Organizational Water Footprint to Support Decision Making: a Case Study for a German Technological Solutions Provider for the Plumbing Industry

With water scarcity representing an increasing threat to humans, the environment and the economy, companies are interested in exploring how their operations and supply chains affect water resources globally. To allow for systematically compiling the water footprint at the company level, the organizational water footprint method based on ISO 14046 and ISO/TS 14072 was developed. This paper presents the first complete organizational water scarcity footprint case study carried out for Neoperl GmbH, a German company that offers innovative solutions regarding drinking water for the plumbing industry. The cradle-to-gate assessment for one year includes, besides facility-based production activities, purchased materials, electricity and fuels, and supporting activities, such as company vehicles and infrastructure. Neoperl’s total freshwater consumption amounts to approximately 110,000 m3, 96% thereof being attributable to the supply chain, with freshwater consumption through purchased metals playing the predominant role. Metals (mainly stainless steel and brass) are major hotspots, also when considering the water scarcity-related local impacts resulting from freshwater consumption, which mainly affect China and Chile. These results can be used to improve the company’s supply chain water use in cooperation with internal and external stakeholders by means of, e.g., sustainable purchase strategies or eco-design options to substitute water intensive materials.BMBF, 02WGR1429, GROW - Verbundprojekt WELLE: Wasserfußabdruck für Unternehmen - Lokale Maßnahmen in Globalen WertschöpfungskettenDFG, 414044773, Open Access Publizieren 2019 - 2020 / Technische Universität Berli

The role of limbic structures in financial abilities of mild cognitive impairment patients

Mild Cognitive Impairment (MCI) patients experience problems in financial abilities that affect everyday functioning. To date, the neural correlates of decline in this domain are unclear. This study aims at examining the correlation between the pattern of brain atrophy of MCI patients and performance on financial abilities. Forty-four MCI patients and thirty-seven healthy controls underwent structural magnetic resonance imaging, and assessment of financial abilitiesby means of the Numerical Activities of Daily Living Financial battery (NADL-F). As compared to healthy controls, MCI patients showed impaired performance in three out of the seven domains assessed by NADL-F: Item purchase, percentage, and financial concepts. The patients\u2019 performance in the NADL-F correlated with memory, language, visuo-spatial, and abstract reasoning composite scores. The analysis also revealed that volumetric differences in the limbic structures significantly correlated with financial abilities in MCI. Specifically, the patients\u2019 performance in the NADL-F was correlated with atrophy in the left medial and lateral amygdala and the right anterior thalamic radiation. These findings suggest that completing daily financial tasks involves sub-cortical regions in MCI and presumably also the motivational and emotional processes associated to them. Involvement of altered limbic structures in MCI patients suggests that impairment in financial abilities may be related to emotional and reflexive processing deficits

SIL1 deficiency causes degenerative changes of peripheral nerves and neuromuscular junctions in fish, mice and human.

BACKGROUND: Marinesco-Sjögren Syndrome (MSS) is a rare neuromuscular condition caused by recessive mutations in the SIL1 gene resulting in the absence of functional SIL1 protein, a co-chaperone for the major ER chaperone, BiP. As BiP is decisive for proper protein processing, loss of SIL1 results in the accumulation of misshaped proteins. This accumulation likely damages and destroys cells in vulnerable tissues, leading to congenital cataracts, cerebellar ataxia, vacuolar myopathy and other MSS phenotypes. Whether the peripheral nervous system (PNS) is affected in MSS has not been conclusively shown. METHODS: To study PNS vulnerability in MSS, intramuscular nerves fibres from MSS patients and from SIL1-deficient mice (woozy) as well as sciatic nerves and neuromuscular junctions (NMJ) from these mice have been investigated via transmission electron microscopic and immunofluorescence studies accompanied by transcript studies and unbiased proteomic profiling. In addition, PNS and NMJ integrity were analyzed via immunofluorescence studies in an MSS-zebrafish model which has been generated for that purpose. RESULTS: Electron microscopy revealed morphological changes indicative of impaired autophagy and mitochondrial maintenance in distal axons and in Schwann cells. Moreover, changes of the morphology of NMJs as well as of transcripts encoding proteins important for NMJ function were detected in woozy mice. These findings were in line with a grossly abnormal structure of NMJs in SIL1-deficient zebrafish embryos. Proteome profiling of sciatic nerve specimens from woozy mice revealed altered levels of proteins implicated in neuronal maintenance suggesting the activation of compensatory mechanisms. CONCLUSION: Taken together, our combined data expand the spectrum of tissues affected by SIL1-loss and suggest that impaired neuromuscular transmission might be part of MSS pathophysiology

Aproveitamento de subproduto de agroindústrias do setor queijeiro para desenvolvimento de produtos alimentícios e redução de impacto ambiental

The uses of by-products of cheese industry is still not significant in Brazil, since about 15% of liquid whey is used as raw material in food industry technology. The liquid whey has excellent technological properties, making it an alternative to minimize environmental impacts, besides it enables the use of the same nutritional properties in the production of new food products or adding value to the existing ones. The present study aimed to use liquid whey as an ingredient in the formulation of ice cream, hard candies, ham and breads, as well as to evaluate the influence of it in technological characteristics of the products and to quantify the volume of whey used. In order to evaluate the use of liquid whey, the products were developed with partial or total replacement of milk or water by whey, being the substitution accompanied by the determination of centesimal composition, as well as evaluating the visual characteristics of each product. The results show that the preparation of the products under study with substitution of raw milk or water is viable, proving that using liquid whey is a technological possibility of using a by-product considered to be an environmental pollutant. It was concluded that the use of liquid whey in the food industry reduces the environmental impact, since there is a reduction in the volume of whey discharged into the environment.A utilização de subprodutos da indústria queijeira ainda não é significativa no Brasil, uma vez que cerca de 15% do soro de leite líquido é empregado como matéria-prima no setor tecnológico alimentício. O soro de leite líquido apresenta excelentes propriedades tecnológicas, tornando-se uma alternativa para minimizar o impacto ambiental, além de possibilitar o aproveitamento das propriedades nutritivas do mesmo ao ser utilizado na produção de novos produtos alimentícios ou na agregação de valor aos já existentes. O presente estudo teve como objetivos empregar soro de leite líquido como ingrediente na formulação de sorvete, balas duras, apresuntado e pães, avaliar a influência do mesmo nas características tecnológicas dos produtos e quantificar o volume de soro empregado nas mesmas. Para avaliar o emprego do soro de leite líquido, os produtos foram elaborados com substituição parcial ou total de leite ou água pelo mesmo, sendo a substituição acompanhada pela determinação da composição centesimal, bem como avaliação das características visuais de cada produto. Os resultados obtidos evidenciam que a elaboração dos produtos em estudo com substituição das matérias-primas leite ou água é viável, demonstrando ser o emprego do soro de leite líquido uma possibilidade tecnológica de aproveitamento de um subproduto considerado poluente ambiental. Concluiu-se que a utilização do soro de leite líquido na indústria alimentícia reduz o impacto ambiental, uma vez que há redução do volume de soro de leite despejado no meio ambiente

Hemoglobin mRNA Changes in the Frontal Cortex of Patients with Neurodegenerative Diseases

International audienceBackground: Hemoglobin is the major protein found in erythrocytes, where it acts as an oxygen carrier molecule. In recent years, its expression has been reported also in neurons and glial cells, although its role in brain tissue remains still unknown. Altered hemoglobin expression has been associated with various neurodegenerative disorders. Here, we investigated hemoglobin mRNA levels in brains of patients affected by variant, iatrogenic, and sporadic forms of Creutzfeldt-Jakob disease (vCJD, iCJD, sCJD, respectively) and in different genetic forms of prion diseases (gPrD) in comparison to Alzheimer's disease (AD) subjects and age-matched controls.Methods: Total RNA was obtained from the frontal cortex of vCJD (n = 20), iCJD (n = 11), sCJD (n = 23), gPrD (n = 30), and AD (n = 14) patients and age-matched controls (n = 30). RT-qPCR was performed for hemoglobin transcripts HBB and HBA1/2 using four reference genes for normalization. In addition, expression analysis of the specific erythrocyte marker ALAS2 was performed in order to account for blood contamination of the tissue samples. Hba1/2 and Hbb protein expression was then investigated with immunofluorescence and confocal microscope analysis.Results: We observed a significant up-regulation of HBA1/2 in vCJD brains together with a significant down-regulation of HBB in iCJD. In addition, while in sporadic and genetic forms of prion disease hemoglobin transcripts did not shown any alterations, both chains display a strong down-regulation in AD brains. These results were confirmed also at a protein level.Conclusions: These data indicate distinct hemoglobin transcriptional responses depending on the specific alterations occurring in different neurodegenerative diseases. In particular, the initial site of misfolding event (central nervous system vs. peripheral tissue)—together with specific molecular and conformational features of the pathological agent of the disease—seem to dictate the peculiar hemoglobin dysregulation found in prion and non-prion neurodegenerative disorders.In addition, these results suggest that gene expression of HBB and HBA1/2 in brain tissue is differentially affected by distinct prion and prion-like aggregating protein strains. Validation of these results in more accessible tissues could prompt the development of novel diagnostic tests for neurodegenerative disorders

Production of dust by massive stars at high redshift

The large amounts of dust detected in sub-millimeter galaxies and quasars at high redshift pose a challenge to galaxy formation models and theories of cosmic dust formation. At z > 6 only stars of relatively high mass (> 3 Msun) are sufficiently short-lived to be potential stellar sources of dust. This review is devoted to identifying and quantifying the most important stellar channels of rapid dust formation. We ascertain the dust production efficiency of stars in the mass range 3-40 Msun using both observed and theoretical dust yields of evolved massive stars and supernovae (SNe) and provide analytical expressions for the dust production efficiencies in various scenarios. We also address the strong sensitivity of the total dust productivity to the initial mass function. From simple considerations, we find that, in the early Universe, high-mass (> 3 Msun) asymptotic giant branch stars can only be dominant dust producers if SNe generate <~ 3 x 10^-3 Msun of dust whereas SNe prevail if they are more efficient. We address the challenges in inferring dust masses and star-formation rates from observations of high-redshift galaxies. We conclude that significant SN dust production at high redshift is likely required to reproduce current dust mass estimates, possibly coupled with rapid dust grain growth in the interstellar medium.Comment: 72 pages, 9 figures, 5 tables; to be published in The Astronomy and Astrophysics Revie