Physicochemical and microbiological characteristics of Italian salami made of lamb and enriched with pequi (Caryocar brasiliense, Cambess)

The purpose of this study was to evaluate and compare the inclusion of different levels of pequi (Caryocar Brasiliense, Cambess) pulp in the processing of Italian salami made of lamb for the evaluation of their physicochemical and microbiological characteristics. Six formulations of Italian salamis were processed: no pequi pulp (control - treatment 1); 4% pequi pulp (treatment 2); 8% pequi pulp (treatment 3), 12% pequi pulp (Treatment 4), 16% pequi pulp (treatment 5) and 20% pequi pulp (treatment 6). During the maturation period, all Italian salamis made of lamb enriched with pequi presented similar behaviors; and acidity (pH) and water activity (aw) results showed stable end values. For protein, the Italian salami made of lamb without pequi pump presented a higher content. For one of the lowest contents of fat, treatment 3 showed the highest content of humidity. The contents of fat and ashes did not present any significant difference when comparing the Italian salami formulations. A color tending to dark, yellowish and reddish shades was observed in the Italian salamis made of lamb and enriched with pequi. All Italian salamis were microbiologically satisfactory, showing ideal sanitary conditions for consumption. Pequi is a typical fruit from the Brazilian cerrado region, with characteristic color, odor and flavor, and it is an option for inclusion in Italian salamis, but additional studies should be conducted to inspect the sensory acceptability of these products.Keywords: Cerrado fruit, fermented stuffed meat sausages, physicochemical parameters, microbiological parameters

Physicochemical and microbiological meat quality, growth performance and carcass characteristics of feedlot-finished cull Santa Inês ewes and Moxotó goats

This study aimed at assessing the physicochemical and microbiological quality of feedlot-finished, culled Santa Inês ewes and Moxotó goats, and compares the intake, weight gain and carcass characteristics. Three Santa Inês ewes and Moxotó goats with similar ages were confined in double and/or individual pens and then euthanized after 40 days of confinement. The average intake, daily weight gain, hot carcass yield, cold carcass yield, weight loss by cooling and rib eye area (94.45%, 0.220 kg/day, 41.37%, 40.14%, 2.99%, 16.1 cm², respectively) of the Santa Inês ewes were higher than that of the Moxotó goats (84.01%; 0.06 kg/day; 41.55%; 39.81%; 4.12%; and 11.35 cm², respectively). The chevon (goat meat) showed higher protein levels (23.09 g/100 g), lower lipid levels (1.43 g/100 g) and higher red-color intensity (11.62), although it had lower tenderness (8.98 kgf shear strength). The superiority of the culled ewes was assessed relative to that of the culled goats, and the results showed that the Santa Inês sheep has better performance than the Moxotó goats. However, mutton and chevon derived from the culled animals are promising alternatives for small farmers because culled meats add value to the standard meat obtained from these animals, broaden sales alternatives and increase profitability.Key words: Performance parameters, physicochemical parameters, microbiological parameters, cull animals

Features of host cell invasion by different infective forms of Trypanosoma cruzi

Through its life cycle from the insect vector to mammalian hosts Trypanosoma cruzi has developed clever strategies to reach the intracellular milieu where it grows sheltered from the hosts' immune system. We have been interested in several aspects of in vitro interactions of different infective forms of the parasite with cultured mammalian cells. We have observed that not only the classically infective trypomastigotes but also amastigotes, originated from the extracellular differentiation of trypomastigotes, can infect cultured cells. Interestingly, the process of invasion of different parasite infective forms is remarkably distinct and also highly dependent on the host cell type.Universidade Federal de São Paulo (UNIFESP)UNIFESPSciEL

The influences of moisture content variation, number and width of gaps on the withdrawal resistance of self tapping screws inserted in cross laminated timber

A large experimental campaign comprised of 470 withdrawal tests was carried out, aiming to quantify the withdrawal resistance of self-tapping screws (STS) inserted in the side face of cross laminated timber (CLT) elements. In order to deeply understand the “CLT-STS” composite model, the experimental tests considered two main parameters: (i) simple and cyclic changes on moisture content (MC) and (ii) number and width of gaps. Regarding (i), three individual groups of test specimens were stabilized with 8%, 12% and 18% of moisture content and one group was submitted to a six month RH cycle (between 30% and 90% RH). Concerning (ii), different test configurations with 0 (REF), 1, 2 and 3 gaps, and widths equal to 0mm (GAP0) or 4mm (GAP4), were tested. The influences of MC and number of gaps were modeled by means of least square method. Moreover, a revision of a prediction model developed by Uibel and Blaß (2007) was proposed. The main findings of the experimental campaign were: the decrease of withdrawal resistance for specimens tested with MC=18% in most configurations; the unexpected increase of withdrawal resistance as the number of gaps with 0mm increased; and, the surprising increase of withdrawal resistance for REF specimens submitted to the RH cycle.The development of the present work was possible only thanks to the financial support of the 391 Portuguese Science Foundation (Fundação de Ciência e Tecnologia, FCT), through PhD grant SFRH / BD17392 / 79972 / 2011. Further, the valuable partnerships with the Institute of Timber Engineering and Wood393 Technology, at Graz University of Technology (Austria), and Rusticasa are gratefully acknowledged

Towards 100 % recycling of reclaimed asphalt in road surface courses: binder design methodology and case studies

Reclaimed Asphalt (RA) has shown great potential to be reused in new asphalt mixtures, however its incorporation in top asphalt pavement layers is still very limited (10-30%). In fact, despite the advantages that its use implies, RA content in road pavement surface courses is still restricted in most countries due to mainly legislation limitations, but also some technical issues. This paper aims at being a step further to improve the latter by providing a methodology that allows producing fundamental inputs for confidently performing mix design of asphalt mixtures incorporating up to 100% RA. The methodology consists in an advanced preliminary binder’s blend design that can be used with any type of RA and also in presence of rejuvenators. This procedure includes in the production of blending charts and laws that considers the uncertainties on accounting the extent of final binder content, Degree of Blending and Replaced Virgin Binder. The description of the methodology is accompanied with results of two extreme case studies consisting in the preliminary design of binders for asphalt mixtures with high content of two types of RA corresponding to extreme cases: the short-term aged RA (STA-RA), having a very soft residual binder (Pen> 20dmm) and the long-term aged RA, having a much harder residual binder (Pen <10dmm). As a result, the proposed methodology allowed assessing the feasibility of using up to 90% of RA and determining whether the use of rejuvenating agents was needed

New insights into the distribution and conservation status of the Golden-White Tassel-Ear Marmoset Mico chrysoleucos (Primates, Callitrichidae)

Among the 13 Mico species recognized by the IUCN Red List of Threatened Species, six are listed as "Data Deficient". The geographic range of most of the Mico species has been estimated from only a few records. We report new localities and the geographic extension of Mico chrysoleucos. In addition, we confirmed the presence of the species in two distinct protected areas. We modeled the habitat suitability of M. chrysoleucos using the maximum entropy method and including new records obtained by the authors in the state of Amazonas, Brazil. From the total area of occurrence calculated for the species, 22.8% is covered by protected areas and indigenous lands. The annual mean deforestation rate estimated between 2000 and 2015 was 2.95%, and the total area deforested by 2015 was 3354 km2 or 8.6% of the total distribution limits of the species. The habitat lost between 2000 and 2015 was 3.2% (1131 km2 ) of the total potential distribution, while the habitat loss area legally protected was 31 km2, and the habitat loss in settlements was equal to 691 km2. Our results extend the geographic distribution of the species about 100 km farther south, with the Maracanã River being a possible geographic barrier for the species. The significantly low rate of habitat loss inside protected areas and indigenous land, when compared to unprotected areas, points out the importance of these areas to M. chrysoleucos conservation. The species is relatively wide-ranging, legally protected, and resilient to regional anthropic threats. However, the hydroelectric schemes and the improvement of the road system in southern Amazonia pose an imminent threat to the species

Evaluation of the impact of strain correction on the orientation of cardiac diffusion tensors with in vivo and ex vivo porcine hearts

Purpose To evaluate the importance of strain-correcting stimulated echo acquisition mode echo-planar imaging cardiac diffusion tensor imaging. Methods Healthy pigs (n = 11) were successfully scanned with a 3D cine displacement-encoded imaging with stimulated echoes and a monopolar-stimulated echo-planar imaging diffusion tensor imaging sequence at 3 T during diastasis, peak systole, and strain sweet spots in a midventricular short-axis slice. The same diffusion tensor imaging sequence was repeated ex vivo after arresting the hearts in either a relaxed (KCl-induced) or contracted (BaCl2-induced) state. The displacement-encoded imaging with stimulated echoes data were used to strain-correct the in vivo cardiac diffusion tensor imaging in diastole and systole. The orientation of the primary (helix angles) and secondary (E2A) diffusion eigenvectors was compared with and without strain correction and to the strain-free ex vivo data. Results Strain correction reduces systolic E2A significantly when compared without strain correction and ex vivo (median absolute E2A = 34.3° versus E2A = 57.1° (P = 0.01), E2A = 60.5° (P = 0.006), respectively). The systolic distribution of E2A without strain correction is closer to the contracted ex vivo distribution than with strain correction, root mean square deviation of 0.027 versus 0.038. Conclusions The current strain-correction model amplifies the contribution of microscopic strain to diffusion resulting in an overcorrection of E2A. Results show that a new model that considers cellular rearrangement is required

The Second Transmembrane Domain of P2X7 Contributes to Dilated Pore Formation

Activation of the purinergic receptor P2X7 leads to the cellular permeability of low molecular weight cations. To determine which domains of P2X7 are necessary for this permeability, we exchanged either the C-terminus or portions of the second transmembrane domain (TM2) with those in P2X1 or P2X4. Replacement of the C-terminus of P2X7 with either P2X1 or P2X4 prevented surface expression of the chimeric receptor. Similarly, chimeric P2X7 containing TM2 from P2X1 or P2X4 had reduced surface expression and no permeability to cationic dyes. Exchanging the N-terminal 10 residues or C-terminal 14 residues of the P2X7 TM2 with the corresponding region of P2X1 TM2 partially restored surface expression and limited pore permeability. To further probe TM2 structure, we replaced single residues in P2X7 TM2 with those in P2X1 or P2X4. We identified multiple substitutions that drastically changed pore permeability without altering surface expression. Three substitutions (Q332P, Y336T, and Y343L) individually reduced pore formation as indicated by decreased dye uptake and also reduced membrane blebbing in response to ATP exposure. Three others substitutions, V335T, S342G, and S342A each enhanced dye uptake, membrane blebbing and cell death. Our results demonstrate a critical role for the TM2 domain of P2X7 in receptor function, and provide a structural basis for differences between purinergic receptors. © 2013 Sun et al

Phosphorylated c-Src in the nucleus is associated with improved patient outcome in ER-positive breast cancer

Elevated c-Src protein expression has been shown in breast cancer and &lt;i&gt;in vitro&lt;/i&gt; evidence suggests a role in endocrine resistance. To investigate whether c-Src is involved in endocrine resistance, we examined the expression of both total and activated c-Src in human breast cancer specimens from a cohort of oestrogen receptor (ER)-positive tamoxifen-treated breast cancer patients. Tissue microarray technology was employed to analyse 262 tumour specimens taken before tamoxifen treatment. Immunohistochemistry using total c-Src and activated c-Src antibodies was performed. Kaplan–Meier survival curves were constructed and log-rank test were performed. High level of nuclear activated Src was significantly associated with improved overall survival (&lt;i&gt;P&lt;/i&gt;=0.047) and lower recurrence rates on tamoxifen (&lt;i&gt;P&lt;/i&gt;=0.02). Improved patient outcome was only seen with activated Src in the nucleus. Nuclear activated Src expression was significantly associated with node-negative disease and a lower NPI (&lt;i&gt;P&lt;/i&gt;&#60;0.05). On subgroup analysis, only ER-positive/progesterone receptor (PgR)-positive tumours were associated with improved survival (&lt;i&gt;P&lt;/i&gt;=0.004). This shows that c-Src activity is increased in breast cancer and that activated Src within the nucleus of ER-positive tumours predicts an improved outcome. In ER/PgR-positive disease, activated Src kinase does not appear to be involved in &lt;i&gt;de novo&lt;/i&gt; endocrine resistance. Further study is required in ER-negative breast cancer as this may represent a cohort in which it is associated with poor outcome