Type III secretion: The bacteria-eukaryotic cell express

Type III secretion (T3S) is an export pathway used by Gram-negative pathogenic bacteria to inject bacterial proteins into the cytosol of eukaryotic host cells. This pathway is characterized by (i) a secretion nanomachine related to the bacterial flagellum, but usually topped by a stiff needle-like structure; (ii) the assembly in the eukaryotic cell membrane of a translocation pore formed by T3S substrates; (iii) a non-cleavable N-terminal secretion signal; (iv) T3S chaperones, assisting the secretion of some substrates; (v) a control mechanism ensuring protein delivery at the right place and time. Here, we review these different aspects focusing in open questions that promise exciting findings in the near futur

Effect of Protein Level, Lysine and Oats in Diets for Growing-Finishing Pigs

Previous research has shown that the protein content of swine diets can be reduced approximately two percent if the diet is supplemented with lysine and if the diet is not deficient in other amino acids. Corn-soybean meal diets that are reduced more than two percent in protein may become deficient in the amino acid tryptophan. Oats contains more lysine and tryptophan but less energy than corn. However, dietary levels of 20 to 30% oats have generally not affected pig performance. The objectives of this experiment were to evaluate the protein and lysine needs of growing-finishing pigs and to determine the value of oats as an amino acid source in low protein diets containing 20% oats as a replacement for corn

Who wants to conserve remaining forest fragments in the Manompana Corridor?

Contiguous forests in Madagascar are continuously converted into forest fragments due to deforestation, and dispersed into landscape mosaics dominated by agriculture. These fragments are of increasing importance for biodiversity conservation as well as for the well being of rural inhabitants, providing a high diversity of timber and non- timber forest products. An increasing number of international projects are therefore trying to preserve remaining forests and to transfer the management of these forests to local communities. However, it is not known how important the preservation of forest fragments is to local people. We therefore explore the importance of forest fragments as a source of cash income to different groups separated by wealth level and access to forest resources. A multi-method research approach was taken, based on score application exercises as well as interviews with individual households and focus groups. Our study site was located at the east coast of Madagascar in the Manompana corridor. Results show that some groups are significantly more interested in the preservation of forest fragments than others. Interest is significantly related to the wealth of local inhabitants as well as to the walking distance between villages and forest resources. Nevertheless, interest in resource preservation does not depend on how important fragments are to local people, but rather on the awareness about resource scarcity.  Résumé En raison d’une forte déforestation sur la côte est de Madagascar, de nombreux massifs forestiers d’un seul tenant et de vastes écosystèmes interconnectés ont été détruits, laissant des fragments de forêts qui s’intègrent dans une mosaïque paysagère dominée par l’agriculture. Ces fragments gagnent en importance. Ils jouent un rôle de premier plan dans les réseaux de biodiversité en assurant un certain niveau de connectivité. Mais les fragments sont essentiels au bien - être de la population locale, fournissant produits et services pour la consommation quotidienne ou donnant accès à un revenu monétaire. Sur un plan global, aussi bien les organisations de protection de la nature que les milieux scientifiques essayent d’endiguer la déforestation. Depuis les années 1996 la politique nationale à Madagascar a généré lois et processus visant à transférer la gestion des ressources forestières de l’Etat aux communautés locales. Cependant, il n’a pas été possible, jusqu’à ce jour, d’atténuer l’ampleur de la destruction et de la fragmentation des forêts pluviales de l’île. Plus encore, à l’heure actuelle la perception de l’importance des fragments de forêts n’est pas connue par la population. Un projet de recherche a été lancé pour contribuer à combler cette lacune, dans le corridor de Manompana, sur la côte. Les buts de ce projet étaient (i) d’explorer l’importance des fragments de forêts pour les revenus monétaires de la population locale et (ii) d’analyser la perception de l’importance des fragments de forêts par la population locale. Les recherches se sont déroulées dans quatre villages situés à des distances différentes du grand massif forestier. La population locale a été répartie en différentes catégories de niveau de vie et en fonction de la distance à parcourir entre les villages et la forêt. Cette approche a permis d’étudier le rôle de la forêt quant aux revenus monétaires des différents groupes de la population. Nous avons également cherché à établir un lien entre l’ampleur des revenus monétaires et un intérêt à conserver les fragments de forêts qui subsistent. Nos méthodes de recherche font recours à des exercices de « scoring », à des discussions avec des groupes ciblés et à des enquêtes de ménages. Il ressort des analyses que certains groupes ont un intérêt à conserver les fragments forestiers. Cet intérêt est significativement lié, d’une part, au niveau de vie de la population, d’autre part, à la distance entre le village et le massif forestier. Cependant, l’intérêt à conserver les fragments de forêts est plus fortement lié à la conscience de la finitude des ressources forestières qu’au montant des revenus monétaires que la population peut tirer des produits forestiers.

The Use of Financial Literacy for Growing Personal Finance

Financial literacy played an important role for everyone in managing personal finances.This research aimed to determine how the level of financial literacy in students S1 Facultyof Economics and Business, Universitas Pasundan and investigate what factors are influencingit. The observed respondents were students from the Faculty of Economics andBusiness, Universitas Pasundan. The research data was collected through questionnaires,descriptive analysis, and test multinomial logit. Based on the results of the researchshowed that the level of financial literacy from undergraduate students UniversitasPasundan was in the low category. Financial literacy was determined by gender, GreaterAcademic Achievement (GPA), parental education level, and parental income level;,whereas for age, year of study and residence do not contribute to the research model. Theresults of this study were expected to support personal financial planning of students inimproving the skills of reading, analyzing, and managing their own finances, thus avoidingthe daily financial problems

Energy-sensitive imaging detector applied to the dissociative recombination of D2H+

We report on an energy-sensitive imaging detector for studying the fragmentation of polyatomic molecules in the dissociative recombination of fast molecular ions with electrons. The system is based on a large area (10 cm x 10 cm) position-sensitive, double-sided Si-strip detector with 128 horizontal and 128 vertical strips, whose pulse height information is read out individually. The setup allows to uniquely identify fragment masses and is thus capable of measuring branching ratios between different fragmentation channels, kinetic energy releases, as well as breakup geometries, as a function of the relative ion-electron energy. The properties of the detection system, which has been installed at the TSR storage ring facility of the Max-Planck Institute for Nuclear Physics in Heidelberg, is illustrated by an investigation of the dissociative recombination of the deuterated triatomic hydrogen cation D2H+. A huge isotope effect is observed when comparing the relative branching ratio between the D2+H and the HD+D channel; the ratio 2B(D2+H)/B(HD+D), which is measured to be 1.27 +/- 0.05 at relative electron-ion energies around 0 eV, is found to increase to 3.7 +/- 0.5 at ~5 eV.Comment: 11 pages, 12 figures, submitted to Physical Review

CD69 is a TGF-β/1α,25-dihydroxyvitamin D3 target gene in monocytes

CD69 is a transmembrane lectin that can be expressed on most hematopoietic cells. In monocytes, it has been functionally linked to the 5-lipoxygenase pathway in which the leukotrienes, a class of highly potent inflammatory mediators, are produced. However, regarding CD69 gene expression and its regulatory mechanisms in monocytes, only scarce data are available. Here, we report that CD69 mRNA expression, analogous to that of 5-lipoxygenase, is induced by the physiologic stimuli transforming growth factor-β (TGF-β) and 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3) in monocytic cells. Comparison with T- and B-cell lines showed that the effect was specific for monocytes. CD69 expression levels were increased in a concentration-dependent manner, and kinetic analysis revealed a rapid onset of mRNA expression, indicating that CD69 is a primary TGF-β/1α,25(OH)2D3 target gene. PCR analysis of different regions of the CD69 mRNA revealed that de novo transcription was initiated and proximal and distal parts were induced concomitantly. In common with 5-lipoxygenase, no activation of 0.7 kb or ~2.3 kb promoter fragments by TGF-β and 1α,25(OH)2D3 could be observed in transient reporter assays for CD69. Analysis of mRNA stability using a transcription inhibitor and a 3′UTR reporter construct showed that TGF-β and 1α,25(OH)2D3 do not influence CD69 mRNA stability. Functional knockdown of Smad3 clearly demonstrated that upregulation of CD69 mRNA, in contrast to 5-LO, depends on Smad3. Comparative studies with different inhibitors for mitogen activated protein kinases (MAPKs) revealed that MAPK signalling is involved in CD69 gene regulation, whereas 5-lipoxygenase gene expression was only partly affected. Mechanistically, we found evidence that CD69 gene upregulation depends on TAK1-mediated p38 activation. In summary, our data indicate that CD69 gene expression, conforming with 5-lipoxygenase, is regulated monocyte-specifically by the physiologic stimuli TGF-β and 1α,25(OH)2D3 on mRNA level, although different mechanisms account for the upregulation of each gene