Annotation of the skin transcriptome. Contig ID, transcript name, number of GOs and their description and enzymatic codes are shown for each contig. (XLSX 3090 kb

Ana Gomes (1479244)

Deborah Power (3406547)

H. Smáradóttir (3406550)

Ivar Rønnestad (2622340)

Kurt Stueber (3406541)

M. Clark (2309017)

M. Thorne (3406544)

Mbaye Tine (335358)

Ricardo Alves (3266523)

Richard Reinhard (3406553)

The list of the top most significantly up-regulated genes between premetamorphic stage 5 and the Juvenile stage determined using a linear model in Bayseq with a Benjamin-Hochberg adjustment for multiple testing analysis with a cut-off set at 0.05 (FDR < 0.05). Contig name, Gene name, Accession number (no.), Organism and E-value are shown for each gene. (DOCX 75 kb

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Additional file 10: of The transcriptome of metamorphosing flatfish

Reactome pathway analysis for the 145 differential expressed TH-responsive transcripts identified when Atlantic halibut metamorphic stages were compared with the juvenile. Reactome analysis was performed using INTREPROSCAN accession numbers obtained from the functional annotation of the Atlantic halibut transcriptome with Blast2GO. (DOCX 18 kb

Additional file 13: of The transcriptome of metamorphosing flatfish

Quantitative RT-PCR (qPCR) of the relative expression of ribosomal protein L7 (RPL7), 40S ribosomal protein S30 (FAU); alpha-globin 1 (Gloα1), carboxypeptidase A2 (Cpa2), apolipoprotein A-I (ApoAI) and type I keratin isoform 2 (Krt1i2). Analysis of the indicated transcripts was performed in whole Atlantic halibut larvae during development (stage 5 to juvenile; n = 5). The results are presented as mean ± SEM of the normalized expression, using the geometric mean of the reference genes RPS4 and EFIAI. Different letters represent significantly different mean values (p < 0.05; one-way ANOVA). (PDF 188 kb

Additional file 15: of The transcriptome of metamorphosing flatfish

Specific primers used for qPCR gene expression analysis. Gene symbol, name and function are shown. The annealing temperature (Ta °C), amplicon length (bp), R2 and qPCR efficiency (%) are indicated for each primer pair. (DOCX 17 kb

Additional file 20: of The transcriptome of metamorphosing flatfish

In house GI-tract-specific database enriched with genes involved in GI-tract development, morphogenesis and acid secretion. Protein name, Symbol, Accession number (no.), Organism and Biological role (when associated) are shown. (XLSX 24 kb

Additional file 17: of The transcriptome of metamorphosing flatfish