Intergenerational transmission of attachment. Family interactive dynamics and psychopathology: what kind of relationship in adolescence?

Introduction: this work is an example of empirical research. The aim was to look to the possible transgenerational influence between parents and adolescents attachment bond to their respective parents, infant armonic and/or disarmonic development and functional or dysfunctional family interactions. Methodology: 40 families with adolescents aged from 12 to 18 years (μ = 14.575, σ = 1.716) coming for a psychodiagnostic evaluation were tested with Lausanne Trilogue Play, Parental Bonding Instrument, Child Behaviour Checklist and Youth Self Report. Hypothesis: a) is there an association between the adolescent’s perceived attachment relationship with his parents and his psychopathological symptoms? In this case a non parametric test for k independent groups was performed. b) is there an association between parents-adolescent interactive dynamics and the parents’ perceived attachment relationship with their parents (adolescent’s grand-parents). In this case correlations and non-parametric test for k independent groups were performed. Results: a) we found significant statistical differences (p < .05) between adolescent psychopathology and the quality of perceived relationship with both the mother and the father. b) we found positive correlations between quality of relationship between the mother and her father (adolescent grandfather) and the scores of some LTP scales concerning normative function; moreover we found negative correlations between the father and his mother (adolescent’s grandmother) and the scores of some LTP scales concerning affective function. Conclusion: these results underline a significant association between the internal working model of the mother and her ways to interact and manage the relation with her adolescent son; this is a clinical evidence too. Another relevant result is the association between adolescent’s psychopathology and his internal working model. Clinical applications regarding these findings should be taken in account when psychotherapeutically working with adolescents and their families

Emotional Difficulties in Adolescence: Psychopathology and Family Interactions.

Introduction. Several studies on developmental age have investigated aspects relating to emotional competence, and alexithymia in particular, showing that it is associated with behavioral problems in childhood and adolescence. Some such research has focused on the relationship between emotional difficulties and family interactions assuming a link between the quality of family relationships and a child\u2019s emotional competence. Subjects and Methods. The aims of the present study were: 1) to compare a group of psychiatric adolescents with a group of \u201chealthy\u201d adolescents in terms of any alexithymia and its relationship with the former\u2019s psychopathological issues; 2) to clarify the relationship, if any, between psychopathology, alexithymia and family interaction patterns in our sample of psychiatric adolescents. The experimental group consisted of 41 psychiatric adolescents and the control group of 41 students matched for gender and age. The Toronto Alexithymia Scale (TAS-20) was used to identify any alexithymic traits, the Youth Self Report (YSR) 11-18 and the CBCL to detect any psycho-behavioral problems, and the Lausanne Trilogue Play (LTP) to analyze family interactions. Results. There was a higher prevalence of alexithymia among the adolescents with mental health problems than in the control group, and a correlation between their scores for internalizing problems and alexithymia. In the experimental group, adolescents with internalizing problems, somatic complaints and attention difficulties belonged to families revealing high levels of parental conflict. As for alexithymia, adolescents\u2019 difficulty identifying emotions correlated significantly with the same trait in their mothers. This feature also seemed to be associated with better family interactions. Conclusion. Our study confirms the importance of family relationships in the development of emotional skills, and highlights how deficiencies in the development of emotional competence are strongly associated with psychopathologies in adolescence. In the light of these findings, it is advisable in clinical practice to provide psychotherapeutic interventions for teens and their parents

Biocomposites based on poly(butylene succinate) and curaua: Mechanical and morphological properties

Abstract Biocomposites based on poly(butylene succinate) (PBS) and curaua fibers have been produced by compression molding, and investigated as a function of fiber length and amount. Mechanical tests, water uptake and morphology studies were carried out in order to assess the composite features according to the characteristics of the reinforcing agents. It turns out that the impact and flexural strengths increase with fiber content. Moreover, the fiber length, varying from 1 to 4 cm for the composite reinforced with 20 wt% of fiber, influences impact strength, which is higher for shorter than for longer fibers. However, flexural strength is not greatly influenced by the length of the fibers. Water uptake studies reveal a higher sensitivity of the material to fiber content rather than fiber size. Biocomposites, which are characterized by enhanced mechanical properties as compared to PBS, can have different applications, for example in rigid packaging or interior car parts

An investigation on the possible use of coffee silverskin in PLA/PBS composites

The production of degradable packaging materials is a task that can be no lon-ger postponed. Moreover, high amounts of agricultural wastes are landfilledwithout any recycling. In this research, the possibility to formulate particulatecomposites made of biopolymers filled with coffee waste with acceptable physi-cal and mechanical characteristics that will degrade is investigated. The addi-tion of this agricultural waste, by reducing the requested amount ofbiopolymer, can decrease the overall price of the material presently the mainlimiting factor to the use of biopolymers in the packaging industry. Silverskin,the integument of coffee beans discarded during the roasting process, after amilling step, is added up to a 30 wt% either to polylactic acid (PLA) or to ablend of PLA and polybutylene succinate. The filler can be homogeneously dis-persed in both systems. The data shows that the silverskin filler increases theelastic modulus but decreases the tensile strength of the material and helps thedevelopment of crystal phase in the matrix. The thermal stability and thehydrophobicity of the materials stay almost unchanged on filler addition.Moreover, data shows that the addition of silverskin increases the materialssusceptibility to microbial attack

Evaluation of the activity of natural phenolic antioxidants, extracted from industrial coffee residues, on the stability of poly(1,4‐butylene succinate) formulations

In this work, the evaluation of the antioxidant activity of natural phenolic compounds is performed and compared to that of a conventional antioxidative agent. Phenolic molecules, extracted from industrial processing coffee residues, are added to a matrix of poly(1,4-butylene succinate) (PBS). The apparent activation energy (Ea) of the thermo-oxidative degradation is calculated by employing different methods like Kissinger-Akahira-Sunose, Flynn-Wall-Ozawa and Friedman. The results are compared with the antioxidant activity evaluation obtained through the ABTS radical scavenging assay. From the average activation energies, it is observed that the addition of the natural antioxidants led to an increase in the activation energy of the degradation process as a function of the phenolic compound content. This trend is confirmed by the results of the ABTS assay. Hence, this study proves that the active molecules extracted from agri-food waste could be employed to improve the antioxidant capacity of the biopolymer, even if the composition of the extract must be evaluated in order to mitigate the effects of other components

Memory effect in melting behaviour, crystallization kinetics and morphology of poly(propylene terephthalate)

AbstractCrystallization kinetics and melting behaviour of poly(propylene terephthalate) (PPT) were investigated by means of differential scanning calorimetry and hot-stage optical microscopy. Isothermal crystallization kinetics was analysed according to the Avrami treatment. The effects of temperature and duration of melting on the overall rate of isothermal crystallization were studied: the rate was found to decrease with increasing melting temperature and melting time. This result was discussed on the basis of the gradual destruction of predetermined athermal nuclei. Values of the Avrami exponent close to 3 were obtained, regardless of the adopted thermal treatment and the crystallization temperature, Tc, in agreement with a crystallization process originating from predetermined nuclei and characterized by three-dimensional spherulitic growth. As a matter of fact, spacefilling spherulites were observed by optical microscopy at all Tc's, independent of the applied thermal treatments. For each of them, the rate of crystallization became lower as Tc increased, as usual at low undercooling where the crystallization process is controlled by nucleation. The observed multiple endotherms, which are commonly displayed by polyesters, were influenced by Tc and ascribed to melting and recrystallization processes. Linear and non-linear treatments were applied in order to estimate the equilibrium melting temperature for PPT, by using the corrected melting temperatures. The non-linear estimation yielded an about 33°C higher value with respect to the one obtained by means of the linear approach. Through the analysis of secondary nucleation theory, the classical II→III transition was found to occur at a temperature of 194°C. The average work of chain folding for nucleation was determined to be c. 5.2 kcal/mol. The heat of fusion was correlated to the specific heat increment for samples with different degree of crystallinity and the results were interpreted on the basis of the existence of an interphase, whose amount was found to depend on the thermal treatment the polymer was subjected to

The Lausanne Trilogue Play within the outcome evaluation in infant mental health: a preliminary report

This study aims to contribute to the scientific debate about the evaluation of the intervention in infant mental health and presents the main results after one year of intervention based on integrated types of treatments (psychodynamic psychotherapy for the child/adolescent, parental support and observation and assessment of family interactions). Furthermore, the study aims to explore the use of the Lausanne Trilogue Play (LTP) as a new assessment tool for planning the of treatments. The sample consists of 23 children and adolescents, aged between 4 and 17 years old, and their families, attending the Infancy, Adolescence and Family Service, Local Health Unit 16 of Padua due to their psychopathological problems. In order to assess their psychological conditions we used the Child Behavior Checklist (CBCL) to evaluate psycho-behavioral problems and the LTP to assess family interactions. The CBCL was used at the time of the diagnostic assessment (T0) and after 12 months of treatment (T12). Concerning the application of LTP, the sample was divided randomly in two groups: one where LTP was used in two different time intervals during therapy (T0 and T12) and another one where it was used in three different time intervals during therapy (T0, T6 and T12) with a video-feedback intervention at time T6. Results report a favorable outcome in regards of the gravity of patients’ symptoms, displaying, after one year of treatment, a statistical significant decrease in the clinical level of internalizing symptoms. In terms of family interactions, results show stability within the family patterns except for a statistically significant deterioration in the management of interactive mistakes. Regarding the use of LTP as evaluator of the above intervention, the study shows that the assessment of family’s interactions during long-term psychotherapy helps clinicians focus the intervention on those aspects that remain dysfunctional. These results and the possible interpretations, however, emphasize the need for further studies on this topic

Bordetella pertussis Can Be Motile and Express Flagellum-Like Structures

ABSTRACT Bordetella bronchiseptica encodes and expresses a flagellar apparatus. In contrast, Bordetella pertussis, the causative agent of whooping cough, has historically been described as a nonmotile and nonflagellated organism. The previous statements that B. pertussis was a nonmotile organism were consistent with a stop codon located in the flagellar biosynthesis gene, flhA, discovered when the B. pertussis Tohama I genome was sequenced and analyzed by Parkhill et al. in 2003 (J. Parkhill, M. Sebaihia, A. Preston, L. D. Murphy, et al., Nat Genet, 35:32– 40, 2003, https://doi.org/10 .1038/ng1227). The stop codon has subsequently been found in all annotated genomes. Parkhill et al. also showed, however, that B. pertussis contains all genetic material required for flagellar synthesis and function. We and others have determined by various transcriptomic analyses that these flagellar genes are differentially regulated under a variety of B. pertussis growth conditions. In light of these data, we tested for B. pertussis motility and found that both laboratory-adapted strains and clinical isolates can be motile. Upon isolation of motile B. pertussis, we discovered flagellum-like structures on the surface of the bacteria. B. pertussis motility appears to occur primarily in the Bvg() phase, consistent with regulation present in B. bronchiseptica. Motility can also be induced by the presence of fetal bovine serum. These observations demonstrate that B. pertussis can express flagellum-like structures, and although it remains to be determined if B. pertussis expresses flagella during infection or if motility and/or flagella play roles during the cycle of infection and transmission, it is clear that these data warrant further investigation. IMPORTANCE This report provides evidence for motility and expression of flagella by B. pertussis, a bacterium that has been reported as nonmotile since it was first isolated and studied. As with B. bronchiseptica, B. pertussis cells can express and assemble a flagellum-like structure on their surface, which in other organisms has been implicated in several important processes that occur in vivo. The discovery that B. pertussis is motile raises many questions, including those regarding the mechanisms of regulation for flagellar gene and protein expression and, importantly, the role of flagella during infection. This novel observation provides a foundation for further study of Bordetella flagella and motility in the contexts of infection and transmission

From winery waste to bioactive compounds and new polymeric biocomposites: a contribution to the circular economy concept

The paper aims at optimising and validating possible routes toward the full valorisation of grape agrowaste to produce bioactive molecules and new materials. Starting from Merlot red pomace, phenol complex mixtures were successfully extracted by using two different approaches. Extracts obtained by solvent-based (SE) technique contained up to 46.9 gGAeq/kgDW of total phenols. Depending on the used solvent, the prevalence of compounds belonging to different phenol families was achieved. Pressurized liquid extraction (PLE) gave higher total phenol yields (up to 79 gGAeq/kgDW) but a lower range of extracted compounds. All liquid extracts exerted strong antioxidant properties. Moreover, both SE and PLE extraction solid residues were directly exploited (between 5 and 20% w/w) to prepare biocomposite materials by direct mixing via an eco-friendly approach with PHBV polymer. The final composites showed mechanical characteristics similar to PHVB matrix. The use of pomace residues in biocomposites could therefore bring both to the reduction of the cost of the final material, as a lower amount of costly PHBV is used. The present research demonstrated the full valorisation of grape pomace, an agrowaste produced every year in large amounts and having a significant environmental impact