Analysis of Photopic and Melanopic Lighting in Teaching Environments

Daylight and lighting seem to be a key tool for people\u2019s well-being, however, there are no specific and agreed recommendations that address both photopic and melanopic aspects in educational environments. The present work analyzed melanopic light in four teaching environments considering photopic indoor lighting, daylight depending on the window orientation, location of the observer in the room, and their line of view. The façade direction, daylight at 11.00 a.m. for six months from October to March, and the characteristics of each classroom, such as reflectance of the surfaces, location of the luminaires and their spectral and spatial power distributions, or calculation points affecting the melanopic light reaching the corneal vertical plane of a hypothetical control observer were studied. For this evaluation, classrooms were experimentally treated and simulated using DialuxEvo software, and the computer-generated values resembled the experimental values. Once the study was performed, an improvement proposal, based on LED lighting, was made to optimize the classroom lighting considering the melanopic requirements, which we ensured that users who passed through these classrooms had an adequate amount light at any time of the day. Our results simplify to the greatest lighting projects and enable designers to carry out optimized evaluations of specific environments from both the photometric and circadian perspectives

Evaluation of Visual and Nonvisual Levels of Daylight from Spectral Power Distributions Considering Orientation and Seasonality

The evaluation of both visual and nonvisual effects from the spectral power distribution (SPD) of outdoor light is critical in lighting design. The dome-light SPD characteristically changes continuously depending on the seasonality, orientation, altitude of the sun or hour of the day. Traditional photopic parameters, such as the illuminance, luminance or correlated colour temperature (CCT), have been widely studied, but presently, there is no melanopic measurement or evaluation method. This article discusses the processes involved in establishing a simple method to determine the SPD of daylight and solar radiation over the skydome in a location to accurately account for the effects of both photopic and circadian levels around a location. Once per month for one year, natural daylight was spectrally measured in the city of Zaragoza (Spain); radiometric and photometric characteristics were analysed by season; and circadian effects were calculated in terms of standard parameters described by the Commission International de l\u2019Eclairage (CIE), factors recommended by normative and scientific backgrounds. Finally, we suggest that the best parameter is the melanopic versus photopic irradiance ratio, which achieves reliable results at simplifying and correlating calculations

Robust and scalable barcoding for massively parallel long‑read sequencing

Nucleic-acid barcoding is an enabling technique for many applications, but its use remains limited in emerging long-read sequencing technologies with intrinsically low raw accuracy. Here, we apply so-called NS-watermark barcodes, whose error correction capability was previously validated in silico, in a proof of concept where we synthesize 3840 NS-watermark barcodes and use them to asymmetrically tag and simultaneously sequence amplicons from two evolutionarily distant species (namely Bordetella pertussis and Drosophila mojavensis) on the ONT MinION platform. To our knowledge, this is the largest number of distinct, non-random tags ever sequenced in parallel and the frst report of microarray-based synthesis as a source for large oligonucleotide pools for barcoding. We recovered the identity of more than 86% of the barcodes, with a crosstalk rate of 0.17% (i.e., one misassignment every 584 reads). This falls in the range of the index hopping rate of established, highaccuracy Illumina sequencing, despite the increased number of tags and the relatively low accuracy of both microarray-based synthesis and long-read sequencing. The robustness of NS-watermark barcodes, together with their scalable design and compatibility with low-cost massive synthesis, makes them promising for present and future sequencing applications requiring massive labeling, such as long-read single-cell RNA-Seq.Fil: Ezpeleta, Joaquín. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas; Argentina.Fil: Labari, Ignacio Garcia. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas; Argentina.Fil: Bulacio, Pilar. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas; Argentina.Fil: Tapia, Elizabeth. Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas; Argentina.Fil: Ezpeleta, Joaquín. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina.Fil: Bulacio, Pilar. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina.Fil: Tapia, Elizabeth. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina.Fil: Villanova, Gabriela Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Lavista Llanos, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Villanova, Gabriela Vanina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio Mixto de Biotecnología Acuática. Centro Científico Tecnológico y Educativo Acuario del Río Paraná; Argentina.Fil: Posner, Victoria María. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio Mixto de Biotecnología Acuática. Centro Científico Tecnológico y Educativo Acuario del Río Paraná; Argentina.Fil: Arranz, Silvia Eda. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio Mixto de Biotecnología Acuática. Centro Científico Tecnológico y Educativo Acuario del Río Paraná; Argentina.Fil: Krsticevic, Flavia. The Hebrew University of Jerusalem. Robert H Smith Faculty of Agriculture, Food and Environment; Israel

Assessment of VINO filters for correcting redgreen Color Vision Deficiency

In our ongoing research on the effectiveness of different passive tools for aiding Color Vision Deficiency (CVD) subjects, we have analyzed the VINO 02 Amp Oxy-Iso glasses using two strategies: 1) 52 observers were studied using four color tests (recognition, arrangement, discrimination, and color-naming); 2) the spectral transmittance of the lenses were used to model the color appearance of natural scenes for different simulated CVD subjects. We have also compared VINO and EnChroma glasses. The spectral transmission of the VINO glasses significantly changed color appearance. This change would allow some CVD subjects, above all the deutan ones, to be able to pass recognition tests but not the arrangement tests. To sum up, our results support the hypothesis that glasses with filters are unable to effectively resolve the problems related to color vision deficiency.The Spanish State Agency of Research (AEI); the Ministry for Economy, Industry and Competitiveness (MIMECO) (Grant numbers FIS2017-89258-P and DPI 2015-64571-R); European Union FEDER (European Regional Development Funds)

HTLV-1 infection in solid organ transplant donors and recipients in Spain

HTLV-1 infection is a neglected disease, despite infecting 10-15 million people worldwide and severe illnesses develop in 10% of carriers lifelong. Acknowledging a greater risk for developing HTLV-1 associated illnesses due to immunosuppression, screening is being widely considered in the transplantation setting. Herein, we report the experience with universal HTLV testing of donors and recipients of solid organ transplants in a survey conducted in Spain. All hospitals belonging to the Spanish HTLV network were invited to participate in the study. Briefly, HTLV antibody screening was performed retrospectively in all specimens collected from solid organ donors and recipients attended since the year 2008. A total of 5751 individuals were tested for HTLV antibodies at 8 sites. Donors represented 2312 (42.2%), of whom 17 (0.3%) were living kidney donors. The remaining 3439 (59.8%) were recipients. Spaniards represented nearly 80%. Overall, 9 individuals (0.16%) were initially reactive for HTLV antibodies. Six were donors and 3 were recipients. Using confirmatory tests, HTLV-1 could be confirmed in only two donors, one Spaniard and another from Colombia. Both kidneys of the Spaniard were inadvertently transplanted. Subacute myelopathy developed within 1 year in one recipient. The second recipient seroconverted for HTLV-1 but the kidney had to be removed soon due to rejection. Immunosuppression was stopped and 3 years later the patient remains in dialysis but otherwise asymptomatic. The rate of HTLV-1 is low but not negligible in donors/recipients of solid organ transplants in Spain. Universal HTLV screening should be recommended in all donor and recipients of solid organ transplantation in Spain. Evidence is overwhelming for very high virus transmission and increased risk along with the rapid development of subacute myelopathy

Experimental study of differentially rotating supersonic plasma flows produced by aluminium wire array Z-pinches

A novel approach to cylindrical wire array z-pinches has been developed in order to create a rotating plasma flow analogous to astrophysical accretion discs. The method involves subjecting the wire array to a cusp magnetic field (B_r) to create converging off axis ablation streams to form a rotating flow. The rotation is sustained by the ram pressure of the ablation streams in a quasi-equilibrium state for approximately 150 ns. This corresponds to one full rotation of the plasma about the axis. The rotating plasma is supersonic with Mach number ~2 and a radially constant rotation velocity between 60 and 75 km/s; the angular velocity therefore has an r^-1 dependence and the flow is differential. A Thomson scattering diagnostic is used to measure the electron and ion temperatures as Te ~30 eV and Ti >55 eV and the ionisation of the plasma (Z) between 6 and 8. These parameters are used to calculate the Reynolds number (10^5 to 10^6) and magnetic Reynolds numbers (20 to 100) which are large enough for viscous and resistive effects to be negligible on the large scale of the flow. These are of sufficient magnitude for the experiment to be scalable to astrophysical accretion discs. Further more the Reynolds number for the experiment is large enough for shear instabilities to manifest in the plasma. Some evidence for this can be seen in XUV images and Thomson spectra which indicate the development of perturbations and vorticity within the flow. Predictions for the growth rate of the Kelvin Helmholtz instability, 12 to 40 ns, agree reasonably well with the observed perturbation growth of ~30 ns. It is also possible that shear instabilities are driving hydrodynamic turbulence. Turbulent heating of the plasma could explain the approximately 500 eV increase in the ion temperature observed from some Thomson spectra. Further work is required however to prove the existence of shear flows and turbulence within the experiments.Open Acces

Diverse Large HIV-1 Non-subtype B Clusters Are Spreading Among Men Who Have Sex With Men in Spain

In Western Europe, the HIV-1 epidemic among men who have sex with men (MSM) is dominated by subtype B. However, recently, other genetic forms have been reported to circulate in this population, as evidenced by their grouping in clusters predominantly comprising European individuals. Here we describe four large HIV-1 non-subtype B clusters spreading among MSM in Spain. Samples were collected in 9 regions. A pol fragment was amplified from plasma RNA or blood-extracted DNA. Phylogenetic analyses were performed via maximum likelihood, including database sequences of the same genetic forms as the identified clusters. Times and locations of the most recent common ancestors (MRCA) of clusters were estimated with a Bayesian method. Five large non-subtype B clusters associated with MSM were identified. The largest one, of F1 subtype, was reported previously. The other four were of CRF02_AG (CRF02_1; n = 115) and subtypes A1 (A1_1; n = 66), F1 (F1_3; n = 36), and C (C_7; n = 17). Most individuals belonging to them had been diagnosed of HIV-1 infection in the last 10 years. Each cluster comprised viruses from 3 to 8 Spanish regions and also comprised or was related to viruses from other countries: CRF02_1 comprised a Japanese subcluster and viruses from 8 other countries from Western Europe, Asia, and South America; A1_1 comprised viruses from Portugal, United Kingom, and United States, and was related to the A1 strain circulating in Greece, Albania and Cyprus; F1_3 was related to viruses from Romania; and C_7 comprised viruses from Portugal and was related to a virus from Mozambique. A subcluster within CRF02_1 was associated with heterosexual transmission. Near full-length genomes of each cluster were of uniform genetic form. Times of MRCAs of CRF02_1, A1_1, F1_3, and C_7 were estimated around 1986, 1989, 2013, and 1983, respectively. MRCA locations for CRF02_1 and A1_1 were uncertain (however initial expansions in Spain in Madrid and Vigo, respectively, were estimated) and were most probable in Bilbao, Spain, for F1_3 and Portugal for C_7. These results show that the HIV-1 epidemic among MSM in Spain is becoming increasingly diverse through the expansion of diverse non-subtype B clusters, comprising or related to viruses circulating in other countries

La iluminación en espacios docentes: análisis fotópico y melanópico

La iluminación artificial tiene un papel fundamental en la vida de las personas, actualmente se pasa gran parte del día en ambientes de interior utilizando este tipo de iluminación, ya que hay ocasiones en los que no puede aprovecharse la luz diurna. Es conocido que la exposición a la luz solar es favorable para el ritmo circadiano, haciendo que los niveles del estímulo circadiano sean más elevados por la mañana y vayan disminuyendo a lo largo del día. Debido al interés actual por los avances en prevención y salud ocular, la iluminación utilizada en espacios interiores debe cumplir determinadas recomendaciones para que el sistema circadiano no se vea alterado, evitando así problemas de insomnio, bajo rendimiento en el trabajo, depresión… En este estudio, se analiza tanto los efectos visuales como no visuales de la luz en cuatro espacios docentes con diferentes orientaciones. Se analiza cómo afecta a la iluminación la orientación de las ventanas, el estado del cielo, así como las características de cada aula (grado de reflexión de las superficies o localización de los puntos de cálculo). Para ello, se realizaron medidas experimentales desde el mes de octubre hasta el mes de marzo, y, a su vez, se simuló las aulas con el software Dialux Evo donde se ha conseguido que los valores simulados se asemejen a los valores experimentales. Para finalizar, se hace una propuesta de mejora para optimizar la iluminación de las aulas contemplando los requisitos Human-Centric Lighting (HCL), asegurando que los usuarios que pasen por esas aulas tengan la cantidad adecuada de iluminación a cualquier hora del día

Restriction of Mouth Opening, Reduction in Pressure Pain Thresholds and Activation of Myofascial Trigger Points in Mandibular and Cervical Regions after Root Canal Therapy: A Quasi-Experimental Study

Background: In daily clinical practice, patients often refer temporomandibular or cervical complaints after different oral procedures, especially in lengthy procedures that can result in iatrogenic postures or trauma that can affect the temporomandibular joint (TMJ). This study aimed to evaluate the clinical and functional changes of the temporomandibular joint (TMJ) and cervical region immediately after a session of root canal therapy. Methods: Twenty-nine subjects who received a session of root canal therapy were included. Clinical assessments included mouth opening, cervical mobility, pain intensity, pressure pain thresholds (PPTs), and myofascial trigger points (MTrPs) of the jaw and neck muscles. Results: After the intervention, a significant reduction in mouth opening (41.90 mm; SD = 6.21) was observed compared to baseline (46.28 mm; SD = 6.17) (p p p = 0.002–0.026) were demonstrated after the intervention. Conclusions: A session of root canal therapy can produce an immediate significant reduction in mouth opening, PPTs, and cervical mobility, and an increase in MTrPs. The risk can be higher if there is a previous TMJ limitation

Classification of SSH Anomalous Connections

The Secure Shell Protocol (SSH) is a well-known standard protocol for remote login and used as well for other secure network services over an insecure network. It is mainly used for remotely accessing shell accounts on Unix-liked operating systems to perform administrative tasks. For this reason, the SSH service has been for years an attractive target for attackers, aiming to guess root passwords performing dictionary attacks, or to directly exploit the service itself. To test the classification performance of different classifiers and combinations of them, this study gathers and analyze SSH data coming from a honeynet and then it is analysed by means of a wide range of classifiers. The high-rate classification results lead to positive conclusions about the identification of malicious SSH connections