Effect of photodynamic therapy and non-thermal plasma on root canal filling: analysis of adhesion and sealer penetration

Objective The aim of this study was to evaluate the effect of photodynamic therapy (PDT) and non-thermal plasma (NTP) on adhesion and sealer penetration in root canals. Material and Methods Sixty single-rooted premolars were used. The teeth were prepared using a crown-down technique. NaOCl and EDTA were used for irrigation and smear layer removal, respectively. The root canals were divided into three groups: control, PDT, and NTP. After treatments, the roots were filled using gutta-percha and either AH Plus (AHP) or MTA Fillapex (MTAF) sealers. Samples were sectioned at 4, 8, and 12 mm from the apex (1-mm slices)and analyzed by the push-out bond strength test (adhesion) and confocal laser scanning microscopy (sealer penetration). Data were statistically evaluated using Kruskal-Wallis, Dunn’s, and Spearman’s tests. Results Regarding AHP, bond strength was similar in the NTP group and in the control group, but significantly lower in the PDT group. As to MTAF, both therapies showed lower values than the control group. In the confocal analysis of AHP, maximum and mean penetration, and penetrated area were statistically higher in the control group than in the PDT and NTP groups. Penetrated perimeter was similar among groups. Regarding MTAF, all parameters yielded better results in the NTP than in the control group. The PDT and control groups showed similar results except for penetrated area. Conclusion PDT and plasma therapy affected the adhesion and sealer penetration of root canals filled with AH Plus and MTA Fillapex and there is no positive correlation between adhesion and sealer penetration

A microleakage study of gutta-percha/AH Plus and Resilon/Real self-etch systems after different irrigation protocols

The development and maintenance of the sealing of the root canal system is the key to the success of root canal treatment. The resin-based adhesive material has the potential to reduce the microleakage of the root canal because of its adhesive properties and penetration into dentinal walls. Moreover, the irrigation protocols may have an influence on the adhesiveness of resin-based sealers to root dentin. Objective: The objective of the present study was to evaluate the effect of different irrigant protocols on coronal bacterial microleakage of gutta-percha/AH Plus and Resilon/Real Seal Self-etch systems. Material and Methods: One hundred ninety pre-molars were used. The teeth were divided into 18 experimental groups according to the irrigation protocols and filling materials used. The protocols used were: distilled water; sodium hypochlorite (NaOCl)+eDTA; NaOCl+H3PO4; NaOCl+eDTA+chlorhexidine (CHX); NaOCl+H3PO4+CHX; CHX+eDTA; CHX+ H3PO4; CHX+eDTA+CHX and CHX+H3PO4+CHX. Gutta-percha/AH Plus or Resilon/Real Seal Se were used as root-filling materials. The coronal microleakage was evaluated for 90 days against Enterococcus faecalis. Data were statistically analyzed using Kaplan-Meier survival test, Kruskal-Wallis and Mann-Whitney tests. Results: No significant difference was verified in the groups using chlorhexidine or sodium hypochlorite during the chemo-mechanical preparation followed by eDTA or phosphoric acid for smear layer removal. The same results were found for filling materials. However, the statistical analyses revealed that a final flush with 2% chlorhexidine reduced significantly the coronal microleakage. Conclusion: A final flush with 2% chlorhexidine after smear layer removal reduces coronal microleakage of teeth filled with gutta-percha/AH Plus or Resilon/Real Seal SE

A Microleakage Study Of Gutta-percha/ah Plus And Resilon/real Self-etch Systems After Different Irrigation Protocols.

The development and maintenance of the sealing of the root canal system is the key to the success of root canal treatment. The resin-based adhesive material has the potential to reduce the microleakage of the root canal because of its adhesive properties and penetration into dentinal walls. Moreover, the irrigation protocols may have an influence on the adhesiveness of resin-based sealers to root dentin. The objective of the present study was to evaluate the effect of different irrigant protocols on coronal bacterial microleakage of gutta-percha/AH Plus and Resilon/Real Seal Self-etch systems. One hundred ninety pre-molars were used. The teeth were divided into 18 experimental groups according to the irrigation protocols and filling materials used. The protocols used were: distilled water; sodium hypochlorite (NaOCl)+eDTA; NaOCl+H3PO4; NaOCl+eDTA+chlorhexidine (CHX); NaOCl+H3PO4+CHX; CHX+eDTA; CHX+ H3PO4; CHX+eDTA+CHX and CHX+H3PO4+CHX. Gutta-percha/AH Plus or Resilon/Real Seal Se were used as root-filling materials. The coronal microleakage was evaluated for 90 days against Enterococcus faecalis. Data were statistically analyzed using Kaplan-Meier survival test, Kruskal-Wallis and Mann-Whitney tests. No significant difference was verified in the groups using chlorhexidine or sodium hypochlorite during the chemo-mechanical preparation followed by eDTA or phosphoric acid for smear layer removal. The same results were found for filling materials. However, the statistical analyses revealed that a final flush with 2% chlorhexidine reduced significantly the coronal microleakage. A final flush with 2% chlorhexidine after smear layer removal reduces coronal microleakage of teeth filled with gutta-percha/AH Plus or Resilon/Real Seal SE.22174-

A microleakage study of gutta-percha/AH Plus and Resilon/Real self-etch systems after different irrigation protocols

The development and maintenance of the sealing of the root canal system is the key to the success of root canal treatment. The resin-based adhesive material has the potential to reduce the microleakage of the root canal because of its adhesive properties and penetration into dentinal walls. Moreover, the irrigation protocols may have an influence on the adhesiveness of resin-based sealers to root dentin. Objective: The objective of the present study was to evaluate the effect of different irrigant protocols on coronal bacterial microleakage of gutta-percha/AH Plus and Resilon/Real Seal Self-Etch systems. Material and Methods: One hundred ninety pre-molars were used. The teeth were divided into 18 experimental groups according to the irrigation protocols and filling materials used. The protocols used were: distilled water; sodium hypochlorite (NaOCl)+EDTA; NaOCl+H3PO4; NaOCl+EDTA+chlorhexidine (CHX); NaOCl+H3PO4+CHX; CHX+EDTA; CHX+ H3PO4; CHX+EDTA+CHX and CHX+H3PO4+CHX. Gutta-percha/AH Plus or Resilon/Real Seal SE were used as root-filling materials. The coronal microleakage was evaluated for 90 days against Enterococcus faecalis. Data were statistically analyzed using Kaplan-Meier survival test, Kruskal-Wallis and Mann-Whitney tests. Results: No significant difference was verified in the groups using chlorhexidine or sodium hypochlorite during the chemo-mechanical preparation followed by EDTA or phosphoric acid for smear layer removal. The same results were found for filling materials. However, the statistical analyses revealed that a final flush with 2% chlorhexidine reduced significantly the coronal microleakage. Conclusion: A final flush with 2% chlorhexidine after smear layer removal reduces coronal microleakage of teeth filled with gutta-percha/AH Plus or Resilon/Real Seal SE223174179CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP302575/2009-0sem informação2009/53976-0; 2010/50817-

NOVO ENSINO MÉDIO NO ESTADO DE MINAS GERAIS : ARCABOUÇO LEGAL DA IMPLANTAÇÃO

A presente pesquisa tem por objetivo conhecer, numa sequência cronológica de publicações, as principais legislações que ensejaram na implantação do Novo Ensino Médio no estado de Minas Gerais. Para a construção do presente artigo foi realizada uma pesquisa documental de abordagem qualitativa, seguida de uma análise de conteúdo que utilizou como fonte legislações publicadas no período de setembro de 2016 a julho de 2022. O Novo Ensino Médio é uma Política Pública Educacional de reforma da última etapa da Educação Básica: o Ensino Médio. Iniciada por meio de Medida Provisória no ano de 2016, cada Estado da Federação ficou responsável por normatizar o funcionamento e organização em seu território. No estado de Minas Gerais, por meio da Secretaria de Estado da Educação, o marco legal para a implantação do Novo Ensino Médio foi por meio de Parecer do Conselho Estadual de Educação, que aprovou o Currículo Referência do Ensino Médio dentro das habilidades e competências definidas na Base Nacional Curricular Comum. Para melhor compreensão da reforma, este artigo apresenta os principais pontos de vinte e quatro legislações que alicerçaram o Novo Ensino Médio. Este estudo poderá subsidiar discussões e reflexões desta relevante política pública educacional. O desafio em alcançar o objetivo da reforma pode estar no alinhamento dos entes da federação e, ao mesmo tempo, nas variáveis pertinentes a cada território, haja visto a extensão, a complexidade e diversidade existente dentro de um mesmo estado, como é o caso de Minas Gerais

Tailoring surface properties of functionalized graphene papers aiming to enzyme immobilization

he use of enzymes as catalysts requires recovery and reuse to make the process viable. Enzymatic immobilization changes enzyme stability, activity, and specificity. It is very important to explore new substrates for immobilization with appropriate composition and structure to improve the efficiency of the immobilized enzymes. This work explores the use of two different graphene oxide papers, one produced by oxidation route (GO) and the other by electrochemical synthesis (EG), aiming for β-galactosidase immobilization. The chemical and structural properties of these two papers were characterized by Raman spectroscopy, X-ray photoelectron spectroscopy and X-ray diffraction. Atomic force microscopy images showed that EG paper ensured more efficient immobilization of the enzymes on the surface of the paper. Cyclic voltammetry was used to monitor the reaction of conversion of lactose to glucose in the free enzyme solution and graphene paper immobilized enzyme solutions. The cyclic voltammetry analysis showed that immobilized enzymes on GO paper showed an improvement in the activity of β-galactose when compared to free enzyme solution, as well as enzyme immobilized on a glassy carbon electrode

A Simple Approach for the Synthesis of Gold Nanoparticles Mediated by Layered Double Hydroxide

The present work introduces a new procedure to obtain gold nanoparticles (AuNPs). AuNPs (77–213 nm) were obtained in the absence of any classical reducing agents in a medium containing Mg2+/Al3+ layered double hydroxide (LDH) and N,N-dimethylformamide. XRD analysis showed the presence of crystalline phases of gold in the Au/LDH composite. The 2θ values of peaks corresponding to the LDH interlayer distance indicated that metallic NPs were deposited on the surface of the material. Furthermore, atomic force microscopy (AFM) analysis showed that AuNPs tend to agglomerate in a nonclassical halter-like shape

Composição e método de obtenção de resinas fenólicas e processo de revestimento para superfícies metálicas

DepositadaA presente invenção proporciona uma composição de resinas fenólicas e um método inovador de cura através da geração de Plasma. O referido método é aplicável na cura de uma resina fenólica anticorrosiva do tipo NOVOLAC, termoplástica, à base de LCC aplicada em uma superfície metálica de aço carbono. Não há interrupção do processo de cura e após essa etapa obtém-se um revestimento de elevada estabilidade térmica e com superfície mais rígida, oriundos de um processo de cura mais eficiente e com tecnologia limpa

Apresentação

Apresentação do número 64 dos Cadernos do IL pelos organizadores

Uso da geoestatística no mapeamento de herbivoria em espécies nativas em área de recuperação florestal urbana no sudeste paraense

The action of herbivores can affect the successful establishment of seedlings, and through precision forestry, it becomes possible to map and identify the spatial relationships that occur. The objective of the study was to evaluate the spatial distribution of herbivore attacks, regrowth capacity, and mortality. The characterization of the pattern of spatial variability of the variables was performed by geostatistical analysis, and the spatial dependence obtained through semivariogram fitting. Subarea 5 was the area with the highest occurrence of herbivory (55%), and the best fitting semivariogram model was the exponential model, with strong spatial dependence (15.08%). Considering the 5 sub-areas, the species with the highest incidence of herbivory (79.49%) was inga (Inga edulis Mart.), and the regrowth capacity of the plants was 1.44%. Herbivore attack did not affect the survival rate of the plants, and the mortality rate from various causes was 5.76%.La acción de los herbívoros puede afectar el éxito en el establecimiento de plántulas, a través de la silvicultura de precisión, es posible mapear e identificar las relaciones espaciales que ocurren. El objetivo del estudio fue evaluar la distribución espacial de los ataques de herbívoros, la capacidad de rebrote y la mortalidad. La caracterización del patrón de variabilidad espacial de las variables se realizó mediante análisis geoestadístico y la dependencia espacial se obtuvo mediante ajustes de semivariograma. La subárea 5 fue el área con mayor ocurrencia de herbivoría (55%), y el modelo de semivariograma que mejor se ajustó fue el exponencial, con fuerte dependencia espacial (15,08%). Considerando las 5 subáreas, la especie con mayor incidencia de herbivoría (79,49%) fue el ingá (Inga edulis Mart.), y la capacidad de rebrote de las plantas fue de 1,44%. El ataque de los herbívoros no afectó la tasa de supervivencia de las plantas, y la tasa de mortalidad por diversas causas fue del 5,76%.A ação de herbívoros pode afetar o sucesso no estabelecimento de mudas, e através da silvicultura de precisão, torna-se possível mapear e identificar as relações espaciais ocorrentes. O objetivo do estudo foi avaliar a distribuição espacial dos ataques de herbívoros, capacidade de rebrota e mortalidade. A caracterização do padrão da variabilidade espacial das variáveis foi realizada por meio de análise geoestatística, e a dependência espacial obtida através de ajustes de semivariogramas. A subárea 5 apresentou maior ocorrência de herbivoria (55%), e o modelo de semivariograma que melhor se ajustou foi o exponencial, com dependência espacial forte (15,08%). Considerando as 5 subáreas, a espécie com maior incidência de herbivoria (79,49%) foi o ingá (Inga edulis), e a capacidade de rebrota das plantas foi de 1,44%. O ataque de herbívoros não afetou a taxa de sobrevivência das plantas, e a taxa de mortalidade por causas diversas foi de 5,76%