reference architecture and framework

M. Adorni, F. Arcelli, S. Bandini, L. Baresi, C. Batini, A. Bianchi, D. Bianchini, M. Brioschi, A. Caforio, A. Cali, P. Cappellari, C. Cappiello, T. Catarci, A. Corallo, V. De Antonellis, C. Franza, G. Giunta, A. Limonta, G. Lorenzo, P. Losi, A. Maurino, M. Melideo, D. Micucci, S. Modafferi, E. Mussi, L. Negri, C. Pandolfo, B. Pernici, P. Plebani, D. Ragazzi, C. Raibulet, M. Riva, N. Simeoni, C. Simone, G. Solazzo, F. Tisato, R. Torlone, G. Vizzari, and A. Zill

Thelytokous Parthenogenesis in the Fungus-Gardening Ant Mycocepurus smithii (Hymenoptera: Formicidae)

The general prevalence of sexual reproduction over asexual reproduction among organisms testifies to the evolutionary benefits of recombination, such as accelerated adaptation to changing environments and elimination of deleterious mutations. Documented instances of asexual reproduction in groups otherwise dominated by sexual reproduction challenge evolutionary biologists to understand the special circumstances that might confer an advantage to asexual reproductive strategies. Here we report one such instance of asexual reproduction in the ants. We present evidence for obligate thelytoky in the asexual fungus-gardening ant, Mycocepurus smithii, in which queens produce female offspring from unfertilized eggs, workers are sterile, and males appear to be completely absent. Obligate thelytoky is implicated by reproductive physiology of queens, lack of males, absence of mating behavior, and natural history observations. An obligate thelytoky hypothesis is further supported by the absence of evidence indicating sexual reproduction or genetic recombination across the species' extensive distribution range (Mexico-Argentina). Potential conflicting evidence for sexual reproduction in this species derives from three Mycocepurus males reported in the literature, previously regarded as possible males of M. smithii. However, we show here that these specimens represent males of the congeneric species M. obsoletus, and not males of M. smithii. Mycocepurus smithii is unique among ants and among eusocial Hymenoptera, in that males seem to be completely absent and only queens (and not workers) produce diploid offspring via thelytoky. Because colonies consisting only of females can be propagated consecutively in the laboratory, M. smithii could be an adequate study organism a) to test hypotheses of the population-genetic advantages and disadvantages of asexual reproduction in a social organism and b) inform kin conflict theory

Resolution Scalable Image Coding with Reversible Cellular Automata

In a resolution scalable image coding algorithm, a multiresolution representation of the data is often obtained using a linear filter bank. Reversible cellular automata have been recently proposed as simpler, nonlinear filter banks that produce a similar representation. The original image is decomposed into four subbands, such that one of them retains most of the features of the original image at a reduced scale. In this paper, we discuss the utilization of reversible cellular automata and arithmetic coding for scalable compression of binary and grayscale images. In the binary case, the proposed algorithm that uses simple local rules compares well with the JBIG compression standard, in particular for images where the foreground is made of a simple connected region. For complex images, more efficient local rules based upon the lifting principle have been designed. They provide compression performances very close to or even better than JBIG, depending upon the image characteristics. In the grayscale case, and in particular for smooth images such as depth maps, the proposed algorithm outperforms both the JBIG and the JPEG2000 standards under most coding conditions

Pterandra pyroidea: a case of pollination shift within Neotropical Malpighiaceae

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Background and Aims Most Neotropical species of Malpighiaceae produce floral fatty oils in calyx glands to attract pollinating oil-collecting bees, which depend on this resource for reproduction. This specialized type of pollination system tends to be lost in members of the family that occur outside the geographic distribution (e.g. Africa) of Neotropical oil-collecting bees. This study focused on the pollination ecology, chemical ecology and reproductive biology of an oil flower species, Pterandra pyroidea (Malpighiaceae) from the Brazilian Cerrado. Populations of this species consist of plants with oil-secreting (glandular) flowers, plants with non-oil-secreting flowers (eglandular) or a mix of both plant types. This study specifically aims to clarify the role of eglandular morphs in this species. Methods Data on pollinators were recorded by in situ observations. Breeding system experiments were conducted by isolating inflorescences and by enzymatic reactions. Floral resources, pollen and floral oils offered by this species were analysed by staining and a combination of various spectroscopic methods. Key Results Eglandular flowers of P. pyroidea do not act as mimics of their oil-producing conspecifics to attract pollinators. Instead, both oil-producing and oil-free flowers depend on pollen-collecting bees for reproduction, and their main pollinators are bumble-bees. Floral oils produced by glandular flowers are less complex than those described in closely related genera. Conclusions Eglandular flowers represent a shift in the pollination system in which oil is being lost and pollen is becoming the main reward of P. pyroidea flowers. Pollination shifts of this kind have hitherto not been demonstrated empirically within Neotropical Malpighiaceae and this species exhibits an unusual transition from a specialized towards a generalized pollination system in an area considered the hotspot of oil-collecting bee diversity in the Neotropics. Transitions of this type provide an opportunity to study ongoing evolutionary mechanisms that promote the persistence of species previously involved in specialized mutualistic relationships.107813231334Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)NSF [DEB-0909511]D. Bennett Memorial Fellowship Fund (UT Austin)Lundell Chair endowment (UT Austin)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)NSF [DEB-0909511

Blocking p38/ERK crosstalk affects colorectal cancer growth by inducing apoptosis in vitro and in preclinical mouse models

We recently demonstrated that p38α is required to maintain colorectal cancer (CRC) metabolism, as its inhibition leads to FoxO3A activation, autophagy, cell death, and tumor growth reduction both in vitro and in vivo. Here we show that inhibition of p38α is followed by TRAIL-mediated activation of caspase-8 and FoxO3A-dependent HER3 upregulation with consequent overactivation of the MEK-ERK1/2 survival pathway. p38α and MEK combined inhibition specifically induces apoptosis by enabling TRAIL signaling propagation through t-Bid and caspase-3, and fosters cell death in CRC cells and preclinical mouse models. Current MEK1-directed pharmacological strategies could thus be exploited, in combination with p38α inhibition, to develop new approaches for CRC treatment

Blocking p38/ERK crosstalk affects colorectal cancer growth by inducing apoptosis in vitro and in preclinical mouse models

We recently demonstrated that p38α is required to maintain colorectal cancer (CRC) metabolism, as its inhibition leads to FoxO3A activation, autophagy, cell death, and tumor growth reduction both in vitro and in vivo. Here we show that inhibition of p38α is followed by TRAIL-mediated activation of caspase-8 and FoxO3A-dependent HER3 upregulation with consequent overactivation of the MEK-ERK1/2 survival pathway. p38α and MEK combined inhibition specifically induces apoptosis by enabling TRAIL signaling propagation through t-Bid and caspase-3, and fosters cell death in CRC cells and preclinical mouse models. Current MEK1-directed pharmacological strategies could thus be exploited, in combination with p38α inhibition, to develop new approaches for CRC treatment. © 2012 Elsevier Ireland Ltd

Reference Architecture and Framework

Mobile devices allow users to access information resources and services over many different distribution channels – anywhere, anytime, anyhow. Technical and usage characteristics of mobile systems are highly variable with respect to user capabilities and context characteristics. Therefore an immense level of flexibility is required. This book presents a framework for mobile information systems, focusing on quality of service and adaptability at all architectural levels. These levels range from adaptive applications to e-services, middleware, and infrastructural elements, as developed in the "Multichannel Adaptive Information Systems" (MAIS) project. The design models, methods, and tools developed in the project allow the realization of adaptive mobile information systems in a variety of different architectures. In particular, this chapter presents the general architecture of the MAIS project and the reference framework, which provides the essential basis for all the adaptive mechanisms that are illustrated in the book