ZnO nanopowder derived from brass ash: Sintering behavior and mechanical properties

The present investigation studied the recycling of zinc from brass ash which is a secondary product produced during the brass smelting process. A retiring cycle was devised to produce high-purity ZnO nanopowders. Recovery of > 90 wt% of the total zinc available was achieved after the calcination of brass ash at 700 °C and a multistage hydrometallurgical treatment at room temperature. ZnO powder produced by the developed method was analyzed by X-ray diffraction, transmission electron scanning microscopy, ICP-AES and BET analysis. The ZnO nanopowder obtained from the brass ash was well dispersed and the size of the individual particles was in the range of 30–50 nm. The purity of the powder was 99.83 wt%, and the surface area was about 30.5 m2/g. A relative density level of about 98.1% was reached with ZnO pellets sintered at 1300 °C

Long-term health and germline transmission in transgenic cattle following transposon-mediated gene transfer

Abstract Background Transposon-mediated, non-viral gene delivery is a powerful tool for generating stable cell lines and transgenic animals. However, as multi-copy insertion is the preferred integration pattern, there is the potential for uncontrolled changes in endogenous gene expression and detrimental effects in cells or animals. Our group has previously reported on the generation of several transgenic cattle by using microinjection of the Sleeping Beauty (SB) and PiggyBac (PB) transposons and seeks to explore the long-term effects of this technology on cattle. Results Transgenic cattle, one female (SNU-SB-1) and one male (SNU-PB-1), reached over 36 months of age with no significant health issues and normal blood parameters. The detection of transgene integration and fluorescent signal in oocytes and sperm suggested the capacity for germline transmission in both of the founder animals. After natural breeding, the founder transgenic cow delivered a male calf and secreted milk containing fluorescent transgenic proteins. The calf expressed green fluorescent protein in primary cells from ear skin, with no significant change in overall genomic stability and blood parameters. Three sites of transgene integration were identified by next-generation sequencing of the calf’s genome. Conclusions Overall, these data demonstrate that transposon-mediated transgenesis can be applied to cattle without being detrimental to their long-term genomic stability or general health. We further suggest that this technology may be usefully applied in other fields, such as the generation of transgenic animal models

Additional file 1: of Long-term health and germline transmission in transgenic cattle following transposon-mediated gene transfer

Raw data of blood analysis from transgenic cattle. (XLSX 15 kb

Additional file 3: of Long-term health and germline transmission in transgenic cattle following transposon-mediated gene transfer

Figure S2. Detection of the expression of YFP in milk from SNU-SB-1 by confocal microscopy. Images of milk from wild type cattle (left) and SNU-SB-1 (right) taken using a high-throughput confocal microscope. YFP: YFP field. (PNG 1685 kb

Additional file 4: of Long-term health and germline transmission in transgenic cattle following transposon-mediated gene transfer

Figure S3. 5′ junction sequence analysis of all integration sites in SNU-F1–1. Sequences showing the genome-to-transposon junctions in the genome of SNU-F1–1 and the integration of transgenes at TTAA sites. (PNG 465 kb

Additional file 7: of Long-term health and germline transmission in transgenic cattle following transposon-mediated gene transfer

Figure S5. Overview of genomic variation in SNU-F1–2. Reference chromosomes from bt1 to btX are denoted by colored boxes at the outer edge. Plots denoting copy number variation (CNV; black dot plots in the green area), coverage (green line plot in the green area) and SNP density (orange histogram in orange area) for the SNU-F1–2 genome are shown for each 10 kb window. (PNG 1244 kb

New loci and coding variants confer risk for age-related macular degeneration in East Asians

加齢黄斑変性の発症に関わるアジア人特有の遺伝子変異を発見. 京都大学プレスリリース. 2015-02-05.Updated 30 March 2015. [Corrigendum] doi:10.1038/ncomms7817Age-related macular degeneration (AMD) is a major cause of blindness, but presents differently in Europeans and Asians. Here, we perform a genome-wide and exome-wide association study on 2, 119 patients with exudative AMD and 5, 691 controls, with independent replication in 4, 226 patients and 10, 289 controls, all of East Asian descent, as part of The Genetics of AMD in Asians (GAMA) Consortium. We find a strong association between CETP Asp442Gly (rs2303790), an East Asian-specific mutation, and increased risk of AMD (odds ratio (OR)=1.70, P=5.60 × 10[-22]). The AMD risk allele (442Gly), known to protect from coronary heart disease, increases HDL cholesterol levels by 0.17mmoll-1 (P=5.82 × 10[-21]) in East Asians (n=7, 102). We also identify three novel AMD loci: C6orf223 Ala231Ala (OR=0.78, P=6.19 × 10[-18]), SLC44A4 Asp47Val (OR=1.27, P=1.08 × 10[-11]) and FGD6 Gln257Arg (OR=0.87, P=2.85 × 10[-8]). Our findings suggest that some of the genetic loci conferring AMD susceptibility in East Asians are shared with Europeans, yet AMD in East Asians may also have a distinct genetic signature