Constitutive expression of clathrin hub hinders elicitor-induced clathrin-mediated endocytosis and defense gene expression in plant cells

AbstractEndocytosis has been recently implicated in the signaling network associated with the recognition of microbes by plants. In a previous study, we showed that the elicitor cryptogein was able to induce clathrin-mediated endocytosis (CME) in tobacco suspension cells. Herein, we investigate further the induced CME by means of a GFP-tagged clathrin light chain and a CME inhibitor, the hub domain of clathrin heavy chain. Hub constitutive expression does affect neither cell growth nor constitutive endocytosis but abolishes cryptogein-induced CME. Such an inhibition has no impact on early events in the cryptogein signaling pathway but reduces the expression of defense-associated genes

Observations of gas flows inside a protoplanetary gap

Gaseous giant planet formation is thought to occur in the first few million years following stellar birth. Models predict that giant planet formation carves a deep gap in the dust component (shallower in the gas). Infrared observations of the disk around the young star HD142527, at ~140pc, found an inner disk ~10AU in radius, surrounded by a particularly large gap, with a disrupted outer disk beyond 140AU, indicative of a perturbing planetary-mass body at ~90 AU. From radio observations, the bulk mass is molecular and lies in the outer disk, whose continuum emission has a horseshoe morphology. The vigorous stellar accretion rate would deplete the inner disk in less than a year, so in order to sustain the observed accretion, matter must flow from the outer-disk into the cavity and cross the gap. In dynamical models, the putative protoplanets channel outer-disk material into gap-crossing bridges that feed stellar accretion through the inner disk. Here we report observations with the Atacama Large Millimetre Array (ALMA) that reveal diffuse CO gas inside the gap, with denser HCO+ gas along gap-crossing filaments, and that confirm the horseshoe morphology of the outer disk. The estimated flow rate of the gas is in the range 7E-9 to 2E-7 Msun/yr, which is sufficient to maintain accretion onto the star at the present rate

Cercospora beticola toxins. Part XVII. The role of beticolin/Mg2+ complexes in their biological activity. Study of plasma membrane H+-ATPase, vacuolar H+-PPase, alkaline and acid phosphatases

Beticolin-1 and beticolin-2, yellow toxins produced by the phytopathogenic fungus Cercospora beticola, inhibit the plasma membrane H+-ATPase. Firstly, since beticolins are able to form complexes with Mg2+, the role of the beticolin/Mg2+ complexes in the inhibition of the plasma membrane proton pump has been investigated. Calculations indicate that beticolins could exist under several forms, in the H+-ATPase assay mixture, both free or complexed with Mg2+. However, the percentage inhibition of the H+-ATPase activity is correlated to the concentration of one single form of beticolin, the dimeric neutral complex Mg2H2B2, which appears to be the active form involved in the H+-ATPase inhibition. Secondly, since previous data suggested that beticolins could also be active against other Mg2+-dependent enzymes, we tested beticolin-1 on the vacoular H+-PPase, which requires Mg2+ as co-substrate, and on the alkaline and acid phosphatases, which do not use Mg2+ as co-substrate. Only vacuolar H+-PPase is sensitive to beticolin-1, which suggests that beticolins are specific to enzymes that use a complex of Mg2+ as the substrate. The same Mg2H2B2 complex which is responsible of the plasma membrane H+-ATPase inhibition appears to be also involved in the inhibition of the vacuolar H+-PPase

Warm molecular gas and kinematics in the disc around HD 100546

The disc around the Herbig Ae/Be star HD 100546 is one of the most extensively studied discs in the southern sky. Although there is a wealth of information about its dust content and composition, not much is known about its gas and large scale kinematics. We detect and study the molecular gas in the disc at spatial resolution from 7.7" to 18.9" using the APEX telescope. The lines 12CO J=7-6, J=6-5, J=3-2, 13CO J=3-2 and [C I] 3P2-3P1 are observed, diagnostic of disc temperature, size, chemistry, and kinematics. We use parametric disc models that reproduce the low-J 12CO emission from Herbig~Ae stars and vary the basic disc parameters - temperature, mass and size. Using the molecular excitation and radiative transfer code RATRAN we fit the observed spectral line profiles. Our observations are consistent with more than 0.001 Msun of molecular gas in a disc of approximately 400 AU radius in Keplerian rotation around a 2.5 Msun star, seen at an inclination of 50 degrees. The detected 12CO lines are dominated by gas at 30-70~K. The non-detection of the [C I] line indicates excess ultraviolet emission above that of a B9 type model stellar atmosphere. Asymmetry in the 12CO line emission suggests that one side of the outer disc is colder by 10-20~K than the other, possibly due to a shadow by a warped geometry of the inner disc. Pointing offsets, foreground cloud absorption and asymmetry in the disc extent are excluded scenarios. Efficient heating of the outer disc ensures that low- and high-J 12CO lines are dominated by the outermost disc regions, indicating a 400 AU radius. The 12CO J=6--5 line arises from a disc layer higher above disc midplane, and warmer by 15-20~K than the layer emitting the J=3--2 line. The existing models of discs around Herbig Ae stars, assuming a B9.5 type model stellar atmosphere overproduce the [CI] 3P2--3P1 line intensity from HD 100546 by an order of magnitude.Comment: 9pages, 3figures, Accepted for publication in Astronomy & Astrophysic

Biophysical analysis of the plant-specific GIPC sphingolipids reveals multiple modes of membrane regulation

The plant plasma membrane (PM) is an essential barrier between the cell and the external environment, controlling signal perception and transmission. It consists of an asymmetrical lipid bilayer made up of three different lipid classes: sphingolipids, sterols, and phospholipids. The glycosyl inositol phosphoryl ceramides (GIPCs), representing up to 40% of total sphingolipids, are assumed to be almost exclusively in the outer leaflet of the PM. However, their biological role and properties are poorly defined. In this study, we investigated the role of GIPCs in membrane organization. Because GIPCs are not commercially available, we developed a protocol to extract and isolate GIPC-enriched fractions from eudicots (cauliflower and tobacco) and monocots (leek and rice). Lipidomic analysis confirmed the presence of trihydroxylated long chain bases and 2-hydroxylated very long-chain fatty acids up to 26 carbon atoms. The glycan head groups of the GIPCs from monocots and dicots were analyzed by gas chromatograph–mass spectrometry, revealing different sugar moieties. Multiple biophysics tools, namely Langmuir monolayer, ζ-Potential, light scattering, neutron reflectivity, solid state 2H-NMR, and molecular modeling, were used to investigate the physical properties of the GIPCs, as well as their interaction with free and conjugated phytosterols. We showed that GIPCs increase the thickness and electronegativity of model membranes, interact differentially with the different phytosterols species, and regulate the gel-to-fluid phase transition during temperature variations. These results unveil the multiple roles played by GIPCs in the plant PM.Vers un modèle intégratif de la bicouche lipidique de la membrane plasmique végétaleDéveloppement d’une infrastructure française distribuée pour la métabolomique dédiée à l’innovatio

Multiword expressions at length and in depth: Extended papers from the MWE 2017 workshop

The annual workshop on multiword expressions takes place since 2001 in conjunction with major computational linguistics conferences and attracts the attention of an ever-growing community working on a variety of languages, linguistic phenomena and related computational processing issues. MWE 2017 took place in Valencia, Spain, and represented a vibrant panorama of the current research landscape on the computational treatment of multiword expressions, featuring many high-quality submissions. Furthermore, MWE 2017 included the first shared task on multilingual identification of verbal multiword expressions. The shared task, with extended communal work, has developed important multilingual resources and mobilised several research groups in computational linguistics worldwide. This book contains extended versions of selected papers from the workshop. Authors worked hard to include detailed explanations, broader and deeper analyses, and new exciting results, which were thoroughly reviewed by an internationally renowned committee. We hope that this distinctly joint effort will provide a meaningful and useful snapshot of the multilingual state of the art in multiword expressions modelling and processing, and will be a point point of reference for future work

Multiword expressions at length and in depth: Extended papers from the MWE 2017 workshop

The annual workshop on multiword expressions takes place since 2001 in conjunction with major computational linguistics conferences and attracts the attention of an ever-growing community working on a variety of languages, linguistic phenomena and related computational processing issues. MWE 2017 took place in Valencia, Spain, and represented a vibrant panorama of the current research landscape on the computational treatment of multiword expressions, featuring many high-quality submissions. Furthermore, MWE 2017 included the first shared task on multilingual identification of verbal multiword expressions. The shared task, with extended communal work, has developed important multilingual resources and mobilised several research groups in computational linguistics worldwide. This book contains extended versions of selected papers from the workshop. Authors worked hard to include detailed explanations, broader and deeper analyses, and new exciting results, which were thoroughly reviewed by an internationally renowned committee. We hope that this distinctly joint effort will provide a meaningful and useful snapshot of the multilingual state of the art in multiword expressions modelling and processing, and will be a point point of reference for future work

Multiword expressions at length and in depth: Extended papers from the MWE 2017 workshop

The annual workshop on multiword expressions takes place since 2001 in conjunction with major computational linguistics conferences and attracts the attention of an ever-growing community working on a variety of languages, linguistic phenomena and related computational processing issues. MWE 2017 took place in Valencia, Spain, and represented a vibrant panorama of the current research landscape on the computational treatment of multiword expressions, featuring many high-quality submissions. Furthermore, MWE 2017 included the first shared task on multilingual identification of verbal multiword expressions. The shared task, with extended communal work, has developed important multilingual resources and mobilised several research groups in computational linguistics worldwide. This book contains extended versions of selected papers from the workshop. Authors worked hard to include detailed explanations, broader and deeper analyses, and new exciting results, which were thoroughly reviewed by an internationally renowned committee. We hope that this distinctly joint effort will provide a meaningful and useful snapshot of the multilingual state of the art in multiword expressions modelling and processing, and will be a point point of reference for future work

Multiword expressions at length and in depth: Extended papers from the MWE 2017 workshop

The annual workshop on multiword expressions takes place since 2001 in conjunction with major computational linguistics conferences and attracts the attention of an ever-growing community working on a variety of languages, linguistic phenomena and related computational processing issues. MWE 2017 took place in Valencia, Spain, and represented a vibrant panorama of the current research landscape on the computational treatment of multiword expressions, featuring many high-quality submissions. Furthermore, MWE 2017 included the first shared task on multilingual identification of verbal multiword expressions. The shared task, with extended communal work, has developed important multilingual resources and mobilised several research groups in computational linguistics worldwide. This book contains extended versions of selected papers from the workshop. Authors worked hard to include detailed explanations, broader and deeper analyses, and new exciting results, which were thoroughly reviewed by an internationally renowned committee. We hope that this distinctly joint effort will provide a meaningful and useful snapshot of the multilingual state of the art in multiword expressions modelling and processing, and will be a point point of reference for future work