Using Geographic Information System (GIs) Software to Predict Blackbird Roosting Locations in North Dakota

Cattail stands provide roosting and staging areas for large congregations of blackbirds in North Dakota in late summer and early fall. Since 1991, the U.S. Depamnent of Agriculture, Ammal and Plant Health Inspection Service, Wildlife Services (WS) program has conducted a cattail management program in North Dakota to alleviate blackbird damage to ripening sunflower. To extend the capabilities of the program, a geographical mformation system (GIS) will be incorporated to help WS personnel find blackbird roosts more effectively. We will use the GIs to construct field maps showing the association between areas of moderate to hgh sunilower damage (\u3e5%) and cattail-dominated wetland basins \u3e2 ha. Buffer distances comparable to the distances blackbirds typically travel to forage will be placed around sunflower planting areas susceptible to high damage. This will lrclp WS ynsu~ult.l Cutiw heir eKurh un locating cattail-dominated wetlands that should be enrolled in the management program and improving current and fuhlre blackbird damage management programs

Multi-ancestry GWAS reveals excitotoxicity associated with outcome after ischaemic stroke

During the first hours after stroke onset, neurological deficits can be highly unstable: some patients rapidly improve, while others deteriorate. This early neurological instability has a major impact on long-term outcome. Here, we aimed to determine the genetic architecture of early neurological instability measured by the difference between the National Institutes of Health Stroke Scale (NIHSS) within 6 h of stroke onset and NIHSS at 24 h. A total of 5876 individuals from seven countries (Spain, Finland, Poland, USA, Costa Rica, Mexico and Korea) were studied using a multi-ancestry meta-analyses. We found that 8.7% of NIHSS at 24 h of variance was explained by common genetic variations, and also that early neurological instability has a different genetic architecture from that of stroke risk. Eight loci (1p21.1, 1q42.2, 2p25.1, 2q31.2, 2q33.3, 5q33.2, 7p21.2 and 13q31.1) were genome-wide significant and explained 1.8% of the variability suggesting that additional variants influence early change in neurological deficits. We used functional genomics and bioinformatic annotation to identify the genes driving the association from each locus. Expression quantitative trait loci mapping and summary data-based Mendelian randomization indicate that ADAM23 (log Bayes factor = 5.41) was driving the association for 2q33.3. Gene-based analyses suggested that GRIA1 (log Bayes factor = 5.19), which is predominantly expressed in the brain, is the gene driving the association for the 5q33.2 locus. These analyses also nominated GNPAT (log Bayes factor = 7.64) ABCB5 (log Bayes factor = 5.97) for the 1p21.1 and 7p21.1 loci. Human brain single-nuclei RNA-sequencing indicates that the gene expression of ADAM23 and GRIA1 is enriched in neurons. ADAM23, a presynaptic protein and GRIA1, a protein subunit of the AMPA receptor, are part of a synaptic protein complex that modulates neuronal excitability. These data provide the first genetic evidence in humans that excitotoxicity may contribute to early neurological instability after acute ischaemic stroke. Ibanez et al. perform a multi-ancestry meta-analysis to investigate the genetic architecture of early stroke outcomes. Two of the eight genome-wide significant loci identified-ADAM23 and GRIA1-are involved in synaptic excitability, suggesting that excitotoxicity contributes to neurological instability after ischaemic stroke.Peer reviewe

Stroke genetics informs drug discovery and risk prediction across ancestries

Previous genome-wide association studies (GWASs) of stroke — the second leading cause of death worldwide — were conducted predominantly in populations of European ancestry1,2. Here, in cross-ancestry GWAS meta-analyses of 110,182 patients who have had a stroke (five ancestries, 33% non-European) and 1,503,898 control individuals, we identify association signals for stroke and its subtypes at 89 (61 new) independent loci: 60 in primary inverse-variance-weighted analyses and 29 in secondary meta-regression and multitrait analyses. On the basis of internal cross-ancestry validation and an independent follow-up in 89,084 additional cases of stroke (30% non-European) and 1,013,843 control individuals, 87% of the primary stroke risk loci and 60% of the secondary stroke risk loci were replicated (P < 0.05). Effect sizes were highly correlated across ancestries. Cross-ancestry fine-mapping, in silico mutagenesis analysis3, and transcriptome-wide and proteome-wide association analyses revealed putative causal genes (such as SH3PXD2A and FURIN) and variants (such as at GRK5 and NOS3). Using a three-pronged approach4, we provide genetic evidence for putative drug effects, highlighting F11, KLKB1, PROC, GP1BA, LAMC2 and VCAM1 as possible targets, with drugs already under investigation for stroke for F11 and PROC. A polygenic score integrating cross-ancestry and ancestry-specific stroke GWASs with vascular-risk factor GWASs (integrative polygenic scores) strongly predicted ischaemic stroke in populations of European, East Asian and African ancestry5. Stroke genetic risk scores were predictive of ischaemic stroke independent of clinical risk factors in 52,600 clinical-trial participants with cardiometabolic disease. Our results provide insights to inform biology, reveal potential drug targets and derive genetic risk prediction tools across ancestries

Evaluation of Registered Sunflower Insecticides as Candidate Blackbird Repellents

Blackbird damage to ripening sunflower is forcing some growers to plant alternative, albeit less profitable, crops. Currently, only Bird Shield® (a.i., methyl anthranilate) is registered as a bird repellent on ripening sunflower. However, field experiments with Bird Shield showed no consistent differences in damage levels between treated and untreated plots. During September and October 2003, we screened five insecticide formulations for feeding repellency using individually caged red-winged blackbirds. The tested insecticides are registered and currently available for use on ripening sunflower. They were Asana® XL, Baythroid® 2, Lorsban®-4E, Scout X-Tra®, and Warrior® T

Bird Damage to Corn and Sunflower in North Dakota

The last comprehensive field surveys of bird damage to sunflower in North Dakota were conducted in 1979 and 1980. Average economic damage across years was slightly over US $5.0 million. Sunflower prices have appreciably increased since these monetary losses were calculated. As corn acreages in North Dakota have increased, so have complaints from growers about blackbird damage to corn. Quantitative surveys of blackbird damage to corn, however, have never been conducted in North Dakota. The goal of our multi-year study is to quantify blackbird damage to sunflower and corn crops in the Prairie Pothole Region, the principal corn and sunflower growing area in North Dakota (Fig. 1)

Comparison of Lorsban®-4E and Caffeine Aerially Sprayed on Sunflower Plots for Bird Repellency

Ripening sunflower fields in the northern Great Plains provide blackbirds with easily accessible sources of high-energy food. As many growers can attest, blackbirds can be nearly impossible to discourage from foraging in favored fields. Repellents sometimes can be effective feeding deterrents, especially if alternative foraging sites are readily available

Using Geographic Information System (GIs) Software to Predict Blackbird Roosting Locations in North Dakota

Cattail stands provide roosting and staging areas for large congregations of blackbirds in North Dakota in late summer and early fall. Since 1991, the U.S. Depamnent of Agriculture, Ammal and Plant Health Inspection Service, Wildlife Services (WS) program has conducted a cattail management program in North Dakota to alleviate blackbird damage to ripening sunflower. To extend the capabilities of the program, a geographical mformation system (GIS) will be incorporated to help WS personnel find blackbird roosts more effectively. We will use the GIs to construct field maps showing the association between areas of moderate to hgh sunilower damage (\u3e5%) and cattail-dominated wetland basins \u3e2 ha. Buffer distances comparable to the distances blackbirds typically travel to forage will be placed around sunflower planting areas susceptible to high damage. This will lrclp WS ynsu~ult.l Cutiw heir eKurh un locating cattail-dominated wetlands that should be enrolled in the management program and improving current and fuhlre blackbird damage management programs

Site Use of European Starlings Wintering in Central New Jersey

Managing European starlings with DRC-1339 near urban and suburban areas can lead to adverse publicity resulting from encounters by the public with dead and dying birds. Collectors could retrieve the birds, if the likely sites of mass mortalities were known. In December 2009, we radio tagged 50 starlings at 3 sites in central New Jersey and studied their movements and behavior. Two of the sites were ensconced in a mosaic of suburban and urban habitats, whereas the other was in a rural setting. The sites were selected from a list of agricultural producers that had requested assistance from the Wildlife Services program in New Jersey. Starlings using the rural study site showed strong site fidelity (x = 78% of days tracked), stayed closer during daytime wanderings (x = 2 km), and roosted onsite. In contrast, starlings in the urban-suburban mosaic showed less fidelity (x’s = 10% and 36%), wandered farther (x’s = 6 km and 4 km), and seldom roosted onsite. No study sites were visited by members from the other radio-tagged cohorts. Major roosts in the urban-suburban mosaic averaged 10 km (n = 4, SE = 1.4) from the study sites. We predict that most starlings will remain within 6 km of the site during daytime. Poisoned starlings may become lethargic and seek refuge in dense vegetation (e.g., evergreens) near the baited site. Birds \u3e6 km from a bait site are probably on a direct bearing between the bait site and roosting site

Site Use of European Starlings Captured and Radio-Tagged at Texas Feedlots during Winter

We radio tagged and tracked 50 European starlings between December 2008 and January 2009 at 3 feedlots in the northern Texas Panhandle. Daily fidelity to sites of capture (home feedlots) was different among the 3 radio-tagged cohorts. Cohorts from Sites A and C were recorded at home feedlots on 48 and 59% of tracking days, respectively. The Site B cohort was at its home feedlot 95% of days. There were qualitative differences in use of home feedlots between cohorts A and C. The former were nearly obligate in their use of concentrated animal feeding operations (CAFO), whereas the latter tended to balance their use between CAFO and a nearby urban center. Six birds (12%) used either one or both of the counterpart home feedlots. Of these, 5 permanently switched from their home feedlots and used counterpart home feedlots; one bird captured at Site B alternated between Sites A and C after abandoning its home feedlot. Use of roost sites depended on habitat composition surrounding the study feedlots. Urban habitats were used as roosts by several birds from Sites A and C, whereas birds using Site B roosted at a petroleum refinery and a reservoir. Some Site B individuals used both roost sites during the study period; however, the reservoir was the preferred roost site. Daily activities in habitats away from the home feedlot generally occurred ≤5 km from the home feedlot. For birds from Sites A and C, offsite habitats were mainly urban areas and small CAFO. Increased habitat heterogeneity, as exemplified in our study by urban habitats and CAFO near Sites A and C, seemed to reduce rates of daily use of home feedlots. Heterogeneous environments can complicate management strategies that use DRC-1339 Concentrate for reducing starling numbers at infested CAFO. First, starlings may be erratic in their daily use of a CAFO in complex environments. Secondly, urban areas, when present, may be used as refuges by poisoned birds, leading to adverse public exposure