthcare professionals from the Federal University of Roraima, General Hospital of Roraima, and the Faculty of Industry of Paraná. The topics include epidemiological analysis of sepsis mortality, polypharmacy in seropositive elderly individuals, new perspectives on autism, breast cancer epidemiology in Roraima, and epidemiological analysis of sepsis mortality.Nesta edição especial do Momento Científico, Workshop de Habilidade Médica da IFMSA Brasil, são apresentados cinco resumos que abordam diversas áreas da saúde, elaborados por estudantes, professores e profissionais da saúde da Universidade Federal de Roraima, Hospital Geral de Roraima e da Faculdade da Indústria do Paraná. Os temas incluem análise epidemiológica da mortalidade por sepse, polifarmácia em idosos soropositivos, novas perspectivas sobre o autismo, epidemiologia do câncer de mama em Roraima e análise epidemiológica da mortalidade por sepse

SARS-CoV-2 in children with cancer in Brazil : results of a multicenter national registry

Background: Strategies to mitigate the impact of COVID-19 in special populations are complex and challenging. Few studies have addressed the impact of COVID-19 on pediatric patients with cancer in low- and middle-income countries. Methods: Multicenter observational cohort study with prospective records and retrospective analyses starting in April 2020 in 21 pediatric oncology centers distributed throughout Brazil. Participants: Patients under 18 years of age who are infected by the SARS-CoV-2 virus (confirmed diagnosis through reverse transcriptase-polymerase chain reaction [RT-PCR]) while under treatment at pediatric oncology centers. The variables of interest included clinical symptoms, diagnostic and therapeutic measures. The repercussions of SARS-CoV-2 infection on cancer treatment and general prognosis were monitored. Results: One hundred seventy-nine patients were included (median age 6 [4–13] years, 58% male). Of these, 55.9% had acute leukemia and 34.1% had solid tumors. The presence of SARS-CoV-2 was diagnosed by RT-PCR. Various laboratory markers were analyzed, but showed no correlation with outcome. Children with low or high BMI for age had lower overall survival (71.4% and 82.6%, respectively) than those with ageappropriate BMI (92.7%) (p = .007). The severity of presentation at diagnosis was significantly associated with outcome (p < .001). Overall mortality in the presence of infection was 12.3% (n = 22). Conclusion: In children with cancer and COVID-19, lower BMI was associated with worse prognosis. The mortality in this group of patients (12.3%) was significantly higher than that described in the pediatric population overall (~1%)