Potential and realized nutrient resorption in serpentine and non-serpentine chaparral shrubs and trees

Low-nutrient adapted species have numerous mechanisms that aid in nutrient conservation. Hypothetically, species adapted to nutrient-poor soils should have tighter internal nutrient recycling, as evidenced by greater resorption. However, literature results are mixed. We suggest methodological factors may limit our understanding of this process. We hypothesized that plants adapted to serpentine soils would be more proficient in resorbing N and P than plants adapted to non-serpentine soils, although there would be differences among functional groups within each soil type. For six growing seasons, we sampled senescent leaf tissue from the dominant and co-dominant shrubs and trees found in serpentine and non-serpentine chaparral communities in the California Coast Range. Our study also explicitly included congener pairs found on both soil types. Most species were highly N proficient, but species adapted to serpentine soils were more P proficient. Surprisingly, two of the three potential N-fixing species were also highly N proficient. Evergreen Quercus congeners were more N proficient than their deciduous congener pairs, although there was no difference in P resorption proficiency. Overall, large inter-annual variation was observed among most species sampled, but at least in some years, maximum potential resorption likely was reached. However, climate (temperature and precipitation) was not strongly correlated with either N or P resorption proficiency. Our data suggest that controlling for phylogeny can aid in interpretation of resorption patterns. More importantly, our study clearly shows that resorption patterns can only be discerned through long-term datasets, of which few exist in the literature

MECHANISMS OF SEXUAL MODE EVOLUTION IN CAENORHABDITIS ELEGANS

ABSTRACT Title of dissertation: MECHANISMS OF SEXUAL MODE EVOLUTION IN CAENORHABDITIS ELEGANS Lauren Skelly, Doctor of Philosophy, 2022 Dissertation directed by: Professor Eric S. Haag, Department of Biology The evolution of phenotypic novelties is a broad biological phenomenon, and how organisms evolve new traits is dependent on the molecular mechanisms that underlie those traits. Transcriptional regulation is often the focus of phenotype evolution, but post-transcriptional mechanisms such as mRNA splicing, stability, and translational control are also important components. Germ cells are particularly influenced by post-transcriptional mechanisms and are therefore a good system to study how these mechanisms lead to phenotypic novelties. This topic can be studied especially well in model systems that contain closely related species with recently evolved traits, such as self-fertility in Caenorhabditis elegans hermaphrodites. C. elegans hermaphrodites are essentially XX females that evolved the ability to produce sperm in an ovary. There are many known components necessary for sperm development, including a protein-protein-mRNA complex consisting of GLD-1, FOG-2 and tra-2. GLD-1 and FOG-2 dimerize and bind the 3’ UTR of tra-2 mRNA to repress its activity during spermatogenesis. In this work, I show FOG-2 directly interacts with TRA-2 protein, leading to the model that FOG-2 targets TRA-2 protein for degradation during its translation allowing sperm to form. I also show the expression pattern of tra-2 mRNA in the germline, and that GLD-1 binding on the 3’ UTR does not influence its localization. Another method for the study of novel traits is through hybridization of closely related species. In this work, I attempt to hybridize two closely related species of Caenorhabditis, another self-fertile species C. briggsae and an outcrossing species C. nigoni, to map the genetic loci underlying self-fertility. These hybrid crosses are unable to map genetic loci because males are inviable. These results agree with previous studies. This work contributes to the study of phenotypic evolution by adding an underlying molecular mechanism to Caenorhabditis sex determination

Computer recommendations for an automatic approach and landing system for V/STOL aircraft. Volume 2 - Equations

Automatic approach and landing system for V/STOL aircraf

Ozone exposure, uptake, and response of different-sized black cherry trees

Differences in exposure, uptake and relative sensitivity to ozone between seedling, sapling, and canopy black cherry (Prunus serotina Ehrh.) trees were characterized during two growing seasons in north central Pennsylvania. Open-grown trees of all sizes received a similar amount of ozone exposure. Seedlings had greater foliar ozone injury, expressed as adaxial stipple and early leaf senescence, than larger trees, which was correlated with their higher rates of stomatal conductance and greater rates of ozone uptake. The higher stomatal conductance and ozone uptake of seedlings was proportional to their higher (less negative) predawn xylem water potentials. Seedlings appeared to have some ability to compensate for injury because their free growth habit reduced exposure per unit leaf area compared to larger trees whose leaves were exposed to ozone throughout the entire growing season

Impact of Baseline Magnetic Resonance Imaging on Neurologic, Functional, and Safety Outcomes in Patients With Acute Traumatic Spinal Cord Injury

Study Design: Systematic review. Objective: To perform a systematic review to evaluate the utility of magnetic resonance imaging (MRI) in patients with acute spinal cord injury (SCI). Methods: An electronic search of Medline, EMBASE, the Cochrane Collaboration Library, and Google Scholar was conducted for literature published through May 12, 2015, to answer key questions associated with the use of MRI in patients with acute SCI. Results: The literature search yielded 796 potentially relevant citations, 8 of which were included in this review. One study used MRI in a protocol to decide on early surgical decompression. The MRI-protocol group showed improved outcomes; however, the quality of evidence was deemed very low due to selection bias. Seven studies reported MRI predictors of neurologic or functional outcomes. There was moderate-quality evidence that longer intramedullary hemorrhage (2 studies) and low-quality evidence that smaller spinal canal diameter at the location of maximal spinal cord compression and the presence of cord swelling are associated with poor neurologic recovery. There was moderate-quality evidence that clinical outcomes are not predicted by SCI lesion length and the presence of cord edema. Conclusions: Certain MRI characteristics appear to be predictive of outcomes in acute SCI, including length of intramedullary hemorrhage (moderate-quality evidence), canal diameter at maximal spinal cord compression (low-quality evidence), and spinal cord swelling (low-quality evidence). Other imaging features were either inconsistently (presence of hemorrhage, maximal canal compromise, and edema length) or not associated with outcomes. The paucity of literature highlights the need for well-designed prospective studies. © 2017, © The Author(s) 2017

Variable X-ray emission from the accretion shock in the classical T Tauri star V2129 Ophiuchi

Context. The soft X-ray emission from high density plasma observed in several CTTS is usually associated with the accretion process. However, it is still unclear whether this high density “cool” plasma is heated in the accretion shock, or if it is coronal plasma fed or modified by the accretion process. Aims. We conducted a coordinated quasi-simultaneous optical and X-ray observing campaign of the CTTS V2129 Oph. In this paper, we analyze Chandra grating spectrometer data and attempt to correlate the observed X-ray emitting plasma components with the characteristics of the accretion process and the stellar magnetic field constrained by simultaneous optical observations. Methods. We analyze a 200 ks Chandra/HETGS observation, subdivided into two 100 ks segments, of the CTTS V2129 Oph. For the two observing segments corresponding to two different phases within one stellar rotation, we measure the density of the cool plasma component and the emission measure distribution. Results. The X-ray emitting plasma covers a wide range of temperatures: from 2 up to 34 MK. The cool plasma component of V2129 Oph (T ≈ 3−4 MK) varies between the two segments of the Chandra observation: during the first observing segment high density plasma (log N_c = 12.1_(-1.1)^(+0.6)) with high EM at ~3−4 MK is present, whereas, during the second segment, this plasma component has lower EM and lower density (log N_e 3 R_⋆). Conclusions. Our observation provides additional confirmation that the dense cool plasma at a few MK in CTTS is material heated in the accretion shock. The variability of this cool plasma component on V2129 Oph may be explained in terms of X-rays emitted in the accretion shock and seen with different viewing angles at the two rotational phases probed by our observation. In particular, during the first time interval a direct view of the shock region is possible, while, during the second, the accretion funnel itself intersects the line of sight to the shock region, preventing us from observing the accretion-driven X-rays

Non-stationary dynamo & magnetospheric accretion processes of the classical T Tauri star V2129 Oph

We report here the first results of a multi-wavelength campaign focussing on magnetospheric accretion processes of the classical TTauri star (cTTS) V2129Oph. In this paper, we present spectropolarimetric observations collected in 2009 July with ESPaDOnS at the Canada-France-Hawaii Telescope (CFHT). Circularly polarised Zeeman signatures are clearly detected, both in photospheric absorption and accretion-powered emission lines, from time-series of which we reconstruct new maps of the magnetic field, photospheric brightness and accretion-powered emission at the surface of V2129Oph using our newest tomographic imaging tool - to be compared with those derived from our old 2005 June data set, reanalyzed in the exact same way. We find that in 2009 July, V2129Oph hosts octupolar & dipolar field components of about 2.1 & 0.9kG respectively, both tilted by about 20deg with respect to the rotation axis; we conclude that the large-scale magnetic topology changed significantly since 2005 June (when the octupole and dipole components were about 1.5 and 3 times weaker respectively), demonstrating that the field of V2129Oph is generated by a non-stationary dynamo. We also show that V2129Oph features a dark photospheric spot and a localised area of accretion-powered emission, both close to the main surface magnetic region (hosting fields of up to about 4kG in 2009 July). We finally obtain that the surface shear of V2129Oph is about half as strong as solar. From the fluxes of accretion-powered emission lines, we estimate that the observed average logarithmic accretion rate (in Msun/yr) at the surface of V2129Oph is -9.2+-0.3 at both epochs, peaking at -9.0 at magnetic maximum. It implies in particular that the radius at which the magnetic field of V2129Oph truncates the inner accretion disc is 0.93x and 0.50x the corotation radius in 2009 July and 2005 June respectively.Comment: MNRAS in press - 16 pages, 9 figure

The Cost of Blindness in the Republic of Ireland 2010-2020

Aims. To estimate the prevalence of blindness in the Republic of Ireland and the associated financial and total economic cost between 2010 and 2020. Methods. Estimates for the prevalence of blindness in the Republic of Ireland were based on blindness registration data from the National Council for the Blind of Ireland. Estimates for the financial and total economic cost of blindness were based on the sum of direct and indirect healthcare and nonhealthcare costs. Results. We estimate that there were 12,995 blind individuals in Ireland in 2010 and in 2020 there will be 17,997. We estimate that the financial and total economic costs of blindness in the Republic of Ireland in 2010 were €276.6 million and €809 million, respectively, and will increase in 2020 to €367 million and €1.1 billion, respectively. Conclusions. Here, ninety-eight percent of the cost of blindness is borne by the Departments of Social Protection and Finance and not by the Department of Health as might initially be expected. Cost of illness studies should play a role in public policy making as they help to quantify the indirect or “hidden” costs of disability and so help to reveal the true cost of illness

Effectiveness of Webinars as Educational Tools to Address Horse Industry Issues

A series of six webinars was developed and presented as part of an eXtension HorseQuest/My Horse University online educational program funded by the USA Equestrian Trust. The webinars addressed topics consistent with the goal of improving equine health and management practices through dissemination to horse owners of relevant research findings on current horse industry issues. The webinars were presented and recorded and then archived to both eXtension.org/horses and myhorseuniversity.com. Data from a voluntary postwebinar survey showed that a viable tool was successfully created and used to disseminate current research-based information to the industry

Comparative landscape dynamics of two anuran species: climate‐driven interaction of local and regional processes

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/117162/1/ecm2009793503.pd