Very Low Nitrogen Leaching in Grazed Ley-Arable-Systems in Northwest Europe

High input dairy farms that are located on sandy soils in northwest Europe are predisposed to substantial nitrate leaching during a surplus of winter precipitation. Leys within integrated crop-livestock systems play an important role in soil fertility, soil C sequestration and soil N mineralization potentials. Therefore, leys are a feasible option that can be utilized to reduce local N losses to the environment, especially following maize grown for silage. We hypothesize that grass-clover leys ensure low nitrate leaching losses even when grazed intensively. The extent to which NO3-leaching occurred across seven different pasture management systems in terms of their sward composition, cutting, grazing, fertilization and combinations thereof was investigated in integrated animal-crop grazing systems over three winter periods (2017/2018, 2018/2019 and 2019/2020). The observed grazed systems were comprised of cut-used- and grazed grass-clover swards (0, 1 and 2 years after establishment following cereals), a catch crop grazed late in the year as well as a cut-used permanent grassland for comparison. Overall, all treatments resulted in nitrate leaching losses that did not exceed the WHO-threshold (25 mg nitrate/L). The highest level of NO3-leaching was observed in the catch crop system and the lowest in cut-used permanent grassland, with NO3-N losses of 19.6 ± 5.3 and 2.1 ± 0.3 kg NO3-N ha−1 year−1. Annual herbage yields were in the range of 0.9 to 12.4 t DM ha−1 and nitrogen yields varied between 181 ± 51 and 228 ± 66 kg N ha−1 during the study period. The highest herbage-N-yields were observed from the 1- and 2-year-old grass-clover leys. The highest N-field-balance was observed for the grazed leys and the lowest for the cut-used permanent grassland. However, no correlation was found between the highly positive field-N-balance and the amount of NO3-leached

Environmental Impact of Rotationally Grazed Pastures at Different Management Intensities in South Africa

Nitrogen fertilization, irrigation and concentrate feeding are important factors in rotational pasture management for dairy farms in South Africa. The extent to which these factors affect environmental efficiency is subject to current and intense debate among scientists. A three-year field study was conducted to investigate the yield response of different N-fertilizer treatments (0 (N0), 220 (N20), 440 (N40), 660 (N60) and 880 (N80) kg N ha-1 year-1) on grazed pastures and to calculate the carbon footprint (CF) of milk produced. Excessive N-fertilization (N60 and N80) did not increase herbage dry matter and energy yields from pastures. However, N80 indicated the highest N-yield but at the same time also the highest N surpluses at field level. A maximum fertilizer rate of 220 kg ha-1 year-1 (in addition to excreted N from grazing animals) appears sufficient to ensure adequate herbage yields (~20 t DM ha-1 year-1) with a slightly positive field-N-balance. This amount will prevent the depletion of soil C and N, with low N losses to the environment, where adequate milk yields of ~17 t ECM ha-1 with a low CF (~1.3 kg CO2 kg ECM-1) are reached. Methane from enteric fermentation (~49% ± 3.3) and N2O (~16% ± 3.2) emissions from irrigated pastures were the main contributors to the CF. A further CF reduction can be achieved by improved N-fertilization planning, low emission irrigation techniques and strategies to limit N2O emissions from pasture soils in South Africa

Grazing under irrigation affects N2O-emissions substantially in South Africa

CITATION: Smit, H. P. J. et al. 2020. Grazing under irrigation affects N2O-emissions substantially in South Africa. Atmosphere, 11(9):925, doi:10.3390/atmos11090925.The original publication is available at https://www.mdpi.comFertilized agricultural soils serve as a primary source of anthropogenic N2O emissions. In South Africa, there is a paucity of data on N2O emissions from fertilized, irrigated dairy-pasture systems and emission factors (EF) associated with the amount of N applied. A first study aiming to quantify direct N2O emissions and associated EFs of intensive pasture-based dairy systems in sub-Sahara Africa was conducted in South Africa. Field trials were conducted to evaluate fertilizer rates (0, 220, 440, 660, and 880 kg N ha−1 year−1) on N2O emissions from irrigated kikuyu–perennial ryegrass (Pennisetum clandestinum–Lolium perenne) pastures. The static chamber method was used to collect weekly N2O samples for one year. The highest daily N2O fluxes occurred in spring (0.99 kg ha−1 day−1) and summer (1.52 kg ha−1 day−1). Accumulated N2O emissions ranged between 2.45 and 15.5 kg N2O-N ha−1 year−1 and EFs for mineral fertilizers applied had an average of 0.9%. Nitrogen in yielded herbage varied between 582 and 900 kg N ha−1. There was no positive effect on growth of pasture herbage from adding N at high rates. The relationship between N balance and annual N2O emissions was exponential, which indicated that excessive fertilization of N will add directly to N2O emissions from the pastures. Results from this study could update South Africa’s greenhouse gas inventory more accurately to facilitate Tier 3 estimates.https://www.mdpi.com/2073-4433/11/9/925Publisher's versio

Expression of ABC Efflux Transporters in Placenta from Women with Insulin-Managed Diabetes

Drug efflux transporters in the placenta can significantly influence the materno-fetal transfer of a diverse array of drugs and other xenobiotics. To determine if clinically important drug efflux transporter expression is altered in pregnancies complicated by gestational diabetes mellitus (GDM-I) or type 1 diabetes mellitus (T1DM-I), we compared the expression of multidrug resistance protein 1 (MDR1), multidrug resistance-associated protein 2 (MRP2) and the breast cancer resistance protein (BCRP) via western blotting and quantitative real-time polymerase chain reaction in samples obtained from insulin-managed diabetic pregnancies to healthy term-matched controls. At the level of mRNA, we found significantly increased expression of MDR1 in the GDM-I group compared to both the T1DM-I (p<0.01) and control groups (p<0.05). Significant changes in the placental protein expression of MDR1, MRP2, and BCRP were not detected (p>0.05). Interestingly, there was a significant, positive correlation observed between plasma hemoglobin A1c levels (a retrospective marker of glycemic control) and both BCRP protein expression (r = 0.45, p<0.05) and BCRP mRNA expression (r = 0.58, p<0.01) in the insulin-managed DM groups. Collectively, the data suggest that the expression of placental efflux transporters is not altered in pregnancies complicated by diabetes when hyperglycemia is managed; however, given the relationship between BCRP expression and plasma hemoglobin A1c levels it is plausible that their expression could change in poorly managed diabetes

Molecular Basis of Ligand Dissociation in β-Adrenergic Receptors

The important and diverse biological functions of β-adrenergic receptors (βARs) have promoted the search for compounds to stimulate or inhibit their activity. In this regard, unraveling the molecular basis of ligand binding/unbinding events is essential to understand the pharmacological properties of these G protein-coupled receptors. In this study, we use the steered molecular dynamics simulation method to describe, in atomic detail, the unbinding process of two inverse agonists, which have been recently co-crystallized with β1 and β2ARs subtypes, along four different channels. Our results indicate that this type of compounds likely accesses the orthosteric binding site of βARs from the extracellular water environment. Importantly, reconstruction of forces and energies from the simulations of the dissociation process suggests, for the first time, the presence of secondary binding sites located in the extracellular loops 2 and 3 and transmembrane helix 7, where ligands are transiently retained by electrostatic and Van der Waals interactions. Comparison of the residues that form these new transient allosteric binding sites in both βARs subtypes reveals the importance of non-conserved electrostatic interactions as well as conserved aromatic contacts in the early steps of the binding process

Simulated-Physiological Loading Conditions Preserve Biological and Mechanical Properties of Caprine Lumbar Intervertebral Discs in Ex Vivo Culture

Low-back pain (LBP) is a common medical complaint and associated with high societal costs. Degeneration of the intervertebral disc (IVD) is assumed to be an important causal factor of LBP. IVDs are continuously mechanically loaded and both positive and negative effects have been attributed to different loading conditions

Dealloying of Cobalt from CuCo Nanoparticles under Syngas Exposure

International audienceThe structure and composition of core−shell CuCo nanoparticles were found to change as a result of cleaning pretreatments and when exposed to syngas (CO + H 2) at atmospheric pressure. In situ X-ray absorption and photoelectron spectroscopies revealed the oxidation state of the particles as well as the presence of adsorbates under syngas. Transmission electron microscopy was used for ex situ analysis of the shape, elemental composition, and structure after reaction. The original core−shell structure was found to change to a hollow CuCo alloy after pretreatment by oxidation in pure O 2 and reduction in pure H 2. After 30 min of exposure to syngas, a significant fraction (5%) of the particles was strongly depleted in cobalt giving copper-rich nanoparticles. This fraction increased with duration of syngas exposure, a phenomenon that did not occur under pure CO or pure H 2. This study suggests that Co and Cu can each individually contribute to syngas conversion with CuCo catalysts

A global experiment on motivating social distancing during the COVID-19 pandemic

Finding communication strategies that effectively motivate social distancing continues to be a global public health priority during the COVID-19 pandemic. This cross-country, preregistered experiment (n = 25,718 from 89 countries) tested hypotheses concerning generalizable positive and negative outcomes of social distancing messages that promoted personal agency and reflective choices (i.e., an autonomy-supportive message) or were restrictive and shaming (i.e., a controlling message) compared with no message at all. Results partially supported experimental hypotheses in that the controlling message increased controlled motivation (a poorly internalized form of motivation relying on shame, guilt, and fear of social consequences) relative to no message. On the other hand, the autonomy-supportive message lowered feelings of defiance compared with the controlling message, but the controlling message did not differ from receiving no message at all. Unexpectedly, messages did not influence autonomous motivation (a highly internalized form of motivation relying on one’s core values) or behavioral intentions. Results supported hypothesized associations between people’s existing autonomous and controlled motivations and self-reported behavioral intentions to engage in social distancing. Controlled motivation was associated with more defiance and less long-term behavioral intention to engage in social distancing, whereas autonomous motivation was associated with less defiance and more short- and long-term intentions to social distance. Overall, this work highlights the potential harm of using shaming and pressuring language in public health communication, with implications for the current and future global health challenges

Cohort Profile: Burden of Obstructive Lung Disease (BOLD) study

The Burden of Obstructive Lung Disease (BOLD) study was established to assess the prevalence of chronic airflow obstruction, a key characteristic of chronic obstructive pulmonary disease, and its risk factors in adults (≥40 years) from general populations across the world. The baseline study was conducted between 2003 and 2016, in 41 sites across Africa, Asia, Europe, North America, the Caribbean and Oceania, and collected high-quality pre- and post-bronchodilator spirometry from 28 828 participants. The follow-up study was conducted between 2019 and 2021, in 18 sites across Africa, Asia, Europe and the Caribbean. At baseline, there were in these sites 12 502 participants with high-quality spirometry. A total of 6452 were followed up, with 5936 completing the study core questionnaire. Of these, 4044 also provided high-quality pre- and post-bronchodilator spirometry. On both occasions, the core questionnaire covered information on respiratory symptoms, doctor diagnoses, health care use, medication use and ealth status, as well as potential risk factors. Information on occupation, environmental exposures and diet was also collected

Very Low Nitrogen Leaching in Grazed Ley-Arable-Systems in Northwest Europe

High input dairy farms that are located on sandy soils in northwest Europe are predisposed to substantial nitrate leaching during a surplus of winter precipitation. Leys within integrated crop-livestock systems play an important role in soil fertility, soil C sequestration and soil N mineralization potentials. Therefore, leys are a feasible option that can be utilized to reduce local N losses to the environment, especially following maize grown for silage. We hypothesize that grass-clover leys ensure low nitrate leaching losses even when grazed intensively. The extent to which NO3-leaching occurred across seven different pasture management systems in terms of their sward composition, cutting, grazing, fertilization and combinations thereof was investigated in integrated animal-crop grazing systems over three winter periods (2017/2018, 2018/2019 and 2019/2020). The observed grazed systems were comprised of cut-used- and grazed grass-clover swards (0, 1 and 2 years after establishment following cereals), a catch crop grazed late in the year as well as a cut-used permanent grassland for comparison. Overall, all treatments resulted in nitrate leaching losses that did not exceed the WHO-threshold (25 mg nitrate/L). The highest level of NO3-leaching was observed in the catch crop system and the lowest in cut-used permanent grassland, with NO3-N losses of 19.6 &plusmn; 5.3 and 2.1 &plusmn; 0.3 kg NO3-N ha&minus;1 year&minus;1. Annual herbage yields were in the range of 0.9 to 12.4 t DM ha&minus;1 and nitrogen yields varied between 181 &plusmn; 51 and 228 &plusmn; 66 kg N ha&minus;1 during the study period. The highest herbage-N-yields were observed from the 1- and 2-year-old grass-clover leys. The highest N-field-balance was observed for the grazed leys and the lowest for the cut-used permanent grassland. However, no correlation was found between the highly positive field-N-balance and the amount of NO3-leached. This indicates a high N carry-over from grass-clover swards to the subsequent cash crop unit instead of increasing the risk of groundwater contamination from grazed leys in integrated animal crop-systems and underlines the eco-efficiency of dairy farming based on grazed ley systems