Incorporación de fibra de eucalipto y fécula de papa en impermeabilización y espesor de subrasante del camino Piuray - Ccorimarca, 2022

El presente proyecto tuvo como objetivo determinar cómo la incorporación de fibra de eucalipto y fécula de papa influye en la impermeabilización y espesor de subrasante del camino Piuray Ccorimarca 2022. Como objetivos específicos se plantea: Determinar cómo influye la fibra de eucalipto en la impermeabilización de la subrasante del camino vecinal, Determinar cómo influye la incorporación de fécula de papa en la impermeabilización de la subrasante del camino vecinal, Determinar cómo influye la incorporación de fibra de eucalipto en el” espesor del camino vecinal y cómo influye la incorporación de fécula de papa en el espesor del camino vecinal. El tipo de investigación de Aplicado – Tecnológico, con un nivel explicativo, enfoque cuantitativo y diseño experimental puro. La población corresponde a 110 ensayos distribuidos con la incorporación de fibra de eucalipto y fécula de papa en dosificaciones 2%, 4%, 6%, 8% y 10% respectivamente. La técnica de investigación es la observación directa y el instrumento consta de la utilización de formularios de recolección de datos, equipos, herramientas de laboratorio y software de procesamiento de datos. Los resultados fueron: La incorporación más efectiva es bajo el 2% de fibra de eucalipto, con una permeabilidad de 56.7522 x 10-6 cm/s. La incorporación más efectiva es bajo el 6% de fécula de papa, con una permeabilidad de 5.6256 x 10-6 cm/s. La incorporación de fécula de papa al 10% resulta más profunda, con un espesor de 10.41 cm. Respecto a la fécula de papa, la más efectiva para propósitos de impermeabilización y espesor es de 6

Revista historia de la educación Latinoamericana No 16

Esta publicación se ha dedicado al tema monográficode los textos escolares. La relevancia del tema laasumimos por las investigaciones que sobre estecontenido viene desarrollando un equipo desde Franciay España. Esta red presenta vínculos con investigadores,en América Latina, donde podemos destacar enVenezuela al Dr. José Pascual Mora, presidente de laSociedad de Historia de la Educación Latinoamericana.Pero hay que decir que principalmente, se motivó eltema monográfico, por la investigación que realizó elGrupo de investigación: “Historia y Prospectiva de laUniversidad Latinoamericana”- HISULA, sobre “Laindependencia americana: textos enseñanza eimaginarios escolares en Colombia y España. 2009 a2011”

Revista historia de la educación Latinoamericana No 16

Esta publicación se ha dedicado al tema monográficode los textos escolares. La relevancia del tema laasumimos por las investigaciones que sobre estecontenido viene desarrollando un equipo desde Franciay España. Esta red presenta vínculos con investigadores,en América Latina, donde podemos destacar enVenezuela al Dr. José Pascual Mora, presidente de laSociedad de Historia de la Educación Latinoamericana.Pero hay que decir que principalmente, se motivó eltema monográfico, por la investigación que realizó elGrupo de investigación: “Historia y Prospectiva de laUniversidad Latinoamericana”- HISULA, sobre “Laindependencia americana: textos enseñanza eimaginarios escolares en Colombia y España. 2009 a2011”

Revista historia de la educación Latinoamericana No 16

Universidad Pedagógica y Tecnológica de ColombiaSociedad de Historia de la Educación LatinoamericanaRUDECOLOMBI

Hidden Markov Models for Activity Detection in Atrial Fibrillation Electrograms

Proceeding of 2020 Computing in Cardiology (CinC 2020), 13-16 September 2020, Rimini, ItalyActivity detection in atrial fibrillation (AF) electrograms (EGMs) is a key concept to understand the mechanisms of this frequent arrhythmia and design new strategies for its treatment. We present a new method that employs Hidden Markov Models (HMMs) to identify activity presence in bipolar EGMs. The method is fully unsupervised and hence it does not require labeled training data. The HMM activity detection method was validated and compared to the non-linear energy operator (NLEO) method for a set of manually annotated EGMs. The HMM performed better than the NLEO and exhibited more robustness in the presence of low voltage fragmented EGMs.This study was supported by grants PI18/01895 from the Instituto de Salud Carlos III, and RD16/0011/0029 Red de Terapia Celular from the Instituto de Salud Carlos III, the projects RTI2018-099655-B-I00; TEC2017-92552-EXP; PID2019-108539RB-C22, Y2018/TCS-4705, and the support of NVIDIA Corporation with the donation of the Titan V GPU used during this research.Publicad

Structural remodeling and rotational activity in persistent/long-lasting atrial fibrillation: gender-effect differences and impact on post-ablation outcome

Background: Structural and post-ablation gender differences are reported in atrial fibrillation (AF). We analyzed the gender differences in structural remodeling and AF mechanisms in patients with persistent/long-lasting AF who underwent wide area circumferential pulmonary vein isolation (WACPVI). Materials and Methods: Ultra-high-density mapping was used to study atrial remodeling and AF drivers in 85 consecutive patients. Focal and rotational activity (RAc) were identified with the CartoFinder system and activation sequence analysis. The impact of RAc location on post-ablation outcomes was analyzed. Results: This study included 64 men and 21 women. RAc was detected in 73.4% of men and 38.1% of women (p = 0.003). RAc patients had higher left atrium (LA) voltage (0.64 ± 0.3 vs. 0.50 ± 0.2 mV; p = 0.01), RAc sites had higher voltage than non-RAc sites 0.77 ± 0.46 vs. 0.53 ± 0.37 mV (p < 0.001). Women had lower LA voltage than men (0.42 vs. 0.64 mV; p < 0.001), including pulmonary vein (PV) antra (0.16 vs. 0.30 mV; p < 0.001) and posterior wall (0.34 vs. 0.51 mV; p < 0.001). RAc in the posterior atrium was recorded in few women (23.8 vs. 54.7% in men; p = 0.014). AF recurrence rate was higher in patients with RAc outside WACPVI than those with all RAc inside WACPVI or no RAc (63.4 vs. 11.1 and 31.0%; p = 0.008 and p = 0.01). Comparison of selected patients using propensity score matching confirmed lower atrial voltage (0.4 ± 0.2 vs. 0.7 ± 0.3 mV; p = 0.007) and less RAc (38 vs. 75%; p = 0.02) in women. Conclusion: Women have shown more advanced structural remodeling at ablation, which is associated with a lower incidence of RAc (usually located outside the WACPVI). These findings could explain post-ablation gender differences.This study was supported by the Instituto de Salud Carlos III, Madrid, Spain (PI18/01895 and DTS21/00064), Red de Terapia Celular from the Instituto de Salud Carlos III, Madrid, Spain (RD16/0011/0029), Ricors "Red de Investigación Cooperativa Orientada a Resultados en Salud" RICORS TERAV (RD21/0017/0002), and the Sección del Ritmo de la Sociedad Española de Cardiología (Grant: Beca de la Asociacion del Ritmo para formación en investigacion post-residencia en centros españoles de la Sección del Ritmo de la Sociedad Española de Cardiología), Madrid, Spain

Independent COL5A1 Variant in Cats with Ehlers-Danlos Syndrome

We investigated four cats with similar clinical skin-related signs strongly suggestive of Ehlers-Danlos syndrome (EDS). Cases no. 1 and 4 were unrelated and the remaining two cases, no. 2 and 3, were reportedly siblings. Histopathological changes were characterized by severely altered dermal collagen fibers. Transmission electron microscopy in one case demonstrated abnormalities in the collagen fibril organization and structure. The genomes of the two unrelated affected cats and one of the affected siblings were sequenced and individually compared to 54 feline control genomes. We searched for private protein changing variants in known human EDS candidate genes and identified three independent heterozygous COL5A1 variants. COL5A1 is a well-characterized candidate gene for classical EDS. It encodes the proα1 chain of type V collagen, which is needed for correct collagen fibril formation and the integrity of the skin. The identified variants in COL5A1 are c.112_118+15del or r.spl?, c.3514A>T or p.(Lys1172*), and c.3066del or p.(Gly1023Valfs*50) for cases no. 1, 2&3, and 4, respectively. They presumably all lead to nonsense-mediated mRNA decay, which results in haploinsufficiency of COL5A1 and causes the alterations of the connective tissue. The whole genome sequencing approach used in this study enables a refinement of the diagnosis for the affected cats as classical EDS. It further illustrates the potential of such experiments as a precision medicine approach in animals with inherited diseases

A genome-scale in vivo loss-of-function screen identifies Phf6 as a lineage-specific regulator of leukemia cell growth

We performed a genome-scale shRNA screen for modulators of B-cell leukemia progression in vivo. Results from this work revealed dramatic distinctions between the relative effects of shRNAs on the growth of tumor cells in culture versus in their native microenvironment. Specifically, we identified many “context-specific” regulators of leukemia development. These included the gene encoding the zinc finger protein Phf6. While inactivating mutations in PHF6 are commonly observed in human myeloid and T-cell malignancies, we found that Phf6 suppression in B-cell malignancies impairs tumor progression. Thus, Phf6 is a “lineage-specific” cancer gene that plays opposing roles in developmentally distinct hematopoietic malignancies.Massachusetts Institute of Technology. Department of Biology (Training Grant)National Cancer Institute (U.S.). Integrative Cancer Biology Program (U54-CA112967-06)National Institutes of Health (U.S.) (RO1-CA128803-05

Requirements and Specifications for the Orchestration of Network Intelligence in 6G

Next-generation mobile networks are expected to flaunt highly (if not fully) automated management. Network Intelligence (NI) will be the key enabler for such a vision, empowering myriad of orchestrators and controllers across network domains. In this paper, we elaborate on the DAEMON architectural model, which proposes introducing a NI Orchestration layer for the effective end-to-end coordination of NI instances deployed across the whole mobile network infrastructure. Specifically, we first outline requirements and specifications for NI design that stem from data management, control timescales, and network technology characteristics. Then, we build on such analysis to derive initial principles for the design of the NI Orchestration layer, focusing on (i) proposals for the interaction loop between NI instances and the NI Orchestrator, and (ii) a unified representation of NI algorithms based on an extended MAPE-K model. Our work contributes to the definition of the interfaces and operation of a NI Orchestration layer that foster a native integration of NI in mobile network architectures.This project has received funding from the European Unions Horizon 2020 research and innovation programme under grant agreement no.101017109 DAEMON

Isolation of tenebrio molitor enterobacteriaceae (coleoptera: tenebrionidae) as degrading organisms of expanded polystyrene under laboratory conditions

El proyecto de investigación se realizó con la premisa de hacer uso de la biotecnología como una alternativa de solución para un problema aún difícil de resolver como es la biodegradación de plásticos de un solo uso. El objetivo fue aislar bacterias de la microbiota intestina de las larvas de Tenebrio molitor como organismos degradadores del poliestireno expandido, identificar el género de bacterias aisladas, determinar el volumen de poliestireno consumido por las larvas como fuente única de alimentación, bajo condiciones de laboratorio y finalmente valorar la composición bioquímica de los desechos generados como parte del proceso de digestión de las larvas de Tenebrio molitor. El tracto digestivo extraído fue introducido en un microtubo eppendorf con 0,2 mL de agua peptonada al 10% y usando el vórtex por 30s se logró separar los microorganismos de las paredes del tejido, se hizo un cultivo en Chromagar y el repique en agar telurito de K a 37ºC por 48 horas, para la identificación de la especie de las enterobacterias aisladas. De acuerdo con la coloración de las colonias aisladas, y por el patrón de identificación, se pudo determinar que se trataba de la especie Enterococcus faecalis ATCC 29212 (Gram+). La composición bioquímica de los desechos generados como parte del proceso de digestión de las larvas, por espectrometría, evidenció que los polímeros sintéticos habían sido digeridos por las enterobacterias generó excretas contenidas en O, N, P y K demostrando la capacidad de convertir sustancias no biodegradables, en sustancias químicas potencialmente asimilables.The research project was carried out with the premise of making use of biotechnology as an alternative solution for a problem that is still difficult to solve, such as the biodegradation of single-use plastics. The objective was to isolate bacteria from the intestinal microbiota of the larvae of Tenebrio molitor as degrading organisms of expanded polystyrene, identify the genus of isolated bacteria, determine the volume of polystyrene consumed by the larvae as the sole source of food, under conditions of laboratory and finally assess the biochemical composition of the waste generated as part of the digestion process of the larvae of Tenebrio molitor. The extracted digestive tract was introduced into an eppendorf microtube with 0.2 mL of 10% peptone water and using the vortex for 30s it was possible to separate the microorganisms from the tissue walls, a culture was made on Chromagar and the peal on tellurite agar of K at 37ºC for 48 hours, for the identification of the species of the isolated Enterobacteriaceae. According to the coloration of the isolated colonies, and by the identification pattern, it could be determined that it was the species Enterococcus faecalis ATCC 29212 (Gram +). The biochemical composition of the waste generated as part of the digestion process of the larvae, by spectrometry, showed that the synthetic polymers had been digested by the Enterobacteriaceae generated excreta contained in O, N, P and K demonstrating the ability to convert non-biodegradable substances, in potentially assimilable chemicals