Módulos multimídia para o ensino de sistemas de telecomunicações

A tecnologia multimídia fornece elementos para uma concepção integridade transmissão do conhecimento, onde se exige maior comprometimento por parte do aluno, aumentando a sua responsabilidade, encorajando-o ao controle do seu aprendizado. A capacidade de combinar aplicações práticas, visualizações de fenômenos complexos, laboratórios virtuais, animações e a utilização auto assistida de aplicativos de simulação são algumas das vantagens da educação baseada em técnicas multimídia. Este trabalho descreve o desenvolvimento de um ambiente multimídia na forma de CD-ROM, para a área de Sistemas de Telecomunicações. OCD-ROM foi concebido para ser utilizado em atividades de extensão, de maneira a facilitar a aprendizagem e atualização do estudante, de forma mais descentralizada, considerando seus interesses e sua disponibilidade

Catalyzed and non-catalyzed synthesis of bioactive monastrol

The bioactive 3,4-dihydropyrimidin-2(1H)-thione derivative known as Monastrol was synthesized under catalyzed and non-catalyzed conditions through the Biginelli multicomponent reaction under solvent-free conditions. The use of two Lewis acids (FeCl3 and CuCl2) and two Brønsted acids (HCl and CF3COOH) as catalysts improved the reaction yields of the transformation compared with the non-catalyzed reaction. The experiments investigated catalysis and its role, the importance of multicomponent reactions and their green features, and the application of these concepts to the synthesis of a biologically important structure

A Taxonomically-informed Mass Spectrometry Search Tool for Microbial Metabolomics Data

MicrobeMASST, a taxonomically-informed mass spectrometry (MS) search tool, tackles limited microbial metabolite annotation in untargeted metabolomics experiments. Leveraging a curated database of >60,000 microbial monocultures, users can search known and unknown MS/MS spectra and link them to their respective microbial producers via MS/MS fragmentation patterns. Identification of microbial-derived metabolites and relative producers, without a priori knowledge, will vastly enhance the understanding of microorganisms’ role in ecology and human health

Probing the mechanism of the ugi four-component reaction with charge-tagged reagents by ESI-MS(/MS)

The mechanism of the Ugi four-component reaction has been investigated by electrospray ionization (tandem) mass spectrometry using charge-tagged reagents (a carboxylic acid or an amine) to favour detection. Key intermediates were transferred directly via ESI(+) from the reaction solution to the gas phase and characterized by MS measurements and MS/MS collision induced dissociation. The Mumm rearrangement (final step) was also investigated by both travelling wave ion mobility mass spectrometry and DFT calculations. The data seem to consolidate the amazingly selective mechanism of this intricate four-component reaction. © 2013 The Royal Society of Chemistry.The mechanism of the Ugi four-component reaction has been investigated by electrospray ionization (tandem) mass spectrometry using charge-tagged reagents (a carboxylic acid or an amine) to favour detection. Key intermediates were transferred directly via503338340FAPDF - FUNDAÇÃO DE APOIO À PESQUISA NO DISTRITO FEDERALCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TEsem informaçãosem informaçãosem informaçãosem informaçãoDe Graaff, C., Ruijter, E., Orru, R.V.A., (2012) Chem. Soc. Rev., 41, pp. 3969-4009Hernandez, J.G., Juaristi, E., (2012) Chem. Commun., 48, pp. 5396-5409Domling, A., Wang, W., Wang, K., (2012) Chem. Rev., 112, pp. 3083-3135Van Berkel, S.S., Bogels, B.G.M., Wijdeven, M.A., Westermann, B., Rutjes, F., (2012) Eur. J. Org. Chem., pp. 3543-3559Vercillo, O.E., Andrade, C.K.Z., Wessjohann, L.A., (2008) Org. Lett., 10, pp. 205-208Basso, A., Banfi, L., Riva, R., (2010) Eur. J. Org. Chem., pp. 1831-1841Domling, A., (2006) Chem. Rev., 106, pp. 17-89Ugi, I., (1959) Angew. Chem., Int. Ed., 71, p. 386Cheron, N., Ramozzi, R., El Kaim, L., Grimaud, L., Fleurat-Lessard, P., (2012) J. Org. Chem., 77, pp. 1361-1366Coelho, F., Eberlin, M.N., (2011) Angew. Chem., Int. Ed., 50, pp. 5261-5263Li, R.M., Smith, R.L., Kenttamaa, H.I., (1996) J. Am. Chem. Soc., 118, pp. 5056-5061Dos Santos, M.R., Diniz, J.R., Arouca, A.M., Gomes, A.F., Gozzo, F.C., Tamborim, S.M., Parize, A.L., Neto, B.A.D., (2012) ChemSusChem, 5, pp. 716-726Oliveira, F.F.D., Dos Santos, M.R., Lalli, P.M., Schmidt, E.M., Bakuzis, P., Lapis, A.A.M., Monteiro, A.L., Neto, B.A.D., (2011) J. Org. Chem., 76, pp. 10140-10147Alvim, H.G.O., De Lima, T.B., De Oliveira, H.C.B., Gozzo, F.C., De MacEdo, J.L., Abdelnur, P.V., Silva, W.A., Neto, B.A.D., (2013) ACS Catal., 3, pp. 1420-1430Vikse, K.L., Henderson, M.A., Oliver, A.G., McIndoe, J.S., (2010) Chem. Commun., 46, pp. 7412-7414Limberger, J., Leal, B.C., Back, D.F., Dupont, J., Monteiro, A.L., (2012) Adv. Synth. Catal., 354, pp. 1429-1436Vikse, K.L., Ahmadi, Z., Manning, C.C., Harrington, D.A., McIndoe, J.S., (2011) Angew. Chem., Int. Ed., 50, pp. 8304-8306Gozzo, F.C., Santos, L.S., Augusti, R., Consorti, C.S., Dupont, J., Eberlin, M.N., (2004) Chem.-Eur. J., 10, pp. 6187-6193Lapthorn, C., Pullen, F., Chowdhry, B.Z., (2013) Mass Spectrom. Rev., 32, pp. 43-71Lalli, P.M., Corilo, Y.E., De Sa, G.F., Daroda, R.J., De Souza, V., Souza, G., Campuzano, I., Eberlin, M.N., (2011) ChemPhysChem, 12, pp. 1444-144

Fluorescent Peptoids as Selective Live Cell Imaging Probes

This paper describes the synthesis of fluorescent peptoids using the Ugi multicomponent reaction (4CR). The four synthesized structures had their photophysical properties evaluated and their potential as biomarkers established. The peptidomimetics were used at very low concentrations (10 nM) to follow their internalization in breast cancer cells and had their localization precisely determined. One of the new peptoids displayed mitochondrial affinity and stained this important organelle selectively. Co-staining experiments using MitoTracker Red confirmed the localization inside live cells

Morita–Baylis–Hillman Reaction: ESI-MS(/MS) Investigation with Charge Tags and Ionic Liquid Effect Origin Revealed by DFT Calculations

The use of a charge-tagged acrylate derivative bearing an imidazolium tag to study the Morita–Baylis–Hillman reaction via ESI-MS­(/MS) monitoring and the effect of such tag (imidazolium cations and ion pairs) over TSs is described. The ionic nature of the substrate was meant to facilitate ESI transfer to the gas phase for direct mass spectrometric analysis. The detection and characterization of charged intermediates has suggested major reaction pathways. DFT calculations considering the effect of a polar and protic solvent (methanol), of a polar and aprotic solvent (acetonitrile), and of no solvent (gas phase) were used to predict possible TSs through a common accepted intermediate. The controversial proton transfer step, which may proceed via Aggarwal’s or McQuade’s proposals, was evaluated. Calculations predicted the formation of electrostatic intermediate complexes with both the cation and anion when charge-tagged reagents are used. These complexes contribute to the positive ionic liquid effect, and based on the formation of these unique complexes, a rationale for the ionic liquid effect is proposed. These complexes also pointed to a plausible explanation for the positive ionic liquid effect observed in several reactions that are difficult to be carried out in organic solvents but have shown a beneficial effect when performed in ionic liquids

Development of TiO2-based photocatalysts with high photocatalytic activity under simulated solar light: Metoprolol degradation and optimization via Box-Behnken

The photocatalytic activity of N-doped TiO2 nanoparticles to degrade metoprolol (MET) has been investigated. The materials were synthesized via sol-gel method and characterized using different techniques. All materials revealed the formation of anatase phase, with a band gap energy close to 3.12 eV, which suggests that the N doping did not cause substantial variations as compared to pure TiO2. However, the TiO2-5%N photocatalyst exhibited a reduction in nanoparticle size and a surface area 0.4-fold larger than undoped TiO2. The influence of pH, catalyst concentration, and doping percentage was investigated through a Box-Behnken experimental design. Under the best conditions (0.5 g L−1 of TiO2-5%N, solution pH 6.0), 98% MET degradation and 42% mineralization could be achieved, and the process by-products were non-toxic to Artemia salina