Effects of carbon source, carbon concentration and culture conditions on in vitro rooting of Pinus pinea L. microshoots

In stone pine (Pinus pinea L.), clonal propagation via adventitious shoot formation from cotyledons has been reported before but rooting of these shoots is poor. The number of rooted shoots had low frequency limiting the used of micropropagation protocols as a choice for mass propagation of superior genotypes. Therefore the main objective of the present work was to increase the number and quality of roots per shoot in order to ensure the survival and growth of a great number of plants in the acclimation phase. To achieve this, different combinations of carbon source (sucrose or glucose) at different concentrations, under different environmental conditions (temperature and light), were tested in the induction and expression phases of the adventitious root formation. Shoots of different clones obtained via organogenesis have been employed for the experiments. Observations were made on the rooting percentage, root length and number of roots per shoot. The results showed a general increased of the number of roots per shoot and an earlier root formation when glucose was used as a carbon source. However, there were no differences in the percentage of rooting between the carbons sources tested. The best results were obtained using 0.117 M of glucose and dark treatment combined with 19°C during the induction phase of the rhizogenic process. Light and low sugar concentration proved to be beneficial for the expression phase, increasing the root length. A remarkable interclonal difference in the ability to form roots was observed. It was possible to obtain a rooting percentage of more than 75% in several of the tested clones

Viral genetic clustering and transmission dynamics of the 2022 mpox outbreak in Portugal

Pathogen genome sequencing during epidemics enhances our ability to identify and understand suspected clusters and investigate their relationships. Here, we combine genomic and epidemiological data of the 2022 mpox outbreak to better understand early viral spread, diversification and transmission dynamics. By sequencing 52% of the confirmed cases in Portugal, we identified the mpox virus sublineages with the highest impact on case numbers and fitted them into a global context, finding evidence that several international sublineages probably emerged or spread early in Portugal. We estimated a 62% infection reporting rate and that 1.3% of the population of men who have sex with men in Portugal were infected. We infer the critical role played by sexual networks and superspreader gatherings, such as sauna attendance, in the dissemination of mpox virus. Overall, our findings highlight genomic epidemiology as a tool for the real-time monitoring and control of mpox epidemics, and can guide future vaccine policy in a highly susceptible population.info:eu-repo/semantics/publishedVersio

O proxecto INclúe: innovación para a Inclusión Social en Galicia, de Emaús Fundación Social

El Proyecto INclúe se desarrolla en Galicia (mayo 2014 - septiembre 2015). Se trata de una iniciativa gestada desde la colaboración interinstitucional, a partir de la constitución de un partenariado conformado por la Dirección Xeral de Inclusión Social de la Xunta de Galicia, Emaús Fundación social y la Consultora EOSA (Estrategia y Organización, s.a.). El proyecto contó también con diferentes entidades colaboradoras: 14 entidades sociales con experiencia en Inclusión Social, 4 corporaciones locales y el Consorcio Gallego de servicios de Igualdad y Bienestar. Emaús, como entidad promotora del proyecto, asumió el reto de identificar un nuevo marco conceptual de referencia, el Modelo INclúe, que redefina las claves de una atención de calidad para todas las entidades público-privadas que intervienen en el ámbito de la inclusión social. Estas claves fueron identificadas y definidas conjuntamente entre las entidades socias, las entidades colaboradoras y personas afectadas por situaciones de exclusión social (a través de reuniones de trabajo participativo). Entre los resultados de este trabajo pueden destacarse dos publicaciones: Modelo INclúe, donde se analiza la fundamentación teórica de las claves para una atención de calidad y El Manual INclúe, dirigido a profesionales de entidades públicas y privadas que trabajen por inclusión social. Paralelamente se ha diseñado una herramienta informática (http://valoracion.emausinclue.com/inclue.zip) que permite la valoración técnica y objetiva de diferentes grados de exclusión social, susceptible de ser aplicada por las entidades públicas y privadas que operan en el ámbito de la inclusión social en Galicia, y que puede ser replicable en otras comunidades autónomas

Receita funcional na Atenção Primária à Saúde: um relato de experiência

o sucesso do controle das doenças crônicas não transmissíveis, o qual pode estar relacionado à qualidade da prescrição, à forma de explicação para o paciente ou à compreensão deste para com o que foi prescrito. Dessa forma, os pictogramas (símbolos que ilustram e descrevem a informação), auxiliam na comunicação para a adesão do tratamento e controle das morbidades. O objetivo deste trabalho é relatar a experiência de controle da hipertensão arterial sistêmica (HAS) de um paciente, através da utilização de receitas funcionais. Foi realizado um relato de experiência de uma ação de intervenção realizada por acadêmicos de Medicina junto a uma residente de Medicina de Família e Comunidade, em uma unidade de Saúde da Família, em Maceió-AL. Durante consulta médica, ao avaliar um paciente portador de HAS, notou-se elevados níveis da pressão arterial (PA) registrados no prontuário e nas anotações diárias da pressão arterial dos últimos dois meses, apesar de estarem prescritas doses plenas de anti-hipertensivos. Foi constatado que o paciente fazia uso incorreto das medicações, por não saber ler. Diante da situação, os autores elaboraram uma receita funcional, individualizada, de acordo com o entendimento do paciente, por meio de pictogramas, os quais representavam as refeições e atividades relacionadas aos horários em que as medicações deveriam ser administradas, além da marcação das cartelas de medicamentos com cores iguais às registradas na receita. ​O método foi explicado ao paciente, o qual retornou     após um mês, com níveis normais de PA. Conclui-se que, através da implementação da receita funcional, foram obtidos resultados positivos em curto prazo no controle de uma doença crônica relevante, evidenciando que a compreensão da prescrição repercute na segurança e no sucesso do tratamento, tendo o potencial de reduzir complicações futuras. Além disso, para a residente e para os acadêmicos envolvidos, ao elaborarem e implantarem um método alternativo de prescrição e comunicação com o paciente, houve significativo ganho do ponto de vista do aprendizado

Educación Superior y Género: Una aproximación desde la UNR

Nos acercamos en esta oportunidad para compartir la tercera producción del Programa Género y Universidad de la Universidad Nacional de Rosario, denominada “Educación Superior y Género: Una aproximación desde la UNR”. Una gran red temática que apunta a la información, la reflexión y la apropiación, conformando un espacio nodal de interacción, para ser una propuesta de trabajo en cada espacio de acción.Universidad Nacional de Rosari

Detection of mutations in GATA1 gene using automated denaturing high-performance liquid chromatography and direct sequencing in children with Down syndrome

Denaturing high-performance liquid chromatography (dHPLC) was developed to screen DNA variations by separating heteroduplex and homoduplex DNA fragments by ion-pair reverse-phase liquid chromatography. in this study, we have evaluated the dHPLC screening method and direct sequencing for the detection of GATA1 mutations in peripheral blood and bone marrow aspirates samples from children with Down syndrome (DS). Cases were ascertained consecutively as part of an epidemiological study of DS and hematological disorders in Brazil. A total of 130 samples corresponding to 115 children with DS were analysed using dHPLC and direct sequencing methods to detect mutations in GATA1 exons 2, 3 and 4 gene sequences. the overall detection rate of sequencing and dHPLC screening methods was similar. Twenty mutations were detected in exon 2 and one mutation in exon 3 (c.231_232 dupGT) sequences of acute megakaryoblastic leukemia and transient leukemia samples. Four GATA1 mutations were newly described [c.155CG; c.156_178 del23 bp; c.29_30 del GG; c.182CA and c.151AT,c.153_162 del 10 bp). Out of four, three had single nucleotide change. in conclusion, our results indicate that dHPLC is an efficient and valuable tool for GATA1 mutational analysis.Inst Nacl Canc, Div Genet CPq, BR-20331050 Rio de Janeiro, BrazilHosp Darcy Vargas, Pediat Oncohematol Serv, São Paulo, BrazilSoc Oncol Bahia, Salvador, BrazilCtr Infantil Invest Hematol D Boldrini, São Paulo, BrazilHosp Santa Casa Misericordia, Pediat Oncohematol Serv, Itabuna, Bahia, BrazilHosp Martagao Gesteira, Pediat Oncohematol Serv, Salvador, BA, BrazilUniv Santa Maria Rio Grande Sul, Dept Hematol, Porto Alegre, RS, BrazilHosp Joana Gusmao Florianopolis, Pediat Oncohematol Serv, Santa Catarina, BrazilUniversidade Federal de São Paulo, Inst Oncol Pediat, São Paulo, BrazilHosp AC Camargo Fund Antonio Prudente, Pediat Oncohematol Serv, São Paulo, BrazilHosp Canc Cascavel, UOPECCAN, Cascavel, Parana, BrazilHosp Napoleao Laureano, Joao Pessoa, Paraiba, BrazilUFRJ, Inst Pediat & Puericultura Martagao Gesteira, Rio de Janeiro, Paraiba, BrazilHosp Serv Estado Rio de Janeiro, Dept Pediat, Rio de Janeiro, BrazilUniversidade Federal de São Paulo, Inst Oncol Pediat, São Paulo, BrazilWeb of Scienc

Viral genetic clustering and transmission dynamics of the 2022 mpox outbreak in Portugal.

Acknowledgements: We gratefully acknowledge the engagement and willingness of all participants to share information critical to the investigation. We are grateful to the authors and laboratories that originated and submitted the genetic sequences released in GenBank. The acquisition of equipment associated with whole-genome sequencing used in this study (including the Illumina NextSeq 2000) was funded by the HERA (Human and Environmental Risk Assessment) project (Grant/2021/PHF/23776), supported by the European Commission through the European Centre for Disease Prevention and Control (ECDC), and partially funded by the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020 (Operational Programme for Competitiveness and Internationalisation (POCI)), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020) under the PORTUGAL 2020 Partnership Agreement through the European Regional Development Fund (ERDF), and by the Portuguese Science and Technology Foundation (FCT). This study was also supported by the ERINHA-Advance project (funded by the European Union’s Horizon 2020 Research & Innovation program, grant agreement no. 824061) and benefited from co-funding from the European Union’s Horizon 2020 Research and Innovation programme under grant agreement no. 773830 (One Health European Joint Programme), in particular by the co-funding of the post-doctoral fellowships of J.S.D. and V.M. and the development of INSaFLU. We also thank M. Pinheiro (iBiMED at the Universidade de Aveiro) for his continuous support in updating the INSaFLU platform and the Infraestrutura Nacional de Computação Distribuída (INCD) for providing computational resources for testing it. INCD was funded by the FCT and FEDER under the project 22153-01/SAICT/2016. M.P.D. is funded by the Gates Cambridge Scholarship (no. OPP1144). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. The GAT-Intendente team also thanks A. Vasques, L. Fortuna, J. Moreira, I. Correia and Á. Baginha.Pathogen genome sequencing during epidemics enhances our ability to identify and understand suspected clusters and investigate their relationships. Here, we combine genomic and epidemiological data of the 2022 mpox outbreak to better understand early viral spread, diversification and transmission dynamics. By sequencing 52% of the confirmed cases in Portugal, we identified the mpox virus sublineages with the highest impact on case numbers and fitted them into a global context, finding evidence that several international sublineages probably emerged or spread early in Portugal. We estimated a 62% infection reporting rate and that 1.3% of the population of men who have sex with men in Portugal were infected. We infer the critical role played by sexual networks and superspreader gatherings, such as sauna attendance, in the dissemination of mpox virus. Overall, our findings highlight genomic epidemiology as a tool for the real-time monitoring and control of mpox epidemics, and can guide future vaccine policy in a highly susceptible population