The transcriptome of metamorphosing flatfish

Background Flatfish metamorphosis denotes the extraordinary transformation of a symmetric pelagic larva into an asymmetric benthic juvenile. Metamorphosis in vertebrates is driven by thyroid hormones (THs), but how they orchestrate the cellular, morphological and functional modifications associated with maturation to juvenile/adult states in flatfish is an enigma. Since THs act via thyroid receptors that are ligand activated transcription factors, we hypothesized that the maturation of tissues during metamorphosis should be preceded by significant modifications in the transcriptome. Targeting the unique metamorphosis of flatfish and taking advantage of the large size of Atlantic halibut (Hippoglossus hippoglossus) larvae, we determined the molecular basis of TH action using RNA sequencing. Results De novo assembly of sequences for larval head, skin and gastrointestinal tract (GI-tract) yielded 90,676, 65,530 and 38,426 contigs, respectively. More than 57 % of the assembled sequences were successfully annotated using a multi-step Blast approach. A unique set of biological processes and candidate genes were identified specifically associated with changes in morphology and function of the head, skin and GI-tract. Transcriptome dynamics during metamorphosis were mapped with SOLiD sequencing of whole larvae and revealed greater than 8,000 differentially expressed (DE) genes significantly (p < 0.05) up- or down-regulated in comparison with the juvenile stage. Candidate transcripts quantified by SOLiD and qPCR analysis were significantly (r = 0.843; p < 0.05) correlated. The majority (98 %) of DE genes during metamorphosis were not TH-responsive. TH-responsive transcripts clustered into 6 groups based on their expression pattern during metamorphosis and the majority of the 145 DE TH-responsive genes were down-regulated. Conclusions A transcriptome resource has been generated for metamorphosing Atlantic halibut and over 8,000 DE transcripts per stage were identified. Unique sets of biological processes and candidate genes were associated with changes in the head, skin and GI-tract during metamorphosis. A small proportion of DE transcripts were TH-responsive, suggesting that they trigger gene networks, signalling cascades and transcription factors, leading to the overt changes in tissue occurring during metamorphosis

Transcriptome of the Atlantic halibut (Hippoglossus hippoglossus)

Although the Atlantic halibut (Hippoglossus hippoglossus) is an important commercial species, there is still a deficit with regard to the number of transcripts in the databases, which can be accessed and exploited for targeted candidate gene and pathway studies. In this study, the RNAs from head, skin and GI tract from different developmental stages were sequenced to generate 22,272 contigs of 500 base pairs or greater as a molecular resource for this specie

低酸素呼吸抑制に対する抑制神経伝達物質の関与

研究科: 千葉大学大学院医学研究科千大院医博甲第1061号修了年: 2000年博士(医学)千葉大

Physicochemical characterization and structural evaluation of a specific 2:1 cocrystal of naproxen-nicotinamide

研究科: 千葉大学大学院医学薬学府学位:千大院医薬博甲第薬148号博士(薬学)千葉大

第1301回千葉医学会例会・臓器制御外科学教室談話会

In house GI-tract-specific database enriched with genes involved in GI-tract development, morphogenesis and acid secretion. Protein name, Symbol, Accession number (no.), Organism and Biological role (when associated) are shown. (XLSX 24 kb

Birtingarmyndir kynjanna : er kynjaslagsíða í dagblöðum?

Viðfangsefni þessarar rannsóknar var að varpa ljósi á birtingu kynjanna í kosningaumfjöllunum fyrir Alþingiskosningarnar árið 2007. Einnig var rannsakað hvernig umræðan um jafnrétti kynjanna birtist í þessari umfjöllun, sem og hvort stjórnmálaflokkar fjalli með meðvituðum hætti um jafnréttismál. Við rannsóknina var stuðst við prentmiðla, dagblöðin Morgunblaðið, Fréttablaðið og Blaðið. Þýðið var 474 greinar úr þessum þremur blöðum sem allar tengdust kosningum. Gagnasöfnun fór þannig fram að greindar voru allar greinar í blöðunum, vikurnar 22. apríl til 28. apríl og svo síðasta vikan fyrir kosningar, 6. maí til 12. maí. Stuðst var við sérstakt skemablað sem útbúið var fyrir greininguna. Niðurstöður voru svo bornar saman við sambærilega rannsókn sem Þorgerður H. Þorvaldsdóttir gerði árið 2003, sem og rannsókn Birgis Guðmundssonar sem gerð var á viðhorfum starfandi blaðamanna í aðdraganda kosninganna árið 2007. Niðurstöður okkar leiddu í ljós að konur eru í minnihluta viðmælenda á öllum blöðunum, hvort sem um er að ræða viðmælendur eða frambjóðendur. Einnig kom fram að konur töluðu frekar um jafnrétti en karlar en lítið var rætt um jafnrétti almennt. Niðurstaðan leiddi líka í ljós að stjórnmálaflokkar fá mismikla umfjöllun í blöðunum þremur og má því gefa sér að enn megi finna áhrif flokksblaðamennsku. Þegar stjórnmálaflokkar voru skoðaðir í tengslum við jafnréttismál kom í ljós að Samfylkingin og Vinstri græn fjalla mest um jafnréttismál

Additional file 2: of The transcriptome of metamorphosing flatfish

Annotation of the skin transcriptome. Contig ID, transcript name, number of GOs and their description and enzymatic codes are shown for each contig. (XLSX 3090 kb

Additional file 8: of The transcriptome of metamorphosing flatfish

Significantly overrepresented Biological Process GO terms identified for the head transcriptome (FDR < 0.05). (DOC 116 kb

Additional file 4: of The transcriptome of metamorphosing flatfish

Annotation of the head transcriptome. Contig ID, transcript name, number of GOs and their description and enzymatic codes are shown for each contig. (XLSX 4280 mb